##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805356_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13280566
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.326661152845446	35.0	33.0	35.0	32.0	37.0
2	35.08785905660948	35.0	35.0	37.0	33.0	37.0
3	37.283135297095015	39.0	37.0	39.0	34.0	39.0
4	37.302301874784554	39.0	37.0	39.0	34.0	39.0
5	37.34815556806841	39.0	37.0	39.0	34.0	39.0
6	32.46700788204358	39.0	35.0	39.0	3.0	39.0
7	34.18870671626495	38.0	34.0	39.0	17.0	39.0
8	36.89587077839905	39.0	35.0	40.0	32.0	40.0
9	37.618735827976	39.0	37.0	40.0	33.0	40.0
10	37.254354294839544	39.0	36.0	40.0	32.0	40.0
11	37.685514834232215	39.0	37.0	40.0	33.0	40.0
12	37.91008342566123	40.0	38.0	40.0	34.0	40.0
13	37.821649995941435	40.0	38.0	40.0	33.0	40.0
14	37.9616025401327	40.0	38.0	40.0	34.0	40.0
15	37.944380307285094	40.0	38.0	40.0	34.0	40.0
16	37.83721732944213	40.0	37.0	40.0	33.0	40.0
17	37.72361780363879	40.0	37.0	40.0	33.0	40.0
18	37.65878381990647	40.0	37.0	40.0	33.0	40.0
19	37.567361059762064	40.0	37.0	40.0	33.0	40.0
20	37.48259426593716	40.0	37.0	40.0	33.0	40.0
21	36.8921081375598	39.0	36.0	40.0	31.0	40.0
22	37.13365439394676	39.0	37.0	40.0	32.0	40.0
23	36.383849528702314	39.0	36.0	40.0	30.0	40.0
24	36.593262817262456	39.0	36.0	40.0	30.0	40.0
25	36.842015317720644	39.0	36.0	40.0	31.0	40.0
26	36.787495126337234	39.0	36.0	40.0	31.0	40.0
27	36.94304369256551	39.0	36.0	40.0	31.0	40.0
28	36.74743019235777	39.0	36.0	40.0	30.0	40.0
29	36.741420809926325	39.0	36.0	40.0	30.0	40.0
30	36.54908495616829	39.0	36.0	40.0	30.0	40.0
31	36.41314331030771	39.0	35.0	40.0	30.0	40.0
32	36.330203622345614	39.0	35.0	40.0	30.0	40.0
33	36.168005490127456	38.0	35.0	40.0	30.0	40.0
34	35.955769505606916	38.0	35.0	40.0	29.0	40.0
35	35.75428712902748	38.0	35.0	40.0	28.0	40.0
36	35.599686413967596	38.0	34.0	40.0	28.0	40.0
37	35.37958472553052	38.0	34.0	40.0	28.0	40.0
38	35.311103909276156	38.0	34.0	40.0	28.0	40.0
39	35.28377924555324	38.0	34.0	40.0	28.0	40.0
40	35.02968676184434	37.0	33.0	40.0	27.0	40.0
41	35.00988527145605	37.0	33.0	40.0	27.0	40.0
42	34.62077685544426	37.0	33.0	40.0	26.0	40.0
43	34.718125793735	37.0	33.0	40.0	26.0	40.0
44	34.67676942383329	37.0	33.0	40.0	26.0	40.0
45	29.839680853963603	35.0	27.0	39.0	3.0	40.0
46	31.073301695123536	35.0	27.0	39.0	12.0	40.0
47	32.29718853850054	35.0	28.0	39.0	20.0	40.0
48	33.23091877258846	35.0	31.0	39.0	23.0	40.0
49	33.185391270221466	35.0	31.0	39.0	23.0	40.0
50	33.51081941838925	36.0	32.0	39.0	23.0	40.0
51	33.441806847690074	36.0	32.0	39.0	23.0	40.0
52	33.18358675375733	35.0	31.0	39.0	22.0	40.0
53	32.79540231944934	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	7.0
12	16.0
13	44.0
14	117.0
15	330.0
16	777.0
17	1864.0
18	3877.0
19	7472.0
20	13040.0
21	21210.0
22	32203.0
23	46895.0
24	66295.0
25	90686.0
26	120946.0
27	156198.0
28	201999.0
29	259942.0
30	335147.0
31	437687.0
32	579101.0
33	776019.0
34	1037763.0
35	1358727.0
36	1678830.0
37	1954745.0
38	2318991.0
39	1779637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.653333750986215	50.036158097478676	18.827427987632454	17.483080163902653
2	21.189895069231234	48.75541449061734	15.543486625494726	14.511203814656696
3	41.6470954626482	21.874993882037856	18.94020179561624	17.537708859697695
4	18.593469585558324	21.116584940732196	19.382622698460292	40.90732277524919
5	17.553438610974865	43.137732232195525	21.3710319274043	17.93779722942531
