FastQCFastQC Report
Thu 26 May 2016
SRR805368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17375178
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT974500.5608575635887011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC543000.041.20506712
AAGCAGT547550.040.8884121
GGTATCA546300.040.7367748
GTATCAA546400.040.7078139
ACGCAGA550900.040.47340815
ATCAACG552550.040.46312711
TGGTATC552150.040.3009157
AGTGGTA563350.039.4579735
GTGGTAT565850.039.333486
CGCAGAG567900.039.2411516
AGAGTAC552850.039.13620419
TATCAAC574400.038.80936410
CAACGCA577750.038.78771213
CAGTGGT582850.038.3192944
AACGCAG585900.038.2120714
CAGAGTA578700.037.566718
GCAGAGT581200.037.53854417
AGCAGTG601400.037.2975582
GCAGTGG610250.036.9145433
GAGTACA382000.036.03128420
Produced by FastQC (version 0.11.5)