##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805355_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25854222
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.42824572327104	36.0	35.0	37.0	33.0	37.0
2	35.50710502911285	37.0	35.0	37.0	33.0	37.0
3	37.16250355551213	39.0	37.0	39.0	33.0	39.0
4	37.07176588024966	39.0	37.0	39.0	33.0	39.0
5	37.048543754285085	39.0	37.0	39.0	33.0	39.0
6	37.09197855576548	39.0	37.0	39.0	33.0	39.0
7	36.74230990203457	39.0	37.0	39.0	32.0	39.0
8	37.692585760267704	40.0	37.0	40.0	33.0	40.0
9	37.68217902669823	40.0	37.0	40.0	33.0	40.0
10	37.103857234613365	39.0	36.0	40.0	32.0	40.0
11	37.3445125519538	39.0	36.0	40.0	32.0	40.0
12	37.49080641451907	39.0	37.0	40.0	33.0	40.0
13	32.872011348862095	39.0	34.0	40.0	3.0	40.0
14	34.847624268098265	39.0	34.0	40.0	18.0	40.0
15	36.378989744885764	39.0	34.0	40.0	30.0	40.0
16	36.887900707281	38.0	36.0	40.0	32.0	40.0
17	37.02078824882064	39.0	36.0	40.0	32.0	40.0
18	37.03780136954034	39.0	36.0	40.0	32.0	40.0
19	37.00256994776327	39.0	36.0	40.0	31.0	40.0
20	36.844839423131745	39.0	36.0	40.0	31.0	40.0
21	36.50497435196464	38.0	36.0	40.0	30.0	40.0
22	36.63512203151965	39.0	36.0	40.0	30.0	40.0
23	36.09755184278993	38.0	35.0	40.0	30.0	40.0
24	36.180862800667526	38.0	35.0	40.0	30.0	40.0
25	36.204273638556984	38.0	35.0	40.0	30.0	40.0
26	36.113180547455656	38.0	35.0	40.0	30.0	40.0
27	36.14299795986899	38.0	35.0	40.0	30.0	40.0
28	35.82897814523292	38.0	35.0	40.0	29.0	40.0
29	35.54854858908537	38.0	34.0	40.0	27.0	40.0
30	30.879221312480414	37.0	30.0	40.0	3.0	40.0
31	32.26566767315605	37.0	30.0	39.0	15.0	40.0
32	33.5071711305024	37.0	30.0	39.0	24.0	40.0
33	34.02139178661032	37.0	32.0	39.0	25.0	40.0
34	34.16889841048011	37.0	33.0	39.0	24.0	40.0
35	34.22557700633962	37.0	33.0	39.0	24.0	40.0
36	34.20980151713712	37.0	33.0	39.0	25.0	40.0
37	34.001395826182666	37.0	32.0	39.0	24.0	40.0
38	33.81893549146441	36.0	32.0	39.0	24.0	40.0
39	33.71435736878874	36.0	32.0	38.0	24.0	40.0
40	33.546654507724114	36.0	31.0	38.0	24.0	40.0
41	33.36731610024854	36.0	31.0	38.0	23.0	40.0
42	33.071097401422485	35.0	31.0	38.0	23.0	40.0
43	33.01767239408712	35.0	31.0	38.0	23.0	40.0
44	32.7770975278235	35.0	31.0	38.0	23.0	40.0
45	32.51423751989134	35.0	30.0	38.0	22.0	40.0
46	32.29388561759855	35.0	30.0	38.0	21.0	39.0
47	32.0490758143873	34.0	30.0	38.0	20.0	39.0
48	31.981468287848692	34.0	29.0	38.0	21.0	39.0
49	31.696711546764007	34.0	29.0	38.0	20.0	39.0
50	31.284111043836475	34.0	28.0	37.0	19.0	39.0
51	31.106637747598825	34.0	28.0	37.0	18.0	39.0
52	30.6847054612589	34.0	28.0	37.0	17.0	39.0
53	31.564568835217706	34.0	29.0	38.0	18.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	7.0
12	7.0
13	47.0
14	174.0
15	583.0
16	1734.0
17	4657.0
18	11187.0
19	22732.0
20	41701.0
21	68544.0
22	105546.0
23	152414.0
24	213798.0
25	288541.0
26	376793.0
27	476306.0
28	592097.0
29	727065.0
30	893803.0
31	1111446.0
32	1398298.0
33	1795522.0
34	2343530.0
35	2962551.0
36	3291754.0
37	4002894.0
38	4115135.0
39	855353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.319848572507809	45.50539559844423	26.07620140339168	16.098554425656282
2	20.927614839850914	50.97736068020148	12.087178643395264	16.00784583655234
3	44.432897652074	22.216429486835846	15.981664426026821	17.369008435063332
4	18.740343453382586	19.81170812256505	17.583460836686555	43.86448758736581
5	17.324419972877156	44.41336892674628	20.055219607845867	18.206991492530697
