##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805371_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26144539
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.93873470096375	35.0	35.0	37.0	32.0	37.0
2	35.402953098541914	36.0	35.0	37.0	33.0	37.0
3	37.288727982543506	39.0	37.0	39.0	34.0	39.0
4	37.363416390704	39.0	37.0	39.0	34.0	39.0
5	37.333535427800044	39.0	37.0	39.0	34.0	39.0
6	37.31931991610179	39.0	37.0	39.0	34.0	39.0
7	37.09228374613911	39.0	37.0	39.0	33.0	39.0
8	38.05476994641214	40.0	38.0	40.0	34.0	40.0
9	38.01905040283938	40.0	38.0	40.0	34.0	40.0
10	37.68370568706528	40.0	37.0	40.0	33.0	40.0
11	37.84207290861009	40.0	37.0	40.0	33.0	40.0
12	37.89117819212647	40.0	38.0	40.0	33.0	40.0
13	37.898801849212184	40.0	38.0	40.0	33.0	40.0
14	37.79981092801063	40.0	38.0	40.0	33.0	40.0
15	37.75737835729289	40.0	37.0	40.0	33.0	40.0
16	37.685158074502674	40.0	37.0	40.0	33.0	40.0
17	37.60632172554276	40.0	37.0	40.0	33.0	40.0
18	37.59162347440894	40.0	37.0	40.0	33.0	40.0
19	37.55040588017253	40.0	37.0	40.0	33.0	40.0
20	37.34639627801431	40.0	37.0	40.0	32.0	40.0
21	37.19558241206701	39.0	37.0	40.0	32.0	40.0
22	37.22998095319256	39.0	37.0	40.0	32.0	40.0
23	36.70333804700094	39.0	36.0	40.0	30.0	40.0
24	36.89661076066402	39.0	36.0	40.0	31.0	40.0
25	36.93933130739081	39.0	36.0	40.0	31.0	40.0
26	36.917215981509564	39.0	36.0	40.0	31.0	40.0
27	36.88065014265503	39.0	36.0	40.0	31.0	40.0
28	36.782555737548094	39.0	36.0	40.0	30.0	40.0
29	36.68431981149104	39.0	36.0	40.0	30.0	40.0
30	36.5830259619418	39.0	36.0	40.0	30.0	40.0
31	36.377385541202315	39.0	35.0	40.0	30.0	40.0
32	36.216509420954026	38.0	35.0	40.0	30.0	40.0
33	36.09063999942779	38.0	35.0	40.0	30.0	40.0
34	35.972389645118625	38.0	35.0	40.0	29.0	40.0
35	35.83270364797788	38.0	35.0	40.0	29.0	40.0
36	35.91217603798636	38.0	35.0	40.0	29.0	40.0
37	35.82707019618896	38.0	35.0	40.0	29.0	40.0
38	35.800698149621226	38.0	35.0	40.0	29.0	40.0
39	35.77699006281962	38.0	35.0	40.0	29.0	40.0
40	35.73701081514576	38.0	34.0	40.0	29.0	40.0
41	35.67245523051678	38.0	34.0	40.0	29.0	40.0
42	35.56257756160856	38.0	34.0	40.0	28.0	40.0
43	35.43915128891735	38.0	34.0	40.0	28.0	40.0
44	35.5290514015183	38.0	34.0	40.0	28.0	40.0
45	35.478659922058675	38.0	34.0	40.0	28.0	40.0
46	35.45377220076438	38.0	34.0	40.0	28.0	40.0
47	35.43663592614886	38.0	34.0	40.0	28.0	40.0
48	35.31482184482197	38.0	34.0	40.0	27.0	40.0
49	35.12495144779566	38.0	34.0	40.0	27.0	40.0
50	34.9095441690519	37.0	33.0	40.0	26.0	40.0
51	34.703984988987564	37.0	33.0	40.0	26.0	40.0
52	34.38621969964741	37.0	33.0	40.0	25.0	40.0
53	33.92922674980041	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	14.0
12	49.0
13	92.0
14	267.0
15	567.0
16	1495.0
17	3396.0
18	6879.0
19	13364.0
20	23577.0
21	37875.0
22	57648.0
23	84111.0
24	117165.0
25	158893.0
26	207699.0
27	265832.0
28	335257.0
29	417365.0
30	518579.0
31	647596.0
32	812660.0
33	1036637.0
34	1358744.0
35	1851420.0
36	2625747.0
37	3915433.0
38	5582069.0
39	6064101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.534757679223183	38.906809563557424	27.25731365926934	20.301119097950053
2	26.291318427913378	43.20242173709776	13.667481381102187	16.83877845388668
3	37.32507962752757	24.282344393221088	18.10126390065627	20.291312078595077
4	21.950832638510093	22.648683918274482	19.345726463182235	36.05475698003319
5	20.348693086537114	35.446228369144315	22.47465904830068	21.730419496017888
