##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805357_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18163356
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.25605488324955	35.0	35.0	37.0	33.0	37.0
2	35.40865399544005	36.0	35.0	37.0	33.0	37.0
3	37.05601029897779	39.0	37.0	39.0	33.0	39.0
4	37.03817158018595	39.0	37.0	39.0	33.0	39.0
5	37.04526674475796	39.0	37.0	39.0	33.0	39.0
6	37.0269786046147	39.0	37.0	39.0	33.0	39.0
7	36.804120009540085	39.0	37.0	39.0	33.0	39.0
8	37.720023491253485	40.0	37.0	40.0	33.0	40.0
9	37.62941512570695	40.0	37.0	40.0	33.0	40.0
10	37.30973879496719	39.0	36.0	40.0	32.0	40.0
11	37.40304726725612	39.0	37.0	40.0	32.0	40.0
12	37.37544152082908	39.0	37.0	40.0	32.0	40.0
13	32.82909848818687	39.0	34.0	40.0	3.0	40.0
14	34.73881363113733	39.0	34.0	40.0	18.0	40.0
15	36.20154133410147	38.0	34.0	40.0	29.0	40.0
16	36.717179688599394	38.0	36.0	40.0	31.0	40.0
17	36.836663995354165	39.0	36.0	40.0	31.0	40.0
18	36.85353785941321	39.0	36.0	40.0	31.0	40.0
19	36.85401882779812	39.0	36.0	40.0	31.0	40.0
20	36.64970036374335	39.0	36.0	40.0	31.0	40.0
21	36.49507078978136	38.0	36.0	40.0	30.0	40.0
22	36.40640441116719	38.0	36.0	40.0	30.0	40.0
23	36.1673909821511	38.0	35.0	40.0	30.0	40.0
24	36.14584435827828	38.0	35.0	40.0	30.0	40.0
25	36.091800105663296	38.0	35.0	40.0	30.0	40.0
26	35.98003860079602	38.0	35.0	40.0	30.0	40.0
27	35.895976382338155	38.0	35.0	40.0	30.0	40.0
28	35.67052035978373	38.0	34.0	40.0	29.0	40.0
29	35.52028265040888	38.0	34.0	40.0	28.0	40.0
30	30.927408128762107	37.0	30.0	39.0	3.0	40.0
31	32.362457026113454	37.0	30.0	39.0	15.0	40.0
32	33.58581861193493	36.0	30.0	39.0	24.0	40.0
33	34.161153588576916	37.0	33.0	39.0	25.0	40.0
34	34.31341669458001	37.0	33.0	39.0	25.0	40.0
35	34.353381720867006	37.0	33.0	39.0	25.0	40.0
36	34.32811298748976	37.0	33.0	39.0	25.0	40.0
37	34.166912601393705	37.0	33.0	39.0	25.0	40.0
38	34.04722816642475	36.0	32.0	39.0	25.0	40.0
39	33.91929806363978	36.0	32.0	38.0	24.0	40.0
40	33.78357320089966	36.0	32.0	38.0	24.0	40.0
41	33.64925920077766	36.0	32.0	38.0	24.0	40.0
42	33.34279017600051	36.0	31.0	38.0	24.0	40.0
43	33.26075082159927	36.0	31.0	38.0	24.0	40.0
44	32.97847154457579	35.0	31.0	38.0	23.0	40.0
45	32.721910147001466	35.0	30.0	38.0	23.0	40.0
46	32.51178713889658	35.0	30.0	38.0	22.0	39.0
47	32.27752784232165	35.0	30.0	38.0	22.0	39.0
48	32.2345395311307	34.0	30.0	38.0	22.0	39.0
49	31.972606273862606	34.0	29.0	38.0	21.0	39.0
50	31.592114750159606	34.0	29.0	37.0	20.0	39.0
51	31.46563300306397	34.0	28.0	37.0	20.0	39.0
52	31.143420742290136	34.0	28.0	37.0	19.0	39.0
53	32.25369133325361	35.0	30.0	38.0	22.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	24.0
13	65.0
14	200.0
15	533.0
16	1479.0
17	3517.0
18	7940.0
19	15512.0
20	27460.0
21	44712.0
22	67446.0
23	98017.0
24	135698.0
25	184206.0
26	240189.0
27	309637.0
28	394729.0
29	496593.0
30	622891.0
31	785653.0
32	997808.0
33	1284877.0
34	1676984.0
35	2108899.0
36	2345301.0
37	2865768.0
38	2910403.0
39	536810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.47277056068273	40.61624404652973	17.423602774729517	16.487382618058028
2	25.964794171297417	36.14658546581369	21.989273347942966	15.899347014945917
3	32.28734821912867	23.219266307393855	22.985889832253466	21.50749564122401
4	21.217626302099678	26.326115063758042	21.57384901776962	30.88240961637266
5	20.789786865378844	36.112709567549075	21.249393559207892	21.848110007864186
6	31.567294061736167	23.3558104570543	24.55544559056157	20.52144989064796
