##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805372_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27248453
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.29063319668093	35.0	35.0	37.0	33.0	37.0
2	35.50182753494299	37.0	35.0	37.0	33.0	37.0
3	37.18625534447772	39.0	37.0	39.0	33.0	39.0
4	37.282344799537796	39.0	37.0	39.0	34.0	39.0
5	37.261620100047516	39.0	37.0	39.0	34.0	39.0
6	37.186985587768966	39.0	37.0	39.0	33.0	39.0
7	37.07892558157339	39.0	37.0	39.0	33.0	39.0
8	37.958589906003105	40.0	38.0	40.0	33.0	40.0
9	37.86191458282054	40.0	38.0	40.0	33.0	40.0
10	37.78370148206212	40.0	37.0	40.0	33.0	40.0
11	37.826126496061995	40.0	37.0	40.0	33.0	40.0
12	37.75029066787755	40.0	37.0	40.0	33.0	40.0
13	37.76502218309421	40.0	37.0	40.0	33.0	40.0
14	37.63267635781011	40.0	37.0	40.0	33.0	40.0
15	37.59625755634641	40.0	37.0	40.0	32.0	40.0
16	37.54313916463441	40.0	37.0	40.0	32.0	40.0
17	37.453145284981865	40.0	37.0	40.0	32.0	40.0
18	37.44720586522838	40.0	37.0	40.0	32.0	40.0
19	37.42466278727824	40.0	37.0	40.0	32.0	40.0
20	37.15086258291434	39.0	37.0	40.0	32.0	40.0
21	37.154995588189905	39.0	37.0	40.0	32.0	40.0
22	37.05031647117728	39.0	36.0	40.0	31.0	40.0
23	36.83562578763646	39.0	36.0	40.0	31.0	40.0
24	36.87800290900918	39.0	36.0	40.0	31.0	40.0
25	36.83280515044285	39.0	36.0	40.0	31.0	40.0
26	36.76892049614707	39.0	36.0	40.0	30.0	40.0
27	36.665785686989274	39.0	36.0	40.0	30.0	40.0
28	36.54666586026003	39.0	36.0	40.0	30.0	40.0
29	36.435414737122876	39.0	36.0	40.0	30.0	40.0
30	36.328118957799184	39.0	35.0	40.0	30.0	40.0
31	36.179768921193435	38.0	35.0	40.0	30.0	40.0
32	36.037216351328276	38.0	35.0	40.0	29.0	40.0
33	35.95346458751255	38.0	35.0	40.0	29.0	40.0
34	35.83986430348908	38.0	35.0	40.0	29.0	40.0
35	35.70220720420348	38.0	34.0	40.0	28.0	40.0
36	35.83348177601128	38.0	35.0	40.0	29.0	40.0
37	35.82549497397155	38.0	35.0	40.0	29.0	40.0
38	35.863069400673865	38.0	35.0	40.0	29.0	40.0
39	35.852743933756535	38.0	35.0	40.0	29.0	40.0
40	35.831041380587735	38.0	35.0	40.0	29.0	40.0
41	35.77041459931689	38.0	35.0	40.0	28.0	40.0
42	35.66496835618521	38.0	34.0	40.0	28.0	40.0
43	35.56570584025449	38.0	34.0	40.0	28.0	40.0
44	35.65142692687912	38.0	34.0	40.0	28.0	40.0
45	35.60534849446315	38.0	34.0	40.0	28.0	40.0
46	35.59677787946347	38.0	34.0	40.0	28.0	40.0
47	35.596041947775895	38.0	34.0	40.0	28.0	40.0
48	35.501643928189246	38.0	34.0	40.0	28.0	40.0
49	35.359713338588435	38.0	34.0	40.0	27.0	40.0
50	35.18853573081745	38.0	34.0	40.0	27.0	40.0
51	35.06167517106384	38.0	34.0	40.0	26.0	40.0
52	34.84120992116507	37.0	33.0	40.0	26.0	40.0
53	34.462948447018256	37.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	8.0
11	11.0
12	47.0
13	140.0
14	297.0
15	836.0
16	1988.0
17	4293.0
18	8678.0
19	15792.0
20	27534.0
21	44254.0
22	66190.0
23	95149.0
24	131483.0
25	174628.0
26	228726.0
27	293980.0
28	369992.0
29	461076.0
30	570198.0
31	703875.0
32	870045.0
33	1085323.0
34	1383413.0
35	1827837.0
36	2530264.0
37	3732808.0
38	5737386.0
39	6882202.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.249629437678536	35.2009451692542	16.160902785930638	20.388522607136633
2	28.318807676898206	28.9807645226685	25.895950863705913	16.80447693672738
3	24.758117460833464	24.3781802952263	26.395351692075874	24.468350551864358
4	22.154083389614815	29.14563993779757	23.869325719151835	24.830950953435778
5	22.7757957488449	30.689129397547816	22.837634121834366	23.69744073177292
