##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR805367_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19752145 Sequences flagged as poor quality 0 Sequence length 53 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.07291056237183 35.0 35.0 37.0 33.0 37.0 2 35.29802753068085 36.0 35.0 37.0 33.0 37.0 3 37.028871345365275 39.0 37.0 39.0 33.0 39.0 4 37.03087750722769 39.0 37.0 39.0 33.0 39.0 5 37.02025830612321 39.0 37.0 39.0 33.0 39.0 6 36.948828494323024 39.0 37.0 39.0 33.0 39.0 7 36.79851671805771 39.0 37.0 39.0 33.0 39.0 8 37.71923864471428 40.0 37.0 40.0 33.0 40.0 9 37.696586016354175 40.0 37.0 40.0 33.0 40.0 10 37.43066269511488 39.0 37.0 40.0 32.0 40.0 11 37.56086617428132 40.0 37.0 40.0 33.0 40.0 12 37.55274239835724 40.0 37.0 40.0 33.0 40.0 13 37.65867534893046 40.0 37.0 40.0 33.0 40.0 14 37.59990249160281 40.0 37.0 40.0 33.0 40.0 15 37.4687598233002 40.0 37.0 40.0 32.0 40.0 16 37.39979394642962 40.0 37.0 40.0 32.0 40.0 17 37.29059238882663 39.0 37.0 40.0 32.0 40.0 18 37.25840985877736 39.0 37.0 40.0 32.0 40.0 19 37.159154663961814 39.0 36.0 40.0 32.0 40.0 20 36.98800545459746 39.0 36.0 40.0 31.0 40.0 21 36.80078168725473 39.0 36.0 40.0 31.0 40.0 22 36.82497991990237 39.0 36.0 40.0 31.0 40.0 23 36.37348581635058 39.0 36.0 40.0 30.0 40.0 24 36.45764153715964 39.0 36.0 40.0 30.0 40.0 25 36.45301930499194 39.0 36.0 40.0 30.0 40.0 26 30.933522511099426 38.0 29.0 40.0 3.0 40.0 27 32.78081914647751 38.0 29.0 40.0 16.0 40.0 28 34.37814131072853 38.0 30.0 40.0 25.0 40.0 29 35.35877040189812 38.0 34.0 40.0 27.0 40.0 30 35.71468455704431 38.0 34.0 40.0 29.0 40.0 31 35.69953217739137 38.0 35.0 40.0 28.0 40.0 32 35.61875406443199 38.0 34.0 40.0 27.0 40.0 33 35.600059740347184 38.0 34.0 40.0 28.0 40.0 34 35.50085983066649 38.0 34.0 40.0 27.0 40.0 35 35.46447649103426 38.0 34.0 40.0 27.0 40.0 36 35.32702848222307 38.0 34.0 40.0 27.0 40.0 37 35.17374016847284 38.0 34.0 40.0 27.0 40.0 38 35.00095407359555 38.0 33.0 40.0 26.0 40.0 39 34.91873682579791 38.0 33.0 40.0 26.0 40.0 40 34.82386399046787 38.0 33.0 40.0 26.0 40.0 41 34.75292349261308 37.0 33.0 40.0 26.0 40.0 42 34.58468080302165 37.0 33.0 40.0 25.0 40.0 43 34.510614720578445 37.0 33.0 40.0 26.0 40.0 44 34.37366493613732 37.0 33.0 40.0 25.0 40.0 45 34.165852873194275 37.0 32.0 39.0 24.0 40.0 46 33.94025666579503 36.0 32.0 39.0 24.0 40.0 47 34.08371460416071 37.0 32.0 39.0 24.0 40.0 48 34.085955221572135 37.0 32.0 39.0 24.0 40.0 49 33.92564397436329 36.0 32.0 39.0 24.0 40.0 50 33.763667085270995 36.0 32.0 39.0 23.0 40.0 51 33.57073563402861 36.0 31.0 39.0 23.0 40.0 52 33.30080130537721 36.0 31.0 39.0 23.0 40.0 53 32.81290766142108 35.0 31.0 38.0 22.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 7.0 11 27.0 12 57.0 13 134.0 14 311.0 15 833.0 16 1788.0 17 3946.0 18 8059.0 19 15192.0 20 26291.0 21 42250.0 22 63653.0 23 91935.0 24 126303.0 25 168761.0 26 218535.0 27 278369.0 28 348599.0 29 433826.0 30 534931.0 31 662661.0 32 824440.0 33 1040642.0 34 1341962.0 35 1774962.0 36 2382119.0 37 3147421.0 38 3504857.0 39 2709273.