FastQCFastQC Report
Thu 26 May 2016
SRR805355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25854222
Sequences flagged as poor quality0
Sequence length53
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9268193.584787815313104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG981290.37954729405510634No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG660020.25528519094482904No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG574110.22205657551791735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG568080.21972426785845656No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC392620.15185914316044785No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA392400.1517740506753597No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGT326200.1261689483442975No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTT318210.12307854399950616No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC313270.12116783092525468No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCCTAC275780.1066672978981924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6266250.046.2473221
ACGCAGA6311100.045.99872615
TCAACGC5476400.045.96154412
CGCAGAG6310600.045.95246516
TGGTATC5440250.045.936077
AGAGTAC6225300.045.89470719
TAAACGC851650.045.89074312
AAACGCA851500.045.87674713
GTATCAA5468250.045.8599
GGTATCA5467300.045.8312958
AGTGGTA6292850.045.826285
GTGGTAT6306650.045.816556
CAGAGTA6261800.045.7210118
AACGCAG6355200.045.71687314
GCAGAGT6285300.045.6913917
ATCAACG5510800.045.67292811
CAACGCA5519050.045.62722813
GCAGTGG6355400.045.6233673
CAGTGGT6330200.045.6041534
GTACATG4205600.045.5723222