7	20.180323504092527	24.08506996174055	33.768071274933995	21.96653525923293
8	31.241886136020234	23.581121242131687	23.829968426539676	21.3470241953084
9	31.816466075982873	23.999430501720056	23.270947285292433	20.913156137004638
10	20.45459550536806	24.521866994183235	34.83947570041572	20.18406180003299
11	20.41413492088136	35.30931178136904	24.089022975709995	20.187530322039603
12	20.35407443426204	23.673048086487984	35.216245279781994	20.756632199467983
13	17.844306966179598	34.03917205608919	20.730651317961286	27.385869659769924
14	20.502406053154495	34.976212545743195	23.89527023530233	20.626111165799976
15	20.559554082406358	34.691111047980336	23.843468134413044	20.905866735200256
16	20.89874250111048	23.77632195283735	23.578869455622627	31.746066090429544
17	31.726190908772583	23.774015110423427	23.715931130788825	20.78386285001516
18	20.771789090077846	23.584892571615068	23.700531994197547	31.942786344109535
19	21.063695497682254	34.714526324320246	23.44410911727987	20.777669060717635
20	31.943903978978334	23.719179429175973	23.62887673401325	20.708039857832443
21	21.08118125306799	34.430993919846095	23.569983432577107	20.917841394508812
22	32.12271454680511	23.697905827535397	23.463890703898553	20.715488921760937
23	21.02517288104687	23.67985850192002	34.550338604826116	20.744630012206997
24	20.9748132448651	34.43892197014692	23.480974551178758	21.105290233809214
25	21.083587196110678	23.741482576237562	23.358172355373092	31.816757872278668
26	21.034620474322036	31.231227312838	26.925800496339992	20.808351716499967
27	21.17807964563377	23.430036827995885	34.41971296493886	20.97217056143149
28	28.879415235818755	23.64167723189481	26.64972816697531	20.829179365311123
29	28.80156068074644	23.62159283779936	26.768103868029673	20.808742613424524
30	24.888759544216388	33.57808986400971	23.28799259343923	18.24515799833467
31	26.153646936171924	24.698189035110033	27.256532328056554	21.891631700661485
32	24.184809238997463	25.78253159823548	27.889421976863748	22.14323718590331
33	23.866988016972194	25.06128272770737	28.332946840881167	22.73878241443927
34	23.431583898922646	25.14098165559272	28.46521865232394	22.96221579316069
35	22.89704611856972	25.34327907243573	28.86570631550689	22.893968493487655
36	23.056080605368305	25.14218737990931	28.94911050578979	22.8526215089326
37	23.79947296083389	25.10863080589292	28.561792215051007	22.530104018222183
38	23.621967217952452	25.245609897201817	28.671023130306978	22.461399754538753
39	23.675200772368278	25.083960254922054	28.54687757042256	22.693961402287112
40	23.382336392019184	25.63925411140981	28.139942860779694	22.838466635791317
41	23.377695179238902	25.910239275164788	28.284371016017083	22.427694529579227
42	23.59693880359995	25.677771222454705	28.329538880369903	22.395751093575438
43	23.50767115944873	25.5840275332378	28.47594354259202	22.432357764721452
44	23.601145074731782	25.213110396558875	28.587029841842003	22.59871468686734
45	23.62861246566989	24.843035615224412	28.55182159067961	22.97653032842609
46	23.525487250263662	25.1486454375502	28.091796471973574	23.234070840212567
47	23.15639796962632	25.15795539106319	28.48331002266321	23.202336616647276
48	23.149422386479678	25.00915029138888	28.566791291212922	23.274636030918515
49	23.014034410821434	25.932448827188104	28.016711228916062	23.036805533074396
50	23.478150183259086	25.668852165866262	28.141968918078796	22.711028732795853
