##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805362_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4840555
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.409122714234215	35.0	35.0	35.0	32.0	37.0
2	35.09839408910755	35.0	35.0	37.0	33.0	37.0
3	37.22783978283482	39.0	37.0	39.0	34.0	39.0
4	37.26921685633156	39.0	37.0	39.0	35.0	39.0
5	37.23398680523204	39.0	37.0	39.0	34.0	39.0
6	37.30684704543177	39.0	37.0	39.0	35.0	39.0
7	37.08627626377554	39.0	37.0	39.0	34.0	39.0
8	38.10704247756714	40.0	38.0	40.0	34.0	40.0
9	38.12003995409617	40.0	38.0	40.0	34.0	40.0
10	37.6067595554642	39.0	37.0	40.0	33.0	40.0
11	37.896905416837534	40.0	37.0	40.0	34.0	40.0
12	37.97570795084448	40.0	37.0	40.0	34.0	40.0
13	38.10367158311392	40.0	38.0	40.0	34.0	40.0
14	38.03328915795813	40.0	37.0	40.0	34.0	40.0
15	38.03642350928767	40.0	37.0	40.0	34.0	40.0
16	37.89095940444846	40.0	37.0	40.0	34.0	40.0
17	37.805795203235995	40.0	37.0	40.0	33.0	40.0
18	37.7067427185519	40.0	37.0	40.0	33.0	40.0
19	37.670164268353524	40.0	37.0	40.0	33.0	40.0
20	37.582730699268986	40.0	37.0	40.0	33.0	40.0
21	37.22869299078308	39.0	36.0	40.0	32.0	40.0
22	37.30087748202427	39.0	37.0	40.0	32.0	40.0
23	36.61690797852726	39.0	36.0	40.0	30.0	40.0
24	36.84937780894959	39.0	36.0	40.0	31.0	40.0
25	36.921600105772995	39.0	36.0	40.0	31.0	40.0
26	36.85203122369232	39.0	36.0	40.0	31.0	40.0
27	36.843239876419126	39.0	36.0	40.0	31.0	40.0
28	36.80237493427923	39.0	36.0	40.0	31.0	40.0
29	36.644537041723524	39.0	36.0	40.0	30.0	40.0
30	36.55107358557025	39.0	36.0	40.0	30.0	40.0
31	36.341392051118106	39.0	35.0	40.0	30.0	40.0
32	36.18648791305956	38.0	35.0	40.0	30.0	40.0
33	35.98761712241675	38.0	35.0	40.0	30.0	40.0
34	35.843992476069374	38.0	35.0	40.0	29.0	40.0
35	35.800559646569454	38.0	35.0	40.0	29.0	40.0
36	35.6219061657186	38.0	34.0	40.0	28.0	40.0
37	35.44905016057043	38.0	34.0	40.0	28.0	40.0
38	35.200698473625444	38.0	33.0	40.0	27.0	40.0
39	35.150932899223335	38.0	33.0	40.0	27.0	40.0
40	35.05600845357609	37.0	33.0	40.0	27.0	40.0
41	34.84554126541275	37.0	33.0	40.0	26.0	40.0
42	34.73587181635164	37.0	33.0	40.0	26.0	40.0
43	34.570577134233574	37.0	33.0	40.0	26.0	40.0
44	34.52048308510078	37.0	33.0	40.0	26.0	40.0
45	34.308568129067844	37.0	33.0	40.0	25.0	40.0
46	34.47125236672241	37.0	33.0	40.0	26.0	40.0
47	34.42391399333341	37.0	33.0	40.0	26.0	40.0
48	34.43758659905734	37.0	33.0	40.0	26.0	40.0
49	34.340549379151774	37.0	33.0	40.0	26.0	40.0
50	34.09487693043463	36.0	33.0	39.0	25.0	40.0
51	33.76398160954684	36.0	32.0	39.0	23.0	40.0
52	33.428364102876635	36.0	32.0	39.0	23.0	40.0
53	33.14645841231016	36.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	11.0
14	21.0
15	41.0
16	131.0
17	344.0
18	678.0
19	1481.0
20	2874.0
21	5166.0
22	8242.0
23	12819.0
24	18991.0
25	26787.0
26	36055.0
27	47646.0
28	62022.0
29	79837.0
30	105055.0
31	135906.0
32	178054.0
33	233678.0
34	311108.0
35	418596.0
36	573035.0
37	787542.0
38	982629.0
39	811804.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.825935249160478	48.52146086554124	20.18406980191321	17.468534083385066
2	23.00606025548723	47.28057009991623	15.584246021375648	14.12912362322089
3	40.81073347994187	22.218733182455317	19.030792956592787	17.939740381010026
4	19.512824459178752	22.19600851555245	19.12669105092288	39.16447597434592
5	18.091210615311674	41.13460130088389	21.684166381747545	19.09002170205689
