##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805360_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3900492
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.493736174821024	36.0	35.0	37.0	33.0	37.0
2	35.6346027629335	37.0	35.0	37.0	35.0	37.0
3	37.3278999162157	39.0	37.0	39.0	35.0	39.0
4	37.38006256646597	39.0	37.0	39.0	35.0	39.0
5	37.339912759723646	39.0	37.0	39.0	35.0	39.0
6	37.296168534636145	39.0	37.0	39.0	34.0	39.0
7	37.22296289801389	39.0	37.0	39.0	34.0	39.0
8	38.14901991851284	40.0	38.0	40.0	34.0	40.0
9	38.10914007771327	40.0	38.0	40.0	34.0	40.0
10	37.931749635686984	40.0	37.0	40.0	33.0	40.0
11	38.05776399490116	40.0	37.0	40.0	34.0	40.0
12	37.95842883410606	40.0	37.0	40.0	33.0	40.0
13	38.07502412516165	40.0	38.0	40.0	34.0	40.0
14	37.96258318181399	40.0	37.0	40.0	34.0	40.0
15	37.97405891359347	40.0	37.0	40.0	34.0	40.0
16	37.87977978162755	40.0	37.0	40.0	34.0	40.0
17	37.77298145977482	40.0	37.0	40.0	33.0	40.0
18	37.71560639016821	40.0	37.0	40.0	33.0	40.0
19	37.684247269318845	39.0	37.0	40.0	33.0	40.0
20	37.491312121650296	39.0	37.0	40.0	33.0	40.0
21	37.36193587885836	39.0	37.0	40.0	32.0	40.0
22	37.243461594075825	39.0	37.0	40.0	32.0	40.0
23	37.00259685188433	39.0	36.0	40.0	31.0	40.0
24	37.010810687472244	39.0	36.0	40.0	31.0	40.0
25	36.95812964108118	39.0	36.0	40.0	31.0	40.0
26	36.83803222772922	39.0	36.0	40.0	31.0	40.0
27	36.72525619844881	39.0	36.0	40.0	31.0	40.0
28	36.65034180303408	39.0	36.0	40.0	30.0	40.0
29	36.49547646809685	39.0	36.0	40.0	30.0	40.0
30	36.3989289043536	38.0	35.0	40.0	30.0	40.0
31	36.30779732402989	38.0	35.0	40.0	30.0	40.0
32	36.18498153566268	38.0	35.0	40.0	30.0	40.0
33	36.09381201140779	38.0	35.0	40.0	30.0	40.0
34	35.95590940835156	38.0	35.0	40.0	29.0	40.0
35	35.89036998409431	38.0	35.0	40.0	29.0	40.0
36	35.74142954273461	38.0	35.0	40.0	29.0	40.0
37	35.61732084055037	38.0	34.0	40.0	28.0	40.0
38	35.49020303079714	38.0	34.0	40.0	28.0	40.0
39	35.44658750742214	38.0	34.0	40.0	28.0	40.0
40	35.35254783242729	38.0	34.0	40.0	28.0	40.0
41	35.156588194514946	38.0	33.0	40.0	27.0	40.0
42	35.03946322669038	38.0	33.0	40.0	27.0	40.0
43	34.872434554410056	37.0	33.0	40.0	26.0	40.0
44	34.816977960729055	37.0	33.0	40.0	26.0	40.0
45	34.55620778096712	37.0	33.0	40.0	26.0	40.0
46	34.76854740376342	37.0	33.0	40.0	26.0	40.0
47	34.7426258020783	37.0	33.0	40.0	26.0	40.0
48	34.772879164987394	37.0	33.0	40.0	26.0	40.0
49	34.72393251928218	37.0	33.0	40.0	26.0	40.0
50	34.51077428180855	37.0	33.0	40.0	26.0	40.0
51	34.30542352093018	37.0	33.0	40.0	26.0	40.0
52	34.06323125390335	36.0	32.0	39.0	25.0	40.0
53	33.8729116737068	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	7.0
14	22.0
15	40.0
16	96.0
17	246.0
18	535.0
19	1213.0
20	2363.0
21	3937.0
22	6381.0
23	9814.0
24	13959.0
25	19996.0
26	26800.0
27	35002.0
28	47176.0
29	61183.0
30	80154.0
31	105591.0
32	137352.0
33	179701.0
34	236199.0
35	315291.0
36	430095.0
37	600360.0
38	824110.0
39	762866.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.69137944649034	30.51856022265909	25.25919807039727	28.5308622604533
2	22.77351164929963	28.0487179566065	28.83764407156841	20.340126322525464
3	21.317003085764565	25.152185929364805	29.434081649186822	24.096729335683804
4	18.732021498826303	26.484530669464263	29.348118134840423	25.435329696869008
5	22.64619437752981	30.356939586082987	27.23825609692316	19.758609939464048