6	33.75475849851474	22.52224833644992	21.04351887788115	22.679474287154193
7	22.287461255293124	22.09356607894291	34.00993224627139	21.609040419492576
8	33.194622402789356	23.738146616392815	22.145508092531294	20.92172288828654
9	33.54710519087753	23.009455244171644	22.16007710061363	21.2833624643372
10	21.19445288364044	23.50718060088954	34.34444187369301	20.953924641777007
11	20.90092313350792	35.565155690830885	22.445624304180694	21.0882968714805
12	20.776377047612122	23.084285402775702	34.79289499042228	21.346442559189892
13	21.0411359710722	23.20995600649145	22.76215312115467	32.98675490128168
14	21.305883419860645	35.3992051647956	22.466990142759833	20.827921272583925
15	21.002726420228715	35.27328594319449	22.421772286747913	21.302215349828888
16	21.112519903296057	22.93378360964789	22.677060781220888	33.27663570583517
17	33.31187442241762	22.882751920008992	22.569137669629594	21.23623598794379
18	21.07093951819154	22.847459654958918	22.574989752162008	33.506611074687534
19	21.190941634120993	35.08964912328345	22.562535908550537	21.156873334045017
20	33.62250143328211	22.981392022249846	22.503682317749032	20.89242422671901
21	21.377810486541758	34.93829437956431	22.48910183499506	21.194793298898865
22	33.264667623322794	22.82740192894585	22.757819520168248	21.150110927563116
23	21.381685100662896	22.9003999649793	34.617703528832536	21.10021140552526
24	21.3953743839201	34.717521697360965	22.558603921071242	21.328499997647693
25	21.17780695999268	22.99737623983349	22.52660871167015	33.298208088503685
26	21.351782106389408	30.358672608455635	27.073975180820742	21.215570104334216
27	21.536639066383998	22.805408808317484	34.34042573862175	21.317526386676775
28	28.839100968657355	22.784930344344566	27.10018333082867	21.275785356169408
29	29.001188355243134	22.92891069909475	26.866968279685484	21.202932665976633
30	28.905263925288565	22.90586573356677	26.87420497259485	21.314665368549814
31	26.454094294797088	23.88624255336841	27.60073910654917	22.058924045285327
32	24.44290182358924	24.915945161626297	27.927549229305594	22.713603785478874
33	24.04679615884602	24.237738519696215	28.571809967657106	23.143655353800654
34	23.19609842805031	24.534840717596897	28.84834190421181	23.420718950140984
35	23.293235348307345	24.686398180514868	28.909310659484184	23.1110558116936
36	23.281974870545625	24.411640993172607	29.273359916577608	23.03302421970416
37	23.51541559023091	24.157190149728784	29.244252499537282	23.083141760503025
38	23.76527656502186	24.360123542434618	28.76149776440885	23.113102128134674
39	24.103201819699326	24.23392127893324	28.620148934352983	23.04272796701445
40	23.37666386085446	24.64479867095763	28.69796250758141	23.280574960606497
41	23.786244614984415	24.862848031093606	28.437066723570837	22.913840630351142
42	23.860719058767877	24.82729184859599	28.45989367033781	22.852095422298323
43	23.710756575206776	24.761362210287967	28.84219148021696	22.685689734288296
44	24.003697292195515	24.533815646931085	28.404937643000704	23.057549417872696
45	23.782408249768718	24.276997196240487	28.61138228522599	23.329212268764806
46	23.35389428744565	24.528013287975742	28.46292680853925	23.655165616039355
47	23.667504712934505	24.578176727461134	28.34178105033713	23.41253750926723
48	23.82093637221907	24.199126249653897	28.463278698469306	23.516658679657727
49	23.293912353933646	25.02057121756861	28.3019180410869	23.38359838741085
