FastQCFastQC Report
Thu 26 May 2016
SRR805367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19752145
Sequences flagged as poor quality0
Sequence length53
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3177471.6086708557475657No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC312210.15806384572409732No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG226340.11459008629189386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1655750.045.29684412
GTATCAA1654000.045.022259
ACGCAGA1662550.044.99849715
GGTATCA1659150.044.926418
ATCAACG1669950.044.87086511
AGAGTAC1622950.044.66440619
TGGTATC1668050.044.651487
AAGCAGT1678850.044.57841
CGCAGAG1676900.044.55316516
GTGGTAT1683600.044.265596
CAACGCA1695000.044.2396213
AGTGGTA1680450.044.23395
TATCAAC1686150.044.1791410
AACGCAG1700300.044.06992714
CAGAGTA1656350.043.99643718
GCAGAGT1674850.043.72934717
CAGTGGT1705200.043.7200364
GTACATG990800.043.62252822
GAGTACA1082200.043.4452520
ACATGGG1001150.043.2701424
Produced by FastQC (version 0.11.5)