##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805363_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19800563
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.761381077901675	37.0	35.0	37.0	35.0	37.0
2	35.73582286523873	37.0	35.0	37.0	35.0	37.0
3	37.38613083880494	39.0	37.0	39.0	35.0	39.0
4	37.348231916435914	39.0	37.0	39.0	34.0	39.0
5	37.28732748659723	39.0	37.0	39.0	34.0	39.0
6	37.308047604504985	39.0	37.0	39.0	34.0	39.0
7	37.08532878585321	39.0	37.0	39.0	33.0	39.0
8	37.980286621143044	40.0	38.0	40.0	34.0	40.0
9	37.99467863615797	40.0	38.0	40.0	33.0	40.0
10	37.63464508559681	40.0	37.0	40.0	33.0	40.0
11	37.790599388512334	40.0	37.0	40.0	33.0	40.0
12	37.83971784034625	40.0	38.0	40.0	33.0	40.0
13	37.95487072766567	40.0	38.0	40.0	34.0	40.0
14	37.8710535654971	40.0	38.0	40.0	33.0	40.0
15	37.83018346498531	40.0	38.0	40.0	33.0	40.0
16	37.706570818213606	40.0	37.0	40.0	33.0	40.0
17	37.607329953193755	40.0	37.0	40.0	33.0	40.0
18	37.54342565915929	40.0	37.0	40.0	32.0	40.0
19	37.42343351550156	40.0	37.0	40.0	32.0	40.0
20	37.3412678720297	40.0	37.0	40.0	32.0	40.0
21	37.11275093541532	39.0	37.0	40.0	32.0	40.0
22	37.18155913041463	39.0	37.0	40.0	32.0	40.0
23	36.648010715654905	39.0	36.0	40.0	30.0	40.0
24	36.83493550158145	39.0	36.0	40.0	31.0	40.0
25	36.864780208522355	39.0	36.0	40.0	31.0	40.0
26	36.76569661175796	39.0	36.0	40.0	30.0	40.0
27	36.77840640187857	39.0	36.0	40.0	30.0	40.0
28	36.646060771100295	39.0	36.0	40.0	30.0	40.0
29	36.56223542734618	39.0	36.0	40.0	30.0	40.0
30	36.466550824842706	39.0	36.0	40.0	30.0	40.0
31	36.27894727033772	39.0	35.0	40.0	30.0	40.0
32	36.14494067668682	38.0	35.0	40.0	30.0	40.0
33	35.99278242744916	38.0	35.0	40.0	29.0	40.0
34	35.86274875113399	38.0	35.0	40.0	29.0	40.0
35	35.68195515450748	38.0	35.0	40.0	27.0	40.0
36	35.646163697466584	38.0	34.0	40.0	28.0	40.0
37	35.50966444742001	38.0	34.0	40.0	27.0	40.0
38	35.58664549083781	38.0	34.0	40.0	28.0	40.0
39	35.64802894745973	38.0	34.0	40.0	28.0	40.0
40	35.61519205287244	38.0	34.0	40.0	28.0	40.0
41	35.60173112249384	38.0	34.0	40.0	28.0	40.0
42	35.51386771174133	38.0	34.0	40.0	28.0	40.0
43	35.467870938821285	38.0	34.0	40.0	28.0	40.0
44	35.34295883404931	38.0	34.0	40.0	27.0	40.0
45	35.22207116029983	38.0	34.0	40.0	27.0	40.0
46	35.074054863995535	38.0	34.0	40.0	27.0	40.0
47	34.952135855935005	37.0	33.0	40.0	26.0	40.0
48	34.87816826218527	37.0	33.0	40.0	26.0	40.0
49	34.66754359459375	37.0	33.0	40.0	26.0	40.0
50	34.495168091937586	37.0	33.0	40.0	26.0	40.0
51	34.21597471748657	37.0	33.0	39.0	25.0	40.0
52	33.93386511282533	36.0	32.0	39.0	24.0	40.0
53	33.672290277806745	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	8.0
13	60.0
14	144.0
15	416.0
16	1161.0
17	2701.0
18	6222.0
19	11946.0
20	21278.0
21	34162.0
22	51058.0
23	72466.0
24	98803.0
25	130831.0
26	168811.0
27	212774.0
28	267107.0
29	328964.0
30	406996.0
31	502668.0
32	625807.0
33	795303.0
34	1039590.0
35	1416487.0
36	2017174.0
37	3006960.0
38	4288191.0
39	4292470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.402264066935874	35.60143214109619	28.32046240301349	20.675841388954446
2	24.704580369760194	38.19480284474739	18.190932247734573	18.90968453775784
3	35.45036572949971	23.26349508344788	19.94854893772465	21.33759024932776
4	21.742639338083467	22.483668772448542	20.71994619546929	35.0537456939987
5	21.882190925581256	35.04628631014179	22.04477721163787	21.026745552639085
6	34.31735249144178	22.708980547674326	21.11578342494605	21.85788353593784
