##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805366_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24680379
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.61268710662831	37.0	35.0	37.0	35.0	37.0
2	35.73324185985961	37.0	35.0	37.0	35.0	37.0
3	37.37901318290128	39.0	37.0	39.0	34.0	39.0
4	37.390759112734855	39.0	37.0	39.0	34.0	39.0
5	37.33418915487481	39.0	37.0	39.0	34.0	39.0
6	37.311373378828584	39.0	37.0	39.0	34.0	39.0
7	37.084612355426145	39.0	37.0	39.0	33.0	39.0
8	38.026117386609016	40.0	38.0	40.0	34.0	40.0
9	38.00582373552692	40.0	38.0	40.0	33.0	40.0
10	37.66393368594542	40.0	37.0	40.0	33.0	40.0
11	37.811951145482816	40.0	37.0	40.0	33.0	40.0
12	37.84617886135379	40.0	38.0	40.0	33.0	40.0
13	37.87416194054394	40.0	38.0	40.0	33.0	40.0
14	37.767839019003716	40.0	38.0	40.0	33.0	40.0
15	37.72922372869558	40.0	37.0	40.0	33.0	40.0
16	37.66183339404958	40.0	37.0	40.0	33.0	40.0
17	37.57537106703264	40.0	37.0	40.0	32.0	40.0
18	37.538512435323625	40.0	37.0	40.0	32.0	40.0
19	37.49025693649194	40.0	37.0	40.0	32.0	40.0
20	37.31335329169783	40.0	37.0	40.0	32.0	40.0
21	37.167894950073496	39.0	37.0	40.0	32.0	40.0
22	37.17139955589823	39.0	37.0	40.0	32.0	40.0
23	36.6811807873777	39.0	36.0	40.0	30.0	40.0
24	36.87137182941964	39.0	36.0	40.0	31.0	40.0
25	36.88427924871008	39.0	36.0	40.0	31.0	40.0
26	36.85517989816931	39.0	36.0	40.0	31.0	40.0
27	36.81033662408507	39.0	36.0	40.0	30.0	40.0
28	36.71312401645048	39.0	36.0	40.0	30.0	40.0
29	36.60555727284415	39.0	36.0	40.0	30.0	40.0
30	36.50592918366448	39.0	36.0	40.0	30.0	40.0
31	36.30467733092753	39.0	35.0	40.0	30.0	40.0
32	36.163531321784	38.0	35.0	40.0	30.0	40.0
33	36.017537291465416	38.0	35.0	40.0	29.0	40.0
34	35.87787946854463	38.0	35.0	40.0	29.0	40.0
35	35.7228407229889	38.0	35.0	40.0	28.0	40.0
36	35.863496707242625	38.0	35.0	40.0	29.0	40.0
37	35.80200125776027	38.0	35.0	40.0	29.0	40.0
38	35.75485303527957	38.0	35.0	40.0	29.0	40.0
39	35.72914358405922	38.0	34.0	40.0	29.0	40.0
40	35.698046452204	38.0	34.0	40.0	29.0	40.0
41	35.6462224911538	38.0	34.0	40.0	28.0	40.0
42	35.547469185947264	38.0	34.0	40.0	28.0	40.0
43	35.43145796099809	38.0	34.0	40.0	28.0	40.0
44	35.48727614758266	38.0	34.0	40.0	28.0	40.0
45	35.432143809460946	38.0	34.0	40.0	28.0	40.0
46	35.401818748407386	38.0	34.0	40.0	28.0	40.0
47	35.382307824365256	38.0	34.0	40.0	28.0	40.0
48	35.255720141088595	38.0	34.0	40.0	27.0	40.0
49	35.07325009068945	37.0	34.0	40.0	27.0	40.0
50	34.83978994001672	37.0	33.0	40.0	26.0	40.0
51	34.62500162578541	37.0	33.0	40.0	26.0	40.0
52	34.289989306890305	37.0	33.0	40.0	24.0	40.0
53	33.863112596447564	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	8.0
12	20.0
13	92.0
14	238.0
15	602.0
16	1500.0
17	3630.0
18	7532.0
19	14136.0
20	25130.0
21	40090.0
22	59653.0
23	85400.0
24	118232.0
25	158349.0
26	204611.0
27	259920.0
28	323991.0
29	401051.0
30	495827.0
31	613108.0
32	764918.0
33	968666.0
34	1269327.0
35	1728438.0
36	2458502.0
37	3673105.0
38	5124281.0
39	5880022.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.004090374787195	39.80210352523355	25.199479311075407	22.994326788903848
2	27.066946581330864	43.44289040293911	13.915637195036593	15.574525820693436
3	35.28186094711106	25.33076578767287	19.01596405792634	20.371409207289727
4	21.154662981472043	23.628247362003638	20.258497651109813	34.9585920054145
5	20.15549680173064	34.87733312361208	23.257462942526125	21.709707132131157
6	32.044714548346285	23.116808700547104	21.868262233736363	22.970214517370255