6	34.706013282867616	30.520822681804376	18.49259286087656	16.28057117445145
7	19.50639754359867	21.183818520987735	41.53189705920666	17.77788687620693
8	38.99300677395828	21.852050582783896	21.16739602815121	17.987546615106613
9	39.43332686272558	21.11304593493982	21.33483618092783	18.118791021406768
10	18.27654785195149	21.79066012698555	41.96912240035553	17.96366962070743
11	17.938941758958165	42.7035112810704	21.344835754741176	18.012711205230257
12	17.98157548405693	21.3612582475777	42.35724591858509	18.299920349780272
13	18.196694327636337	21.72214648080511	21.159655394205338	38.92150379735321
14	18.085539426557574	42.886628476527285	21.04487865953906	17.982953437376086
15	18.04114372836218	42.8104946731939	20.94975470171979	18.198606896724133
16	18.159060389444246	21.549262282947883	21.11716473529818	39.174512592309696
17	39.360408283803565	21.331764022708068	21.15184699206344	18.155980701424923
18	18.20696497423378	21.223086425683967	21.090027337690277	39.47992126239198
19	18.399660074728743	42.528834990918305	20.876610228811032	18.194894705541916
20	39.61855993185833	21.508134517760762	20.9187921659363	17.95451338444461
21	18.912687908030428	42.054841638526554	21.0449614873342	17.987508966108823
22	39.530385979031315	21.29458940228903	21.08363453786533	18.091390080814325
23	18.51782521919623	21.334429571751688	42.06960004566071	18.07814516339138
24	18.503375533843965	42.062243431492305	21.06085689420165	18.373524140462084
25	18.198682194719712	21.556611367316727	20.903499143033514	39.34120729493005
26	18.545467113374535	33.21466871216182	30.066444457261838	18.17341971720181
27	18.659197205902217	21.165905127838677	41.917949882557714	18.25694778370139
28	30.73826070364772	21.240133891883826	29.799784135706265	18.221821268762188
29	30.805223211119166	21.31385815935857	29.81375944368636	18.067159185835905
30	30.80300945004904	20.976372543158174	29.97529623360932	18.245321773183463
31	25.595309717974366	23.107878082907007	31.092575421860786	20.204236777257837
32	23.257690974917786	24.879895932146265	31.952312875821708	19.91010021711424
33	22.90290940913211	24.258807945384255	32.200126109083	20.638156536400633
34	21.84744234545425	24.40431379204772	32.55288215878751	21.195361703710518
35	21.31608698002781	24.79542664070191	32.87203271306359	21.016453666206697
36	21.548343647401776	24.320424295169346	33.32315806419696	20.808073993231915
37	21.882004125426583	24.116366727141	33.242559089725546	20.75907005770688
38	21.839724300907054	24.136305636371223	32.914817034153515	21.10915302856821
39	22.451746409000943	24.035398792491225	32.6300776638586	20.88277713464923
40	21.70874343759144	24.552974624726083	32.70441184509757	21.03387009258491
41	21.983008856700835	24.976819512059954	32.420786885137275	20.619384746101936
42	22.2233977075977	24.66241273150557	32.292185438482065	20.82200412241466
43	21.734886901657653	24.84306015270735	32.98617694456697	20.435876001068028
44	21.902379763031185	24.43617237397864	32.74600645785729	20.915441405132885
45	18.650515347011563	35.38886821540588	27.962407626301474	17.998208811281085
46	21.457263191945284	24.46658523439438	32.480791857816904	21.595359715843436
47	21.750134745763095	24.406346837928446	32.436832887995884	21.406685528312575
48	21.739600556181117	24.121554759036627	32.59399486437551	21.54484982040675
49	21.5889292670207	25.297310370657396	32.01798778756869	21.095772574753212
50	21.8039954020032	24.906099634608946	32.26818796728995	21.021716996097908
