##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805369_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18078800
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.62938369803305	35.0	35.0	37.0	32.0	37.0
2	34.88986769033343	35.0	35.0	37.0	32.0	37.0
3	36.615029260791644	39.0	35.0	39.0	32.0	39.0
4	36.693932174701864	39.0	35.0	39.0	32.0	39.0
5	36.698566497776405	39.0	37.0	39.0	32.0	39.0
6	36.658111710954266	39.0	35.0	39.0	32.0	39.0
7	36.563401055379785	39.0	35.0	39.0	32.0	39.0
8	37.42360079208797	40.0	37.0	40.0	32.0	40.0
9	37.388846992057	40.0	37.0	40.0	32.0	40.0
10	37.19987255791314	39.0	36.0	40.0	32.0	40.0
11	37.27904672876518	39.0	36.0	40.0	32.0	40.0
12	37.19928474235016	39.0	36.0	40.0	32.0	40.0
13	37.336643029404605	40.0	37.0	40.0	32.0	40.0
14	37.21896547337213	40.0	37.0	40.0	32.0	40.0
15	37.064094906741595	39.0	36.0	40.0	31.0	40.0
16	37.04227714228821	39.0	36.0	40.0	31.0	40.0
17	36.932612949974555	39.0	36.0	40.0	31.0	40.0
18	36.88663108170896	39.0	36.0	40.0	31.0	40.0
19	36.78632541982875	39.0	36.0	40.0	31.0	40.0
20	36.54471225966325	39.0	36.0	40.0	30.0	40.0
21	36.34974616678098	39.0	36.0	40.0	30.0	40.0
22	36.343338053410626	39.0	36.0	40.0	30.0	40.0
23	36.01933043122331	38.0	35.0	40.0	29.0	40.0
24	36.01161426643361	38.0	35.0	40.0	29.0	40.0
25	35.9892025466292	38.0	35.0	40.0	29.0	40.0
26	30.135379505276898	37.0	25.0	40.0	3.0	40.0
27	32.162298437949424	37.0	25.0	40.0	16.0	40.0
28	33.84116512157887	37.0	30.0	40.0	24.0	40.0
29	34.85307802509016	37.0	33.0	40.0	25.0	40.0
30	35.2141189680731	38.0	34.0	40.0	26.0	40.0
31	35.21905065601699	38.0	34.0	40.0	26.0	40.0
32	35.19090326791601	38.0	34.0	40.0	26.0	40.0
33	35.1996128061597	38.0	34.0	40.0	26.0	40.0
34	35.096439752638446	38.0	33.0	40.0	26.0	40.0
35	35.04357534792132	38.0	33.0	40.0	26.0	40.0
36	34.90802121822245	38.0	33.0	40.0	25.0	40.0
37	34.736814722216074	38.0	33.0	40.0	25.0	40.0
38	34.554676859083564	37.0	33.0	40.0	24.0	40.0
39	34.475501858530436	37.0	33.0	40.0	24.0	40.0
40	34.362359614576185	37.0	33.0	40.0	24.0	40.0
41	34.3097444520654	37.0	33.0	40.0	24.0	40.0
42	34.154259021616475	37.0	32.0	40.0	24.0	40.0
43	34.10091173086709	37.0	32.0	40.0	24.0	40.0
44	33.93811597008651	37.0	32.0	39.0	24.0	40.0
45	33.73602827621302	36.0	32.0	39.0	23.0	40.0
46	33.53102871872027	36.0	31.0	39.0	23.0	40.0
47	33.62383991194106	36.0	31.0	39.0	23.0	40.0
48	33.67549201274421	36.0	31.0	39.0	23.0	40.0
49	33.51510996305065	36.0	31.0	39.0	23.0	40.0
50	33.34773607761577	36.0	31.0	39.0	22.0	40.0
51	33.18635562094829	36.0	31.0	39.0	22.0	40.0
52	32.91409512799522	35.0	31.0	39.0	21.0	40.0
53	32.46680034073058	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	20.0
11	59.0
12	143.0
13	350.0
14	864.0
15	2112.0
16	4461.0
17	8753.0
18	16092.0
19	27442.0
20	43843.0
21	65632.0
22	92405.0
23	125509.0
24	164924.0
25	209991.0
26	263130.0
27	320052.0
28	388337.0
29	465859.0
30	555956.0
31	664774.0
32	798220.0
33	974071.0
34	1214740.0
35	1553012.0
36	2042203.0
37	2678720.0
38	2963809.0
39	2433310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.292027125694183	37.90650375024891	16.255592185322033	20.54587693873487
2	27.276732969002364	30.884411575989557	25.916626103502445	15.922229351505631
3	25.695997521959423	24.592622297940128	26.118813195566077	23.592566984534372
4	21.313732106113235	28.554760271699447	24.171217116180276	25.960290506007034
5	21.770471491470673	32.125561431068434	23.44605283536518	22.657914242095714
6	26.266494457596746	24.43621811182158	27.29447750956922	22.002809921012457