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.996416844854066 32.84669589049696 28.949514090748117 23.207373173900862 2 26.70610204613221 35.15446043961302 19.121032171442646 19.018405342812137 3 31.98237963522443 24.03519212723479 21.29348483417877 22.688943403362014 4 22.140177687030953 23.527849760114663 22.119658396594392 32.21231415625999 5 22.951264280411067 31.83793962630388 23.29159187521153 21.91920421807353 6 31.03090322595343 23.54696160847341 22.290627169859274 23.131507995713882 7 23.239562083004152 23.33536433637967 31.38487490852259 22.040198672093588 8 30.367992944563742 23.987754241374798 23.343449534215143 22.300803279846313 9 30.19755069639272 23.61840701351676 23.7410924231267 22.442949866963815 10 22.314938453519858 23.98171945376059 31.438813354195204 22.264528738524348 11 22.0861784884629 31.944768530202673 23.56651897806542 22.40253400326901 12 21.946289883959437 23.751050835238402 31.77958647022893 22.52307281057323 13 22.04299330528406 23.904608841216994 23.672573282547287 30.379824570951662 14 22.418557579442638 31.94858583713313 23.372529920168166 22.260326663256066 15 22.164514284397974 31.776189370825296 23.481358606875354 22.577937737901376 16 22.254231122746415 23.7835890734905 23.52851297922327 30.433666824539817 17 30.535038093331128 23.51355257871993 23.518478625992266 22.432930701956675 18 22.443648525261434 23.492359943692193 23.43489783008377 30.629093700962606 19 22.459170890047638 31.581623160421312 23.412677458574752 22.546528490956298 20 30.715666576971767 23.50210572066983 23.378574833264945 22.403652869093456 21 22.65863783401752 31.459722475710866 23.374666396991316 22.5069732932803 22 30.483767712316812 23.41774019986184 23.653177920676463 22.445314167144883 23 22.636245329304742 23.386012000215672 31.410117736580002 22.567624933899584 24 22.472885856194353 31.37469879853555 23.55915268949271 22.593262655777384 25 22.369383173321175 23.49117526223101 23.48669979893323 30.65274176551458 26 20.883053460776033 36.21192027498786 24.229115369495315 18.6759108947408 27 22.60696243370024 23.398339775249728 31.378592046585318 22.616105744464715 28 28.115483153854935 23.312131416613234 26.321384335726577 22.251001093805257 29 27.93181196270076 23.44529163794616 26.108247990281562 22.514648409071523 30 27.77249255713746 23.47610854416065 26.176397550747023 22.575001347954867 31 26.11243994006727 24.0225656504648 26.65583915063402 23.209155258833913 32 24.876634917372265 24.801311452503004 26.7190019109317 23.60305171919303 33 24.39195844299442 24.672970960875386 27.18839903210512 23.74667156402507 34 23.82491623061698 24.73814869220533 27.49800085003426 23.93893422714343 35 23.977147798378354 24.962590138944403 27.389121535914203 23.671140526763043 36 23.8797912834277 24.76801886579913 27.62076220076351 23.731427650009657 37 23.98754160624074 24.469686709975043 27.705770689714964 23.837000994069253 38 24.315338916355667 24.530242158509875 27.277022318335554 23.877396606798907 39 24.580596183351226 24.431797154182494 27.168431580468855 23.81917508199742 40 24.06232335779228 24.571113668920514 27.299485701426352 24.067077271860853 41 24.34149303784475 24.81813494179999 27.121656913717473 23.718715106637784 42 24.388095571392373 24.74952467187741 27.125772922383874 23.736606834346347 43 24.32309503600748 24.753458421857474 27.394741178742866 23.528705363392177 44 24.52499715853645 24.611549783580465 27.030066861092806 23.833386196790272 45 24.33762004076013 24.508452120010258 27.178491247406296 23.975436591823318 46 24.049494371370805 24.525098413362194 27.130825538188386 24.294581677078618 47 24.312660726214798 24.543116709602934 26.980284926016896 24.163937638165375 48 24.468253954190796 24.41008811954347 27.102160297020905 24.019497629244825 49 24.050592986230104 25.01889794753937 26.987322136405943 23.943186929824584 50 