51	21.723915983701296	24.73701045572907	32.16832776554855	21.370745795021083
52	22.156194246540394	24.70185382159164	31.7249505781606	21.417001353707366
53	23.007167013815526	24.84861714478133	30.669724468068605	21.47449137333454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15467.0
1	9404.0
2	3341.0
3	2529.0
4	1717.0
5	1732.0
6	1747.0
7	1869.0
8	2271.0
9	2551.0
10	3361.5
11	4172.0
12	5492.0
13	6812.0
14	9032.5
15	11253.0
16	16073.5
17	20894.0
18	30360.0
19	39826.0
20	104368.0
21	168910.0
22	519441.5
23	869973.0
24	570833.0
25	260311.5
26	248930.0
27	257620.5
28	266311.0
29	276229.0
30	286147.0
31	296963.0
32	307779.0
33	335355.0
34	362931.0
35	401389.0
36	439847.0
37	478208.5
38	516570.0
39	556116.5
40	595663.0
41	628535.0
42	689861.5
43	718316.0
44	764008.0
45	809700.0
46	829271.0
47	848842.0
48	883322.0
49	917802.0
50	902887.5
51	887973.0
52	860246.5
53	832520.0
54	787453.5
55	742387.0
56	713499.5
57	684612.0
58	608201.0
59	469524.5
60	407259.0
61	349507.0
62	291755.0
63	249352.5
64	206950.0
65	169818.0
66	132686.0
67	104764.0
68	76842.0
69	60210.5
70	43579.0
71	33481.0
72	23383.0
73	16429.0
74	9475.0
75	6810.5
76	3135.0
77	2124.0
78	1634.0
79	1144.0
80	920.5
81	697.0
82	521.5
83	346.0
84	249.5
85	153.0
86	116.5
87	80.0
88	59.0
89	38.0
90	28.5
91	19.0
92	15.5
93	6.5
94	1.0
95	1.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.3280566E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.7505278667557
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8027232020384	34.36962404895543
2	13.345479142024624	11.944344724807596
3	4.08629673847503	5.4859180820087925
4	1.7090382713222878	3.0592145914464006
5	0.8888943867221324	1.988924651180575
6	0.5450160174619354	1.463385268635513
7	0.35773893404002804	1.1206304295747227
8	0.25990382990889466	0.9304666866411617
9	0.1980680386328864	0.7977284354119165
>10	1.524471087146432	13.872126558082243
>50	0.15733369043554316	4.966337738823962
>100	0.11848042792861224	9.944550103293928
>500	0.0047674579562845465	1.3737917128116304
>1k	0.0014205018524987953	1.1148322838332165
>5k	1.5783173759350617E-4	0.4652904257743431
>10k+	2.104423167913416E-4	7.102834258718461
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	664427	5.003002131083871	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	32833	0.24722590889575038	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	31605	0.23797931503822956	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	30319	0.2282959928063307	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG	26626	0.200488443037744	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	26537	0.1998182908770605	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	19068	0.1435782179765531	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	17128	0.12897040683356417	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTT	15782	0.11883529662817083	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14350	0.10805262366076869	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGG	13761	0.10361757172096431	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.76489977912086E-5	0.0	0.0	0.0	0.0
2	3.76489977912086E-5	0.0	0.0	7.529799558241719E-6	0.0
3	3.76489977912086E-5	0.0	0.0	7.529799558241719E-6	0.0
4	4.5178797349450314E-5	0.0	0.0	7.529799558241719E-6	7.529799558241719E-6
5	4.5178797349450314E-5	0.0	0.0	7.529799558241719E-6	1.5059599116483439E-5