7	21.54535146138018	25.690034736818816	29.661437706042438	23.103176095758567
8	25.668197004225945	24.398488837754716	26.711938845498594	23.221375312520742
9	26.665796402416092	25.04703852025577	26.02375710777264	22.2634079695555
10	21.660779476513927	25.940410867977963	30.716800893864637	21.682008761643473
11	21.652095271810076	30.67659911056044	25.990165276456402	21.681140341173087
12	21.80344381264243	24.950123902028896	30.773806889837825	22.47262539549085
13	22.108314711153394	25.586283381640374	25.359913268579774	26.945488638626458
14	22.06293559307034	30.290837887470406	25.473720600924842	22.172505918534416
15	22.1173971723787	29.918362944443217	25.32300816425869	22.641231718919396
16	22.54602075358984	25.35709781622674	25.061552757926407	27.03532867225701
17	27.228239706175188	25.240497156890946	25.249917029891368	22.281346107042502
18	22.19889594442109	25.090503794499636	24.93574241653207	27.774857844547206
19	22.814755404119744	30.0153660641193	24.722503705998186	22.447374825762772
20	27.387005774719558	25.07303582096157	25.02813239816802	22.51182600615085
21	22.732122707259332	29.63228200986791	24.88464942363431	22.750945859238445
22	27.670426134477953	24.90265393720822	24.959057017058655	22.46786291125517
23	22.38192247273049	24.983455760338074	30.160928822709472	22.473692944221963
24	22.441876673230524	29.639555722724957	24.889434033232295	23.02913357081222
25	22.608032612784037	24.879372524725092	24.956634289886498	27.555960572604377
26	20.108137708254972	37.07019271190566	24.41534836382946	18.406321216009914
27	22.86259596875899	24.621683961324866	29.8242803725911	22.691439697325045
28	25.699133792065844	24.90742195278448	27.095316060800496	22.298128194349182
29	25.350620616412595	25.151802110759565	27.172245945527358	22.325331327300486
30	25.243428767396065	24.749955749275397	27.23866075181981	22.767954731508727
31	24.519912826072527	25.313964422417417	27.09120627475275	23.074916476757306
32	23.8159944244087	25.76174303604222	27.48496028497467	22.93730225457442
33	23.717807597849415	25.373719494656726	27.603983671482617	23.30448923601124
34	23.670901829767462	25.60286080934575	27.551701440361086	23.1745359205257
35	23.3362999756621	26.000990109963052	27.722741553642944	22.939968360731907
36	23.31192888908556	25.52016726773901	27.81898687966016	23.34891696351528
37	23.61212027346948	25.564843905568953	27.59205256986083	23.23098325110074
38	23.542264973338938	25.59079142420957	27.66029824988384	23.206645352567648
39	23.73823483859548	25.273696263026306	27.471795694404495	23.516273203973714
40	23.56456733854017	25.493898931345	27.486044427727503	23.455489302387328
41	23.433662632475606	25.75679248622696	27.6122751510056	23.197269730291833
42	23.774470650706906	25.405696174524856	27.52707591211806	23.292757262650177
43	23.721485939332258	25.80531340575702	27.293260614642566	23.17994004026816
44	23.61459278270682	25.66888842179791	27.631170210412197	23.085348585083082
45	23.723172998207843	25.39951213576122	27.596029603734763	23.28128526229617
46	23.526683186937188	25.630639201716924	27.27174923114366	23.570928380202226
47	23.37229794012877	25.62402371838839	27.614576188685092	23.389102152797754
48	23.69828749695776	25.326454189437353	27.643853574352278	23.331404739252605
49	23.581017545412305	25.827068168241258	27.262069385136183	23.329844901210254
50	23.577516206827887	25.798366041993937	27.490121025731796	23.13399672544638
51	23.596383609531607	25.53628006283603	27.46816713498684	23.39916919264553