51	23.39161331198926	25.246711015299155	28.33976826749418	23.021907405217405
52	23.805418998559517	25.320733679392728	27.87784922566072	22.995998096387034
53	24.105429635360338	25.46507374518233	27.455553918559982	22.973942700897346
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5920.0
1	3705.0
2	1490.0
3	1313.0
4	1136.0
5	1166.5
6	1197.0
7	1371.5
8	1884.5
9	2223.0
10	2970.0
11	3717.0
12	5269.0
13	6821.0
14	9394.5
15	11968.0
16	17244.5
17	22521.0
18	31483.0
19	40445.0
20	78100.0
21	115755.0
22	277882.0
23	440009.0
24	346482.5
25	257628.0
26	262300.0
27	282512.0
28	302724.0
29	330694.5
30	358665.0
31	385975.5
32	413286.0
33	456673.0
34	500060.0
35	558296.5
36	616533.0
37	673861.5
38	731190.0
39	788547.0
40	845904.0
41	904867.5
42	1019835.5
43	1075840.0
44	1133952.0
45	1192064.0
46	1226131.5
47	1260199.0
48	1295687.0
49	1331175.0
50	1331237.5
51	1331300.0
52	1307406.0
53	1283512.0
54	1231668.5
55	1179825.0
56	1122579.0
57	1065333.0
58	938700.5
59	705549.0
60	599030.0
61	514299.0
62	429568.0
63	361452.5
64	293337.0
65	237983.5
66	182630.0
67	145422.5
68	108215.0
69	84452.5
70	60690.0
71	45095.5
72	29501.0
73	21976.5
74	14452.0
75	10600.0
76	4871.0
77	2994.0
78	2226.0
79	1458.0
80	1117.0
81	776.0
82	554.5
83	333.0
84	203.0
85	73.0
86	46.0
87	19.0
88	15.5
89	12.0
90	7.5
91	3.0
92	3.5
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8163356E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.51302849382783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.42555936141031	44.64666368462012
2	10.427758770780358	11.577529395381864
3	3.489270885280249	5.81099982231739
4	1.673800305113622	3.7167089612300095
5	0.9457299065236171	2.6250165624155346
6	0.6089644398114405	2.0283276179388245
7	0.40991183643507906	1.5928813219184486
8	0.31138816685435733	1.3828880143381406
9	0.21561154504852637	1.077232485948938
>10	1.3360772430543166	13.694258572109321
>50	0.11093886343436753	4.24862035942652
>100	0.04314442932198578	3.962821796511769
>500	0.0011845866275301817	0.4299197608072796
>1k	5.480543616365716E-4	0.6600677156061661
>5k	4.0583979160423275E-5	0.15948559368408913
>10k+	7.102196353073996E-5	2.38657833574578
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	289898	1.5960596709110364	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	36694	0.20202213731867613	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	28322	0.15592933376409074	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	26732	0.14717544489025047	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	18692	0.1029104973772468	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3213417168060791E-4	0.0	0.0	0.0	0.0
2	1.486509431406839E-4	0.0	0.0	6.606708584030396E-5	5.505590486691997E-6
3	1.486509431406839E-4	0.0	0.0	7.157267632699595E-5	5.505590486691997E-6
4	1.486509431406839E-4	0.0	0.0	7.707826681368796E-5	5.505590486691997E-6
5	1.5415653362737592E-4	0.0	0.0	7.707826681368796E-5	1.651677146007599E-5
6	1.651677146007599E-4	5.505590486691997E-6	0.0	7.707826681368796E-5	1.651677146007599E-5
7	1.651677146007599E-4	1.1011180973383994E-5	0.0	7.707826681368796E-5	1.651677146007599E-5
8	1.651677146007599E-4	1.651677146007599E-5	0.0	9.359503827376395E-5	2.7527952433459983E-5
9	1.706733050874519E-4	1.651677146007599E-5	0.0	9.359503827376395E-5	2.7527952433459983E-5
10	1.706733050874519E-4	2.2022361946767988E-5	0.0	9.359503827376395E-5	3.853913340684398E-5
11	1.761788955741439E-4	2.2022361946767988E-5	0.0	9.359503827376395E-5	4.4044723893535976E-5
