##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805359_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18407428
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.49999038431659	36.0	35.0	37.0	33.0	37.0
2	35.490617103052095	37.0	35.0	37.0	33.0	37.0
3	37.20336377249445	39.0	37.0	39.0	33.0	39.0
4	37.22619504473955	39.0	37.0	39.0	34.0	39.0
5	37.23184955551639	39.0	37.0	39.0	34.0	39.0
6	37.16348997806755	39.0	37.0	39.0	33.0	39.0
7	37.04148173226591	39.0	37.0	39.0	33.0	39.0
8	37.925998569707836	40.0	38.0	40.0	33.0	40.0
9	37.859023270388455	40.0	38.0	40.0	33.0	40.0
10	37.663925780396916	40.0	37.0	40.0	33.0	40.0
11	37.73018462981357	40.0	37.0	40.0	33.0	40.0
12	37.660868482006286	40.0	37.0	40.0	33.0	40.0
13	37.758040232453986	40.0	37.0	40.0	33.0	40.0
14	37.653492220640494	40.0	37.0	40.0	33.0	40.0
15	37.62057686712125	40.0	37.0	40.0	33.0	40.0
16	37.50042482849858	40.0	37.0	40.0	32.0	40.0
17	37.3776496640378	39.0	37.0	40.0	32.0	40.0
18	37.338320160752495	39.0	37.0	40.0	32.0	40.0
19	37.303800074622046	39.0	37.0	40.0	32.0	40.0
20	37.10958842267372	39.0	37.0	40.0	32.0	40.0
21	37.0224260010687	39.0	36.0	40.0	31.0	40.0
22	36.89081494709636	39.0	36.0	40.0	31.0	40.0
23	36.682294886607735	39.0	36.0	40.0	30.0	40.0
24	36.71316155630216	39.0	36.0	40.0	30.0	40.0
25	36.68081792850147	39.0	36.0	40.0	30.0	40.0
26	36.56685947651133	39.0	36.0	40.0	30.0	40.0
27	36.43321076687085	39.0	36.0	40.0	30.0	40.0
28	36.32661820000057	39.0	35.0	40.0	30.0	40.0
29	36.2216721966806	38.0	35.0	40.0	30.0	40.0
30	36.105827332313886	38.0	35.0	40.0	30.0	40.0
31	35.96688288010688	38.0	35.0	40.0	29.0	40.0
32	35.87927324773455	38.0	35.0	40.0	29.0	40.0
33	35.76373135888403	38.0	35.0	40.0	29.0	40.0
34	35.79832760991921	38.0	35.0	40.0	29.0	40.0
35	35.82743450089822	38.0	35.0	40.0	29.0	40.0
36	35.80575205835383	38.0	35.0	40.0	29.0	40.0
37	35.77193364548268	38.0	35.0	40.0	29.0	40.0
38	35.71925741064966	38.0	35.0	40.0	28.0	40.0
39	35.65534549422114	38.0	34.0	40.0	28.0	40.0
40	35.606971109706365	38.0	34.0	40.0	28.0	40.0
41	35.55507358225169	38.0	34.0	40.0	28.0	40.0
42	35.321982788687265	38.0	34.0	40.0	27.0	40.0
43	35.333752439504316	38.0	34.0	40.0	27.0	40.0
44	35.22187999322882	38.0	34.0	40.0	27.0	40.0
45	35.08368132690781	38.0	33.0	40.0	26.0	40.0
46	34.93692051925994	38.0	33.0	40.0	26.0	40.0
47	35.12216095589237	38.0	34.0	40.0	26.0	40.0
48	35.177383445422144	38.0	34.0	40.0	27.0	40.0
49	35.07272759670715	38.0	34.0	40.0	26.0	40.0
50	34.972389407145855	38.0	33.0	40.0	26.0	40.0
51	34.8108179480588	38.0	33.0	40.0	26.0	40.0
52	34.5968559540203	37.0	33.0	40.0	25.0	40.0
53	34.11010299755077	37.0	32.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	7.0
11	16.0
12	26.0
13	88.0
14	225.0
15	573.0
16	1363.0
17	3264.0
18	6961.0
19	13251.0
20	22449.0
21	35626.0
22	52085.0
23	73017.0
24	98874.0
25	128299.0
26	164495.0
27	207741.0
28	259601.0
29	321054.0
30	396270.0
31	487867.0
32	603587.0
33	757462.0
34	975205.0
35	1299615.0
36	1803444.0
37	2646660.0
38	3963045.0
39	4085256.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	16.243828306703143	26.397772681767385	33.434866620149215	23.923532391380263
2	25.325786959481793	27.682797401136106	25.309592410194405	21.681823229187696
3	23.374667009426847	24.608978505851006	28.27298849138511	23.743365993337036
4	20.902578024480118	25.520012899140497	27.740790294005222	25.83661878237416
5	24.153064730173057	29.47695354288497	26.13149974021357	20.238481986728402