50	23.806130220923	24.80588393622087	28.191076538010478	23.19690930484565
51	23.641476332782155	24.64773236200493	28.306071872217753	23.404719432995165
52	23.99846101704069	24.482630961670427	28.160611284827013	23.358296736461867
53	24.469136747830973	24.655278871048367	27.376914161691662	23.498670219429
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9586.0
1	5508.0
2	1430.0
3	1224.5
4	1019.0
5	1125.0
6	1231.0
7	1531.5
8	2381.0
9	2930.0
10	3983.0
11	5036.0
12	6655.5
13	8275.0
14	10866.5
15	13458.0
16	18766.5
17	24075.0
18	32838.0
19	41601.0
20	63039.0
21	84477.0
22	510052.0
23	935627.0
24	636399.0
25	326587.5
26	316004.0
27	338246.5
28	360489.0
29	385536.5
30	410584.0
31	433139.0
32	455694.0
33	514522.5
34	573351.0
35	652655.0
36	731959.0
37	810422.0
38	888885.0
39	972530.0
40	1056175.0
41	1130837.5
42	1280767.5
43	1356035.0
44	1461111.0
45	1566187.0
46	1634596.5
47	1703006.0
48	1798853.0
49	1894700.0
50	1917574.5
51	1940449.0
52	1943056.0
53	1945663.0
54	1896449.5
55	1847236.0
56	1764798.5
57	1682361.0
58	1547768.0
59	1267478.5
60	1121782.0
61	965722.5
62	809663.0
63	688791.5
64	567920.0
65	466541.0
66	365162.0
67	289575.5
68	213989.0
69	168683.5
70	123378.0
71	94518.5
72	65659.0
73	48201.0
74	30743.0
75	23209.0
76	11572.5
77	7470.0
78	5664.0
79	3858.0
80	3040.0
81	2222.0
82	1657.5
83	1093.0
84	743.0
85	393.0
86	280.0
87	167.0
88	123.5
89	80.0
90	63.0
91	46.0
92	34.5
93	14.5
94	6.0
95	6.0
96	6.0
97	4.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.6144539E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.572409078401364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.73687254743108	22.740899948538914
2	15.261708190089001	10.247446211857127
3	5.8404507841256335	5.882340087808073
4	2.900033291783314	3.894444160509293
5	1.7348183065214726	2.9121014931619187
6	1.0780208370979947	2.1715053922856726
7	0.7625038464888461	1.791936374072467
8	0.5948357777554536	1.5976056052220098
9	0.44878461219339355	1.3560102529783489
>10	3.16785333170687	20.95669267769899
>50	0.29225024868026056	6.733053449312579
>100	0.16424810504011647	10.221041160934183
>500	0.012959478370109963	2.9639855153592203
>1k	0.004354964990490721	2.258650485492147
>5k	1.584995615678376E-4	0.3337274198164372
>10k+	1.4717816430687811E-4	3.9385597649529425
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	725525	2.7750537119816876	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	46779	0.17892455476074756	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	41747	0.1596777055430199	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG	39358	0.15054004203325214	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	36835	0.14088984319057987	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	31476	0.12039225476494346	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9124452720317616E-4	0.0	0.0	0.0	2.294934326438114E-5
2	2.600925569963196E-4	0.0	0.0	0.0	2.6774233808444663E-5
3	2.9451657189289126E-4	0.0	0.0	0.0	4.589868652876228E-5
4	0.0014955322027288376	0.0	0.0	0.0	4.97235770728258E-5
5	0.0015758549041541715	0.0	0.0	0.0	7.267292033720694E-5
6	0.0015988042474185528	0.0	0.0	0.0	9.179737305752456E-5
7	0.001602629137962616	0.0	0.0	0.0	9.562226360158808E-5
8	0.0016141038095948068	0.0	0.0	0.0	1.1092182577784217E-4
9	0.0016141038095948068	0.0	0.0	0.0	1.2239649741003274E-4
10	0.0016217535906829339	0.0	0.0	0.0	1.2622138795409627E-4
11	0.001629403371771061	0.0	0.0	0.0	1.491707312184774E-4