6	25.630897284333905	24.64356783851179	27.45098593303627	22.274548944118038
7	22.028523967947834	25.847628854379366	27.984682286366862	24.13916489130594
8	24.877460015803464	24.619434358346876	26.57738404451805	23.925721581331608
9	26.076324406380063	25.120838970197685	25.69482017933275	23.108016444089504
10	22.56001102154313	25.920073334071482	29.209716969987248	22.310198674398137
11	22.47748890551695	29.431854351511262	25.823106361304255	22.26755038166754
12	22.468842543097768	25.12510710241055	29.394956843972025	23.01109351051966
13	22.89318222946455	25.51117305631993	25.37168256854802	26.2239621456675
14	22.814227288426245	28.8482028686179	25.51956252342105	22.8180073195348
15	22.715359290305397	28.634242098074335	25.427160947449018	23.223237664171247
16	23.21064612365333	25.304691609464946	25.018884558327038	26.465777708554683
17	26.45812222807658	25.295307590489635	25.276367065682592	22.970203115751193
18	22.93497542777933	25.061664968649776	25.150359912175563	26.852999691395325
19	23.387698376858314	28.671620366851652	24.891772754952363	23.04890850133767
20	26.697787210158314	25.198065372738775	25.179378073316677	22.924769343786235
21	23.29456648419637	28.37762936486706	25.057327107707728	23.27047704322884
22	26.907105515311276	25.1270631767609	24.946953869271038	23.018877438656794
23	23.078458802780474	25.229931401977208	28.77160035470638	22.920009440535946
24	23.127889131907782	28.36995186479027	25.06903786427802	23.43312113902393
25	23.34740618118761	25.150704885888388	24.88521825440879	26.61667067851522
26	23.13231874117771	27.410767136027868	26.455311059310414	23.00160306348401
27	23.323500236875834	24.77094020713763	28.62136797270656	23.284191583279977
28	25.778193719841635	25.108203390482387	25.992202199515695	23.121400690160282
29	25.638226140764765	25.177700913883072	26.19163737478968	22.992435570562485
30	25.549633955366204	24.81368391812922	26.260852313340504	23.375829813164074
31	25.088620627380205	25.47704267834948	26.01955054108943	23.41478615318088
32	24.22347793469229	25.835631843026096	26.471689970803112	23.469200251478497
33	24.137784996454663	25.322534824270576	26.583560541950767	23.956119637323997
34	24.25862855406874	25.569682799974004	26.350171145495853	23.821517500461404
35	23.934000950439277	25.771775006823322	26.647175162567944	23.647048880169454
36	23.92291775243167	25.366904315632155	26.72164911527271	23.98852881666346
37	24.30994522881721	25.58406159791897	26.37135032950311	23.73464284376071
38	24.0902373430154	25.72122534809591	26.627427252475584	23.561110056413114
39	24.167555493884368	25.37670303704948	26.510338036438252	23.945403432627902
40	24.19137335980138	25.71623423905937	26.2414346972285	23.85095770391075
41	24.015546864256844	25.814305861694244	26.57987959903632	23.590267675012598
42	24.14120170418482	25.469049564024793	26.581655846664027	23.80809288512636
43	24.298373929705296	25.68480860179475	26.211069670634146	23.80574779786581
44	24.148115858173675	25.587603083375043	26.614648545368798	23.649632513082487
45	24.10645110751792	25.220947405711435	26.649410151835042	24.0231913349356
46	24.191586215922058	25.58175320998957	26.244902784022273	23.981757790066098
47	24.007828994915783	25.584689156481655	26.629985195856808	23.777496652745754
48	24.08375624113413	25.240199874833262	26.612398876369237	24.06364500766337
49	24.152409679918343	25.749116839770686	26.146904560049705	23.95156892026127