6	4.5178797349450314E-5	0.0	0.0	7.529799558241719E-6	1.5059599116483439E-5
7	4.5178797349450314E-5	0.0	0.0	7.529799558241719E-6	1.5059599116483439E-5
8	4.5178797349450314E-5	0.0	0.0	1.5059599116483439E-5	1.5059599116483439E-5
9	4.5178797349450314E-5	0.0	0.0	1.5059599116483439E-5	1.5059599116483439E-5
10	4.5178797349450314E-5	0.0	0.0	1.5059599116483439E-5	1.5059599116483439E-5
11	4.5178797349450314E-5	0.0	0.0	1.5059599116483439E-5	3.0119198232966877E-5
12	6.0238396465933754E-5	0.0	0.0	1.5059599116483439E-5	4.5178797349450314E-5
13	1.3553639204835096E-4	0.0	0.0	1.5059599116483439E-5	6.776819602417548E-5
14	1.505959911648344E-4	0.0	0.0	1.5059599116483439E-5	7.52979955824172E-5
15	2.0330458807252643E-4	0.0	0.0	1.5059599116483439E-5	7.52979955824172E-5
16	2.1083438763076814E-4	0.0	0.0	1.5059599116483439E-5	7.52979955824172E-5
17	2.334237863054933E-4	0.0	0.0	1.5059599116483439E-5	7.52979955824172E-5
18	2.4848338542197675E-4	0.0	0.0	2.2589398674725157E-5	9.035759469890063E-5
19	2.4848338542197675E-4	0.0	0.0	2.2589398674725157E-5	1.0541719381538407E-4
20	2.5601318498021846E-4	0.0	0.0	2.2589398674725157E-5	1.1294699337362579E-4
21	2.6354298453846016E-4	0.0	0.0	2.2589398674725157E-5	1.1294699337362579E-4
22	2.8613238321318534E-4	0.0	0.0	2.2589398674725157E-5	1.2800659249010923E-4
23	3.237813810043939E-4	0.0	0.0	2.2589398674725157E-5	1.505959911648344E-4
24	3.313111805626357E-4	0.0	0.0	2.2589398674725157E-5	1.6565559028131784E-4
25	3.614303787956025E-4	0.0	0.0	2.2589398674725157E-5	1.7318538983955955E-4
26	4.1413897570329456E-4	0.0	0.0	3.0119198232966877E-5	1.7318538983955955E-4
27	4.593177730527449E-4	0.0	0.0	3.0119198232966877E-5	1.8071518939780126E-4
28	4.8190717172747003E-4	0.0	0.0	3.0119198232966877E-5	1.88244988956043E-4
29	5.572051673098873E-4	0.0	0.0	3.0119198232966877E-5	2.0330458807252643E-4
30	6.099137642175793E-4	0.0	0.0	3.0119198232966877E-5	2.1083438763076814E-4
31	6.852117597999965E-4	0.0	0.0	3.76489977912086E-5	2.1083438763076814E-4
32	7.981587531736223E-4	0.0	0.0	3.76489977912086E-5	2.1083438763076814E-4
33	0.0010391123390373572	0.0	0.0	5.2708596907692034E-5	2.1836418718900987E-4
34	0.001310185123134059	0.0	0.0	1.581257907230761E-4	2.1836418718900987E-4
35	0.001686675101046145	0.0	0.0	1.581257907230761E-4	2.1836418718900987E-4
36	0.0017619730966285624	0.0	0.0	3.011919823296688E-4	2.334237863054933E-4
37	0.0019953968829340557	0.0	0.0	3.011919823296688E-4	2.334237863054933E-4
38	0.0021008140767494396	0.0	0.0	3.087217818879105E-4	2.334237863054933E-4
39	0.0021309332749824064	0.0	0.0	3.087217818879105E-4	2.4095358586373502E-4
40	0.002183641871890099	0.0	0.0	3.087217818879105E-4	2.4095358586373502E-4
41	0.0022438802683560325	0.0	0.0	3.087217818879105E-4	2.4848338542197675E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	247805	0.0	45.986237	1
TGGTATC	285020	0.0	45.884445	7
GTATCAA	286160	0.0	45.77803	9
GGTATCA	286160	0.0	45.77146	8
TCAACGC	287460	0.0	45.763115	12
ACGCAGA	285985	0.0	45.667988	15
CGCAGAG	285385	0.0	45.608368	16
ATCAACG	288215	0.0	45.597576	11
AGTGGTA	248720	0.0	45.515663	5
GTGGTAT	249520	0.0	45.473328	6
AGAGTAC	279720	0.0	45.471813	19
TATCAAC	288880	0.0	45.338047	10
CAACGCA	289770	0.0	45.327744	13
CAGAGTA	281520	0.0	45.31964	18
GCAGAGT	283725	0.0	45.27721	17
GAGTACT	117285	0.0	45.266827	20
CAGTGGT	250730	0.0	45.237007	4
AACGCAG	291690	0.0	45.23643	14
GCAGTGG	252455	0.0	45.13363	3
GTACATG	162795	0.0	45.106083	22
>>END_MODULE