7	22.096977747551925	22.363288357002777	34.588016512459774	20.951717382985525
8	33.35312233293568	23.309928106589698	22.405261910987075	20.931687649487543
9	33.357142420647335	22.934352927237473	22.541485310291428	21.167019341823764
10	21.095945605183044	23.21383487934156	34.739173830562294	20.9510456849131
11	20.94972249021404	35.25565914464149	22.759317500214514	21.03530086492995
12	20.820796863200304	23.01650210653101	34.9812275539842	21.181473476284488
13	20.84376590706032	23.057581746539228	22.94253451278128	33.15611783361918
14	21.098799059400484	35.2229681549964	22.669309958509764	21.00892282709335
15	20.918278939846306	35.14963185642752	22.681703545500195	21.25038565822598
16	21.007857200828077	22.86660737879019	22.83074476215651	33.29479065822522
17	33.33508244184774	22.72773253972627	22.720465069604334	21.21671994882166
18	21.148211795795905	22.72703558984661	22.7194145944234	33.40533801993408
19	21.22909838472775	34.917320280236474	22.762418422142844	21.09116291289293
20	33.514531884775195	22.84074952818261	22.671006879955886	20.97371170708631
21	21.34978182186032	34.84650916239099	22.653956859711514	21.14975215603718
22	33.35527883727347	22.721818566472074	22.865698313729766	21.057204282524694
23	21.490388934900487	22.706268503577398	34.704018264531165	21.099324296990947
24	21.296960091488305	34.73205282092231	22.681799502367685	21.28918758522169
25	21.13309101362421	22.8947782949404	22.683981258512702	33.288149432922694
26	21.380725386444823	31.146488107434116	26.26595516501223	21.20683134110884
27	21.47568733272887	22.754716620936485	34.57488052233666	21.194715523997978
28	29.9441637088804	22.595685789338415	26.364326105272866	21.095824396508323
29	30.05485753107121	22.748671338284673	26.067980996297933	21.12849013434618
30	29.89485197971391	22.74688351033251	26.072511170515707	21.285753339437875
31	26.965975664429344	23.886184448391695	26.944693441292557	22.20314644588641
32	24.929205295829217	25.008273754640207	27.257214857981566	22.805306091549014
33	24.445299863443275	24.414068428256307	27.889025175698283	23.25160653260213
34	23.603611675082167	24.47515254995527	28.277327265896428	23.64390850906613
35	23.671650144493366	24.594952173834653	28.403990330982005	23.32940735068998
36	23.58896562688647	24.55561490852558	28.5801519886076	23.275267475980353
37	24.074290210838956	24.241906656896575	28.46320076858421	23.220602363680264
38	24.20575111929898	24.411694758376314	28.197061871422548	23.185492250902158
39	24.351782320533008	24.39981630825346	28.0480711583807	23.20033021283284
40	23.739087620892395	24.816945861589897	27.991704074273038	23.45226244324467
41	24.11865763614903	25.05906523971061	27.780644419050105	23.041632705090255
42	24.06554298481311	25.089417912005835	27.830829860746885	23.014209242434166
43	23.93005188791854	24.82602136110978	28.314371667108656	22.929555083863022
44	24.267698852805346	24.559655197683014	27.88545962051685	23.287186328994785
45	24.175287339051927	24.29008710509898	27.977295393065337	23.557330162783753
46	23.79226287656568	24.474647513810595	27.817375697852633	23.915713911771093
47	23.89968911490042	24.531292367797825	27.84905661520837	23.719961902093388
48	23.905022296588232	24.419315753799527	27.843672929906084	23.831989019706157
49	23.476382969514553	25.26600379999296	27.629911331309113	23.62770189918337
50	24.176671138088345	24.889140778471806	27.579175400214627	23.35501268322522
51	23.923052086953287	24.72589289506566	27.780851483869423	23.57020353411163