52	23.83813084939266	25.70807243843618	27.00877823749364	23.44501847467752
53	23.733466823019228	25.853552226917714	27.073024758280418	23.33995619178264
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12190.0
1	8118.0
2	4046.0
3	3593.5
4	3141.0
5	3336.5
6	3532.0
7	3941.0
8	5119.5
9	5889.0
10	7210.5
11	8532.0
12	11263.0
13	13994.0
14	17693.0
15	21392.0
16	28062.5
17	34733.0
18	45663.5
19	56594.0
20	74440.0
21	92286.0
22	258155.0
23	424024.0
24	329517.0
25	246645.0
26	258280.0
27	282527.5
28	306775.0
29	331945.0
30	357115.0
31	383295.5
32	409476.0
33	454437.0
34	499398.0
35	543249.0
36	587100.0
37	635612.0
38	684124.0
39	735112.0
40	786100.0
41	835447.0
42	941973.5
43	999153.0
44	1062502.0
45	1125851.0
46	1165972.5
47	1206094.0
48	1274910.5
49	1343727.0
50	1357060.0
51	1370393.0
52	1338091.0
53	1305789.0
54	1230032.0
55	1154275.0
56	1081431.0
57	1008587.0
58	925844.0
59	767173.0
60	691245.0
61	593105.0
62	494965.0
63	418812.0
64	342659.0
65	281584.0
66	220509.0
67	175269.0
68	130029.0
69	102403.0
70	74777.0
71	57125.0
72	39473.0
73	28885.0
74	18297.0
75	13267.5
76	6127.0
77	4016.0
78	3179.5
79	2343.0
80	1835.0
81	1327.0
82	987.5
83	648.0
84	440.0
85	232.0
86	167.0
87	102.0
88	74.0
89	46.0
90	38.0
91	30.0
92	21.0
93	7.5
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.80788E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.12949704215113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.46283948286055	33.680076820311974
2	15.374983041459334	14.492304355511628
3	5.417991913427703	7.660417015748691
4	2.4549143164044605	4.627955080548739
5	1.315070874223703	3.0989314438472553
6	0.8486648795846528	2.399828935929746
7	0.5318044129985928	1.754457215659407
8	0.3999350758427548	1.507899117918688
9	0.3127676812747707	1.32665251585678
>10	1.7410929594577327	14.602655487718295
>50	0.07846186397755545	2.4797887010001136
>100	0.04905386473219548	5.2304803145722385
>500	0.009196274934561556	2.9727719335877003
>1k	0.0030757828455196925	2.1580635853879615
>5k	9.838398408156484E-5	0.3579600094184243
>10k+	4.919199204078242E-5	1.6497574669825366
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	218831	1.2104287895214285	No Hit
AAGCAGTGGTATCAACGCAGAGTACATTTTTTTTTTTTTTTTTTTTTTTTTTT	39645	0.2192899971237029	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.425072460561542E-5	0.0	0.0	5.531340575701927E-6	1.1062681151403854E-5
2	5.531340575701927E-5	0.0	0.0	1.1062681151403854E-5	1.6594021727105782E-5
3	5.531340575701927E-5	0.0	0.0	1.6594021727105782E-5	2.7656702878509635E-5
4	5.531340575701927E-5	0.0	0.0	1.6594021727105782E-5	2.7656702878509635E-5
5	5.531340575701927E-5	0.0	0.0	2.212536230280771E-5	4.425072460561542E-5
6	6.08447463327212E-5	0.0	0.0	2.212536230280771E-5	5.531340575701927E-5
7	6.08447463327212E-5	0.0	0.0	2.7656702878509635E-5	6.08447463327212E-5
8	6.08447463327212E-5	1.6594021727105782E-5	0.0	2.7656702878509635E-5	6.637608690842313E-5
9	6.08447463327212E-5	1.6594021727105782E-5	0.0	2.7656702878509635E-5	7.190742748412506E-5
10	6.08447463327212E-5	1.6594021727105782E-5	0.0	3.3188043454211564E-5	8.29701086355289E-5
11	6.08447463327212E-5	1.6594021727105782E-5	0.0	3.871938402991349E-5	8.850144921123083E-5
12	6.08447463327212E-5	1.6594021727105782E-5	0.0	4.425072460561542E-5	1.1615815208974047E-4
13	9.956413036263469E-5	1.6594021727105782E-5	0.0	5.531340575701927E-5	1.216894926654424E-4
14	1.1062681151403854E-4	1.6594021727105782E-5	0.0	5.531340575701927E-5	1.2722083324114431E-4
