##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805358_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25231873
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.69534235528214	37.0	35.0	37.0	35.0	37.0
2	35.60508405380766	37.0	35.0	37.0	33.0	37.0
3	37.30633358054711	39.0	37.0	39.0	34.0	39.0
4	37.247983770368535	39.0	37.0	39.0	34.0	39.0
5	37.223395861258496	39.0	37.0	39.0	34.0	39.0
6	37.227331478721375	39.0	37.0	39.0	34.0	39.0
7	36.92619069539546	39.0	37.0	39.0	33.0	39.0
8	37.91496239696514	40.0	38.0	40.0	33.0	40.0
9	37.89332773670825	40.0	38.0	40.0	33.0	40.0
10	37.376855455795926	39.0	37.0	40.0	32.0	40.0
11	37.63006662248181	40.0	37.0	40.0	33.0	40.0
12	37.70096988043654	40.0	37.0	40.0	33.0	40.0
13	37.79500820252226	40.0	37.0	40.0	33.0	40.0
14	37.7296600216718	40.0	37.0	40.0	33.0	40.0
15	37.69896602602589	40.0	37.0	40.0	33.0	40.0
16	37.5314651829454	40.0	37.0	40.0	32.0	40.0
17	37.40900570480836	40.0	37.0	40.0	32.0	40.0
18	37.363756824552816	40.0	37.0	40.0	32.0	40.0
19	37.328231756715006	39.0	37.0	40.0	32.0	40.0
20	37.18539016108713	39.0	37.0	40.0	32.0	40.0
21	36.89589884983965	39.0	36.0	40.0	31.0	40.0
22	36.951902936416964	39.0	36.0	40.0	31.0	40.0
23	36.27978117201208	39.0	36.0	40.0	30.0	40.0
24	36.551953753096335	39.0	36.0	40.0	30.0	40.0
25	36.65231582292761	39.0	36.0	40.0	30.0	40.0
26	36.58882255788146	39.0	36.0	40.0	30.0	40.0
27	36.52141198554701	39.0	36.0	40.0	30.0	40.0
28	36.46314746432023	39.0	36.0	40.0	30.0	40.0
29	36.35272752046588	39.0	35.0	40.0	30.0	40.0
30	36.24335553686403	39.0	35.0	40.0	30.0	40.0
31	36.00093120316514	38.0	35.0	40.0	29.0	40.0
32	35.88485139410776	38.0	35.0	40.0	29.0	40.0
33	35.68536374608417	38.0	35.0	40.0	28.0	40.0
34	35.682693115964874	38.0	35.0	40.0	28.0	40.0
35	35.69415477004026	38.0	35.0	40.0	28.0	40.0
36	35.65648007977846	38.0	35.0	40.0	28.0	40.0
37	35.5688738604542	38.0	34.0	40.0	28.0	40.0
38	35.441399177936574	38.0	34.0	40.0	27.0	40.0
39	35.36412786319906	38.0	34.0	40.0	27.0	40.0
40	35.31522495377176	38.0	34.0	40.0	27.0	40.0
41	35.25315786109101	38.0	34.0	40.0	27.0	40.0
42	35.03192481984988	38.0	33.0	40.0	26.0	40.0
43	35.01640940408982	38.0	33.0	40.0	26.0	40.0
44	34.917816049565566	38.0	33.0	40.0	26.0	40.0
45	34.78981104573568	37.0	33.0	40.0	26.0	40.0
46	34.62739139500266	37.0	33.0	40.0	26.0	40.0
47	34.773906954905804	37.0	33.0	40.0	26.0	40.0
48	34.81179887042076	37.0	33.0	40.0	26.0	40.0
49	34.69284519623256	37.0	33.0	40.0	26.0	40.0
50	34.549365122438594	37.0	33.0	40.0	25.0	40.0
51	34.30584550738663	37.0	33.0	40.0	24.0	40.0
52	34.019336654080334	37.0	33.0	40.0	23.0	40.0
53	33.48615717113034	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	17.0
12	36.0
13	92.0
14	292.0
15	783.0
16	2001.0
17	5015.0
18	10624.0
19	20476.0
20	34982.0
21	54818.0
22	79655.0
23	110364.0
24	148427.0
25	193844.0
26	248982.0
27	309120.0
28	380871.0
29	462351.0
30	561358.0
31	680286.0
32	835525.0
33	1046938.0
34	1350087.0
35	1816941.0
36	2563299.0
37	3749911.0
38	5213672.0
39	5351105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.878384177028792	37.870965821681175	27.367999989537044	19.882650011752993
2	23.991306551043596	40.23964847952429	17.43098104528348	18.338063924148635
3	37.96859234350141	22.35587504740532	19.184655851747507	20.49087675734576
4	21.035501407287523	21.604000622545936	19.921473923081333	37.439024047085205
5	21.12159093381613	37.40086596028761	21.19664283345117	20.280900272445095
6	36.86195234099347	21.84069727998393	20.319831191287303	20.97751918773529
