FastQCFastQC Report
Thu 26 May 2016
SRR805371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26144539
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7255252.7750537119816876No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG467790.17892455476074756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC417470.1596777055430199No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG393580.15054004203325214No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG368350.14088984319057987No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA314760.12039225476494346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC3218600.045.83909212
GTATCAA3209850.045.7187849
GGTATCA3214300.045.661348
ACGCAGA3249050.045.65106615
ATCAACG3233500.045.55591211
TGGTATC3231650.045.554367
AAGCAGT3266550.045.533111
GTGGTAT3253350.045.4014786
AGTGGTA3250350.045.343615
CGCAGAG3267800.045.34310516
AGAGTAC3184350.045.29968319
CAACGCA3261400.045.26491513
TATCAAC3249900.045.17851310
AACGCAG3282950.045.0329314
CAGTGGT3293200.044.9184534
CAGAGTA3226800.044.91713318
GCAGAGT3253550.044.80496617
AGCAGTG3337400.044.6185952
GTACATG1970850.044.559222
GCAGTGG3342500.044.4949723