15	1.3275217381684626E-4	1.6594021727105782E-5	0.0	1.0509547093833661E-4	1.3275217381684626E-4
16	1.4381485496825012E-4	1.6594021727105782E-5	0.0	1.1062681151403854E-4	1.4934619554395203E-4
17	1.4381485496825012E-4	1.6594021727105782E-5	0.0	1.4934619554395203E-4	1.604088766953559E-4
18	1.4934619554395203E-4	1.6594021727105782E-5	0.0	1.604088766953559E-4	1.604088766953559E-4
19	1.659402172710578E-4	1.6594021727105782E-5	0.0	1.659402172710578E-4	1.8253423899816358E-4
20	1.7700289842246167E-4	1.6594021727105782E-5	0.0	1.659402172710578E-4	1.8253423899816358E-4
21	1.8806557957386553E-4	1.6594021727105782E-5	0.0	1.659402172710578E-4	1.8806557957386553E-4
22	1.9912826072526939E-4	2.7656702878509635E-5	0.0	1.8253423899816358E-4	2.046596013009713E-4
23	2.2125362302807708E-4	3.3188043454211564E-5	0.0	1.8806557957386553E-4	2.1019094187667322E-4
24	2.4891032590658674E-4	3.3188043454211564E-5	0.0	1.8806557957386553E-4	2.1572228245237516E-4
25	2.5997300705799057E-4	3.3188043454211564E-5	5.531340575701927E-6	2.046596013009713E-4	2.2125362302807708E-4
26	2.8762970993650023E-4	3.3188043454211564E-5	5.531340575701927E-6	2.1019094187667322E-4	2.3784764475518288E-4
27	3.1528641281500984E-4	3.3188043454211564E-5	5.531340575701927E-6	2.1019094187667322E-4	2.4891032590658674E-4
28	3.5400579684492333E-4	3.3188043454211564E-5	5.531340575701927E-6	2.1019094187667322E-4	2.5997300705799057E-4
29	4.1485054317764455E-4	3.3188043454211564E-5	5.531340575701927E-6	2.1572228245237516E-4	2.655043476336925E-4
30	4.480385866318561E-4	3.3188043454211564E-5	5.531340575701927E-6	2.2125362302807708E-4	2.8762970993650023E-4
31	5.420713764187888E-4	3.3188043454211564E-5	5.531340575701927E-6	2.3231630417948094E-4	3.04223731663606E-4
32	6.305728256300197E-4	3.3188043454211564E-5	5.531340575701927E-6	2.4891032590658674E-4	3.208177533907118E-4
33	7.743876805982698E-4	3.3188043454211564E-5	5.531340575701927E-6	2.5444166648228863E-4	3.263490939664137E-4
34	9.513905790207315E-4	3.3188043454211564E-5	5.531340575701927E-6	2.5997300705799057E-4	3.484744562692214E-4
35	0.0010288293470805585	3.3188043454211564E-5	5.531340575701927E-6	2.655043476336925E-4	3.5400579684492333E-4
36	0.001150518839746001	3.3188043454211564E-5	5.531340575701927E-6	2.710356882093944E-4	3.7613115914773105E-4
37	0.001211363586078722	3.3188043454211564E-5	5.531340575701927E-6	2.7656702878509635E-4	3.7613115914773105E-4
38	0.0012666769918357414	3.3188043454211564E-5	5.531340575701927E-6	2.9869239108790406E-4	3.81662499723433E-4
39	0.0013828351439254818	3.871938402991349E-5	5.531340575701927E-6	2.9869239108790406E-4	4.0378786202624066E-4
40	0.001515587317742328	3.871938402991349E-5	5.531340575701927E-6	2.9869239108790406E-4	4.093192026019426E-4
41	0.001581963404650751	4.425072460561542E-5	5.531340575701927E-6	2.9869239108790406E-4	4.2038188375334644E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	94800	0.0	43.241985	12
AAGCAGT	94910	0.0	43.211674	1
GGTATCA	95455	0.0	43.016655	8
ACGCAGA	95215	0.0	42.831383	15
TGGTATC	95820	0.0	42.81601	7
GTATCAA	95615	0.0	42.689068	9
ATCAACG	96075	0.0	42.62165	11
AGAGTAC	93290	0.0	42.35995	19
CGCAGAG	96550	0.0	42.236717	16
GTGGTAT	98290	0.0	41.77353	6
AGTGGTA	98565	0.0	41.566376	5
CAGAGTA	96200	0.0	41.33264	18
GCAGAGT	96840	0.0	41.231773	17
TATCAAC	99580	0.0	41.053024	10
CAGTGGT	100250	0.0	40.98728	4
CAACGCA	100085	0.0	40.96093	13
AACGCAG	100840	0.0	40.595993	14
GAGTACA	58040	0.0	40.3638	20
GTACATG	50625	0.0	40.29699	22
GCAGTGG	102810	0.0	40.231834	3
>>END_MODULE