50	24.06445239294869	25.699356216663016	26.495628944512923	23.74056244587537
51	24.03963997515749	25.356367937658696	26.548068618794616	24.055923468389196
52	24.40391019629628	25.530051192264015	26.072390238080672	23.99364837335903
53	24.224252290579578	25.64955522429108	26.231760753537092	23.89443173159225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5637.0
1	3717.0
2	1797.0
3	1759.0
4	1721.0
5	1926.0
6	2131.0
7	2588.0
8	3810.5
9	4576.0
10	5949.0
11	7322.0
12	10120.0
13	12918.0
14	16658.5
15	20399.0
16	27778.5
17	35158.0
18	47138.5
19	59119.0
20	80365.5
21	101612.0
22	238303.5
23	374995.0
24	314660.5
25	280116.0
26	305906.0
27	347850.5
28	389795.0
29	430153.5
30	470512.0
31	510074.0
32	549636.0
33	616708.5
34	683781.0
35	765675.5
36	847570.0
37	935327.0
38	1023084.0
39	1111088.5
40	1199093.0
41	1287009.5
42	1457775.5
43	1540625.0
44	1630439.5
45	1720254.0
46	1794911.0
47	1869568.0
48	1946487.0
49	2023406.0
50	2046934.5
51	2070463.0
52	2050658.0
53	2030853.0
54	1954519.5
55	1878186.0
56	1773001.0
57	1667816.0
58	1536277.0
59	1262672.5
60	1120607.0
61	966601.0
62	812595.0
63	684623.5
64	556652.0
65	456155.5
66	355659.0
67	285657.5
68	215656.0
69	170653.5
70	125651.0
71	94709.5
72	63768.0
73	47557.0
74	31346.0
75	23944.5
76	12089.5
77	7636.0
78	5694.0
79	3752.0
80	2922.0
81	2092.0
82	1480.5
83	869.0
84	609.0
85	349.0
86	243.5
87	138.0
88	105.5
89	73.0
90	58.5
91	44.0
92	39.0
93	22.5
94	11.0
95	10.0
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.7248453E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.511880385570805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.22607553107832	33.32656390675209
2	14.148572787197155	12.878563046349182
3	4.625918863310863	6.316027979410789
4	2.226863226652533	4.053949312257453
5	1.2703913561228555	2.890894972136324
6	0.8313941735939582	2.2702987309121228
7	0.5888415703614154	1.8759500981438286
8	0.42808412721117445	1.5586330874077157
9	0.3418895722949463	1.4004033587425402
>10	2.051024444373698	17.425224805950975
>50	0.16409960594632747	5.14883267997038
>100	0.09068352802823679	7.660630105522836
>500	0.005151514768419982	1.5427951447706378
>1k	9.369068209941143E-4	0.6368376256234932
>5k	4.852815917690975E-5	0.16533269706473483
>10k+	2.426407958020756E-5	0.8490624489848343
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	200321	0.7351646715503446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.13813345660394E-4	0.0	0.0	0.0	2.935946492081587E-5
2	9.835420748473317E-4	0.0	0.0	3.6699331151019838E-6	4.036926426612182E-5
3	9.908819410775357E-4	0.0	0.0	3.6699331151019838E-6	6.605879607183571E-5
4	0.0010092316066530455	3.6699331151019838E-6	0.0	7.3398662302039676E-6	7.339866230203969E-5
5	0.0012844765902856943	3.6699331151019838E-6	0.0	1.1009799345305952E-5	9.17483278775496E-5
6	0.0013395255870122242	3.6699331151019838E-6	0.0	1.4679732460407935E-5	1.1743785968326348E-4
7	0.001346865453242428	3.6699331151019838E-6	0.0	1.4679732460407935E-5	1.6881692329469126E-4
8	0.00135053538635753	3.6699331151019838E-6	0.0	1.4679732460407935E-5	2.0918618756081308E-4
9	0.00135053538635753	3.6699331151019838E-6	0.0	1.4679732460407935E-5	2.2753585313632302E-4
10	0.001365215118817938	7.3398662302039676E-6	0.0	1.4679732460407935E-5	2.6423518428734285E-4
11	0.0014202641155444677	7.3398662302039676E-6	0.0	1.834966557550992E-5	2.825848498628528E-4
12	0.0014422837142350796	7.3398662302039676E-6	0.0	2.2019598690611904E-5	3.0460444855346465E-4
