##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805370_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21434223
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.16827785173272	35.0	35.0	37.0	32.0	37.0
2	35.32999124810822	37.0	35.0	37.0	33.0	37.0
3	36.97785191466936	39.0	37.0	39.0	33.0	39.0
4	36.98695646676812	39.0	37.0	39.0	33.0	39.0
5	36.983483609366196	39.0	37.0	39.0	33.0	39.0
6	36.871888101565425	39.0	37.0	39.0	33.0	39.0
7	36.796296138189845	39.0	37.0	39.0	33.0	39.0
8	37.67043232684478	40.0	37.0	40.0	33.0	40.0
9	37.636977416909396	40.0	37.0	40.0	33.0	40.0
10	37.47078832761981	40.0	37.0	40.0	32.0	40.0
11	37.55070099811876	40.0	37.0	40.0	33.0	40.0
12	37.49143213635502	40.0	37.0	40.0	32.0	40.0
13	37.609513253641154	40.0	37.0	40.0	32.0	40.0
14	37.51959060983923	40.0	37.0	40.0	32.0	40.0
15	37.38528665116529	40.0	37.0	40.0	32.0	40.0
16	37.3446245287268	39.0	37.0	40.0	32.0	40.0
17	37.2304805263993	39.0	37.0	40.0	32.0	40.0
18	37.187551141928495	39.0	36.0	40.0	32.0	40.0
19	37.1019331561494	39.0	36.0	40.0	31.0	40.0
20	36.888951841174745	39.0	36.0	40.0	31.0	40.0
21	36.748326263098036	39.0	36.0	40.0	31.0	40.0
22	36.72358708780813	39.0	36.0	40.0	30.0	40.0
23	36.41353516756824	39.0	36.0	40.0	30.0	40.0
24	36.409954072046375	39.0	36.0	40.0	30.0	40.0
25	36.37465141610218	39.0	36.0	40.0	30.0	40.0
26	31.026042511548006	38.0	29.0	40.0	3.0	40.0
27	32.77455049338621	38.0	29.0	40.0	16.0	40.0
28	34.31362555106383	38.0	30.0	40.0	25.0	40.0
29	35.28059295641368	38.0	34.0	40.0	26.0	40.0
30	35.63038982098861	38.0	34.0	40.0	28.0	40.0
31	35.62885657203436	38.0	34.0	40.0	28.0	40.0
32	35.58311127023359	38.0	34.0	40.0	27.0	40.0
33	35.566050096614184	38.0	34.0	40.0	27.0	40.0
34	35.45674429159387	38.0	34.0	40.0	27.0	40.0
35	35.40029694568354	38.0	34.0	40.0	27.0	40.0
36	35.22659645745031	38.0	34.0	40.0	27.0	40.0
37	35.071213171571465	38.0	33.0	40.0	26.0	40.0
38	34.91695738166016	38.0	33.0	40.0	26.0	40.0
39	34.84761901562749	38.0	33.0	40.0	26.0	40.0
40	34.73258979343455	37.0	33.0	40.0	26.0	40.0
41	34.64811917838123	37.0	33.0	40.0	26.0	40.0
42	34.47887068264616	37.0	33.0	40.0	25.0	40.0
43	34.40107187463712	37.0	33.0	40.0	25.0	40.0
44	34.2506830781783	37.0	33.0	39.0	25.0	40.0
45	34.03143752866619	36.0	32.0	39.0	24.0	40.0
46	33.80023204013507	36.0	32.0	39.0	24.0	40.0
47	33.89374049154942	36.0	32.0	39.0	24.0	40.0
48	33.910560182190885	36.0	32.0	39.0	24.0	40.0
49	33.75001295824906	36.0	32.0	39.0	24.0	40.0
50	33.57910006814803	36.0	31.0	39.0	23.0	40.0
51	33.400319946284036	35.0	31.0	39.0	23.0	40.0
52	33.13780438880383	35.0	31.0	39.0	23.0	40.0
53	32.67929338982803	35.0	30.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	9.0
11	34.0
12	82.0
13	197.0
14	480.0
15	1280.0
16	2741.0
17	6049.0
18	11855.0
19	21723.0
20	35863.0
21	55554.0
22	81436.0
23	114049.0
24	153252.0
25	199515.0
26	253701.0
27	317709.0
28	393226.0
29	482792.0
30	590392.0
31	723043.0
32	889461.0
33	1117129.0
34	1431579.0
35	1888165.0
36	2532445.0
37	3362395.0
38	3727990.0
39	3040074.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.686076140945254	35.00770706733806	22.278348974908024	25.027867816808662
2	25.09339853373738	30.07757267431621	26.30027223286797	18.52875655907844
3	23.80859338824645	25.77070790016508	26.72521882412066	23.695479887467812
4	19.95402865781512	27.671056702172038	26.35565562605185	26.019259013960994
5	22.616233861148128	31.83001781776741	25.331508401307573	20.22223991977689
