##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805364_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19192054
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.51171469192406	36.0	35.0	37.0	33.0	37.0
2	35.58248127063419	37.0	35.0	37.0	33.0	37.0
3	37.25572906370522	39.0	37.0	39.0	34.0	39.0
4	37.32928096179804	39.0	37.0	39.0	34.0	39.0
5	37.30402775023455	39.0	37.0	39.0	34.0	39.0
6	37.255461974002365	39.0	37.0	39.0	34.0	39.0
7	37.17388857909633	39.0	37.0	39.0	33.0	39.0
8	37.977677636796976	40.0	38.0	40.0	33.0	40.0
9	37.952910251294625	40.0	38.0	40.0	33.0	40.0
10	37.876604505176985	40.0	38.0	40.0	33.0	40.0
11	37.87139312967752	40.0	38.0	40.0	33.0	40.0
12	37.7792161276745	40.0	37.0	40.0	33.0	40.0
13	37.89961480933724	40.0	38.0	40.0	33.0	40.0
14	37.77203570811128	40.0	37.0	40.0	33.0	40.0
15	37.744740140893725	40.0	37.0	40.0	33.0	40.0
16	37.64796983168138	40.0	37.0	40.0	33.0	40.0
17	37.54347356463253	40.0	37.0	40.0	32.0	40.0
18	37.49166910430744	40.0	37.0	40.0	32.0	40.0
19	37.385706553347546	40.0	37.0	40.0	32.0	40.0
20	37.23149580550368	39.0	37.0	40.0	32.0	40.0
21	37.17742092638964	39.0	37.0	40.0	32.0	40.0
22	37.09613457736207	39.0	37.0	40.0	32.0	40.0
23	36.940199105317234	39.0	36.0	40.0	31.0	40.0
24	36.931121077504265	39.0	36.0	40.0	31.0	40.0
25	36.851810650386874	39.0	36.0	40.0	31.0	40.0
26	36.71171657812134	39.0	36.0	40.0	30.0	40.0
27	36.65488295312216	39.0	36.0	40.0	30.0	40.0
28	36.483418450156506	39.0	36.0	40.0	30.0	40.0
29	36.38542716688897	39.0	35.0	40.0	30.0	40.0
30	36.28642869595927	38.0	35.0	40.0	30.0	40.0
31	36.178435825576564	38.0	35.0	40.0	30.0	40.0
32	36.07691818707888	38.0	35.0	40.0	30.0	40.0
33	35.98391339457465	38.0	35.0	40.0	29.0	40.0
34	35.85703729262121	38.0	35.0	40.0	29.0	40.0
35	35.668551213955524	38.0	34.0	40.0	28.0	40.0
36	35.64221307422332	38.0	34.0	40.0	28.0	40.0
37	35.543633370352126	38.0	34.0	40.0	27.0	40.0
38	35.71736516581289	38.0	35.0	40.0	28.0	40.0
39	35.80114494258926	38.0	35.0	40.0	29.0	40.0
40	35.79032635068659	38.0	35.0	40.0	28.0	40.0
41	35.78322117059487	38.0	35.0	40.0	28.0	40.0
42	35.71014358338091	38.0	34.0	40.0	28.0	40.0
43	35.67225410057725	38.0	34.0	40.0	28.0	40.0
44	35.53894877536297	38.0	34.0	40.0	28.0	40.0
45	35.417141333595666	38.0	34.0	40.0	27.0	40.0
46	35.28030850684351	38.0	34.0	40.0	27.0	40.0
47	35.17117224659747	38.0	34.0	40.0	27.0	40.0
48	35.11786497682843	38.0	34.0	40.0	27.0	40.0
49	34.953887530745796	38.0	33.0	40.0	26.0	40.0
50	34.82242994939468	37.0	33.0	40.0	26.0	40.0
51	34.61545366639756	37.0	33.0	40.0	26.0	40.0
52	34.417095220761674	37.0	33.0	40.0	25.0	40.0
53	34.21675486115243	37.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	8.0
12	17.0
13	61.0
14	148.0
15	415.0
16	1172.0
17	2644.0
18	5820.0
19	11355.0
20	20525.0
21	32685.0
22	48942.0
23	69935.0
24	94494.0
25	124518.0
26	160286.0
27	201992.0
28	254463.0
29	316597.0
30	393461.0
31	487615.0
32	606228.0
33	764358.0
34	987760.0
35	1325196.0
36	1864092.0
37	2777481.0
38	4275174.0
39	4364610.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.907807470737628	27.152950903535388	28.852539702107965	28.08670192361901
2	24.767547027535457	25.49052852810856	28.891404744901195	20.850519699454782
3	19.97566284463351	24.58094375932873	30.211128001202997	25.23226539483476