6	25.241225978671412	25.947213838664457	27.449844788811255	21.36171539385288
7	22.58299722188893	26.59597814839769	30.56752840410902	20.25349622560436
8	24.90155088127344	25.18869414422591	27.4825073349721	22.427247639528552
9	24.863581312306245	24.820945665316067	28.37398461527418	21.941488407103517
10	21.64367982295567	25.856250955007727	30.52986648863784	21.97020273339876
11	21.397095545895237	29.9099959697392	26.645330896717645	22.04757758764792
12	21.451345112360183	25.90462946725695	30.369989221872522	22.27403619851034
13	21.716644977095196	26.22740926016513	25.940086532673313	26.115859230066363
14	21.92087562287014	29.987499012945033	25.87306934612352	22.21855601806131
15	21.840808800530805	30.001856176092655	25.824511369334946	22.332823654041594
16	22.034425400693042	26.06965993008061	25.67258181788349	26.223332851342857
17	26.175185079215645	25.765775189386364	25.703167702946196	22.355872028451795
18	22.046244422498496	25.81807628370985	25.651353726658073	26.48432556713358
19	22.369511333442038	29.69453597135951	25.487323137696478	22.448629557501974
20	26.49229892023878	25.73613790260306	25.49578360883704	22.275779568321123
21	22.527824694935923	29.675384541232237	25.57215858922413	22.224632174607716
22	26.48032607168532	25.616409417068407	25.668761786974564	22.234502724271707
23	22.466473460271168	25.583387941828878	29.818289590133755	22.1318490077662
24	22.23806637726728	29.633800043686797	25.607872032553843	22.520261546492083
25	22.33531051980109	25.670633345742022	25.537060452886458	26.456995681570426
26	22.32497848989307	27.587442814906428	27.685866295841656	22.40171239935885
27	22.420248522494084	25.53734246859114	29.729634107697184	22.31277490121759
28	24.59574330622906	25.507653906225165	27.53375727985085	22.362845507694924
29	24.760466115556703	25.514832487798977	27.48789127115246	22.23681012549186
30	24.581539969829446	25.42897152461792	27.495018577143604	22.494469928409032
31	23.78671716286048	25.90950064761061	27.51363161365284	22.79015057587607
32	23.45055444287541	26.35057320973867	27.673560156000832	22.525312191385087
33	23.19538150571774	26.207539971880472	27.7115810005507	22.88549752185109
34	23.124108445806325	26.05299536571284	27.84628195622501	22.97661423225583
35	23.04150348212482	26.312372900649457	27.82692542376705	22.81919819345867
36	22.950514960676756	26.257738767314482	27.857895875699786	22.933850396308976
37	23.03881151403464	26.17285204020416	27.817644543303764	22.970691902457432
38	23.216455770195143	26.223332851342857	27.78423850119421	22.77597287726779
39	23.366667589627156	26.148521776227202	27.621105234929338	22.863705399216304
40	23.125185233042394	26.159238373005252	27.665740629643643	23.049835764308707
41	23.127159342975194	26.406514870431728	27.67868771426784	22.787638072325233
42	23.15002825284605	26.258148971975842	27.731757942331374	22.860064832846728
43	23.175819870929104	26.199335878653258	27.752139986442735	22.872704263974903
44	23.254040772292317	26.14554779243234	27.621771817504047	22.978639617771297
45	23.23045400426408	26.123755669797553	27.643461388973495	23.002328936964876
46	22.978793444519308	26.283120180736177	27.6215154395907	23.11657093515382
47	23.073935288163646	26.30175885503675	27.61323443298948	23.011071423810126
48	23.173794485413634	26.21033449113599	27.641974397076062	22.973896626374312
49	23.101188260352796	26.48594074798769	27.40174829226672	23.011122699392793