6	25.944910622605725	25.736169675943	26.63188210741299	21.68703759403828
7	22.27526978701304	26.68824524219982	30.595655368519775	20.440829602267367
8	25.366835084248212	25.277328690664458	27.292493877664704	22.063342347422623
9	25.516213020644603	25.13174375390235	27.784912007307195	21.56713121814586
10	21.27985698385241	26.369120074938103	31.040672666324316	21.310350274885167
11	21.12824896895026	30.59491822959946	26.714735588969095	21.56209721248118
12	21.232726747314327	25.797622801628968	30.94322103488426	22.026429416172448
13	21.47531076820466	26.376696743334243	26.018540536785494	26.1294519516756
14	21.612581897650312	30.523107835539452	26.02716226289145	21.83714800391878
15	21.64660225845369	30.47675206141132	25.84069877410532	22.03594690602967
16	21.937002335004166	26.191637550845677	25.748211166786874	26.12314894736329
17	26.522412312310085	25.85072013107263	25.879967750638784	21.746899805978504
18	21.68836724335657	25.97029526099453	25.721818794177892	26.619518701471005
19	22.17065671099904	30.408897957252755	25.58819137040797	21.832253961340236
20	26.708964444384105	25.706544156044288	25.604095842429185	21.980395557142426
21	22.193391381623677	30.3285778075557	25.51625034413424	21.961780466686385
22	26.71801072518467	25.654832461153358	25.697488544371303	21.92966826929066
23	21.97172251123822	25.52005734007713	30.632353689704544	21.87586645898011
24	21.836485511977738	30.15019018883959	25.631565930801408	22.38175836838126
25	21.928758509230775	25.602015057881967	25.711746117412325	26.75748031547493
26	20.462393248404666	34.77114612458777	26.324863747102007	18.44159687990556
27	22.249460593929623	25.527293431630344	30.21507707557209	22.008168898867947
28	24.642414143027253	25.495545138258567	28.1553989617445	21.706641756969685
29	24.353409031901926	25.633194168036788	28.22271187530334	21.790684924757944
30	24.185364685251244	25.56806467862166	28.15141001378963	22.095160622337463
31	23.451916124974534	26.02447030620144	28.164869797239678	22.35874377158435
32	23.24981409403084	26.147469866297463	28.459697372748245	22.143018666923453
33	23.153794751505572	26.166677467151477	28.31431304974293	22.365214731600023
34	22.691226082699615	26.28175511657222	28.508563151554412	22.518455649173756
35	22.614675605455815	26.603049711669048	28.598004229031304	22.184270453843837
36	22.401362531312657	26.392736513005392	28.902750521910686	22.30315043377126
37	22.38120318147292	26.384432036561346	28.821380649067613	22.412984132898124
38	22.557505350205602	26.29898457247552	28.555838016614832	22.58767206070404
39	23.016859533466643	26.093915324105755	28.349854342748976	22.539370799678625
40	22.621230543323172	26.158573604464223	28.55895919343566	22.661236658776946
41	22.71094688153613	26.23498411862189	28.665569076145193	22.38849992369679
42	22.979307437456445	26.09616872979254	28.509393599198813	22.4151302335522
43	22.615188803438315	26.66436753970508	28.406310786259898	22.31413287059671
44	22.721658723061715	26.350504984482058	28.558147407536072	22.36968888492016
45	22.877241689610113	26.37646347152402	28.575815414442594	22.17047942442327
46	22.421601193567874	26.595944252329556	28.290034119734596	22.692420434367975
47	22.519999908557452	26.400672419989284	28.4971001747999	22.582227496653363
48	22.903550084367417	26.216439009708914	28.627499116716294	22.25251178920738
