##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805361_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17940374
Sequences flagged as poor quality	0
Sequence length	53
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.66931809782784	37.0	35.0	37.0	35.0	37.0
2	35.689277826649544	37.0	35.0	37.0	35.0	37.0
3	37.295821592125115	39.0	37.0	39.0	34.0	39.0
4	37.319293510826476	39.0	37.0	39.0	34.0	39.0
5	37.29359627619803	39.0	37.0	39.0	34.0	39.0
6	37.224960527578745	39.0	37.0	39.0	34.0	39.0
7	37.122261219303454	39.0	37.0	39.0	33.0	39.0
8	38.03355359258397	40.0	38.0	40.0	34.0	40.0
9	37.59154117968778	40.0	37.0	40.0	33.0	40.0
10	37.74034426484086	40.0	37.0	40.0	33.0	40.0
11	37.87873842540852	40.0	37.0	40.0	33.0	40.0
12	37.812477933849095	40.0	37.0	40.0	33.0	40.0
13	37.93463948967842	40.0	38.0	40.0	34.0	40.0
14	37.81293282960545	40.0	37.0	40.0	33.0	40.0
15	37.79492032886271	40.0	37.0	40.0	33.0	40.0
16	37.702278057302486	40.0	37.0	40.0	33.0	40.0
17	37.58497136124364	40.0	37.0	40.0	33.0	40.0
18	37.49153668702782	40.0	37.0	40.0	32.0	40.0
19	37.4895851112134	40.0	37.0	40.0	32.0	40.0
20	37.29476698757785	40.0	37.0	40.0	32.0	40.0
21	37.20797342351949	39.0	37.0	40.0	32.0	40.0
22	37.131800318098165	39.0	37.0	40.0	32.0	40.0
23	36.944339956346504	39.0	36.0	40.0	31.0	40.0
24	36.928318997140195	39.0	36.0	40.0	31.0	40.0
25	36.83655452221899	39.0	36.0	40.0	31.0	40.0
26	36.61589975771965	39.0	36.0	40.0	30.0	40.0
27	36.5337449486839	39.0	36.0	40.0	30.0	40.0
28	36.43910701081259	39.0	36.0	40.0	30.0	40.0
29	36.38422727419172	39.0	35.0	40.0	30.0	40.0
30	36.16876337137676	38.0	35.0	40.0	30.0	40.0
31	36.10659772198729	38.0	35.0	40.0	30.0	40.0
32	35.943655466714354	38.0	35.0	40.0	29.0	40.0
33	35.80593219517051	38.0	35.0	40.0	28.0	40.0
34	35.69900666507844	38.0	34.0	40.0	28.0	40.0
35	35.64812060216805	38.0	34.0	40.0	28.0	40.0
36	35.484104289018724	38.0	34.0	40.0	27.0	40.0
37	35.32519985369313	38.0	34.0	40.0	27.0	40.0
38	35.2994190087676	38.0	34.0	40.0	27.0	40.0
39	35.251003741616536	38.0	34.0	40.0	27.0	40.0
40	35.16516099385665	38.0	33.0	40.0	27.0	40.0
41	34.98261178947551	38.0	33.0	40.0	26.0	40.0
42	34.847849604473126	37.0	33.0	40.0	26.0	40.0
43	34.6682513419174	37.0	33.0	40.0	26.0	40.0
44	34.55285909870106	37.0	33.0	40.0	26.0	40.0
45	34.2762898365441	37.0	33.0	39.0	25.0	40.0
46	34.34728088723234	37.0	33.0	39.0	25.0	40.0
47	34.38951623862468	37.0	33.0	39.0	26.0	40.0
48	34.38621218264458	37.0	33.0	39.0	26.0	40.0
49	34.262404897467576	36.0	33.0	39.0	26.0	40.0
50	34.04409122128669	36.0	32.0	39.0	25.0	40.0
51	33.77453374160427	36.0	32.0	39.0	24.0	40.0
52	33.52888741338391	35.0	32.0	39.0	23.0	40.0
53	33.37338669751255	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	10.0
12	45.0
13	135.0
14	330.0
15	885.0
16	1937.0
17	4159.0
18	8090.0
19	14597.0
20	23853.0
21	36696.0
22	52109.0
23	72311.0
24	96725.0
25	125522.0
26	160403.0
27	203108.0
28	253683.0
29	316247.0
30	394078.0
31	490124.0
32	615987.0
33	784113.0
34	1019103.0
35	1374274.0
36	1922988.0
37	2810125.0
38	3877755.0
39	3280980.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.542699054099987	28.880055677769036	21.856578909670446	24.72066635846053
2	25.31749338113018	25.867554377628917	26.147147211089354	22.667805030151545
3	24.665723245234464	24.510169074513165	25.33220321939777	25.49190446085461