24.39027255014582 24.79520578651078 27.035028347554153 23.779493315789246 51 24.28069964046943 24.68578982181429 26.99646544717042 24.03704509054586 52 24.408230093491113 24.571812327218133 26.944040761142652 24.075916818148105 53 24.833586428208175 24.67965377937434 26.37484182097691 24.11191797144057 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4409.0 1 2712.0 2 1015.0 3 908.0 4 801.0 5 912.5 6 1024.0 7 1186.5 8 1755.5 9 2162.0 10 2797.0 11 3432.0 12 4733.0 13 6034.0 14 7912.0 15 9790.0 16 13641.5 17 17493.0 18 23850.5 19 30208.0 20 43846.5 21 57485.0 22 273971.5 23 490458.0 24 343935.5 25 195299.0 26 193185.0 27 209906.0 28 226627.0 29 248798.0 30 270969.0 31 297747.5 32 324526.0 33 367973.0 34 411420.0 35 464511.5 36 517603.0 37 577667.5 38 637732.0 39 700909.0 40 764086.0 41 829432.0 42 967375.5 43 1039973.0 44 1131889.0 45 1223805.0 46 1289469.5 47 1355134.0 48 1433889.5 49 1512645.0 50 1538157.0 51 1563669.0 52 1555896.5 53 1548124.0 54 1496446.0 55 1444768.0 56 1366620.5 57 1288473.0 58 1189860.0 59 986258.0 60 881269.0 61 758363.5 62 635458.0 63 539852.0 64 444246.0 65 364692.0 66 285138.0 67 228617.5 68 172097.0 69 135294.0 70 98491.0 71 76043.5 72 53596.0 73 39263.5 74 24931.0 75 18763.0 76 9341.5 77 6088.0 78 4579.0 79 3070.0 80 2398.0 81 1726.0 82 1308.0 83 890.0 84 640.0 85 390.0 86 274.5 87 159.0 88 126.0 89 93.0 90 70.0 91 47.0 92 32.5 93 10.5 94 3.0 95 2.5 96 2.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 53 1.9752145E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.954813557157976 #Duplication Level Percentage of deduplicated Percentage of total 1 75.28510567313774 36.102832061862564 2 12.716328846317806 12.196183579133603 3 4.4958667144257225 6.467953502043539 4 2.085594735132864 4.000572267163471 5 1.2078919674954134 2.896211704921564 6 0.7850609666192575 2.2588471371137233 7 0.5408927894822888 1.8156889011823978 8 0.3983818501218427 1.5283461877718894 9 0.29798177658735364 1.2860694485709523 >10 2.0313761047942007 18.072972197257204 >50 0.09927961293797764 3.1368904729938896 >100 0.04898460389933618 5.2548400375707525 >500 0.006244588944536236 1.94443111096381 >1k 8.917271890897503E-4 0.6767123655239049 >5k 4.292469620758441E-5 0.15025625143614363 >10k+ 7.511821836327223E-5 2.211192774490881 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 317747 1.6086708557475657 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC 31221 0.15806384572409732 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG 22634 0.11459008629189386 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.720897654406648E-4 5.062741287085529E-6 0.0 0.0 1.5188223861256588E-5 2 0.0013365636997905797 5.062741287085529E-6 0.0 0.0 1.5188223861256588E-5 3 0.0013466891823647508 5.062741287085529E-6 0.0 0.0 3.0376447722513176E-5 4 0.0013517519236518362 1.0125482574171059E-5 0.0 0.0 5.062741287085529E-5 5 0.0015998262467190273 1.0125482574171059E-5 0.0 0.0 8.606660188045399E-5 6 0.0016605791421640537 2.0250965148342117E-5 0.0 0.0 1.0631756702879611E-4 7 0.001665641883451139 2.0250965148342117E-5 0.0 0.0 1.0631756702879611E-4 8 0.0016757673660253102 2.0250965148342117E-5 0.0 0.0 1.1644304960296717E-4 9 0.0016808301073123956 2.0250965148342117E-5 0.0 0.0 1.3669401475130928E-4 10 0.001711206555034909 2.0250965148342117E-5 0.0 0.0 1.5188223861256587E-4 11 0.0017365202614703366 2.0250965148342117E-5 0.0 5.062741287085529E-6 1.6707046247382246E-4 12 0.001797273156915363 