7	21.405133102881425	21.429463440942335	36.96407317839623	20.201330277780013
8	36.069997657328095	22.38487012042269	21.337758001556207	20.207374220693012
9	35.978129725050536	22.03329098874269	21.62490275692177	20.363676529285005
10	20.469170084995277	22.284612006409514	37.03109951449105	20.215118394104156
11	20.25492915250485	37.55449704427412	21.88902900708164	20.30154479613939
12	20.147580799887507	22.179625745579806	37.294171542477244	20.37862191205544
13	20.2052182174506	22.143155999556594	22.007854906371794	35.64377087662101
14	20.364528626154705	37.56629561348854	21.75017288649162	20.31900287386513
15	20.20101321847966	37.5379505120369	21.749550657614677	20.511485611868764
16	20.34452218430237	22.01486588015087	21.875708553225518	35.764903382321236
17	35.907183743355084	21.81687820004484	21.78182729439071	20.49411076220937
18	20.503277739230853	21.855761559991997	21.798330230974134	35.84263046980302
19	20.482494502092653	37.35834434486889	21.769854342561093	20.38930681047737
20	36.047736131201994	22.00760918541402	21.74705381562439	20.1976008677596
21	20.679186202308486	37.222817346932594	21.71802307343573	20.3799733773232
22	35.90228121392336	21.840970743630486	21.97159917537632	20.285148867069836
23	20.910373954402832	21.860382699294657	36.90101404679708	20.32822929950543
24	20.582946814927293	37.086251979787626	21.78763344282844	20.543167762456633
25	20.41355788371319	22.093892910764097	21.76954520974325	35.723003995779465
26	20.644262120374496	32.83912771754994	26.030469478028838	20.48614068404672
27	20.839102986924516	21.904854229410557	36.88390473430173	20.3721380493632
28	31.807939902043735	21.756815278834036	26.060086779923154	20.37515803919907
29	31.97462986596358	21.840709169707694	25.803348011461537	20.38131295286719
30	31.787580731719757	21.84683237744578	25.79871498243511	20.56687190839935
31	27.802212701371793	23.308495568283814	26.902977040190397	21.986314690153996
32	25.529555415882125	25.03728518291131	27.36193226717652	22.071227134030043
33	25.041220681476954	24.36101354822133	27.885694415154987	22.712071355146723
34	23.84681866463104	23.954210612902184	28.576487365801185	23.622483356665594
35	23.326611544057787	24.46549251417047	28.8738017982256	23.334094143546142
36	23.777303413028434	24.453701871438557	28.806042262498703	22.9629524530343
37	24.552937469208093	23.92396711888967	28.816457660515333	22.706637751386907
38	24.41595992497267	24.14209202780943	28.724534242860212	22.717413804357687
39	24.7818503208224	24.190816115791325	28.343532008107363	22.68380155527891
40	23.741642168221123	24.88890143034566	28.119069876421776	23.25038652501144
41	24.24340040075503	25.258509346492037	27.864982516359365	22.63310773639357
42	24.199459944967224	25.374767065449323	28.023310041232374	22.40246294835108
43	23.87972942000778	24.639399540414615	29.088098216093588	22.392772823484012
44	24.261040787578473	24.216870463797914	28.35502144450394	23.167067304119673
45	24.457078552987326	23.874168992527824	28.252068326437758	23.416684128047095
46	23.915580900395305	24.03230231857936	27.96939410720718	24.08272267381815
47	23.62898307232285	24.15506371643516	28.36143793209486	23.854515279147133
48	23.706024519067608	24.13101080526206	28.320402532146545	23.84256214352379
49	23.07265100771552	25.422734174351625	27.872817844319364	23.631796973613493
50	24.166236093531385	24.86652497022318	27.912240205077126	23.054998731168315
51	23.885912076364686	24.527033724369176	28.1850182108954	23.40203598837074