49	22.610238775625316	26.63982734526929	28.324213105368923	22.42572077373647
50	22.70128942859277	26.603628225758406	28.18868684906376	22.506395496585064
51	22.719232696235363	26.577338492745923	28.33592801567848	22.367500795340238
52	22.860777365244356	26.582727071562147	28.049376923996732	22.507118639196765
53	23.09005556207939	26.5645178740559	27.806256377942884	22.53917018592183
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34998.0
1	20106.5
2	5215.0
3	4135.5
4	3056.0
5	3049.0
6	3042.0
7	3597.0
8	5089.0
9	6026.0
10	8122.5
11	10219.0
12	14076.0
13	17933.0
14	23762.5
15	29592.0
16	40157.0
17	50722.0
18	68618.0
19	86514.0
20	114137.5
21	141761.0
22	368240.0
23	594719.0
24	471865.5
25	380645.0
26	412278.0
27	452835.5
28	493393.0
29	528792.0
30	564191.0
31	593304.5
32	622418.0
33	680684.0
34	738950.0
35	786546.0
36	834142.0
37	878654.5
38	923167.0
39	966699.0
40	1010231.0
41	1029977.5
42	1090895.0
43	1132066.0
44	1193853.0
45	1255640.0
46	1277953.0
47	1300266.0
48	1439781.5
49	1579297.0
50	1593012.5
51	1606728.0
52	1535338.0
53	1463948.0
54	1320162.0
55	1176376.0
56	1080045.5
57	983715.0
58	913676.0
59	808332.0
60	773027.0
61	640107.5
62	507188.0
63	425267.5
64	343347.0
65	281528.0
66	219709.0
67	172942.5
68	126176.0
69	98112.5
70	70049.0
71	56812.5
72	43576.0
73	28795.5
74	14015.0
75	9550.5
76	3710.0
77	2334.0
78	1795.0
79	1256.0
80	998.5
81	741.0
82	556.5
83	372.0
84	250.5
85	129.0
86	89.0
87	49.0
88	36.5
89	24.0
90	16.5
91	9.0
92	6.5
93	3.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.1434223E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.047574938310746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.57000202807335	21.92926834969156
2	18.141712151705512	12.716460322923231
3	8.332431100551053	8.760945102446223
4	4.1795718116556335	5.859354251162081
5	2.185088400973281	3.8291024739972332
6	1.1880719772916275	2.49834249937412
7	0.7275966388929125	1.7850348408523662
8	0.4755585749864196	1.3333739835514251
9	0.33115955651030415	1.0445705435999373
>10	1.5891094901941551	9.845342581497327
>50	0.1546070620338719	3.856697949509428
>100	0.09120809444013692	5.306918931333869
>500	0.010935188828368005	2.8299778054739897
>1k	0.02258851656855219	15.554599590625184
>5k	2.5877325262911246E-4	0.5797698716281777
>10k+	1.0063404268909926E-4	2.270240902333998
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	299263	1.396192434873893	No Hit
AAGCAGTGGTATCAACGCAGAGTACATTTTTTTTTTTTTTTTTTTTTTTTTTT	42052	0.19619092327256277	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG	35299	0.16468523258342513	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32052	0.1495365612273419	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8661744818088344E-4	0.0	0.0	9.330872409044173E-6	9.330872409044173E-6
2	1.9128288438540551E-4	0.0	0.0	2.332718102261043E-5	9.330872409044173E-6
3	1.959483205899276E-4	0.0	0.0	5.598523445426503E-5	9.330872409044173E-6
4	1.959483205899276E-4	0.0	0.0	6.0650670658787115E-5	1.3996308613566258E-5
5	1.959483205899276E-4	0.0	0.0	6.0650670658787115E-5	1.8661744818088346E-5
6	1.959483205899276E-4	0.0	0.0	7.931241547687547E-5	1.8661744818088346E-5
7	1.959483205899276E-4	0.0	0.0	1.1197046890853006E-4	1.8661744818088346E-5
8	1.959483205899276E-4	0.0	0.0	1.1197046890853006E-4	2.332718102261043E-5
9	1.959483205899276E-4	0.0	0.0	1.1197046890853006E-4	2.332718102261043E-5
10	2.006137567944497E-4	0.0	0.0	1.2130134131757423E-4	3.26580534316546E-5
