##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805368_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17375178
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.680022731277916	35.0	35.0	37.0	32.0	37.0
2	34.93384493672525	35.0	35.0	37.0	32.0	37.0
3	36.68019487339928	39.0	35.0	39.0	32.0	39.0
4	36.76231685223599	39.0	37.0	39.0	32.0	39.0
5	36.77169183532969	39.0	37.0	39.0	32.0	39.0
6	36.706029141111536	39.0	37.0	39.0	32.0	39.0
7	36.637224781236775	39.0	35.0	39.0	32.0	39.0
8	37.48351694584078	40.0	37.0	40.0	32.0	40.0
9	37.42880562144457	40.0	37.0	40.0	32.0	40.0
10	37.32551476594945	39.0	37.0	40.0	32.0	40.0
11	37.3678579292828	40.0	37.0	40.0	32.0	40.0
12	37.27699756514725	39.0	36.0	40.0	32.0	40.0
13	37.3959078865264	40.0	37.0	40.0	32.0	40.0
14	37.261722786379515	40.0	37.0	40.0	32.0	40.0
15	37.127451701502	39.0	36.0	40.0	32.0	40.0
16	37.093112887821924	39.0	36.0	40.0	32.0	40.0
17	36.972770235792694	39.0	36.0	40.0	31.0	40.0
18	36.95429871279592	39.0	36.0	40.0	31.0	40.0
19	36.84136778339767	39.0	36.0	40.0	31.0	40.0
20	36.60359680919528	39.0	36.0	40.0	30.0	40.0
21	36.457832662203515	39.0	36.0	40.0	30.0	40.0
22	36.41746334915245	39.0	36.0	40.0	30.0	40.0
23	36.17573540829337	39.0	35.0	40.0	30.0	40.0
24	36.14810697191131	39.0	35.0	40.0	30.0	40.0
25	36.06988365817029	38.0	35.0	40.0	29.0	40.0
26	29.83396860740074	37.0	24.0	40.0	3.0	40.0
27	31.919528133754945	37.0	25.0	40.0	16.0	40.0
28	33.72997721231978	37.0	30.0	40.0	24.0	40.0
29	34.79884430536482	37.0	33.0	40.0	25.0	40.0
30	35.19195843633947	38.0	34.0	40.0	26.0	40.0
31	35.23582820273841	38.0	34.0	40.0	26.0	40.0
32	35.213874873684745	38.0	34.0	40.0	26.0	40.0
33	35.219105208591245	38.0	34.0	40.0	26.0	40.0
34	35.11428964929165	38.0	33.0	40.0	26.0	40.0
35	35.056326329433865	38.0	33.0	40.0	26.0	40.0
36	34.92683787181921	38.0	33.0	40.0	25.0	40.0
37	34.75593061550218	38.0	33.0	40.0	25.0	40.0
38	34.61640312404282	38.0	33.0	40.0	25.0	40.0
39	34.55781863069259	37.0	33.0	40.0	24.0	40.0
40	34.445237280446854	37.0	33.0	40.0	24.0	40.0
41	34.39412850907196	37.0	33.0	40.0	24.0	40.0
42	34.246540438319535	37.0	33.0	40.0	24.0	40.0
43	34.1831344116302	37.0	32.0	40.0	24.0	40.0
44	34.011746872463696	37.0	32.0	39.0	24.0	40.0
45	33.79223902051536	37.0	32.0	39.0	23.0	40.0
46	33.588155816303	36.0	31.0	39.0	23.0	40.0
47	33.71096307617683	37.0	31.0	39.0	23.0	40.0
48	33.76397531006589	37.0	31.0	39.0	23.0	40.0
49	33.603275891619646	36.0	31.0	39.0	23.0	40.0
50	33.454842649669544	36.0	31.0	39.0	23.0	40.0
51	33.3139140790385	36.0	31.0	39.0	22.0	40.0
52	33.06497124806434	36.0	31.0	39.0	22.0	40.0
53	32.62947153692468	35.0	30.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	17.0
11	58.0
12	146.0
13	365.0
14	970.0
15	2083.0
16	4377.0
17	8148.0
18	14783.0
19	25226.0
20	39999.0
21	59145.0
22	83924.0
23	113721.0
24	148943.0
25	192252.0
26	241522.0
27	295783.0
28	361604.0
29	436545.0
30	524867.0
31	632318.0
32	764911.0
33	937584.0
34	1176640.0
35	1515100.0
36	2002347.0
37	2620264.0
38	2905919.0
39	2265613.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.379996452410445	30.411147442633396	22.98436885078242	23.22448725417374
2	26.20442794888202	26.498255154565896	26.683007218688637	20.614309677863442
3	24.756517602294494	24.619413970895724	25.626759046727464	24.99730938008232
4	22.776911983290184	27.496155722836335	24.642953298089953	25.083978995783525
5	24.729145220843204	29.17296156620669	23.775860022844082	22.322033190106026
