FastQCFastQC Report
Thu 26 May 2016
SRR805369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18078800
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2188311.2104287895214285No Hit
AAGCAGTGGTATCAACGCAGAGTACATTTTTTTTTTTTTTTTTTTTTTTTTTT396450.2192899971237029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC948000.043.24198512
AAGCAGT949100.043.2116741
GGTATCA954550.043.0166558
ACGCAGA952150.042.83138315
TGGTATC958200.042.816017
GTATCAA956150.042.6890689
ATCAACG960750.042.6216511
AGAGTAC932900.042.3599519
CGCAGAG965500.042.23671716
GTGGTAT982900.041.773536
AGTGGTA985650.041.5663765
CAGAGTA962000.041.3326418
GCAGAGT968400.041.23177317
TATCAAC995800.041.05302410
CAGTGGT1002500.040.987284
CAACGCA1000850.040.9609313
AACGCAG1008400.040.59599314
GAGTACA580400.040.363820
GTACATG506250.040.2969922
GCAGTGG1028100.040.2318343
Produced by FastQC (version 0.11.5)