6	26.585436053314996	25.74088569027677	25.96653372757998	21.707144528828255
7	23.358972258373086	25.61243211164536	30.42524463493759	20.603350995043957
8	26.22521190901847	24.931842732183988	26.13342830948463	22.709517049312918
9	26.05315093450318	24.568690422149146	26.992271815486664	22.38588682786101
10	22.65816821339733	25.098487414971828	29.86160260955523	22.381741762075613
11	22.11161168197969	29.330588716685458	26.115571387811485	22.442228213523364
12	22.08389461037142	25.50666502674898	30.048212058740635	22.36122830413896
13	22.207029683886308	25.272726857874982	25.858691393496148	26.661552064742562
14	22.61417510365924	29.376369148367715	25.42267176055232	22.58678398742073
15	22.288360981229967	29.635639482061265	25.601425685326596	22.47457385138217
16	22.40740531485442	25.57939110233108	25.402500555753903	26.610703027060595
17	26.850845213138957	25.00838248559223	25.41321905482939	22.727553246439427
18	22.832907454534116	25.02708145863724	25.387876024830845	26.7521350619978
19	22.575413577605737	29.163998359792583	25.328188164038995	22.93239989856269
20	26.919453385883134	25.209545842037244	25.2168309445513	22.65416982752832
21	23.066286066690033	29.079624812331197	25.30433366356234	22.549755457416428
22	26.86914760715077	25.1264598182864	25.433993277061845	22.570399297500988
23	23.092628693155827	24.944647345625906	29.354307402424716	22.608416558793547
24	22.642147506973814	29.165769383968254	25.501438875653893	22.690644233404036
25	22.604342116671596	25.319419964592555	25.271466497111927	26.804771421623926
26	22.879193117039488	27.705913069441312	26.473340001655853	22.94155381186334
27	22.83146781831769	25.069901129044208	29.502470415747382	22.596160636890716
28	25.860125597123073	24.84744745436462	26.696049008041754	22.596377940470553
29	26.09128771276465	24.884367332579	26.355871118985224	22.668473835671122
30	25.860869861884016	24.882406167770966	26.53063752306949	22.726086447275524
31	24.825412871369103	25.290170902746432	26.794753726593417	23.089662499291048
32	24.381554011782637	25.857990589451173	26.697711380427506	23.062744018338684
33	23.945947255640494	25.82321658408768	26.92259885520128	23.308237305070538
34	23.648980183434645	25.644685395482735	27.131503651678006	23.574830769404613
35	23.63342124711828	25.811960258652107	27.1476873357864	23.40693115844321
36	23.512198445105962	25.884376676632932	27.18716596365337	23.41625891460773
37	23.78771765398186	25.722273638663694	27.08375119000873	23.406257517345715
38	23.95782289627861	25.80493592043386	26.978396981914038	23.25884420137349
39	23.904849716103737	25.791924868591092	26.967624156943597	23.33560125836157
40	23.80195647105071	25.80854315985916	26.877155244067772	23.512345125022353
41	23.835790638431398	26.03396846099303	26.845624494633363	23.28461640594221
42	23.929399588035874	25.91601607785726	26.981694563738074	23.172889770368787
43	23.764802991488	25.78987134976163	27.092801884108958	23.352523774641412
44	23.89149097853323	25.658136487074675	26.961213701338394	23.4891588330537
45	23.826071735823167	25.58390015161271	27.06820311887136	23.521824993692764
46	23.812256660735002	25.642995860149497	26.90416607904157	23.640581400073927
47	23.808752640510125	25.646907324586575	26.940167849631138	23.604172185272162
48	23.68903466578818	25.651378345741733	27.042474375018603	23.617112613451482
49	23.644769926575293	25.941478624824715	26.798100201722914	23.615651246877075