7	22.244257270117288	22.75876314541199	33.1373314810117	21.85964810345903
8	31.515148126371965	24.03348830259049	23.155754617868713	21.29560895316883
9	32.073543116983736	23.326712284280564	23.157205162854268	21.442539435881432
10	21.33390658222874	24.134471354755128	33.47616744459232	21.05545461842381
11	21.02397211971502	34.684674007639835	23.177083301678632	21.114270570966514
12	20.946011404443993	23.641926244325504	33.97737935872055	21.43468299250996
13	21.204978254183214	23.785684166357413	23.303394165867548	31.705943413591825
14	21.515674455404433	34.496885157233606	22.987637264403435	20.999803122958525
15	21.1651449923034	34.455228584617764	22.9177963596102	21.461830063468636
16	21.403913610889038	23.58322779403023	23.076513533280828	31.936345061799905
17	32.14925913414863	23.436933444174418	23.054374489143786	21.359432932533167
18	21.336066192500528	23.388222684910957	23.058191286284544	32.21751983630397
19	21.475598085426483	34.20664650247065	22.912273753980845	21.405481658122024
20	32.4694122403874	23.59091811353464	22.93949375736896	21.000175888709002
21	21.66938765405507	34.063743510583855	22.94220441266319	21.324664422697882
22	32.09628587956449	23.401784064985385	23.159004162780484	21.34292589266964
23	21.561553005324594	23.451840022391877	33.8149183203386	21.17168865194493
24	21.48925670874017	33.964073242149155	23.09739651891083	21.44927353019984
25	21.31706729463109	23.527511469738773	23.034480143112877	32.120941092517256
26	21.46628704526782	28.988521610628425	28.076918916034476	21.468272428069277
27	21.651948699815346	23.311380266891362	33.62279404218225	21.413876991111035
28	27.347772900894267	23.331955315597057	27.919834618423	21.400437165085673
29	27.47063973369291	23.41861119717813	27.785983351390186	21.324765717738774
30	27.37878133881169	23.320091640407952	27.848158247488826	21.452968773291527
31	25.469292833793194	23.952743999595793	28.373142081813253	22.204821084797764
32	24.367073131251345	24.755961000436823	28.620524830676224	22.256441037635604
33	24.032288969306347	24.463882827731293	28.951317968010137	22.552510234952226
34	23.144356899867706	24.672988206542534	29.113341411815437	23.06931348177433
35	22.980064447146457	24.898041476591587	29.37406269166288	22.747831384599078
36	23.261786214871336	24.599213002361108	29.669430927296535	22.46956985547102
37	23.38144402077456	24.382960245464627	29.645768405744498	22.589827328016316
38	23.397075871484795	24.4845389124697	29.419256487106622	22.699128728938884
39	23.822814876546264	24.38865302676268	29.201330336134628	22.587201760556432
40	23.109345281934285	24.92201598686957	29.211395011397517	22.75724371979863
41	23.48144248514174	24.998165546809474	29.072495199526717	22.44789676852207
42	23.456544164090833	24.976962468850257	29.042337639952777	22.524155727106134
43	23.18415774733443	25.00830720630344	29.47659758385396	22.330937462508174
44	23.467208505995796	24.77095266648863	29.144366056939404	22.61747277057617
45	23.50544535803117	24.501596997355673	29.217148569720102	22.775809074893054
46	23.028880553252442	24.792714893073562	29.042568592646006	23.13583596102799
47	23.098348692295204	24.767512686899988	29.032665989448542	23.101472631356266
48	23.257276559650887	24.373681619719047	29.18908984339341	23.179951977236655
49	22.867031336917478	25.280673364051665	28.86847077996655	22.983824519064314
50	23.426066512187678	25.090007734484143	28.809873624712168	22.674052128616015
51	23.358008400114112	24.843062580197817	28.898887654845172	22.9000413648429
52	23.681370533248295	24.72080756944616	28.63951562494239	22.95830627236316
