FastQCFastQC Report
Thu 26 May 2016
SRR805358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25231873
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6800622.6952497739664434No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG1102810.4370702087791897No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC966010.38285306841866235No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG612380.2427009679384483No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG379090.15024251271397887No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA283730.1124490441117867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3981250.045.6146431
TCAACGC4034900.045.4484412
GTATCAA4007050.045.448219
TGGTATC4005650.045.4042557
GGTATCA4013250.045.3943948
GTGGTAT4011000.045.3507236
ACGCAGA4039300.045.348915
ATCAACG4043150.045.2882811
CAACGCA4050500.045.28790713
AGTGGTA4009800.045.2752155
AACGCAG4056950.045.18346414
CGCAGAG4053250.045.1383216
AGAGTAC3936150.045.12532819
TATCAAC4037500.045.0996310
AGCAGTG4056400.044.835622
CAGAGTA3979850.044.81583418
CAGTGGT4068750.044.7076154
GCAGAGT4003950.044.70631817
GCAGTGG4102000.044.6305243
GTACATG2825850.044.58251622