50	23.929106228203093	25.889075866546918	26.804608443939042	23.377209461310944
51	23.76083720115597	25.788431713545208	26.90116728963981	23.54956379565901
52	23.932235399752752	25.760214843703316	26.68656370678185	23.620986049762084
53	24.14680638707374	25.871838260076313	26.408768242907154	23.57258710994279
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20129.0
1	11828.0
2	3527.0
3	2867.5
4	2208.0
5	2198.5
6	2189.0
7	2519.0
8	3342.0
9	3835.0
10	4976.0
11	6117.0
12	8342.5
13	10568.0
14	13446.5
15	16325.0
16	23209.5
17	30094.0
18	40606.0
19	51118.0
20	67582.0
21	84046.0
22	197208.5
23	310371.0
24	259639.0
25	234885.5
26	260864.0
27	292537.5
28	324211.0
29	351852.5
30	379494.0
31	404526.0
32	429558.0
33	466260.5
34	502963.0
35	557499.5
36	612036.0
37	668800.5
38	725565.0
39	780665.0
40	835765.0
41	891130.0
42	998053.5
43	1049612.0
44	1102917.0
45	1156222.0
46	1193470.5
47	1230719.0
48	1268624.0
49	1306529.0
50	1316688.0
51	1326847.0
52	1304667.0
53	1282487.0
54	1234818.0
55	1187149.0
56	1121479.5
57	1055810.0
58	958891.0
59	769802.5
60	677633.0
61	601398.0
62	525163.0
63	453809.5
64	382456.0
65	313111.0
66	243766.0
67	201098.5
68	158431.0
69	126008.5
70	93586.0
71	71880.5
72	50175.0
73	36538.0
74	22901.0
75	17188.0
76	8940.5
77	6406.0
78	5000.0
79	3594.0
80	3136.5
81	2679.0
82	2152.0
83	1625.0
84	1089.0
85	553.0
86	383.5
87	214.0
88	159.0
89	104.0
90	81.5
91	59.0
92	40.5
93	12.0
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8407428E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.92747420640151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.90843301108346	40.45588145644197
2	12.161091354848555	12.629895153011816
3	3.877688618841879	6.040757272061051
4	1.736375292746858	3.606623329069815
5	0.9673714170903063	2.5116577154483477
6	0.6022966758107988	1.876544705865998
7	0.42133412475032483	1.5315171826674479
8	0.30359719449022354	1.2612028388821557
9	0.24299752734864652	1.1356423050254572
>10	1.5904329876089165	15.523010702706747
>50	0.11538305323788645	4.141203086698413
>100	0.0689079581246793	6.215511480543652
>500	0.0032511576044295847	1.1650693258348725
>1k	7.452516908361137E-4	0.5531134562747215
>5k	3.1458240790379913E-5	0.11046589243252224
>10k+	6.291648158075793E-5	1.2419040970348958
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	153810	0.835586590369931	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.857138542114629E-4	5.432589495936097E-6	0.0	5.432589495936097E-6	1.629776848780829E-5
2	4.509049281626961E-4	5.432589495936097E-6	0.0	5.432589495936097E-6	1.629776848780829E-5
3	4.617701071545683E-4	5.432589495936097E-6	0.0	1.0865178991872194E-5	2.7162947479680486E-5
4	4.780678756423765E-4	5.432589495936097E-6	0.0	1.629776848780829E-5	3.259553697561658E-5
5	0.001059354951707539	5.432589495936097E-6	0.0	1.629776848780829E-5	5.432589495936097E-5
6	0.001064787541203475	5.432589495936097E-6	0.0	1.629776848780829E-5	6.519107395123316E-5
7	0.001119113436162836	5.432589495936097E-6	0.0	2.1730357983744387E-5	7.605625294310536E-5
8	0.0011354112046506443	1.0865178991872194E-5	0.0	2.1730357983744387E-5	7.605625294310536E-5
9	0.0011625741521303248	1.0865178991872194E-5	0.0	2.1730357983744387E-5	7.605625294310536E-5
10	0.001222332636585622	1.0865178991872194E-5	0.0	2.1730357983744387E-5	7.605625294310536E-5
11	0.001330984426504344	1.0865178991872194E-5	0.0	2.1730357983744387E-5	8.692143193497755E-5