6	37.38573366070626	21.411139838303665	20.852443573102672	20.3506829278874
7	20.20518308334478	21.56283731927434	39.088926786287935	19.143052811092943
8	36.8345778531594	22.258873207721017	21.676729218033884	19.229819721085704
9	37.40137236329305	21.709783278983505	21.66007823483051	19.22876612289293
10	19.147308521440205	22.328596617536626	39.57816820591854	18.945926655104632
11	18.754936159180094	40.53663267951712	21.59023087228634	19.118200289016446
12	18.79999297601205	21.972335816863975	39.905609997200735	19.32206120992324
13	18.880727519881503	22.311883657968973	21.486089921506935	37.32129890064259
14	19.008626076968447	40.57394245081401	21.380647467077637	19.036784005139907
15	18.88483861871211	40.31519112994275	21.396389463604898	19.40358078774025
16	19.058289803545257	22.034415475084984	21.432067190642396	37.475227530727366
17	37.34317655723362	21.828261428699808	21.519970333980297	19.30859168008627
18	19.07382521219158	21.69224396789211	21.4432022774248	37.790728542491514
19	19.224200530724268	40.32364057427299	21.24386563111048	19.20829326389226
20	37.64801763434152	21.961159412505385	21.400913738197378	18.989909214955723
21	19.591864982424536	39.936019733274385	21.275019083555502	19.197096200745577
22	37.619012695858224	21.85108938954314	21.406780834015933	19.123117080582702
23	19.578085570766163	21.835326734227788	39.507783714883935	19.078803980122114
24	19.508258867010085	39.6314678791998	21.3785402706921	19.481732983098013
25	19.2830161004265	22.021875590712224	21.253926460912023	37.44118184794926
26	19.434775557761455	33.54340566319358	27.776091791127257	19.245726987917706
27	19.69999307930599	21.578393386708754	39.34015830829316	19.381455225692097
28	31.33589020267304	21.692305944256393	27.697939595769494	19.273864257301074
29	31.4398452243596	21.758000890393767	27.58497734247416	19.21717654277247
30	31.327461417130888	21.568353215695307	27.63794647514593	19.466238892027874
31	27.43695712578413	23.375501362963544	28.64512437106902	20.542417140183307
32	24.705286893754952	24.737163403783242	29.22447942436353	21.333070278098276
33	24.215900862607697	23.67259539453637	29.89450589860047	22.216997844255463
34	22.917785253963647	23.997082979121195	30.18814991256168	22.89698185435348
35	22.418999474233843	24.032120283727796	31.111763010646502	22.437117231391852
36	23.01839355197906	23.81956201303363	31.063380128931495	22.098664306055817
37	23.85676849039005	23.73607985034774	30.518504592965062	21.88864706629715
38	23.612313050879497	24.03484724375614	30.592710959796964	21.760128745567396
39	23.930954198433856	23.846149873309983	30.40203034569383	21.82086558256233
40	23.073098022850687	24.856488563811382	29.893741523441008	22.176671889896923
41	23.490384883551577	25.172030893151714	29.77844482709111	21.5591393962056
42	23.47960099616676	25.007958798113027	29.945553763979543	21.566886441740667
43	23.13369024832896	24.78947145523602	30.610229612100266	21.46660868433475
44	23.638405100241606	24.18995342476224	30.18003100884093	21.991610466155223
45	23.92919820144591	23.667554650241556	30.094544943709966	22.30870220460257
46	23.149432244856218	24.015180077491113	29.617864067240223	23.217523610412442
47	22.697665866827254	24.11797820704444	30.245064873759308	22.939291052368997
48	22.72848877866278	23.788367243012424	30.140676017522782	23.342467960802015
