FastQCFastQC Report
Thu 26 May 2016
SRR805360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3900492
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT494511.2678144193091538No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72200.18510485343900207No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC40060.1027049920881776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT175050.043.012851
TCAACGC181500.041.6008312
GGTATCA181500.041.5231448
TGGTATC182150.041.4394727
GTATCAA181850.041.43039
ACGCAGA182300.041.32803315
ATCAACG184300.040.9560511
CGCAGAG184050.040.8584616
AGAGTAC183200.040.2912119
GTGGTAT187950.040.1356746
AGTGGTA188500.040.04355
TATCAAC189450.039.81789410
CAGAGTA186650.039.6094318
CAACGCA191300.039.5802413
GCAGAGT187400.039.56376617
GCAGTGG191700.039.4608763
AACGCAG191950.039.45845414
CAGTGGT193050.039.1727564
GAGTACT91750.038.98310520
AGCAGTG193950.038.9303932
Produced by FastQC (version 0.11.5)