52	24.401513910600293	24.31606246591365	27.788844688620618	23.49357893486544
53	25.024151001394152	24.496346347336164	26.997464674937135	23.482037976332553
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18929.0
1	10785.5
2	2642.0
3	2170.0
4	1698.0
5	1805.5
6	1913.0
7	2189.0
8	2973.5
9	3482.0
10	4484.0
11	5486.0
12	7294.0
13	9102.0
14	11771.5
15	14441.0
16	20367.0
17	26293.0
18	35443.0
19	44593.0
20	63011.5
21	81430.0
22	479349.5
23	877269.0
24	599518.5
25	314892.0
26	308016.0
27	327087.5
28	346159.0
29	368072.0
30	389985.0
31	412908.0
32	435831.0
33	480150.0
34	524469.0
35	588578.5
36	652688.0
37	724435.5
38	796183.0
39	867670.0
40	939157.0
41	1017792.5
42	1176978.0
43	1257528.0
44	1359441.5
45	1461355.0
46	1548332.0
47	1635309.0
48	1753675.0
49	1872041.0
50	1889438.0
51	1906835.0
52	1898084.0
53	1889333.0
54	1858974.5
55	1828616.0
56	1804607.5
57	1780599.0
58	1585326.5
59	1219598.0
60	1049142.0
61	921513.0
62	793884.0
63	685620.0
64	577356.0
65	474933.5
66	372511.0
67	303930.0
68	235349.0
69	188550.0
70	141751.0
71	106704.5
72	71658.0
73	52031.5
74	32405.0
75	24572.0
76	12679.0
77	8619.0
78	6558.0
79	4497.0
80	3751.0
81	3005.0
82	2376.5
83	1748.0
84	1194.0
85	640.0
86	458.0
87	276.0
88	192.5
89	109.0
90	81.0
91	53.0
92	35.0
93	12.5
94	8.0
95	8.5
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.5231873E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.731829507546905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.48608409898307	31.501723918111097
2	11.89116288068936	9.924799639667976
3	4.0858748797618345	5.115331016141688
4	2.011543699873544	3.357815949204115
5	1.2691502710668603	2.6481981365809584
6	0.8435475725775713	2.112167008818736
7	0.6292545617423523	1.83819608612346
8	0.5197605522788651	1.7352447001960019
9	0.4132716214938256	1.5521922763639011
>10	2.567498436990732	19.093905225157616
>50	0.1708260693292556	4.94122067987395
>100	0.10351219956287326	7.813933387914609
>500	0.00584886822393483	1.7152438030043171
>1k	0.0023584803306575986	1.6030039457728025
>5k	1.624600193684189E-4	0.47353788839689553
>10k+	1.4334707591267227E-4	4.573486338672044
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	680062	2.6952497739664434	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	110281	0.4370702087791897	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	96601	0.38285306841866235	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	61238	0.2427009679384483	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	37909	0.15024251271397887	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	28373	0.1124490441117867	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0608854523007468E-4	0.0	0.0	0.0	7.926482508849026E-6
2	2.655371640464424E-4	0.0	0.0	0.0	2.7742688780971592E-5
3	2.734636465552914E-4	3.963241254424513E-6	0.0	0.0	4.7558895053094156E-5
4	2.734636465552914E-4	3.963241254424513E-6	0.0	0.0	5.152213630751867E-5
5	2.7742688780971593E-4	3.963241254424513E-6	0.0	0.0	7.530158383406574E-5
6	3.051695765906875E-4	7.926482508849026E-6	0.0	0.0	8.719130759733928E-5
7	3.09132817845112E-4	1.1889723763273539E-5	0.0	3.963241254424513E-6	9.11545488517638E-5
8	3.1309605909953653E-4	1.9816206272122567E-5	0.0	7.926482508849026E-6	9.908103136061282E-5
9	3.1309605909953653E-4	1.9816206272122567E-5	0.0	1.1889723763273539E-5	1.0700751386946185E-4
10	3.249857828628101E-4	1.9816206272122567E-5	0.0	1.1889723763273539E-5	1.1493399637831088E-4
11	3.4876523038935713E-4	2.3779447526547078E-5	0.0	1.5852965017698052E-5	1.4267668515928247E-4