11	2.0527919299897178E-4	0.0	0.0	1.4929395854470677E-4	4.1988925840698776E-5
12	2.0527919299897178E-4	0.0	0.0	1.5395939474922884E-4	5.1319798249742946E-5
13	2.2860637402158222E-4	0.0	0.0	1.5395939474922884E-4	6.53161068633092E-5
14	2.332718102261043E-4	0.0	0.0	1.679557033627951E-4	7.931241547687547E-5
15	2.5193355504419266E-4	0.0	0.0	2.2860637402158222E-4	8.397785168139755E-5
16	2.752607360668031E-4	0.0	0.0	2.332718102261043E-4	9.330872409044172E-5
17	2.8925704468036933E-4	0.0	0.0	3.219150981120239E-4	9.330872409044172E-5
18	3.079187894984577E-4	0.0	0.0	3.312459705210681E-4	9.330872409044172E-5
19	3.079187894984577E-4	0.0	0.0	3.4057684293011225E-4	1.0263959649948589E-4
20	3.219150981120239E-4	0.0	0.0	3.4990771533915645E-4	1.0263959649948589E-4
21	3.359114067255902E-4	0.0	0.0	3.4990771533915645E-4	1.0263959649948589E-4
22	3.5457315154367855E-4	0.0	0.0	3.4990771533915645E-4	1.1663590511305215E-4
23	3.732348963617669E-4	4.665436204522086E-6	0.0	3.5923858774820065E-4	1.2130134131757423E-4
24	3.8256576877081103E-4	9.330872409044173E-6	0.0	3.5923858774820065E-4	1.306322137266184E-4
25	3.872312049753331E-4	9.330872409044173E-6	0.0	3.639040239527227E-4	1.306322137266184E-4
26	4.0589294979342147E-4	9.330872409044173E-6	0.0	3.639040239527227E-4	1.352976499311405E-4
27	4.292201308160319E-4	9.330872409044173E-6	0.0	3.639040239527227E-4	1.352976499311405E-4
28	4.5721274804316444E-4	9.330872409044173E-6	0.0	3.77900332566289E-4	1.3996308613566257E-4
29	5.131979824974295E-4	1.3996308613566258E-5	0.0	3.872312049753331E-4	1.4929395854470677E-4
30	5.551869083381283E-4	1.3996308613566258E-5	0.0	4.0589294979342147E-4	1.4929395854470677E-4
31	5.92510397974305E-4	1.3996308613566258E-5	0.0	4.1055838599794356E-4	1.4929395854470677E-4
32	6.438301962240478E-4	1.8661744818088346E-5	0.0	4.1988925840698776E-4	1.5395939474922884E-4
33	6.951499944737908E-4	1.8661744818088346E-5	0.0	4.385510032250761E-4	1.5862483095375093E-4
34	7.464697927235338E-4	1.8661744818088346E-5	0.0	4.432164394295982E-4	1.5862483095375093E-4
35	8.024550271777988E-4	1.8661744818088346E-5	0.0	4.4788187563412024E-4	1.7728657577183927E-4
36	8.817674426546743E-4	1.8661744818088346E-5	0.0	4.6187818424768654E-4	1.8195201197636134E-4
37	9.657452943360718E-4	1.8661744818088346E-5	0.0	4.758744928612528E-4	1.8195201197636134E-4
38	0.0010077342201767706	2.332718102261043E-5	0.0	5.085325462929074E-4	1.8661744818088344E-4
39	0.0010683848908355578	2.332718102261043E-5	0.0	5.085325462929074E-4	1.9128288438540551E-4
40	0.0011243701252898228	2.332718102261043E-5	0.0	5.365251635200399E-4	1.959483205899276E-4
41	0.0011756899235395657	2.332718102261043E-5	0.0	5.92510397974305E-4	1.959483205899276E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	105610	0.0	43.871414	1
GGTATCA	109910	0.0	42.13579	8
TGGTATC	110155	0.0	41.96741	7
GTATCAA	110415	0.0	41.738758	9
TCAACGC	111440	0.0	41.643753	12
ACGCAGA	111355	0.0	41.53204	15
ATCAACG	111625	0.0	41.526318	11
CGCAGAG	111595	0.0	41.41955	16
AGAGTAC	109300	0.0	40.966972	19
GCAGAGT	112060	0.0	40.389973	17
CAGAGTA	112120	0.0	40.162952	18
GAGTACT	51975	0.0	39.82001	20
CAGTGGT	116700	0.0	39.77682	4
AGTGGTA	117325	0.0	39.39267	5
GTGGTAT	118400	0.0	39.120354	6
GCAGTGG	119610	0.0	39.08415	3
GAGTACA	59095	0.0	38.676876	20
AGCAGTG	120290	0.0	38.62291	2
TATCAAC	119530	0.0	38.595207	10
AGTACAT	58955	0.0	38.561447	21
>>END_MODULE