4	23.531861710352302	25.644025035375517	25.139314264017017	25.684798990255164
5	25.221107430647766	28.28844593763764	23.957884044111903	22.53256258760269
6	26.72511732475588	24.437885185671156	25.2400702460272	23.59692724354576
7	24.075178142885985	25.187590849555306	27.731339380104338	23.005891627454368
8	26.330889199968745	24.479606723917797	25.3476265322005	23.841877543912965
9	26.414059149491532	24.48513615156518	25.657731550078054	23.443073148865235
10	23.300121836924916	25.16426357666791	28.301996379785617	23.233618206621557
11	23.228467812320968	27.72909305012259	25.44178844878039	23.600650688776053
12	23.204198530086384	24.77868633061942	28.224824075573903	23.7922910637203
13	23.472375770984485	25.018285571973024	24.988826877299214	26.520511779743277
14	23.488417799985665	27.753590867169216	25.1055524260531	23.65243890679202
15	23.5366386453259	27.672717413806424	25.004528891092235	23.78611504977544
16	23.814693049319928	24.84165603236588	24.846499855577147	26.49715106273704
17	26.606747440159275	24.759790403477652	25.074995649477543	23.55846650688553
18	23.514504212676947	24.794338178234188	24.95556112709802	26.735596481990843
19	24.003970039866505	27.62566711262541	24.704758105934694	23.665604741573393
20	26.78385634546972	24.639491908028226	24.917858457131384	23.658793289370667
21	23.885611303309506	27.555300686596613	24.73529258643103	23.82379542366285
22	27.041599021291308	24.541528509940765	24.732070802983262	23.684801665784672
23	23.75273781917813	24.631727298438705	27.96475703349328	23.650777848889884
24	23.83405162010558	27.525992490457558	24.607909511808394	24.032046377628472
25	23.94830230406568	24.535480698451437	24.669379802227088	26.846837195255794
26	23.85272458645511	26.05012582234908	26.209793619687083	23.887355971508732
27	24.1012924256763	24.519299318955113	27.55649352683506	23.822914728533533
28	25.725801479946853	24.449874902273496	26.01404519214594	23.81027842563371
29	25.66955404608622	24.60338340772606	25.98338808321387	23.74367446297385
30	25.500254342523743	24.46560478616555	26.006525839427873	24.027615031882835
31	25.156075341573146	24.80585967717284	25.938717888489947	24.099347092764063
32	24.68369388508846	25.12937578670322	26.156305325630335	24.030625002577985
33	24.53060900514114	24.843172165753067	26.295299083508517	24.330919745597278
34	24.398616216139086	24.91013843970031	26.355531941530312	24.33571340263029
35	24.35307647432545	25.312225932413675	26.26543348538888	24.069264107871998
36	24.167639983425097	25.047978375478685	26.393178871298893	24.391202769797328
37	24.334933039857475	25.0010507027334	26.341334913084868	24.32268134432426
38	24.35334960129594	25.155233664582465	26.254826125698383	24.236590608423214
39	24.71922826135063	24.886794444753495	26.031999109940518	24.36197818395536
40	24.608734466739655	24.878483581223	26.117459981603503	24.39532197043384
41	24.44101221078223	25.214836658366206	26.188305773335607	24.155845357515958
42	24.672590437635247	24.993425443639023	26.119249241961178	24.214734876764552
43	24.6076308108181	25.099354116029023	25.989218507930772	24.303796565222108
44	24.61166082713772	25.003263588596315	26.095280956796106	24.289794627469863
45	24.542944310971443	24.96248405969686	26.155502666778297	24.3390689625534