50	23.195560970257084	26.390157959559975	27.499735930749246	22.91454513943369
51	23.132927846025577	26.29278562806949	27.480584500621973	23.093702025282965
52	23.305444543919073	26.44158736897807	27.096684213171056	23.1562838739318
53	23.34913134035399	26.470352970855988	27.019437547878578	23.161078140911453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7890.0
1	4610.5
2	1331.0
3	1080.0
4	829.0
5	825.5
6	822.0
7	969.5
8	1351.0
9	1585.0
10	1931.5
11	2278.0
12	3063.5
13	3849.0
14	4804.0
15	5759.0
16	7928.5
17	10098.0
18	12964.0
19	15830.0
20	20799.0
21	25768.0
22	59232.5
23	92697.0
24	74622.5
25	63968.5
26	71389.0
27	79021.0
28	86653.0
29	90891.5
30	95130.0
31	99008.5
32	102887.0
33	110771.5
34	118656.0
35	129022.0
36	139388.0
37	151212.0
38	163036.0
39	174262.5
40	185489.0
41	192940.5
42	207670.0
43	214948.0
44	223507.5
45	232067.0
46	235921.5
47	239776.0
48	253425.0
49	267074.0
50	267509.5
51	267945.0
52	260713.5
53	253482.0
54	240194.5
55	226907.0
56	211917.0
57	196927.0
58	182283.0
59	153929.5
60	140220.0
61	123272.0
62	106324.0
63	92478.0
64	78632.0
65	64266.0
66	49900.0
67	40944.5
68	31989.0
69	25135.0
70	18281.0
71	14279.0
72	10277.0
73	7126.0
74	3975.0
75	2938.5
76	1506.0
77	1110.0
78	867.0
79	624.0
80	579.5
81	535.0
82	431.5
83	328.0
84	233.5
85	139.0
86	84.0
87	29.0
88	27.0
89	25.0
90	17.5
91	10.0
92	7.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	3900492.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.329042558506316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.85202794302123	44.41659237826972
2	12.994768143509884	14.639656955873805
3	3.594395022912629	6.074064906531863
4	1.526155281998381	3.43867463322304
5	0.809903054397265	2.28105318097039
6	0.4620088496511379	1.5614709692643296
7	0.30690742380045605	1.2101460935744197
8	0.21927526338953993	0.9881252514797659
9	0.16519830105379735	0.837491591758706
>10	0.8453789336477869	9.156845714393326
>50	0.13695427725592463	5.455630001071058
>100	0.08614365229603037	7.880917471226098
>500	3.721486647046439E-4	0.14010996653440808
>1k	4.186672373333968E-4	0.43424393484646323
>5k	4.651858192593298E-5	0.1891890664730548
>10k+	4.651858192593298E-5	1.2957878845095616
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49451	1.2678144193091538	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7220	0.18510485343900207	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	4006	0.1027049920881776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0255116534016734E-4	0.0	0.0	0.0	0.0
2	1.2818895667520918E-4	2.5637791335041835E-5	0.0	0.0	0.0
3	1.2818895667520918E-4	2.5637791335041835E-5	0.0	0.0	2.5637791335041835E-5
4	1.2818895667520918E-4	2.5637791335041835E-5	0.0	0.0	5.127558267008367E-5
5	4.61480244030753E-4	2.5637791335041835E-5	0.0	0.0	5.127558267008367E-5
6	4.61480244030753E-4	2.5637791335041835E-5	0.0	0.0	7.691337400512551E-5
7	4.8711803536579485E-4	2.5637791335041835E-5	0.0	0.0	7.691337400512551E-5
8	4.8711803536579485E-4	2.5637791335041835E-5	0.0	0.0	7.691337400512551E-5
9	4.8711803536579485E-4	2.5637791335041835E-5	0.0	0.0	7.691337400512551E-5
10	5.127558267008367E-4	2.5637791335041835E-5	0.0	0.0	1.0255116534016734E-4
11	5.383936180358785E-4	2.5637791335041835E-5	0.0	2.5637791335041835E-5	1.0255116534016734E-4
12	6.409447833760458E-4	5.127558267008367E-5	0.0	2.5637791335041835E-5	1.2818895667520918E-4
