FastQCFastQC Report
Thu 26 May 2016
SRR805364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19192054
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT777710.4052249957195827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC447550.041.12959712
CAACGCA457850.040.17866513
ATCAACG459300.040.0364711
ACGCAGA458750.040.0076315
GGTATCA455300.039.9081958
GTATCAA460850.039.677459
AACGCAG468350.039.28792614
GTGGTAT465400.039.0825086
TGGTATC465100.039.047097
CGCAGAG470600.038.9952216
AGTGGTA470900.038.5761345
AAGCAGT476200.038.3984681
TATCAAC477400.038.3265610
AGAGTAC466450.038.31943519
CAGAGTA491500.036.5863718
CAGTGGT502500.036.3373154
GCAGAGT500650.036.1242417
GAGTACA340650.035.68633720
GTACATG337900.035.51775722
AGCAGTG518950.035.2896272
Produced by FastQC (version 0.11.5)