6	26.223449336749237	24.846801569457302	25.828564173558394	23.101184920235063
7	23.531390584890698	25.68543470461137	27.860612420776356	22.92256228972158
8	25.09764216516228	24.903537678865796	26.20652864678566	23.792291509186267
9	25.42619131729183	24.914939000912682	26.32508858326516	23.33378109853033
10	22.93140248692704	25.633348907274506	28.464905510608297	22.970343095190163
11	22.78962552210976	28.22882159825931	25.94651979968205	23.03503307994888
12	22.833970391555127	25.20228569744724	28.459530026109658	23.50421388488797
13	23.09012891839151	25.567162534967984	25.502904200463444	25.839804346177058
14	23.096154755939764	28.086123779566456	25.565499242655243	23.252222221838533
15	23.153794453213656	27.842563684815204	25.43130781163796	23.572334050333183
16	23.423817586214078	25.392810364302456	25.320085929479397	25.863286120004066
17	26.05451869327612	25.24695862108578	25.430645947914893	23.267876737723206
18	23.27951978391243	25.134154021328587	25.26008078881264	26.326245405946345
19	23.616270290871263	27.84525718240124	25.117503832190955	23.42096869453654
20	26.13513369474546	25.167747921776684	25.333179320522643	23.36393906295521
21	23.596949625494485	27.623820602010525	25.1794139893128	23.599815783182194
22	26.304910372716755	25.049187985297188	25.222084055771976	23.423817586214078
23	23.432830443521212	25.045567878498858	28.12002271286084	23.40157896511909
24	23.4649509777684	27.604425117256355	25.194654120953462	23.735969784021783
25	23.521548959095558	25.036814011344227	25.168674530988977	26.27296249857124
26	21.5699257872351	35.45863530146281	24.26188094303264	18.709557968269447
27	23.674899906061395	24.84227787479357	27.84141261747074	23.641409601674297
28	25.66528527074658	24.940676866734833	26.162085936616013	23.23195192590257
29	25.36489698119927	25.13573673892722	26.159651429182478	23.339714850691028
30	25.28879991905694	24.91514043769796	26.124025894871405	23.672033748373686
31	24.92372164475092	25.24985931079382	26.042858381076726	23.783560663378527
32	24.426644722718812	25.529315440682105	26.22811115949431	23.815928677104775
33	24.329385287448567	25.30063864669473	26.311972170875027	24.05800389498168
34	24.28025197785024	25.425610028282875	26.33392878047062	23.96020921339626
35	24.13822177821718	25.617199432431715	26.427021351953922	23.817557437397188
36	24.09906246715861	25.384119805851775	26.459867058628117	24.056950668361498
37	24.320844367752663	25.446041473647064	26.2916846089289	23.941429549671376
38	24.262761509551154	25.498604963931882	26.335712934854538	23.902920591662426
39	24.403473737074812	25.243798941225236	26.25117279374059	24.10155452795937
40	24.267630524418223	25.456683091246607	26.1943848863016	24.081301498033575
41	24.2130526662806	25.631052527922304	26.286660199970324	23.869234605826772
42	24.36239214355099	25.39252259746634	26.231644936241803	24.013440322740866
43	24.40008384374537	25.57923147607466	26.088003242326497	23.93268143785347
44	24.374432307974054	25.428758197469975	26.308225446668803	23.888584047887164
45	24.442184131869038	25.230394761998987	26.256761225697943	24.070659880434032
46	24.32462562397922	25.458409692263295	25.9932070911734	24.22375759258409
47	24.161266146453293	25.421017269578478	26.360443616750285	24.057272967217948
48	24.332780936114727	25.242883842686386	26.288709099843466	24.13562612135542
49	24.275918209298343	25.64229270054097	26.042530326883558	24.03925876327713
50	24.319791141132484	25.56781864335433	26.213544402250154	23.898845813263037
