FastQCFastQC Report
Thu 26 May 2016
SRR805370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21434223
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2992631.396192434873893No Hit
AAGCAGTGGTATCAACGCAGAGTACATTTTTTTTTTTTTTTTTTTTTTTTTTT420520.19619092327256277No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG352990.16468523258342513No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320520.1495365612273419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1056100.043.8714141
GGTATCA1099100.042.135798
TGGTATC1101550.041.967417
GTATCAA1104150.041.7387589
TCAACGC1114400.041.64375312
ACGCAGA1113550.041.5320415
ATCAACG1116250.041.52631811
CGCAGAG1115950.041.4195516
AGAGTAC1093000.040.96697219
GCAGAGT1120600.040.38997317
CAGAGTA1121200.040.16295218
GAGTACT519750.039.8200120
CAGTGGT1167000.039.776824
AGTGGTA1173250.039.392675
GTGGTAT1184000.039.1203546
GCAGTGG1196100.039.084153
GAGTACA590950.038.67687620
AGCAGTG1202900.038.622912
TATCAAC1195300.038.59520710
AGTACAT589550.038.56144721
Produced by FastQC (version 0.11.5)