12	0.0016561776055795056	0.0	0.0	0.0	1.5682051230660445E-4
13	0.0017097260731963949	0.0	0.0	0.0	1.644702933947315E-4
14	0.0017250256353726488	0.0	0.0	0.0	1.7594496502692207E-4
15	0.0017938736651657923	0.0	0.0	0.0	1.797698555709856E-4
16	0.0018015234462539194	0.0	3.824890544063523E-6	0.0	1.9506941774723969E-4
17	0.001820647898974237	0.0	3.824890544063523E-6	0.0	2.1036897992349378E-4
18	0.0018359474611504912	0.0	3.824890544063523E-6	0.0	2.2566854209974787E-4
19	0.0018550719138708087	0.0	3.824890544063523E-6	0.0	2.4096810427600196E-4
20	0.001870371476047063	0.0	3.824890544063523E-6	0.0	2.639174475403831E-4
21	0.001908620381487698	0.0	3.824890544063523E-6	0.0	2.7921700971663717E-4
22	0.0019200950531198886	0.0	3.824890544063523E-6	0.0	2.9069168134882777E-4
23	0.001958343958560524	0.0	3.824890544063523E-6	0.0	3.021663529810183E-4
24	0.0019698186301927143	0.0	3.824890544063523E-6	0.0	3.0981613406914536E-4
25	0.001996592864001159	0.0	3.824890544063523E-6	0.0	3.174659151572724E-4
26	0.0020195422072655403	3.824890544063523E-6	3.824890544063523E-6	0.0	3.4041525842165354E-4
27	0.0020883902370586837	3.824890544063523E-6	3.824890544063523E-6	0.0	3.480650395097806E-4
28	0.0021304640330433826	3.824890544063523E-6	3.824890544063523E-6	3.824890544063523E-6	3.5571482059790763E-4
29	0.002187837391204335	3.824890544063523E-6	3.824890544063523E-6	3.824890544063523E-6	3.633646016860347E-4
30	0.00221461162501278	3.824890544063523E-6	3.824890544063523E-6	3.824890544063523E-6	3.748392733182253E-4
31	0.002271984983173733	3.824890544063523E-6	3.824890544063523E-6	3.824890544063523E-6	3.824890544063523E-4
32	0.002367607246775321	3.824890544063523E-6	3.824890544063523E-6	3.824890544063523E-6	3.9396372603854287E-4
33	0.0024402801671125278	3.824890544063523E-6	3.824890544063523E-6	3.824890544063523E-6	3.977886165826064E-4
34	0.0026047504605072594	3.824890544063523E-6	3.824890544063523E-6	3.824890544063523E-6	4.0543839767073346E-4
35	0.0027309718484613554	3.824890544063523E-6	3.824890544063523E-6	3.824890544063523E-6	4.2073795984698756E-4
36	0.002807469659342626	3.824890544063523E-6	3.824890544063523E-6	3.824890544063523E-6	4.3603752202324165E-4
37	0.0029222163756645316	3.824890544063523E-6	3.824890544063523E-6	3.824890544063523E-6	4.3603752202324165E-4
38	0.0031096360123236445	7.649781088127046E-6	3.824890544063523E-6	3.824890544063523E-6	4.3603752202324165E-4
39	0.0031823089326608513	7.649781088127046E-6	3.824890544063523E-6	3.824890544063523E-6	4.436873031113687E-4
40	0.003254981852998058	7.649781088127046E-6	3.824890544063523E-6	3.824890544063523E-6	4.628117558316863E-4
41	0.003342954335511519	7.649781088127046E-6	3.824890544063523E-6	7.649781088127046E-6	4.8193620855200393E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	321860	0.0	45.839092	12
GTATCAA	320985	0.0	45.718784	9
GGTATCA	321430	0.0	45.66134	8
ACGCAGA	324905	0.0	45.651066	15
ATCAACG	323350	0.0	45.555912	11
TGGTATC	323165	0.0	45.55436	7
AAGCAGT	326655	0.0	45.53311	1
GTGGTAT	325335	0.0	45.401478	6
AGTGGTA	325035	0.0	45.34361	5
CGCAGAG	326780	0.0	45.343105	16
AGAGTAC	318435	0.0	45.299683	19
CAACGCA	326140	0.0	45.264915	13
TATCAAC	324990	0.0	45.178513	10
AACGCAG	328295	0.0	45.03293	14
CAGTGGT	329320	0.0	44.918453	4
CAGAGTA	322680	0.0	44.917133	18
GCAGAGT	325355	0.0	44.804966	17
AGCAGTG	333740	0.0	44.618595	2
GTACATG	197085	0.0	44.5592	22
GCAGTGG	334250	0.0	44.494972	3
>>END_MODULE