52	24.285652887748697	24.574493159613695	27.606543308894803	23.53331064374281
53	24.813905543998928	24.68082346951448	26.93809766924304	23.567173317243554
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5658.0
1	3302.0
2	946.0
3	860.5
4	775.0
5	814.0
6	853.0
7	1035.0
8	1572.0
9	1927.0
10	2604.0
11	3281.0
12	4453.0
13	5625.0
14	7534.5
15	9444.0
16	13840.5
17	18237.0
18	24788.5
19	31340.0
20	46400.0
21	61460.0
22	325869.0
23	590278.0
24	407850.5
25	225425.0
26	225427.0
27	244593.5
28	263760.0
29	286482.0
30	309204.0
31	331643.5
32	354083.0
33	396378.0
34	438673.0
35	495583.0
36	552493.0
37	610619.5
38	668746.0
39	728812.0
40	788878.0
41	851186.5
42	976569.5
43	1039644.0
44	1121492.5
45	1203341.0
46	1255457.0
47	1307573.0
48	1378829.0
49	1450085.0
50	1471764.0
51	1493443.0
52	1491156.0
53	1488869.0
54	1456408.5
55	1423948.0
56	1373412.5
57	1322877.0
58	1198483.5
59	954853.0
60	835616.0
61	722389.5
62	609163.0
63	520252.0
64	431341.0
65	354757.0
66	278173.0
67	224152.0
68	170131.0
69	134796.0
70	99461.0
71	75833.5
72	52206.0
73	38651.5
74	25097.0
75	18839.5
76	9423.5
77	6265.0
78	4556.5
79	2848.0
80	2158.5
81	1469.0
82	1100.5
83	732.0
84	483.5
85	235.0
86	164.0
87	93.0
88	63.5
89	34.0
90	21.5
91	9.0
92	8.5
93	5.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.9800563E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.15176392700829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.54146293475658	33.79440602123228
2	11.643705756250151	10.281802955710084
3	3.9656198847980377	5.252673389335583
4	1.9531834341104184	3.449459755559462
5	1.106679316911461	2.4430921971588813
6	0.7498090587593382	1.9863235531604753
7	0.5438160183959471	1.6807305524760416
8	0.4249960117339051	1.501145886399634
9	0.33692666647504393	1.3388315975027998
>10	2.4632839483595905	20.084462730695073
>50	0.17766742388831494	5.386460560246353
>100	0.08862922852353414	7.4505524118744
>500	0.003036525439348934	0.863228605926328
>1k	9.884994744149274E-4	0.8928890003821318
>5k	8.04144038582972E-5	0.2290539570582789
>10k+	1.1487771979756387E-4	3.3648868252818507
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	449717	2.2712333987675	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	49460	0.24979087715839193	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	37850	0.1911561807611228	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	27530	0.13903645063021694	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	25079	0.12665801472412677	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG	20130	0.10166377592394722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3736698799928062E-4	0.0	0.0	0.0	1.5151084340379615E-5
2	3.68676385615904E-4	0.0	0.0	5.050361446793205E-6	4.040289157434564E-5
3	3.68676385615904E-4	0.0	0.0	5.050361446793205E-6	8.585614459548448E-5
4	3.68676385615904E-4	0.0	0.0	5.050361446793205E-6	1.060575903826573E-4
5	3.7372674706269714E-4	0.0	0.0	5.050361446793205E-6	1.262590361698301E-4
6	4.040289157434564E-4	0.0	0.0	5.050361446793205E-6	1.6161156629738256E-4
7	4.141296386370428E-4	0.0	0.0	1.010072289358641E-5	1.8181301208455538E-4
8	4.141296386370428E-4	0.0	0.0	1.5151084340379615E-5	1.9696409642493498E-4
9	4.19180000083836E-4	5.050361446793205E-6	0.0	1.5151084340379615E-5	2.272662651056942E-4
10	4.2928072297742243E-4	1.010072289358641E-5	0.0	1.5151084340379615E-5	2.5756843378645344E-4
11	4.343310844242156E-4	1.010072289358641E-5	0.0	1.5151084340379615E-5	2.828202410204195E-4