4	19.484105244806003	26.840623728966168	29.430075592742703	24.24519543348513
5	24.05908716180144	28.25163997558573	27.351126669401825	20.338146193211003
6	24.43842644461088	25.839959599946937	27.68467095809547	22.036942997346713
7	23.604581354345918	26.391463883959474	28.879071515742922	21.124883245951683
8	23.705831590511366	25.086851047834692	27.641590629121822	23.56572673253212
9	23.734624756683157	24.66842788166394	28.558694134562145	23.038253227090753
10	22.814170906355308	25.501871764220756	28.62060517337019	23.063352156053753
11	22.586644451917444	27.772926232908684	26.6567715993296	22.983657715844277
12	22.61253016482759	25.782456635438812	28.491848761992856	23.113164437740743
13	22.87129350511415	25.893783958715417	26.14410109517199	25.090821440998447
14	23.13155225594926	27.826015912627174	25.986968356800162	23.0554634746234
15	22.82510772426964	27.871071017203263	26.05970158274878	23.244119675778318
16	23.103905397515035	25.866366361828703	25.844273885431964	25.1854543552243
17	25.44010140863505	25.58722479626204	25.803157911081325	23.169515884021585
18	23.189659637264466	25.5417372210395	25.77171260564398	25.496890536052057
19	23.416117941310503	27.50552390067264	25.733738556592222	23.344619601424633
20	25.426960553570765	25.542508373517496	25.707430794015064	23.32310027889667
21	23.422641474435203	27.531268930360454	25.74728061936466	23.298808975839687
22	25.4238863646382	25.468957100683436	25.750865436289416	23.356291098388947
23	23.429795476815563	25.528523419119182	27.839141136222313	23.20253996784294
24	23.251893726434908	27.532383975159718	25.807263776977702	23.40845852142767
25	23.30082543535986	25.51624750534779	25.70882720525901	25.474099854033337
26	23.433171874151668	26.833381148260628	26.426572163667316	23.306874813920388
27	23.354681057066635	25.433713348242975	27.820070743860974	23.39153485082941
28	24.990404883187594	25.35228902544772	26.28831181904761	23.368994272317074
29	24.998220617761913	25.479049819263743	26.178907166476296	23.34382239649805
30	24.835830495266425	25.37749737469476	26.24867562377638	23.537996506262434
31	24.476869437737097	25.60507593402978	26.213582975537687	23.70447165269543
32	24.265990497942532	25.938005384936915	26.188431941677532	23.607572175443025
33	24.01353706070231	25.81254721354994	26.303208609146267	23.870707116601487
34	24.008425570290704	25.74669704451644	26.2886296589203	23.95624772627255
35	24.193882530759865	25.817122023520778	26.31254580671772	23.67644963900164
36	23.935291136633943	25.813964466752754	26.366771373194343	23.883973023418964
37	24.107206034330666	25.737500530167328	26.23226779166003	23.923025643841978
38	24.208112378174842	25.87479172369982	26.262483421524347	23.654612476600995
39	24.10328253557436	25.757081550520855	26.313212749401394	23.826423164503392
40	24.1121664205405	25.79195535819147	26.166141466671572	23.92973675459646
41	24.111801686260364	25.951604763096224	26.261576796313722	23.675016754329683
42	24.016652933552603	25.837614879574637	26.358955638620024	23.786776548252732
43	24.055533607814986	25.757811019081128	26.27433207513901	23.91232329796488
44	24.195482151102745	25.756753289668733	26.233085838545474	23.814678720683048
45	24.084618561410885	25.598302297398707	26.33343987047973	23.98363927071068
46	24.017398033582023	25.68087292793153	26.198107821080534	24.103621217405912
