FastQCFastQC Report
Thu 26 May 2016
SRR805365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17712928
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360182.4615806037262726No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG528790.2985333650088794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG361710.20420678049388558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC346100.19539400826334305No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG218370.12328283612963369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC2606650.045.55212812
GGTATCA2595800.045.5225378
TGGTATC2593000.045.5200357
GTATCAA2595200.045.5131389
ATCAACG2611600.045.384811
ACGCAGA2602750.045.3729915
AAGCAGT2308700.045.336771
GTGGTAT2297850.045.3064166
CAACGCA2616100.045.29236213
AGTGGTA2297750.045.2173655
AACGCAG2636100.045.17605614
CGCAGAG2608900.045.12641516
TATCAAC2617500.045.11640510
AGAGTAC2545700.045.0743619
CAGAGTA2573400.044.75538318
GCAGAGT2597200.044.67189417
CAGTGGT2338950.044.5444564
AGCAGTG2354800.044.507092
GTACATG1746750.044.4976422
GAGTACA1762450.044.45459720