49	22.240486886317786	25.486147766113596	29.469410016000232	22.803955331568385
50	23.544407614416116	24.778708226639303	29.500811373902376	22.176072785042212
51	23.2485531101289	24.304134546555094	29.91813955217945	22.529172791136553
52	23.86013587284929	24.38619125286253	29.305461873690103	22.44821100059807
53	24.66219266179188	24.410300058567664	28.55311012890051	22.37439715073995
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2574.0
1	1528.0
2	482.0
3	438.5
4	395.0
5	418.5
6	442.0
7	494.5
8	669.5
9	792.0
10	1036.0
11	1280.0
12	1664.0
13	2048.0
14	2683.0
15	3318.0
16	4681.5
17	6045.0
18	8260.5
19	10476.0
20	14658.5
21	18841.0
22	131902.0
23	244963.0
24	161317.5
25	75765.5
26	73859.0
27	78421.5
28	82984.0
29	86779.0
30	90574.0
31	94463.5
32	98353.0
33	108014.5
34	117676.0
35	131967.0
36	146258.0
37	160190.0
38	174122.0
39	188341.0
40	202560.0
41	215761.5
42	239662.5
43	250362.0
44	268394.5
45	286427.0
46	296288.0
47	306149.0
48	320671.0
49	335193.0
50	337429.0
51	339665.0
52	334439.0
53	329213.0
54	323992.5
55	318772.0
56	318312.5
57	317853.0
58	280974.0
59	210348.5
60	176602.0
61	152276.5
62	127951.0
63	108856.0
64	89761.0
65	73027.0
66	56293.0
67	45248.0
68	34203.0
69	27181.0
70	20159.0
71	15349.5
72	10540.0
73	7958.5
74	5377.0
75	4073.0
76	2219.0
77	1669.0
78	1361.5
79	1054.0
80	873.5
81	693.0
82	511.5
83	330.0
84	223.5
85	117.0
86	82.0
87	47.0
88	36.5
89	26.0
90	16.5
91	7.0
92	5.5
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	4840555.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.504026483910316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.60700830320681	43.12799507043555
2	9.76436923624086	10.448661404290203
3	3.3874426033521234	5.437254562874344
4	1.8509763737889238	3.9613875569717947
5	1.1255999593851647	3.011206501861612
6	0.7066175458295249	2.2684130331635166
7	0.48001658763165794	1.7977974152152336
8	0.3427564804281148	1.4671081445086194
9	0.24236475658566062	1.1670741319613096
>10	1.366955237740222	13.666777291674476
>50	0.09662976967989396	3.4871879406434054
>100	0.026172445462107518	2.307166189039503
>500	0.0014661019145607475	0.547421585302111
>1k	0.0012679795156313608	1.3476179185297987
>5k	1.5849743945389286E-4	0.49176976698205555
>10k+	1.981217993173661E-4	5.465161486546612
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	197683	4.083891206690142	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	22999	0.4751314673627301	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	14034	0.28992543210437643	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	11811	0.24400094617249468	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	11256	0.23253531878059436	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	7404	0.1529576670443782	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAG	5699	0.11773443334493669	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	5081	0.104967302303145	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG	5012	0.10354184592469252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.263515237405628E-5	0.0	0.0	0.0	0.0
2	8.263515237405628E-5	0.0	0.0	0.0	0.0
3	1.0329394046757035E-4	0.0	0.0	0.0	2.065878809351407E-5
4	1.239527285610844E-4	0.0	0.0	2.065878809351407E-5	2.065878809351407E-5
5	1.239527285610844E-4	0.0	0.0	2.065878809351407E-5	2.065878809351407E-5