47	24.12650568823952	25.74940128867916	26.198811237192228	23.925281785889098
48	23.999374949653642	25.679127413876596	26.272341668067423	24.04915596840234
49	24.05988957721774	25.869221710193184	26.007763421257568	24.063125291331506
50	24.219653612896252	25.85985846017315	26.06635537811638	23.854132548814214
51	24.108091817582423	25.700250739186124	26.17895406088374	24.012703382347716
52	24.255137047863663	25.72649076539697	25.925307421498502	24.093064765240864
53	24.28560278123436	25.80410100971996	25.918559837316007	23.991736371729676
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12755.0
1	7916.5
2	3078.0
3	2624.5
4	2171.0
5	2303.0
6	2435.0
7	2844.0
8	4056.5
9	4860.0
10	6270.0
11	7680.0
12	10267.5
13	12855.0
14	16407.0
15	19959.0
16	27020.5
17	34082.0
18	44383.0
19	54684.0
20	73353.5
21	92023.0
22	157089.5
23	222156.0
24	209859.0
25	220481.5
26	243401.0
27	273800.0
28	304199.0
29	334312.0
30	364425.0
31	393035.0
32	421645.0
33	467845.0
34	514045.0
35	566951.0
36	619857.0
37	680562.0
38	741267.0
39	800262.5
40	859258.0
41	921060.0
42	1042698.0
43	1102534.0
44	1168732.0
45	1234930.0
46	1281365.5
47	1327801.0
48	1368200.0
49	1408599.0
50	1421080.5
51	1433562.0
52	1417487.0
53	1401412.0
54	1346173.5
55	1290935.0
56	1209228.0
57	1127521.0
58	1034125.0
59	838958.0
60	737187.0
61	640976.0
62	544765.0
63	463178.0
64	381591.0
65	310809.5
66	240028.0
67	192672.5
68	145317.0
69	112757.0
70	80197.0
71	60028.0
72	39859.0
73	29164.5
74	18470.0
75	13779.0
76	6499.0
77	3910.0
78	2787.0
79	1664.0
80	1229.0
81	794.0
82	607.0
83	420.0
84	273.5
85	127.0
86	89.0
87	51.0
88	36.5
89	22.0
90	16.0
91	10.0
92	10.5
93	9.5
94	8.0
95	4.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.9192054E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.05700708643512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.120090084873	38.8646397888344
2	12.340346112406467	12.601222778203983
3	4.32102628370247	6.618560087630083
4	2.0640463854203	4.215361237085402
5	1.1740244255522516	2.997108670753462
6	0.7618887250619831	2.3339854820738815
7	0.5252235190975371	1.8771438655567745
8	0.4000676852944725	1.6341006914506864
9	0.31085956955204114	1.428440332095426
>10	1.8187135723377914	17.165710125105825
>50	0.11007040983890333	3.8358440555758535
>100	0.050462907841201755	4.6535219495943725
>500	0.002852406782091582	0.9622216552277046
>1k	2.76740870229292E-4	0.21769189231334776
>5k	3.0702821574129886E-5	0.1291436337539087
>10k+	2.0468547716086506E-5	0.465303754744812
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	77771	0.4052249957195827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0420979432425523E-4	0.0	0.0	0.0	3.647342801348933E-5
2	1.3026224290531904E-4	1.5631469148638286E-5	0.0	0.0	4.689440744591486E-5
3	1.354727326215318E-4	2.0841958864851047E-5	0.0	0.0	7.294685602697866E-5
4	1.354727326215318E-4	2.0841958864851047E-5	0.0	0.0	1.0420979432425523E-4
5	4.897860333239996E-4	2.0841958864851047E-5	0.0	0.0	1.1984126347289352E-4
6	5.054175024726379E-4	2.0841958864851047E-5	0.0	0.0	1.4589371205395732E-4
7	5.158384819050634E-4	2.0841958864851047E-5	0.0	0.0	1.615251812025956E-4
8	5.210489716212761E-4	2.0841958864851047E-5	0.0	0.0	1.6673567091880838E-4
9	5.418909304861272E-4	2.605244858106381E-5	0.0	0.0	1.9799860921608495E-4