13	7.434959487162133E-4	5.127558267008367E-5	0.0	2.5637791335041835E-5	1.5382674801025102E-4
14	7.947715313862969E-4	7.691337400512551E-5	0.0	2.5637791335041835E-5	1.5382674801025102E-4
15	9.22960488061506E-4	1.0255116534016734E-4	0.0	2.5637791335041835E-5	1.7946453934529285E-4
16	9.998738620666317E-4	1.0255116534016734E-4	0.0	2.5637791335041835E-5	2.0510233068033468E-4
17	0.001102425027406799	1.0255116534016734E-4	0.0	2.5637791335041835E-5	2.0510233068033468E-4
18	0.0011793384014119244	1.0255116534016734E-4	0.0	2.5637791335041835E-5	2.0510233068033468E-4
19	0.00125625177541705	1.0255116534016734E-4	0.0	2.5637791335041835E-5	2.0510233068033468E-4
20	0.0012818895667520917	1.0255116534016734E-4	0.0	2.5637791335041835E-5	2.0510233068033468E-4
21	0.0013844407320922592	1.0255116534016734E-4	0.0	2.5637791335041835E-5	2.0510233068033468E-4
22	0.0015126296887674683	1.0255116534016734E-4	0.0	2.5637791335041835E-5	2.0510233068033468E-4
23	0.0015126296887674683	1.0255116534016734E-4	0.0	2.5637791335041835E-5	2.0510233068033468E-4
24	0.0017946453934529285	1.5382674801025102E-4	0.0	2.5637791335041835E-5	2.0510233068033468E-4
25	0.001871558767458054	1.5382674801025102E-4	0.0	2.5637791335041835E-5	2.0510233068033468E-4
26	0.002153574472143514	1.5382674801025102E-4	0.0	2.5637791335041835E-5	2.5637791335041837E-4
27	0.0022561256374836816	1.5382674801025102E-4	0.0	2.5637791335041835E-5	2.820157046854602E-4
28	0.002333039011488807	1.5382674801025102E-4	0.0	2.5637791335041835E-5	2.820157046854602E-4
29	0.0023843145941588907	1.7946453934529285E-4	0.0	2.5637791335041835E-5	2.820157046854602E-4
30	0.0024868657594990583	1.7946453934529285E-4	0.0	2.5637791335041835E-5	3.0765349602050203E-4
31	0.002948346003529811	2.0510233068033468E-4	0.0	2.5637791335041835E-5	3.3329128735554386E-4
32	0.0029996215861998947	2.0510233068033468E-4	0.0	2.5637791335041835E-5	3.3329128735554386E-4
33	0.003307275082220397	2.0510233068033468E-4	0.0	2.5637791335041835E-5	3.3329128735554386E-4
34	0.004460975692297279	2.307401220153765E-4	0.0	2.5637791335041835E-5	3.3329128735554386E-4
35	0.005178833849678451	2.307401220153765E-4	0.0	2.5637791335041835E-5	3.589290786905857E-4
36	0.005486487345698953	2.307401220153765E-4	0.0	2.5637791335041835E-5	3.8456687002562753E-4
37	0.006229983294415166	2.307401220153765E-4	0.0	2.5637791335041835E-5	3.8456687002562753E-4
38	0.007358046113157007	2.307401220153765E-4	0.0	2.5637791335041835E-5	4.1020466136066936E-4
39	0.008306644392553554	2.307401220153765E-4	0.0	2.5637791335041835E-5	4.358424526957112E-4
40	0.009332156045955229	2.307401220153765E-4	0.0	2.5637791335041835E-5	4.358424526957112E-4
41	0.010972974691397906	2.5637791335041837E-4	0.0	2.5637791335041835E-5	4.358424526957112E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	17505	0.0	43.01285	1
TCAACGC	18150	0.0	41.60083	12
GGTATCA	18150	0.0	41.523144	8
TGGTATC	18215	0.0	41.439472	7
GTATCAA	18185	0.0	41.4303	9
ACGCAGA	18230	0.0	41.328033	15
ATCAACG	18430	0.0	40.95605	11
CGCAGAG	18405	0.0	40.85846	16
AGAGTAC	18320	0.0	40.29121	19
GTGGTAT	18795	0.0	40.135674	6
AGTGGTA	18850	0.0	40.0435	5
TATCAAC	18945	0.0	39.817894	10
CAGAGTA	18665	0.0	39.60943	18
CAACGCA	19130	0.0	39.58024	13
GCAGAGT	18740	0.0	39.563766	17
GCAGTGG	19170	0.0	39.460876	3
AACGCAG	19195	0.0	39.458454	14
CAGTGGT	19305	0.0	39.172756	4
GAGTACT	9175	0.0	38.983105	20
AGCAGTG	19395	0.0	38.930393	2
>>END_MODULE