6	1.239527285610844E-4	0.0	0.0	2.065878809351407E-5	2.065878809351407E-5
7	1.239527285610844E-4	0.0	0.0	2.065878809351407E-5	2.065878809351407E-5
8	1.239527285610844E-4	0.0	0.0	2.065878809351407E-5	2.065878809351407E-5
9	1.239527285610844E-4	0.0	0.0	4.131757618702814E-5	2.065878809351407E-5
10	1.239527285610844E-4	0.0	0.0	4.131757618702814E-5	2.065878809351407E-5
11	1.239527285610844E-4	0.0	0.0	4.131757618702814E-5	2.065878809351407E-5
12	1.239527285610844E-4	0.0	0.0	6.19763642805422E-5	2.065878809351407E-5
13	1.239527285610844E-4	0.0	0.0	6.19763642805422E-5	8.263515237405628E-5
14	1.239527285610844E-4	0.0	0.0	6.19763642805422E-5	1.0329394046757035E-4
15	1.239527285610844E-4	0.0	0.0	6.19763642805422E-5	1.239527285610844E-4
16	1.239527285610844E-4	0.0	0.0	6.19763642805422E-5	1.8592909284162663E-4
17	1.239527285610844E-4	0.0	0.0	6.19763642805422E-5	2.065878809351407E-4
18	2.065878809351407E-4	0.0	0.0	6.19763642805422E-5	2.479054571221688E-4
19	2.479054571221688E-4	0.0	0.0	6.19763642805422E-5	2.479054571221688E-4
20	4.958109142443376E-4	0.0	0.0	6.19763642805422E-5	2.479054571221688E-4
21	5.991048547119081E-4	0.0	0.0	6.19763642805422E-5	2.6856424521568294E-4
22	5.991048547119081E-4	0.0	0.0	6.19763642805422E-5	2.89223033309197E-4
23	6.610812189924503E-4	0.0	0.0	8.263515237405628E-5	3.7185818568325325E-4
24	0.001136233345143274	0.0	0.0	8.263515237405628E-5	3.7185818568325325E-4
25	0.0012808448617978724	0.0	0.0	8.263515237405628E-5	3.925169737767673E-4
26	0.0013015036498913864	0.0	0.0	8.263515237405628E-5	4.131757618702814E-4
27	0.0013221624379849005	0.0	0.0	1.0329394046757035E-4	4.131757618702814E-4
28	0.0014254563784524708	0.0	0.0	1.0329394046757035E-4	4.338345499637955E-4
29	0.001487432742733013	0.0	0.0	1.0329394046757035E-4	4.5449333805730956E-4
30	0.0015287503189200411	0.0	0.0	1.0329394046757035E-4	4.958109142443376E-4
31	0.0022104903260060055	0.0	0.0	1.0329394046757035E-4	4.958109142443376E-4
32	0.0025410309355022305	0.0	0.0	1.0329394046757035E-4	5.164697023378518E-4
33	0.0033673824592427935	0.0	0.0	1.0329394046757035E-4	5.164697023378518E-4
34	0.0036566054925519904	0.0	0.0	1.0329394046757035E-4	5.164697023378518E-4
35	0.004214392771076871	0.0	0.0	1.0329394046757035E-4	5.577872785248799E-4
36	0.004813497625788778	0.0	0.0	1.239527285610844E-4	5.991048547119081E-4
37	0.0057844606661839396	0.0	0.0	1.446115166545985E-4	5.991048547119081E-4
38	0.006941352799420728	0.0	0.0	1.446115166545985E-4	5.991048547119081E-4
39	0.007912315839815888	0.0	0.0	1.446115166545985E-4	6.404224308989362E-4
40	0.008738667363556451	0.0	0.0	1.6527030474811256E-4	6.817400070859643E-4
41	0.010122806165821894	0.0	0.0	1.6527030474811256E-4	6.817400070859643E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	90870	0.0	45.916416	1
TCAACGC	90960	0.0	45.90974	12
GGTATCA	90800	0.0	45.879353	8
GTATCAA	90775	0.0	45.868687	9
ACGCAGA	90970	0.0	45.850445	15
AGTGGTA	90635	0.0	45.848793	5
GTGGTAT	90865	0.0	45.83877	6
TGGTATC	90935	0.0	45.795734	7
CAACGCA	91315	0.0	45.74413	13
ATCAACG	91345	0.0	45.667362	11
CGCAGAG	91325	0.0	45.633617	16
AACGCAG	91510	0.0	45.6107	14
AGAGTAC	89270	0.0	45.59953	19
TATCAAC	91455	0.0	45.53021	10
AGCAGTG	92040	0.0	45.348057	2
CAGAGTA	90115	0.0	45.312767	18
GCAGAGT	90740	0.0	45.288128	17
CAGTGGT	92000	0.0	45.270706	4
GCAGTGG	92275	0.0	45.22747	3
GTACATG	57700	0.0	45.19168	22
>>END_MODULE