FastQCFastQC Report
Thu 26 May 2016
SRR805361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17940374
Sequences flagged as poor quality0
Sequence length53
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1615720.9006055280675865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC619700.040.40543419
AAGCAGT638800.039.973541
GGTATCA646650.039.3901638
TCAACGC653750.039.343412
TGGTATC645700.039.3316547
GTATCAA649350.039.222769
ATCAACG656800.039.13923311
ACGCAGA655000.039.08534215
GAGTACT295850.038.76288620
CGCAGAG663950.038.53015516
GTACATG353500.037.79943522
CAGAGTA669900.037.65472418
GAGTACA362000.037.41823220
GCAGAGT678800.037.20602417
AGTACTT308800.037.20579521
GTACTTT307950.037.16349822
ACATGGG362550.036.69383224
TACATGG364700.036.53550723
GTGGTAT696350.036.5349276
AGTGGTA698350.036.3932845
Produced by FastQC (version 0.11.5)