FastQCFastQC Report
Thu 26 May 2016
SRR805356_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805356_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13280566
Sequences flagged as poor quality0
Sequence length53
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6644275.003002131083871No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG328330.24722590889575038No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC316050.23797931503822956No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG303190.2282959928063307No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG266260.200488443037744No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA265370.1998182908770605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG190680.1435782179765531No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC171280.12897040683356417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTT157820.11883529662817083No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA143500.10805262366076869No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGG137610.10361757172096431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2478050.045.9862371
TGGTATC2850200.045.8844457
GTATCAA2861600.045.778039
GGTATCA2861600.045.771468
TCAACGC2874600.045.76311512
ACGCAGA2859850.045.66798815
CGCAGAG2853850.045.60836816
ATCAACG2882150.045.59757611
AGTGGTA2487200.045.5156635
GTGGTAT2495200.045.4733286
AGAGTAC2797200.045.47181319
TATCAAC2888800.045.33804710
CAACGCA2897700.045.32774413
CAGAGTA2815200.045.3196418
GCAGAGT2837250.045.2772117
GAGTACT1172850.045.26682720
CAGTGGT2507300.045.2370074
AACGCAG2916900.045.2364314
GCAGTGG2524550.045.133633
GTACATG1627950.045.10608322