46	24.361588002568954	25.30618926896396	26.01779093345546	24.314431795011632
47	24.377964472758485	25.20729500956892	26.19744159179736	24.217298925875237
48	24.675076450468648	24.949775294539567	26.238143084419534	24.13700517057225
49	24.47890439742226	25.2239111626101	25.97919084630008	24.317993593667556
50	24.52615536331628	25.2180807378932	26.063336249288895	24.19242764950162
51	24.434691272322418	25.126148429235645	26.0566529995417	24.382507298900236
52	24.60720718531286	25.201302938277653	25.826406963422276	24.36508291298721
53	24.515224710477053	25.311891491225325	25.815348108127512	24.357535690170113
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18708.0
1	11300.5
2	3893.0
3	3410.0
4	2927.0
5	2976.5
6	3026.0
7	3359.0
8	4229.0
9	4766.0
10	5922.5
11	7079.0
12	9220.0
13	11361.0
14	14036.0
15	16711.0
16	22054.5
17	27398.0
18	35021.0
19	42644.0
20	55546.5
21	68449.0
22	176021.5
23	283594.0
24	221789.5
25	169446.0
26	178907.0
27	197356.0
28	215805.0
29	236015.0
30	256225.0
31	275632.5
32	295040.0
33	335850.5
34	376661.0
35	416400.0
36	456139.0
37	500339.0
38	544539.0
39	597905.5
40	651272.0
41	695542.0
42	805171.5
43	870531.0
44	963113.5
45	1055696.0
46	1106304.5
47	1156913.0
48	1286609.5
49	1416306.0
50	1470343.0
51	1524380.0
52	1492619.0
53	1460858.0
54	1374571.0
55	1288284.0
56	1196668.0
57	1105052.0
58	1041565.5
59	929860.5
60	881642.0
61	759287.5
62	636933.0
63	544570.5
64	452208.0
65	376458.5
66	300709.0
67	246997.5
68	193286.0
69	155047.5
70	116809.0
71	94558.0
72	72307.0
73	50634.5
74	28962.0
75	20988.5
76	10360.0
77	7705.0
78	6169.0
79	4633.0
80	4238.5
81	3844.0
82	3051.5
83	2259.0
84	1496.5
85	734.0
86	509.0
87	284.0
88	212.5
89	141.0
90	121.5
91	102.0
92	76.0
93	32.0
94	14.0
95	9.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.7940374E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.54565788184242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.72436101155968	20.705842974691144
2	18.039646269910303	12.103036041597317
3	8.029593773361025	8.080740169540233
4	3.9505828889940515	5.3009960811222046
5	2.0981083826175615	3.519121300115723
6	1.296981635729021	2.6104861338718863
7	0.8502233977429816	1.9964912256676588
8	0.6069845671779351	1.628935730408737
9	0.4554052430890629	1.3749181634036691
>10	2.735113588889728	16.772677041892187
>50	0.11042903368171025	2.4557626944549367
>100	0.05699138602747243	4.179158569557394
>500	0.02227561806066136	5.560806516814933
>1k	0.02314878039135308	12.330776785254423
>5k	8.57904262529946E-5	0.19953030548262884
>10k+	6.863234100239569E-5	1.1807202661248646
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	161572	0.9006055280675865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.678853071847889E-4	0.0	0.0	0.0	0.0
2	3.678853071847889E-4	0.0	0.0	0.0	5.574019805830135E-6
3	3.678853071847889E-4	0.0	0.0	0.0	5.574019805830135E-6
4	3.678853071847889E-4	0.0	0.0	0.0	1.6722059417490405E-5
5	3.678853071847889E-4	5.574019805830135E-6	0.0	0.0	2.7870099029150673E-5
6	3.7903334679644917E-4	1.114803961166027E-5	0.0	0.0	3.901813864081095E-5
7	3.7903334679644917E-4	1.114803961166027E-5	0.0	0.0	3.901813864081095E-5
8	3.7903334679644917E-4	1.114803961166027E-5	0.0	0.0	4.459215844664108E-5
9	3.7903334679644917E-4	1.114803961166027E-5	0.0	0.0	4.459215844664108E-5