53	24.13676467448089	24.955698613866506	27.825160221405028	23.082376490247576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14770.0
1	8743.0
2	2716.0
3	2361.5
4	2007.0
5	2142.0
6	2277.0
7	2669.5
8	3510.5
9	3959.0
10	5584.0
11	7209.0
12	9903.0
13	12597.0
14	15554.0
15	18511.0
16	25916.5
17	33322.0
18	44373.0
19	55424.0
20	77913.0
21	100402.0
22	566466.0
23	1032530.0
24	691675.0
25	337376.0
26	323932.0
27	342310.5
28	360689.0
29	386580.5
30	412472.0
31	440026.5
32	467581.0
33	520939.5
34	574298.0
35	634991.0
36	695684.0
37	767072.5
38	838461.0
39	909547.5
40	980634.0
41	1046738.5
42	1195591.5
43	1278340.0
44	1394752.5
45	1511165.0
46	1572577.0
47	1633989.0
48	1732636.0
49	1831283.0
50	1856897.0
51	1882511.0
52	1855051.5
53	1827592.0
54	1743401.0
55	1659210.0
56	1590355.0
57	1521500.0
58	1376631.0
59	1110472.0
60	989182.0
61	845933.5
62	702685.0
63	599009.5
64	495334.0
65	407780.0
66	320226.0
67	252623.0
68	185020.0
69	143092.0
70	101164.0
71	79325.5
72	57487.0
73	40343.0
74	23199.0
75	16974.5
76	8008.0
77	5266.0
78	4106.0
79	2946.0
80	2458.5
81	1971.0
82	1485.0
83	999.0
84	660.5
85	322.0
86	225.0
87	128.0
88	98.0
89	68.0
90	51.0
91	34.0
92	34.5
93	18.5
94	2.0
95	2.5
96	3.0
97	3.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.4680379E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.46149770447544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.38964534300484	23.842044035704344
2	16.578146606632142	12.089281088843505
3	6.924761507786631	7.574615274606064
4	3.449717473325535	5.031274629389913
5	1.9087289600407034	3.4797558297494953
6	1.1650338938538147	2.5487328387832164
7	0.7840958968353021	2.0012517519784288
8	0.5579374412228335	1.6274587785909795
9	0.385713769972113	1.2657331564580487
>10	2.586931292872499	18.37296163008757
>50	0.1769999593107642	4.373873340200277
>100	0.07140927486055344	5.091747560130392
>500	0.012533712631779392	3.2667551833487454
>1k	0.008099987012794838	4.607326991354144
>5k	1.0017844278455723E-4	0.24919231353765073
>10k+	1.4470219513318657E-4	4.57799559723726
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	790841	3.204330857317872	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	53351	0.2161676690621323	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG	39549	0.16024470288726117	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	36407	0.14751394214813315	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	35573	0.1441347395840234	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	34864	0.14126201222436657	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	33899	0.1373520236459902	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0939864416182588E-4	0.0	0.0	0.0	8.103603271246362E-6
2	0.004785177731670976	0.0	0.0	0.0	8.103603271246362E-6
3	0.004813540343120338	0.0	0.0	0.0	8.103603271246362E-6
4	0.005194409696868917	0.0	0.0	4.051801635623181E-6	8.103603271246362E-6
5	0.005198461498504541	0.0	0.0	4.051801635623181E-6	1.2155404906869541E-5
6	0.005198461498504541	0.0	0.0	4.051801635623181E-6	1.2155404906869541E-5
7	0.005214668705047033	4.051801635623181E-6	0.0	4.051801635623181E-6	1.2155404906869541E-5
8	0.005218720506682656	4.051801635623181E-6	0.0	4.051801635623181E-6	1.2155404906869541E-5
9	0.005218720506682656	4.051801635623181E-6	0.0	4.051801635623181E-6	1.6207206542492723E-5
10	0.005234927713225149	8.103603271246362E-6	0.0	8.103603271246362E-6	1.6207206542492723E-5
11	0.0052389795148607725	8.103603271246362E-6	0.0	8.103603271246362E-6	1.6207206542492723E-5