51	24.335129113497427	25.332114583229014	26.219155855554398	24.113600447719154
52	24.48307579928102	25.50324952066678	25.955014676684172	24.058660003368022
53	24.438299279581482	25.585648676520034	25.977840342124843	23.998211701773645
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8864.0
1	5778.0
2	2692.0
3	2495.0
4	2298.0
5	2505.5
6	2713.0
7	3030.5
8	3908.5
9	4469.0
10	5472.0
11	6475.0
12	8276.5
13	10078.0
14	12677.5
15	15277.0
16	20090.0
17	24903.0
18	32936.0
19	40969.0
20	54578.0
21	68187.0
22	147321.0
23	226455.0
24	194021.0
25	176739.0
26	191891.0
27	215518.5
28	239146.0
29	264417.0
30	289688.0
31	314839.0
32	339990.0
33	380729.5
34	421469.0
35	467984.0
36	514499.0
37	566566.5
38	618634.0
39	672826.0
40	727018.0
41	782039.0
42	895566.0
43	954072.0
44	1020477.0
45	1086882.0
46	1136071.5
47	1185261.0
48	1247311.0
49	1309361.0
50	1334666.5
51	1359972.0
52	1342606.0
53	1325240.0
54	1267632.0
55	1210024.0
56	1138092.5
57	1066161.0
58	984203.0
59	818323.5
60	734402.0
61	635455.0
62	536508.0
63	455161.0
64	373814.0
65	308563.5
66	243313.0
67	196688.0
68	150063.0
69	118935.5
70	87808.0
71	67475.5
72	47143.0
73	34723.0
74	22303.0
75	16832.0
76	8460.0
77	5559.0
78	4198.0
79	2837.0
80	2244.5
81	1652.0
82	1213.0
83	774.0
84	568.0
85	362.0
86	268.5
87	175.0
88	132.5
89	90.0
90	69.5
91	49.0
92	36.5
93	16.0
94	8.0
95	6.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.7375178E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.55445405965702
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.266882491739	41.132732803046714
2	11.539044328072038	12.128563500640157
3	3.76096638714659	5.929666056396291
4	1.840471257807319	3.868998486662157
5	1.0050939128714809	2.6411080934822584
6	0.667207249756601	2.1038827653362038
7	0.4710003608591066	1.732721677879626
8	0.3406781999901944	1.4323325448409057
9	0.2632869990562476	1.245321404676587
>10	1.7103953461072026	16.304603035136694
>50	0.07874851459690102	2.7674746861409414
>100	0.05120402755334547	6.079856332571203
>500	0.004551750447608555	1.5341750704865134
>1k	4.0218611218359636E-4	0.32420352814065917
>5k	5.582323682368627E-5	0.2025702421899978
>10k+	1.1164647364737249E-5	0.5717897723732845
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97450	0.5608575635887011	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0016978243330802137	0.0	0.0	0.0	2.3021346889223237E-5
2	0.0018762397714716936	0.0	0.0	0.0	2.8776683611529045E-5
3	0.0018762397714716936	0.0	0.0	0.0	4.604269377844647E-5
4	0.0019050164550832227	0.0	0.0	0.0	6.906404066766971E-5
5	0.0019107717918055286	0.0	0.0	0.0	8.057471411228133E-5
6	0.001985591169195504	0.0	0.0	0.0	9.784072427919875E-5
7	0.001985591169195504	0.0	0.0	5.755336722305809E-6	9.784072427919875E-5
8	0.001985591169195504	0.0	0.0	5.755336722305809E-6	1.0935139772381036E-4
9	0.001985591169195504	0.0	0.0	1.726601016691743E-5	1.266174078907278E-4
10	0.001985591169195504	0.0	0.0	1.726601016691743E-5	1.4388341805764523E-4
11	0.00199134650591781	0.0	0.0	1.726601016691743E-5	1.5539409150225683E-4
12	0.00199134650591781	0.0	0.0	1.726601016691743E-5	1.7266010166917426E-4
13	0.002077676556752397	0.0	5.755336722305809E-6	1.726601016691743E-5	1.956814485583975E-4
14	0.002083431893474703	0.0	5.755336722305809E-6	1.726601016691743E-5	2.1294745872531493E-4
15	0.002140985260697761	0.0	5.755336722305809E-6	3.453202033383486E-5	2.2445813216992656E-4
