FastQCFastQC Report
Thu 26 May 2016
SRR805357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18163356
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2898981.5960596709110364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG366940.20202213731867613No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG283220.15592933376409074No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC267320.14717544489025047No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG186920.1029104973772468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2066850.045.2490231
TCAACGC1811450.045.02809512
ACGCAGA2081700.045.00638615
AGTGGTA2067200.044.9776235
GTGGTAT2071500.044.9443866
AACGCAG2090700.044.84186614
GGTATCA1808450.044.823418
GTATCAA1812950.044.7601179
ATCAACG1821750.044.75416611
TGGTATC1808150.044.7528657
CAACGCA1824650.044.7319713
CGCAGAG2092250.044.72440716
AGAGTAC2063150.044.65719
TAAACGC274650.044.45020312
TATCAAC1830900.044.30460410
CAGAGTA2086300.044.22793218
CAGTGGT2106500.044.2154734
GCAGTGG2120400.044.1528243
GCAGAGT2103250.044.04357517
AGCAGTG2123900.044.0358052
Produced by FastQC (version 0.11.5)