12	3.8839764293360226E-4	2.3779447526547078E-5	0.0	1.5852965017698052E-5	1.6645613268582955E-4
13	5.310743280928848E-4	2.3779447526547078E-5	0.0	1.9816206272122567E-5	1.9023558021237662E-4
14	6.341186007079221E-4	2.3779447526547078E-5	0.0	1.9816206272122567E-5	2.2986799275662176E-4
15	7.609423208495065E-4	2.3779447526547078E-5	0.0	2.3779447526547078E-5	2.417577165198953E-4
16	8.560601109556948E-4	2.3779447526547078E-5	0.0	2.3779447526547078E-5	2.5364744028316883E-4
17	9.947735548605527E-4	2.3779447526547078E-5	0.0	2.7742688780971592E-5	2.9327985282741396E-4
18	0.0012127518238539009	2.3779447526547078E-5	0.0	2.7742688780971592E-5	3.1309605909953653E-4
19	0.0014148771278295511	2.7742688780971592E-5	0.0	2.7742688780971592E-5	3.2894902411723456E-4
20	0.001652671603095022	2.7742688780971592E-5	0.0	2.7742688780971592E-5	3.606549541526307E-4
21	0.0019459514559224359	2.7742688780971592E-5	0.0	3.1705930035396104E-5	3.606549541526307E-4
22	0.002215451861223303	2.7742688780971592E-5	0.0	3.963241254424513E-5	3.844344016791778E-4
23	0.0025087317140507167	2.7742688780971592E-5	0.0	3.963241254424513E-5	4.002873666968758E-4
24	0.00280993804938698	2.7742688780971592E-5	0.0	3.963241254424513E-5	4.1217709046014936E-4
25	0.003107181143468818	2.7742688780971592E-5	0.0	4.7558895053094156E-5	4.280300554778474E-4
26	0.003440093408840477	2.7742688780971592E-5	0.0	4.7558895053094156E-5	4.518095030043945E-4
27	0.003848307258046202	2.7742688780971592E-5	0.0	4.7558895053094156E-5	4.676624680220925E-4
28	0.004220851935962107	3.1705930035396104E-5	0.0	5.152213630751867E-5	5.033316393119132E-4
29	0.004656808473948803	3.1705930035396104E-5	0.0	5.152213630751867E-5	5.112581218207621E-4
30	0.00500557370433816	3.1705930035396104E-5	0.0	5.152213630751867E-5	5.310743280928848E-4
31	0.005433603759816007	3.1705930035396104E-5	0.0	5.5485377561943185E-5	5.469272931105828E-4
32	0.005810111678986336	3.1705930035396104E-5	0.0	5.944861881636769E-5	5.508905343650073E-4
33	0.006178693115647816	3.566917128982062E-5	0.0	5.944861881636769E-5	5.588170168738563E-4
34	0.006535384828546022	3.566917128982062E-5	0.0	5.944861881636769E-5	5.746699818915544E-4
35	0.007018900261585813	3.963241254424513E-5	0.0	6.737510132521672E-5	5.94486188163677E-4
36	0.00744693031706366	3.963241254424513E-5	0.0	7.133834257964124E-5	6.222288769446486E-4
37	0.007819474994979564	3.963241254424513E-5	0.0	7.133834257964124E-5	6.499715657256202E-4
38	0.008223725602930864	4.359565379866964E-5	0.0	7.133834257964124E-5	6.578980482344691E-4
39	0.008675535105935259	4.359565379866964E-5	0.0	7.133834257964124E-5	6.856407370154407E-4
40	0.009131307850194078	4.7558895053094156E-5	0.0	7.133834257964124E-5	7.054569432875633E-4
41	0.009587080594452896	4.7558895053094156E-5	0.0	7.133834257964124E-5	7.173466670508369E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	398125	0.0	45.614643	1
TCAACGC	403490	0.0	45.44844	12
GTATCAA	400705	0.0	45.44821	9
TGGTATC	400565	0.0	45.404255	7
GGTATCA	401325	0.0	45.394394	8
GTGGTAT	401100	0.0	45.350723	6
ACGCAGA	403930	0.0	45.3489	15
ATCAACG	404315	0.0	45.28828	11
CAACGCA	405050	0.0	45.287907	13
AGTGGTA	400980	0.0	45.275215	5
AACGCAG	405695	0.0	45.183464	14
CGCAGAG	405325	0.0	45.13832	16
AGAGTAC	393615	0.0	45.125328	19
TATCAAC	403750	0.0	45.09963	10
AGCAGTG	405640	0.0	44.83562	2
CAGAGTA	397985	0.0	44.815834	18
CAGTGGT	406875	0.0	44.707615	4
GCAGAGT	400395	0.0	44.706318	17
GCAGTGG	410200	0.0	44.630524	3
GTACATG	282585	0.0	44.582516	22
>>END_MODULE