16	0.0021582512708646783	0.0	5.755336722305809E-6	3.453202033383486E-5	2.3596880561453816E-4
17	0.002164006607586984	0.0	1.1510673444611618E-5	6.33087039453639E-5	2.70500825948373E-4
18	0.002198538627920819	0.0	1.1510673444611618E-5	7.481937738997551E-5	2.9352217283759623E-4
19	0.002227315311532348	0.0	1.1510673444611618E-5	7.481937738997551E-5	2.9352217283759623E-4
20	0.0022388259849769596	5.755336722305809E-6	1.1510673444611618E-5	7.481937738997551E-5	3.165435197268195E-4
21	0.0022848686787554063	5.755336722305809E-6	1.1510673444611618E-5	7.481937738997551E-5	3.222988564491253E-4
22	0.002319400699089241	5.755336722305809E-6	1.1510673444611618E-5	7.481937738997551E-5	3.338095298937369E-4
23	0.0023654433928676877	1.1510673444611618E-5	1.1510673444611618E-5	8.057471411228133E-5	3.338095298937369E-4
24	0.0023999754132015224	1.1510673444611618E-5	1.1510673444611618E-5	8.057471411228133E-5	3.453202033383485E-4
25	0.0024460181069799686	1.1510673444611618E-5	1.1510673444611618E-5	8.633005083458713E-5	3.683415502275718E-4
26	0.0024920608007584152	1.1510673444611618E-5	1.1510673444611618E-5	9.208538755689295E-5	3.91362897116795E-4
27	0.002538103494536862	1.1510673444611618E-5	1.1510673444611618E-5	9.208538755689295E-5	4.028735705614066E-4
28	0.0026244335453714488	1.1510673444611618E-5	1.1510673444611618E-5	9.208538755689295E-5	4.316502541729357E-4
29	0.0027568062899844823	1.1510673444611618E-5	1.1510673444611618E-5	9.208538755689295E-5	4.316502541729357E-4
30	0.002797093647040623	1.726601016691743E-5	1.1510673444611618E-5	9.208538755689295E-5	4.431609276175473E-4
31	0.002923711054931351	1.726601016691743E-5	1.1510673444611618E-5	9.208538755689295E-5	4.661822745067705E-4
32	0.0029639984119874917	1.726601016691743E-5	1.1510673444611618E-5	9.208538755689295E-5	4.7769294795138215E-4
33	0.003015796442488244	1.726601016691743E-5	1.1510673444611618E-5	9.784072427919875E-5	5.12224968285217E-4
34	0.003102126493322831	1.726601016691743E-5	1.1510673444611618E-5	9.784072427919875E-5	5.237356417298286E-4
35	0.0032402545746581706	1.726601016691743E-5	1.1510673444611618E-5	1.0359606100150456E-4	5.352463151744402E-4
36	0.0033496059723819808	1.726601016691743E-5	1.1510673444611618E-5	1.0359606100150456E-4	5.640229987859693E-4
37	0.0035452874209403783	1.726601016691743E-5	1.1510673444611618E-5	1.0359606100150456E-4	5.812890089528867E-4
38	0.003671904828831106	1.726601016691743E-5	1.1510673444611618E-5	1.0359606100150456E-4	5.870443456751925E-4
39	0.003798522236721834	1.726601016691743E-5	1.1510673444611618E-5	1.0359606100150456E-4	6.100656925644158E-4
40	0.0039193843078902555	1.726601016691743E-5	1.1510673444611618E-5	1.0359606100150456E-4	6.273317027313332E-4
41	0.004069023062670207	1.726601016691743E-5	1.1510673444611618E-5	1.0359606100150456E-4	6.33087039453639E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	54300	0.0	41.205067	12
AAGCAGT	54755	0.0	40.888412	1
GGTATCA	54630	0.0	40.736774	8
GTATCAA	54640	0.0	40.707813	9
ACGCAGA	55090	0.0	40.473408	15
ATCAACG	55255	0.0	40.463127	11
TGGTATC	55215	0.0	40.300915	7
AGTGGTA	56335	0.0	39.457973	5
GTGGTAT	56585	0.0	39.33348	6
CGCAGAG	56790	0.0	39.24115	16
AGAGTAC	55285	0.0	39.136204	19
TATCAAC	57440	0.0	38.809364	10
CAACGCA	57775	0.0	38.787712	13
CAGTGGT	58285	0.0	38.319294	4
AACGCAG	58590	0.0	38.21207	14
CAGAGTA	57870	0.0	37.5667	18
GCAGAGT	58120	0.0	37.538544	17
AGCAGTG	60140	0.0	37.297558	2
GCAGTGG	61025	0.0	36.914543	3
GAGTACA	38200	0.0	36.031284	20
>>END_MODULE