13	0.0015413719083428332	7.3398662302039676E-6	0.0	2.5689531805713887E-5	3.486436459346885E-4
14	0.001563391507033445	7.3398662302039676E-6	0.0	3.3029398035917857E-5	3.5231357904979047E-4
15	0.001596420905069363	7.3398662302039676E-6	0.0	4.036926426612182E-5	3.743331777404024E-4
16	0.001607430704414669	7.3398662302039676E-6	0.0	4.036926426612182E-5	4.330521075820341E-4
17	0.0016221104368750769	7.3398662302039676E-6	0.0	4.036926426612182E-5	4.58741639387748E-4
18	0.0016588097680260967	7.3398662302039676E-6	0.0	4.036926426612182E-5	4.6241157250284995E-4
19	0.0016918391660620145	7.3398662302039676E-6	0.0	4.036926426612182E-5	5.064507698840738E-4
20	0.0016955090991771166	7.3398662302039676E-6	0.0	4.036926426612182E-5	5.358102348048897E-4
21	0.0017468881627885444	7.3398662302039676E-6	0.0	4.036926426612182E-5	5.614997666106036E-4
22	0.0017909273601697682	7.3398662302039676E-6	0.0	4.036926426612182E-5	5.945291646465214E-4
23	0.001834966557550992	7.3398662302039676E-6	0.0	4.036926426612182E-5	6.238886295673372E-4
24	0.0018863456211624197	1.1009799345305952E-5	0.0	4.036926426612182E-5	6.348984289126432E-4
25	0.0019487344841191535	1.1009799345305952E-5	0.0	4.403919738122381E-5	7.046271580995809E-4
26	0.0020147932801909893	1.1009799345305952E-5	0.0	4.403919738122381E-5	7.266467567901929E-4
27	0.002113881474298743	1.1009799345305952E-5	0.0	4.403919738122381E-5	7.413264892506008E-4
28	0.0022019598690611905	1.1009799345305952E-5	0.0	4.770913049632579E-5	8.037153522073345E-4
29	0.0023414173274350658	1.1009799345305952E-5	0.0	5.1379063611427775E-5	8.220650177828444E-4
30	0.0024478453877730235	1.1009799345305952E-5	0.0	5.1379063611427775E-5	8.367447502432523E-4
31	0.002561613314341185	1.1009799345305952E-5	0.0	5.504899672652976E-5	8.514244827036603E-4
32	0.0026863910402546523	1.1009799345305952E-5	0.0	5.504899672652976E-5	8.734440813942722E-4
33	0.0028735576291248534	1.1009799345305952E-5	0.0	5.871892984163174E-5	8.807839476244762E-4
34	0.0030680640842252584	1.1009799345305952E-5	0.0	5.871892984163174E-5	9.101434125452921E-4
35	0.003280920204901174	1.1009799345305952E-5	0.0	5.871892984163174E-5	9.284930781208019E-4
36	0.0035524952554187206	1.1009799345305952E-5	0.0	6.238886295673373E-5	9.541826099265158E-4
37	0.0037433317774040234	1.1009799345305952E-5	0.0	6.238886295673373E-5	9.908819410775357E-4
38	0.003963527764310143	1.1009799345305952E-5	0.0	6.238886295673373E-5	0.0010092316066530455
39	0.004147024420065242	1.1009799345305952E-5	0.0	6.605879607183571E-5	0.0010679505364946773
40	0.004356210607626055	1.1009799345305952E-5	0.0	6.97287291869377E-5	0.0010899701351852892
41	0.0046131059256831934	1.1009799345305952E-5	0.0	7.339866230203969E-5	0.001111989733875901
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	105640	0.0	42.43303	12
AAGCAGT	106890	0.0	42.143467	1
GGTATCA	105800	0.0	42.13563	8
GTATCAA	106300	0.0	41.963974	9
TGGTATC	106720	0.0	41.831852	7
ACGCAGA	107530	0.0	41.76588	15
ATCAACG	107660	0.0	41.6456	11
GTGGTAT	107655	0.0	41.571133	6
AGTGGTA	107910	0.0	41.414097	5
CAACGCA	108355	0.0	41.389324	13
CGCAGAG	109210	0.0	41.084656	16
AACGCAG	110270	0.0	40.69611	14
TATCAAC	109935	0.0	40.674763	10
AGAGTAC	109065	0.0	40.11365	19
CAGTGGT	111910	0.0	40.066128	4
CAGAGTA	112155	0.0	39.186577	18
GCAGTGG	114990	0.0	39.142143	3
GCAGAGT	113095	0.0	39.045807	17
AGCAGTG	115675	0.0	38.955048	2
GAGTACA	76500	0.0	38.192875	20
>>END_MODULE