FastQCFastQC Report
Thu 26 May 2016
SRR805362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4840555
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1976834.083891206690142No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG229990.4751314673627301No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG140340.28992543210437643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC118110.24400094617249468No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG112560.23253531878059436No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA74040.1529576670443782No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAG56990.11773443334493669No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC50810.104967302303145No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG50120.10354184592469252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT908700.045.9164161
TCAACGC909600.045.9097412
GGTATCA908000.045.8793538
GTATCAA907750.045.8686879
ACGCAGA909700.045.85044515
AGTGGTA906350.045.8487935
GTGGTAT908650.045.838776
TGGTATC909350.045.7957347
CAACGCA913150.045.7441313
ATCAACG913450.045.66736211
CGCAGAG913250.045.63361716
AACGCAG915100.045.610714
AGAGTAC892700.045.5995319
TATCAAC914550.045.5302110
AGCAGTG920400.045.3480572
CAGAGTA901150.045.31276718
GCAGAGT907400.045.28812817
CAGTGGT920000.045.2707064
GCAGTGG922750.045.227473
GTACATG577000.045.1916822