FastQCFastQC Report
Thu 26 May 2016
SRR805366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24680379
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7908413.204330857317872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG533510.2161676690621323No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG395490.16024470288726117No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG364070.14751394214813315No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA355730.1441347395840234No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG348640.14126201222436657No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC338990.1373520236459902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2739950.045.1679951
GGTATCA2732350.044.8137138
GTATCAA2732750.044.8080149
TCAACGC2747050.044.74841712
TGGTATC2742300.044.7299547
ATCAACG2758200.044.52492511
ACGCAGA2774300.044.48338315
GTGGTAT2767400.044.477956
AGTGGTA2768350.044.372715
CGCAGAG2785750.044.2549916
AGAGTAC2732000.044.08830319
TATCAAC2787400.043.911810
CAGTGGT2807400.043.8768734
CAGAGTA2759850.043.77538318
GAGTACT1272350.043.70868720
CAACGCA2816350.043.67903513
AGCAGTG2837500.043.6491242
GCAGTGG2838200.043.6126943
AACGCAG2831400.043.5240514
GCAGAGT2786350.043.5125517
Produced by FastQC (version 0.11.5)