10	5.783643584996165E-4	2.605244858106381E-5	0.0	0.0	2.0841958864851047E-4
11	6.096272967968931E-4	2.605244858106381E-5	0.0	0.0	2.2405105779714875E-4
12	6.825741528238717E-4	2.605244858106381E-5	0.0	0.0	2.709454652430636E-4
13	7.65941988283276E-4	2.605244858106381E-5	0.0	0.0	2.865769343917019E-4
14	8.128363957291909E-4	2.605244858106381E-5	0.0	0.0	3.0741889325655293E-4
15	8.753622723237439E-4	2.605244858106381E-5	0.0	0.0	3.0741889325655293E-4
16	9.274671694858716E-4	2.605244858106381E-5	0.0	0.0	3.0741889325655293E-4
17	9.691510872155736E-4	2.605244858106381E-5	0.0	0.0	3.4910281098625505E-4
18	0.001005624515229063	2.605244858106381E-5	0.0	0.0	3.699447698511061E-4
19	0.0010212559843777013	2.605244858106381E-5	0.0	0.0	3.8557623899974436E-4
20	0.0010368874535263395	2.605244858106381E-5	0.0	0.0	4.2204966701323373E-4
21	0.0010837818609722545	2.605244858106381E-5	0.0	0.0	4.481021155942975E-4
22	0.0011202552889857439	2.605244858106381E-5	0.0	0.0	4.5852309502672305E-4
23	0.0011723601861478713	2.605244858106381E-5	0.0	0.0	4.793650538915741E-4
24	0.0011984126347289353	2.605244858106381E-5	0.0	0.0	4.845755436077868E-4
25	0.0012453070421748502	2.605244858106381E-5	0.0	0.0	5.002070127564252E-4
26	0.0013078329187694033	2.605244858106381E-5	0.0	0.0	5.158384819050634E-4
27	0.0013390958570666798	2.605244858106381E-5	0.0	0.0	5.210489716212761E-4
28	0.001354727326215318	2.605244858106381E-5	0.0	0.0	5.418909304861272E-4
29	0.0013912007542288074	2.605244858106381E-5	0.0	0.0	5.418909304861272E-4
30	0.001422463692526084	2.605244858106381E-5	0.0	0.0	5.575223996347656E-4
31	0.0014693580999719988	2.605244858106381E-5	0.0	5.210489716212762E-6	5.679433790671911E-4
32	0.001511042017701701	2.605244858106381E-5	0.0	5.210489716212762E-6	5.835748482158293E-4
33	0.0015631469148638286	2.605244858106381E-5	0.0	5.210489716212762E-6	6.200482762293186E-4
34	0.0016517252400394455	2.605244858106381E-5	0.0	5.210489716212762E-6	6.461007248103824E-4
35	0.0017142511166339987	2.605244858106381E-5	0.0	5.210489716212762E-6	6.669426836752335E-4
36	0.0017767769932285518	3.126293829727657E-5	0.0	5.210489716212762E-6	6.929951322562973E-4
37	0.0018809867875528071	3.126293829727657E-5	0.0	5.210489716212762E-6	7.086266014049356E-4
38	0.0020216700098905516	3.126293829727657E-5	0.0	5.210489716212762E-6	7.190475808373612E-4
39	0.0020894063762013173	3.126293829727657E-5	0.0	5.210489716212762E-6	7.398895397022122E-4
40	0.002183195191093147	3.126293829727657E-5	0.0	5.210489716212762E-6	7.607314985670632E-4
41	0.0023030364545660407	3.126293829727657E-5	0.0	5.210489716212762E-6	7.867839471481271E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	44755	0.0	41.129597	12
CAACGCA	45785	0.0	40.178665	13
ATCAACG	45930	0.0	40.03647	11
ACGCAGA	45875	0.0	40.00763	15
GGTATCA	45530	0.0	39.908195	8
GTATCAA	46085	0.0	39.67745	9
AACGCAG	46835	0.0	39.287926	14
GTGGTAT	46540	0.0	39.082508	6
TGGTATC	46510	0.0	39.04709	7
CGCAGAG	47060	0.0	38.99522	16
AGTGGTA	47090	0.0	38.576134	5
AAGCAGT	47620	0.0	38.398468	1
TATCAAC	47740	0.0	38.32656	10
AGAGTAC	46645	0.0	38.319435	19
CAGAGTA	49150	0.0	36.58637	18
CAGTGGT	50250	0.0	36.337315	4
GCAGAGT	50065	0.0	36.12424	17
GAGTACA	34065	0.0	35.686337	20
GTACATG	33790	0.0	35.517757	22
AGCAGTG	51895	0.0	35.289627	2
>>END_MODULE