10	3.7903334679644917E-4	1.114803961166027E-5	0.0	0.0	4.459215844664108E-5
11	3.846073666022793E-4	1.114803961166027E-5	0.0	0.0	5.0166178252471215E-5
12	3.901813864081094E-4	1.114803961166027E-5	0.0	0.0	6.131421786413148E-5
13	4.0690344582559987E-4	1.6722059417490405E-5	0.0	0.0	7.246225747579176E-5
14	4.0690344582559987E-4	1.6722059417490405E-5	0.0	0.0	7.80362772816219E-5
15	4.0690344582559987E-4	1.6722059417490405E-5	0.0	0.0	7.80362772816219E-5
16	4.180514854372601E-4	3.344411883498081E-5	0.0	0.0	9.475833669911229E-5
17	4.2919952504892037E-4	3.344411883498081E-5	0.0	0.0	1.0033235650494243E-4
18	4.4034756466058067E-4	3.344411883498081E-5	0.0	0.0	1.0033235650494243E-4
19	4.514956042722409E-4	3.344411883498081E-5	0.0	0.0	1.1705441592243283E-4
20	4.7379168349556146E-4	3.344411883498081E-5	0.0	0.0	1.3935049514575336E-4
21	5.239578617480327E-4	3.344411883498081E-5	0.0	0.0	1.3935049514575336E-4
22	5.574019805830135E-4	5.0166178252471215E-5	0.0	0.0	1.560725545632438E-4
23	5.741240400005039E-4	5.0166178252471215E-5	0.0	0.0	1.6722059417490404E-4
24	5.908460994179943E-4	5.0166178252471215E-5	0.0	0.0	1.6722059417490404E-4
25	6.465862974762957E-4	5.0166178252471215E-5	0.0	0.0	1.783686337865643E-4
26	7.524926737870682E-4	5.5740198058301345E-5	0.0	0.0	1.8394265359239446E-4
27	8.249549312628599E-4	5.5740198058301345E-5	0.0	0.0	1.950906932040547E-4
28	8.862691491269914E-4	5.5740198058301345E-5	0.0	0.0	1.950906932040547E-4
29	9.197132679619722E-4	5.5740198058301345E-5	0.0	0.0	2.0066471300988486E-4
30	0.001031193664078575	5.5740198058301345E-5	0.0	0.0	2.0066471300988486E-4
31	0.0011259520007776873	6.131421786413148E-5	0.0	0.0	2.0623873281571498E-4
32	0.0012653024959234406	6.131421786413148E-5	0.0	5.574019805830135E-6	2.396828516506958E-4
33	0.0014269490702925145	6.688823766996162E-5	0.0	1.114803961166027E-5	2.396828516506958E-4
34	0.001566299565438268	6.688823766996162E-5	0.0	1.114803961166027E-5	2.508308912623561E-4
35	0.0017056500605840213	7.246225747579176E-5	0.0	1.114803961166027E-5	2.7312697048567663E-4
36	0.0019397588924288868	7.246225747579176E-5	0.0	1.114803961166027E-5	2.7312697048567663E-4
37	0.0022909221401961853	7.246225747579176E-5	0.0	1.114803961166027E-5	2.842750100973369E-4
38	0.0024971608730119006	7.246225747579176E-5	0.0	1.114803961166027E-5	2.954230497089971E-4
39	0.002759139803885917	7.246225747579176E-5	0.0	1.114803961166027E-5	2.954230497089971E-4
40	0.0029375084376724813	7.80362772816219E-5	0.0	1.114803961166027E-5	3.065710893206574E-4
41	0.0032719496260222893	7.80362772816219E-5	0.0	1.114803961166027E-5	3.28867168543978E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGTAC	61970	0.0	40.405434	19
AAGCAGT	63880	0.0	39.97354	1
GGTATCA	64665	0.0	39.390163	8
TCAACGC	65375	0.0	39.3434	12
TGGTATC	64570	0.0	39.331654	7
GTATCAA	64935	0.0	39.22276	9
ATCAACG	65680	0.0	39.139233	11
ACGCAGA	65500	0.0	39.085342	15
GAGTACT	29585	0.0	38.762886	20
CGCAGAG	66395	0.0	38.530155	16
GTACATG	35350	0.0	37.799435	22
CAGAGTA	66990	0.0	37.654724	18
GAGTACA	36200	0.0	37.418232	20
GCAGAGT	67880	0.0	37.206024	17
AGTACTT	30880	0.0	37.205795	21
GTACTTT	30795	0.0	37.163498	22
ACATGGG	36255	0.0	36.693832	24
TACATGG	36470	0.0	36.535507	23
GTGGTAT	69635	0.0	36.534927	6
AGTGGTA	69835	0.0	36.393284	5
>>END_MODULE