12	0.005283549332852628	1.2155404906869541E-5	0.0	8.103603271246362E-6	1.6207206542492723E-5
13	0.00538889617537883	1.6207206542492723E-5	0.0	8.103603271246362E-6	1.6207206542492723E-5
14	0.005405103381921323	1.6207206542492723E-5	0.0	1.2155404906869541E-5	2.8362611449362266E-5
15	0.00550234662117628	1.6207206542492723E-5	0.0	1.2155404906869541E-5	2.8362611449362266E-5
16	0.005514502026083149	1.6207206542492723E-5	0.0	1.2155404906869541E-5	2.8362611449362266E-5
17	0.005526657430990019	1.6207206542492723E-5	0.0	1.2155404906869541E-5	2.8362611449362266E-5
18	0.00556717544734625	2.0259008178115903E-5	0.0	1.2155404906869541E-5	2.8362611449362266E-5
19	0.00557933085225312	2.8362611449362266E-5	0.0	1.2155404906869541E-5	2.8362611449362266E-5
20	0.005595538058795612	3.2414413084985446E-5	0.0	1.2155404906869541E-5	3.2414413084985446E-5
21	0.005627952471880598	3.2414413084985446E-5	0.0	1.2155404906869541E-5	3.2414413084985446E-5
22	0.00565631508332996	3.2414413084985446E-5	0.0	1.2155404906869541E-5	4.0518016356231806E-5
23	0.0056765740915080765	3.2414413084985446E-5	0.0	1.2155404906869541E-5	4.8621619627478166E-5
24	0.005700884901321816	3.2414413084985446E-5	0.0	1.2155404906869541E-5	4.8621619627478166E-5
25	0.005741402917678047	3.2414413084985446E-5	0.0	1.2155404906869541E-5	5.672522289872453E-5
26	0.005781920934034279	3.2414413084985446E-5	0.0	1.2155404906869541E-5	5.672522289872453E-5
27	0.005846749760204249	3.2414413084985446E-5	0.0	1.2155404906869541E-5	5.672522289872453E-5
28	0.005907526784738598	3.646621472060862E-5	0.0	1.2155404906869541E-5	5.672522289872453E-5
29	0.006025029032171669	3.646621472060862E-5	0.0	1.2155404906869541E-5	5.672522289872453E-5
30	0.0060898578583416406	4.0518016356231806E-5	0.0	1.2155404906869541E-5	5.672522289872453E-5
31	0.006183049295960974	4.0518016356231806E-5	0.0	1.2155404906869541E-5	5.672522289872453E-5
32	0.006227619113952828	4.456981799185499E-5	0.0	1.2155404906869541E-5	5.672522289872453E-5
33	0.00628029253521593	4.456981799185499E-5	0.0	1.2155404906869541E-5	6.077702453434771E-5
34	0.006337017758114655	4.8621619627478166E-5	0.0	1.6207206542492723E-5	6.482882616997089E-5
35	0.006422105592462741	4.8621619627478166E-5	0.0	1.6207206542492723E-5	6.888062780559407E-5
36	0.0064909862202683355	4.8621619627478166E-5	0.0	1.6207206542492723E-5	7.698423107684044E-5
37	0.006551763244802683	4.8621619627478166E-5	0.0	1.6207206542492723E-5	8.508783434808679E-5
38	0.006653058285693262	4.8621619627478166E-5	0.0	1.6207206542492723E-5	8.508783434808679E-5
39	0.006754353326583842	5.267342126310135E-5	0.0	1.6207206542492723E-5	8.913963598370998E-5
40	0.006924528995280016	5.267342126310135E-5	0.0	1.6207206542492723E-5	8.913963598370998E-5
41	0.007062290250891204	5.267342126310135E-5	0.0	1.6207206542492723E-5	8.913963598370998E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	273995	0.0	45.167995	1
GGTATCA	273235	0.0	44.813713	8
GTATCAA	273275	0.0	44.808014	9
TCAACGC	274705	0.0	44.748417	12
TGGTATC	274230	0.0	44.729954	7
ATCAACG	275820	0.0	44.524925	11
ACGCAGA	277430	0.0	44.483383	15
GTGGTAT	276740	0.0	44.47795	6
AGTGGTA	276835	0.0	44.37271	5
CGCAGAG	278575	0.0	44.25499	16
AGAGTAC	273200	0.0	44.088303	19
TATCAAC	278740	0.0	43.9118	10
CAGTGGT	280740	0.0	43.876873	4
CAGAGTA	275985	0.0	43.775383	18
GAGTACT	127235	0.0	43.708687	20
CAACGCA	281635	0.0	43.679035	13
AGCAGTG	283750	0.0	43.649124	2
GCAGTGG	283820	0.0	43.612694	3
AACGCAG	283140	0.0	43.52405	14
GCAGAGT	278635	0.0	43.51255	17
>>END_MODULE