12	0.001510259879870235	1.0865178991872194E-5	0.0	2.1730357983744387E-5	9.778661092684975E-5
13	0.0018470804286182731	1.0865178991872194E-5	0.0	2.1730357983744387E-5	9.778661092684975E-5
14	0.0020861143664394613	1.0865178991872194E-5	0.0	2.1730357983744387E-5	1.0865178991872195E-4
15	0.0023251483042606496	1.0865178991872194E-5	0.0	2.1730357983744387E-5	1.1408437941465804E-4
16	0.0025261541156102853	1.0865178991872194E-5	0.0	2.1730357983744387E-5	1.1951696891059413E-4
17	0.0027760532324233454	1.0865178991872194E-5	0.0	2.1730357983744387E-5	1.2494955840653023E-4
18	0.0030476827072201504	1.0865178991872194E-5	0.0	2.1730357983744387E-5	1.3038214790246633E-4
19	0.0032269581605860417	1.0865178991872194E-5	0.0	2.7162947479680486E-5	1.4667991639027463E-4
20	0.0035420483513503354	1.0865178991872194E-5	0.0	3.802812647155268E-5	1.5211250588621073E-4
21	0.0037810822891715234	1.0865178991872194E-5	0.0	3.802812647155268E-5	1.5211250588621073E-4
22	0.004030981405984584	1.0865178991872194E-5	0.0	3.802812647155268E-5	1.5754509538214683E-4
23	0.004329773828261069	1.0865178991872194E-5	0.0	3.802812647155268E-5	1.738428638699551E-4
24	0.004623133661041619	1.0865178991872194E-5	0.0	4.889330546342487E-5	1.738428638699551E-4
25	0.005084903768196187	1.0865178991872194E-5	0.0	4.889330546342487E-5	1.738428638699551E-4
26	0.0055303761068629465	1.0865178991872194E-5	0.0	4.889330546342487E-5	1.738428638699551E-4
27	0.0059704158560337705	1.0865178991872194E-5	0.0	5.432589495936097E-5	1.738428638699551E-4
28	0.006285506046798064	1.629776848780829E-5	0.0	5.432589495936097E-5	1.847080428618273E-4
29	0.006763573922440441	1.629776848780829E-5	0.0	5.432589495936097E-5	1.901406323577634E-4
30	0.007181883313627521	1.629776848780829E-5	0.0	5.9758484455297065E-5	2.064384008455717E-4
31	0.0076056252943105356	1.629776848780829E-5	0.0	5.9758484455297065E-5	2.281687588293161E-4
32	0.008040232453985423	2.1730357983744387E-5	0.0	5.9758484455297065E-5	2.336013483252522E-4
33	0.00854546327710748	2.1730357983744387E-5	0.0	6.519107395123316E-5	2.4446652731712436E-4
34	0.008980070436782369	2.1730357983744387E-5	0.0	6.519107395123316E-5	2.5533170630899656E-4
35	0.009490733849400362	2.1730357983744387E-5	0.0	6.519107395123316E-5	2.6076429580493266E-4
36	0.009963369135546803	2.1730357983744387E-5	0.0	6.519107395123316E-5	2.6619688530086876E-4
37	0.010425139242701371	2.1730357983744387E-5	0.0	6.519107395123316E-5	2.7162947479680486E-4
38	0.010930370065823427	2.7162947479680486E-5	0.0	7.062366344716926E-5	2.7706206429274096E-4
39	0.01144103347844142	3.259553697561658E-5	0.0	7.605625294310536E-5	2.8792724328461315E-4
40	0.011967994659547222	3.259553697561658E-5	0.0	7.605625294310536E-5	2.8792724328461315E-4
41	0.012522118788132704	3.802812647155268E-5	0.0	7.605625294310536E-5	2.8792724328461315E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	90810	0.0	40.483868	1
TCAACGC	93340	0.0	39.99834	12
GGTATCA	93680	0.0	39.64998	8
ACGCAGA	94130	0.0	39.637684	15
GTATCAA	93755	0.0	39.60573	9
CAACGCA	94350	0.0	39.60005	13
ATCAACG	94585	0.0	39.46688	11
GTGGTAT	94245	0.0	39.347443	6
AACGCAG	94975	0.0	39.31224	14
TGGTATC	94380	0.0	39.286182	7
AGTGGTA	94235	0.0	39.236908	5
AGAGTAC	92965	0.0	39.090412	19
CGCAGAG	95415	0.0	39.044754	16
TATCAAC	96165	0.0	38.61561	10
CAGAGTA	95280	0.0	38.251625	18
AGCAGTG	96840	0.0	38.239674	2
CAGTGGT	96965	0.0	38.229153	4
GCAGTGG	98095	0.0	38.013966	3
GCAGAGT	96680	0.0	37.819252	17
GTACATG	65705	0.0	37.65794	22
>>END_MODULE