FastQCFastQC Report
Thu 26 May 2016
SRR805359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18407428
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1538100.835586590369931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT908100.040.4838681
TCAACGC933400.039.9983412
GGTATCA936800.039.649988
ACGCAGA941300.039.63768415
GTATCAA937550.039.605739
CAACGCA943500.039.6000513
ATCAACG945850.039.4668811
GTGGTAT942450.039.3474436
AACGCAG949750.039.3122414
TGGTATC943800.039.2861827
AGTGGTA942350.039.2369085
AGAGTAC929650.039.09041219
CGCAGAG954150.039.04475416
TATCAAC961650.038.6156110
CAGAGTA952800.038.25162518
AGCAGTG968400.038.2396742
CAGTGGT969650.038.2291534
GCAGTGG980950.038.0139663
GCAGAGT966800.037.81925217
GTACATG657050.037.6579422
Produced by FastQC (version 0.11.5)