12	1.8168448606083589E-4	2.2022361946767988E-5	0.0	9.359503827376395E-5	4.955031438022797E-5
13	1.871900765475279E-4	2.2022361946767988E-5	0.0	9.359503827376395E-5	4.955031438022797E-5
14	2.037068480076039E-4	2.2022361946767988E-5	0.0	9.910062876045594E-5	5.5055904866919966E-5
15	2.0921243849429587E-4	2.2022361946767988E-5	0.0	9.910062876045594E-5	5.5055904866919966E-5
16	2.2572920995437187E-4	2.2022361946767988E-5	0.0	9.910062876045594E-5	6.056149535361196E-5
17	2.3674039092775586E-4	2.2022361946767988E-5	0.0	9.910062876045594E-5	6.606708584030396E-5
18	2.5876275287452385E-4	2.2022361946767988E-5	0.0	9.910062876045594E-5	6.606708584030396E-5
19	2.8078511482129183E-4	2.2022361946767988E-5	0.0	1.871900765475279E-4	8.258385730037996E-5
20	2.9179629579467584E-4	2.7527952433459983E-5	0.0	1.871900765475279E-4	9.910062876045594E-5
21	2.973018862813678E-4	2.7527952433459983E-5	0.0	2.1471802898098788E-4	9.910062876045594E-5
22	3.358410196882118E-4	2.7527952433459983E-5	0.0	2.2572920995437187E-4	9.910062876045594E-5
23	3.688745626083638E-4	2.7527952433459983E-5	0.0	2.2572920995437187E-4	9.910062876045594E-5
24	3.908969245551318E-4	2.7527952433459983E-5	0.0	2.2572920995437187E-4	9.910062876045594E-5
25	4.1291928650189977E-4	2.7527952433459983E-5	0.0	2.2572920995437187E-4	9.910062876045594E-5
26	4.459528294220517E-4	3.853913340684398E-5	0.0	2.2572920995437187E-4	1.0460621924714794E-4
27	4.844919628288957E-4	4.955031438022797E-5	0.0	2.2572920995437187E-4	1.0460621924714794E-4
28	5.340422772091237E-4	4.955031438022797E-5	0.0	2.2572920995437187E-4	1.1561740022053193E-4
29	5.780870011026597E-4	4.955031438022797E-5	0.0	2.2572920995437187E-4	1.1561740022053193E-4
30	6.386484964562716E-4	4.955031438022797E-5	0.0	2.2572920995437187E-4	1.2112299070722392E-4
31	7.267379442433435E-4	4.955031438022797E-5	0.0	2.2572920995437187E-4	1.2112299070722392E-4
32	7.983106205703396E-4	5.5055904866919966E-5	0.0	2.2572920995437187E-4	1.3213417168060791E-4
33	9.249392017642554E-4	5.5055904866919966E-5	0.0	2.3123480044106386E-4	1.431453526539919E-4
34	0.0010075230590646353	5.5055904866919966E-5	0.0	2.3123480044106386E-4	1.431453526539919E-4
35	0.0011286460497718592	5.5055904866919966E-5	0.0	2.3123480044106386E-4	1.651677146007599E-4
36	0.0012222410880456233	8.258385730037996E-5	0.0	2.3123480044106386E-4	1.706733050874519E-4
37	0.0013378584882661553	8.258385730037996E-5	0.0	2.3123480044106386E-4	1.761788955741439E-4
38	0.0014259479360532272	8.808944778707195E-5	0.0	2.4775157190113983E-4	1.8168448606083589E-4
39	0.0015085317933536072	8.808944778707195E-5	0.0	2.4775157190113983E-4	2.037068480076039E-4
40	0.0015966212411406791	8.808944778707195E-5	0.0	2.4775157190113983E-4	2.0921243849429587E-4
41	0.001767294546228131	8.808944778707195E-5	0.0	2.4775157190113983E-4	2.2572920995437187E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	206685	0.0	45.249023	1
TCAACGC	181145	0.0	45.028095	12
ACGCAGA	208170	0.0	45.006386	15
AGTGGTA	206720	0.0	44.977623	5
GTGGTAT	207150	0.0	44.944386	6
AACGCAG	209070	0.0	44.841866	14
GGTATCA	180845	0.0	44.82341	8
GTATCAA	181295	0.0	44.760117	9
ATCAACG	182175	0.0	44.754166	11
TGGTATC	180815	0.0	44.752865	7
CAACGCA	182465	0.0	44.73197	13
CGCAGAG	209225	0.0	44.724407	16
AGAGTAC	206315	0.0	44.657	19
TAAACGC	27465	0.0	44.450203	12
TATCAAC	183090	0.0	44.304604	10
CAGAGTA	208630	0.0	44.227932	18
CAGTGGT	210650	0.0	44.215473	4
GCAGTGG	212040	0.0	44.152824	3
GCAGAGT	210325	0.0	44.043575	17
AGCAGTG	212390	0.0	44.035805	2
>>END_MODULE