FastQCFastQC Report
Thu 26 May 2016
SRR805363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19800563
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4497172.2712333987675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG494600.24979087715839193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC378500.1911561807611228No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG275300.13903645063021694No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG250790.12665801472412677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG201300.10166377592394722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC2485900.045.76350412
ACGCAGA2489350.045.57924315
GTATCAA2483300.045.5738959
GGTATCA2474050.045.5277188
ATCAACG2497400.045.4831411
CAACGCA2500850.045.41287613
TGGTATC2482200.045.3772857
AAGCAGT2504300.045.341871
AGAGTAC2435700.045.33665519
CGCAGAG2502050.045.290616
AACGCAG2506850.045.26668514
GTGGTAT2492000.045.2242976
AGTGGTA2488450.045.2028775
TATCAAC2506100.045.15082610
CAGAGTA2467100.044.93204518
GCAGAGT2486850.044.78026217
GTACATG1706400.044.7028822
CAGTGGT2525400.044.65134
GAGTACA1735800.044.58745220
ACATGGG1715700.044.49481224