2.0250965148342117E-5 0.0 1.5188223861256588E-5 1.8225868633507905E-4 13 0.002202292459882205 2.0250965148342117E-5 0.0 1.5188223861256588E-5 2.075723927705067E-4 14 0.0023541746984947712 2.0250965148342117E-5 0.0 1.5188223861256588E-5 2.2782335791884882E-4 15 0.002860448827203324 2.0250965148342117E-5 0.0 5.062741287085529E-5 2.2782335791884882E-4 16 0.0029313272052225213 2.0250965148342117E-5 0.0 5.062741287085529E-5 2.3288609920593434E-4 17 0.002956640911657949 2.0250965148342117E-5 0.0 1.1138030831588164E-4 2.3794884049301986E-4 18 0.003224966199873482 2.0250965148342117E-5 0.0 1.1138030831588164E-4 2.531370643542765E-4 19 0.003280656354031423 2.0250965148342117E-5 0.0 1.1644304960296717E-4 2.5819980564136197E-4 20 0.0033059700604668504 2.0250965148342117E-5 0.0 1.1644304960296717E-4 2.632625469284475E-4 21 0.003341409249476449 2.0250965148342117E-5 0.0 1.1644304960296717E-4 2.784507707897041E-4 22 0.003386973921060219 2.5313706435427645E-5 0.0 1.1644304960296717E-4 3.240154423734739E-4 23 0.0034477268165052453 2.5313706435427645E-5 0.0 1.1644304960296717E-4 3.4426640752181596E-4 24 0.0035337934183856993 3.0376447722513176E-5 0.0 1.1644304960296717E-4 3.5945463138307255E-4 25 0.003645173726701581 3.0376447722513176E-5 0.0 1.1644304960296717E-4 3.5945463138307255E-4 26 0.0037565540350174625 3.543918900959871E-5 0.0 1.1644304960296717E-4 3.7464285524432914E-4 27 0.004055255770955509 3.543918900959871E-5 0.0 1.1644304960296717E-4 3.797055965314147E-4 28 0.004095757701252193 3.543918900959871E-5 0.0 1.1644304960296717E-4 4.050193029668423E-4 29 0.004414710402338581 3.543918900959871E-5 0.0 1.1644304960296717E-4 4.4045849197644105E-4 30 0.004510902486793206 3.543918900959871E-5 0.0 1.1644304960296717E-4 4.657721984118687E-4 31 0.004652659242831601 3.543918900959871E-5 0.0 1.1644304960296717E-4 4.758976809860397E-4 32 0.004764039551147483 3.543918900959871E-5 0.0 1.1644304960296717E-4 4.910859048472963E-4 33 0.0048501061530279365 3.543918900959871E-5 0.0 1.1644304960296717E-4 5.26525093856895E-4 34 0.004966549202630904 3.543918900959871E-5 0.0 1.1644304960296717E-4 5.619642828664938E-4 35 0.005133619665104726 4.556467158376976E-5 0.0 1.1644304960296717E-4 6.024662131631779E-4 36 0.00531081561015272 4.556467158376976E-5 0.0 1.1644304960296717E-4 6.277799195986057E-4 37 0.005467760590052372 4.556467158376976E-5 0.0 1.215057908900527E-4 6.581563673211187E-4 38 0.005655082017674536 4.556467158376976E-5 0.0 1.2656853217713824E-4 6.632191086082043E-4 39 0.0057867132911387596 4.556467158376976E-5 0.0 1.2656853217713824E-4 6.834700737565465E-4 40 0.005923407305890069 4.556467158376976E-5 0.0 1.2656853217713824E-4 6.935955563307175E-4 41 0.006044913096780122 4.556467158376976E-5 0.0 1.2656853217713824E-4 7.290347453403162E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 165575 0.0 45.296844 12 GTATCAA 165400 0.0 45.02225 9 ACGCAGA 166255 0.0 44.998497 15 GGTATCA 165915 0.0 44.92641 8 ATCAACG 166995 0.0 44.870865 11 AGAGTAC 162295 0.0 44.664406 19 TGGTATC 166805 0.0 44.65148 7 AAGCAGT 167885 0.0 44.5784 1 CGCAGAG 167690 0.0 44.553165 16 GTGGTAT 168360 0.0 44.26559 6 CAACGCA 169500 0.0 44.23962 13 AGTGGTA 168045 0.0 44.2339 5 TATCAAC 168615 0.0 44.17914 10 AACGCAG 170030 0.0 44.069927 14 CAGAGTA 165635 0.0 43.996437 18 GCAGAGT 167485 0.0 43.729347 17 CAGTGGT 170520 0.0 43.720036 4 GTACATG 99080 0.0 43.622528 22 GAGTACA 108220 0.0 43.44525 20 ACATGGG 100115 0.0 43.27014 24 >>END_MODULE