12	4.4443180731780203E-4	1.010072289358641E-5	0.0	1.5151084340379615E-5	2.979713253607991E-4
13	4.4948216876459525E-4	1.5151084340379615E-5	0.0	1.5151084340379615E-5	3.080720482543855E-4
14	4.6463325310497485E-4	1.5151084340379615E-5	0.0	1.5151084340379615E-5	3.4847493982873115E-4
15	4.6968361455176807E-4	1.5151084340379615E-5	0.0	1.5151084340379615E-5	3.6362602416911075E-4
16	4.949354217857341E-4	1.5151084340379615E-5	0.0	1.5151084340379615E-5	3.7877710850949036E-4
17	5.100865061261137E-4	1.5151084340379615E-5	0.0	1.5151084340379615E-5	3.989785542966632E-4
18	5.151368675729069E-4	1.5151084340379615E-5	0.0	1.5151084340379615E-5	4.2928072297742243E-4
19	5.454390362536661E-4	1.5151084340379615E-5	0.0	1.5151084340379615E-5	4.393814458710088E-4
20	5.605901205940457E-4	1.5151084340379615E-5	0.0	2.020144578717282E-5	4.6463325310497485E-4
21	5.706908434876322E-4	1.5151084340379615E-5	0.0	2.020144578717282E-5	4.7978433744535445E-4
22	6.060433736151846E-4	2.020144578717282E-5	0.0	2.020144578717282E-5	5.100865061261137E-4
23	6.41395903742737E-4	2.020144578717282E-5	0.0	2.020144578717282E-5	5.201872290197001E-4
24	6.868491567638759E-4	2.020144578717282E-5	0.0	2.020144578717282E-5	5.353383133600797E-4
25	7.222016868914283E-4	2.020144578717282E-5	0.0	2.020144578717282E-5	5.65640482040839E-4
26	7.777556628061536E-4	2.020144578717282E-5	0.0	2.020144578717282E-5	5.757412049344254E-4
27	8.232089158272923E-4	2.020144578717282E-5	0.0	2.020144578717282E-5	5.908922892748049E-4
28	8.434103616144652E-4	2.020144578717282E-5	0.0	2.020144578717282E-5	6.060433736151846E-4
29	8.939139760823973E-4	2.020144578717282E-5	0.0	2.020144578717282E-5	6.41395903742737E-4
30	9.646190363375021E-4	2.020144578717282E-5	0.0	2.020144578717282E-5	6.767484338702894E-4
31	0.0010403744580394002	2.020144578717282E-5	0.0	2.020144578717282E-5	6.918995182106691E-4
32	0.0011161298797412982	2.020144578717282E-5	0.0	2.5251807233966025E-5	7.222016868914283E-4
33	0.0011767342171028167	2.020144578717282E-5	0.0	3.030216868075923E-5	7.474534941253943E-4
34	0.0012423889159111285	2.020144578717282E-5	0.0	3.5352530127552435E-5	7.575542170189807E-4
35	0.0013433961448469924	2.5251807233966025E-5	0.0	3.5352530127552435E-5	7.929067471465331E-4
36	0.0014242019279956837	2.5251807233966025E-5	0.0	3.5352530127552435E-5	7.979571085933264E-4
37	0.0015151084340379614	2.5251807233966025E-5	0.0	3.5352530127552435E-5	8.13108192933706E-4
38	0.001600964578633446	2.5251807233966025E-5	0.0	3.5352530127552435E-5	8.181585543804992E-4
39	0.0017423746991436556	2.5251807233966025E-5	0.0	3.5352530127552435E-5	8.181585543804992E-4
40	0.0018231804822923469	2.5251807233966025E-5	0.0	3.5352530127552435E-5	8.535110845080516E-4
41	0.001989842410036523	3.030216868075923E-5	0.0	4.5453253021138844E-5	8.585614459548449E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	248590	0.0	45.763504	12
ACGCAGA	248935	0.0	45.579243	15
GTATCAA	248330	0.0	45.573895	9
GGTATCA	247405	0.0	45.527718	8
ATCAACG	249740	0.0	45.48314	11
CAACGCA	250085	0.0	45.412876	13
TGGTATC	248220	0.0	45.377285	7
AAGCAGT	250430	0.0	45.34187	1
AGAGTAC	243570	0.0	45.336655	19
CGCAGAG	250205	0.0	45.2906	16
AACGCAG	250685	0.0	45.266685	14
GTGGTAT	249200	0.0	45.224297	6
AGTGGTA	248845	0.0	45.202877	5
TATCAAC	250610	0.0	45.150826	10
CAGAGTA	246710	0.0	44.932045	18
GCAGAGT	248685	0.0	44.780262	17
GTACATG	170640	0.0	44.70288	22
CAGTGGT	252540	0.0	44.6513	4
GAGTACA	173580	0.0	44.587452	20
ACATGGG	171570	0.0	44.494812	24
>>END_MODULE