6	42.13293674046738	20.60405066530333	18.768365955858197	18.494646638371094
7	18.388137921922386	19.95797436875107	43.88726916632804	17.766618542998508
8	41.76148096817611	21.590036629220556	19.56704015305508	17.081442249548257
9	41.5991902599119	21.145204059901708	19.800920716160014	17.454684964026377
10	17.374663217481462	21.315826096024086	44.185243709905485	17.12426697658897
11	17.149094643033543	45.17249445757834	20.452709812733875	17.22570108665424
12	17.042535644661825	20.944494094620214	44.57554746764378	17.437422793074184
13	14.75360581339481	31.588508058761157	18.046162827873914	35.611723299970116
14	17.252764364752498	45.21001637566197	20.311228084913946	17.225991174671588
15	17.094910069233567	44.99832947980411	20.470231128981563	17.43652932198076
16	17.245860269939666	20.805735326323106	20.660528868360455	41.28787553537678
17	41.29060623058006	20.80212276354709	20.50238835266441	17.40488265320844
18	17.43608452035416	20.709576950333293	20.576055237709337	41.27828329160321
19	17.381992774719734	44.74251826258783	20.611090134524257	17.264398828168183
20	41.48973811704719	20.96617333911653	20.46470011745084	17.079388426385446
21	17.51324406512793	44.788800838795304	20.492819315932227	17.205135780144534
22	41.40050317507137	20.68786676311513	20.72864927051373	17.182980791299773
23	17.526421796795898	20.797686350801815	44.34027834989581	17.33561350250648
24	17.478371617602726	44.54566840185715	20.51045666738686	17.465503313153263
25	17.245106041094566	20.97483730123459	20.62684384778625	41.15321280988459
26	17.460490592213525	37.01994204273484	28.09184898311773	17.427718381933907
27	17.711389652336088	20.711294271395982	44.176413430657476	17.400902645610454
28	33.81274826216005	20.640025447294448	28.218942345277302	17.328283945268204
29	33.71259827505156	20.851112827916463	28.111718078385806	17.324570818646176
30	29.050876100622947	31.50821943124028	24.321192105490546	15.119712362646226
31	25.965677868782898	23.82261589615808	30.13221592976188	20.07949030529714
32	23.524107590628716	25.758868319456685	30.960970320437415	19.756053769477187
33	23.32412864715094	24.512000399779964	31.53295813735954	20.630912815709557
34	21.67681936048975	24.8772560241805	31.779378238494278	21.666546376835473
35	20.98624356207663	25.06337262826938	32.48924295614078	21.46114085351321
36	21.403827196966127	24.845017575852797	32.9423565713948	20.80879865578628
37	22.178400881681917	24.456322839650717	32.64558492612928	20.71969135253809
38	22.107228753586163	24.652851669642196	32.16909021667718	21.070829360094457
39	22.538821705793353	24.53888188938735	32.1533751818175	20.768921223001797
40	21.45266254772625	25.51871798733685	32.09310649533372	20.935512969603185
41	22.265249366235036	25.582216320413742	31.702957451204682	20.449576862146536
42	22.130250138642733	25.800907874930445	31.59986403768019	20.46897794874663
43	21.679356663681467	25.515136367282683	32.40263040984176	20.402876559194084
44	22.136786788633593	25.041898379305323	31.96958314970762	20.851731682353467
45	21.989128119964313	24.613879311471837	32.524776804345535	20.872215764218318
46	21.484920335255108	24.979908503918622	31.76644418076088	21.768726980065384
47	21.466064614127628	25.039144477060653	31.896233427561654	21.598557481250065
48	21.40084122430758	24.760168764699245	31.975539623663785	21.86345038732939
49	21.068141211133717	26.109952177249813	31.412157751256252	21.409748860360214
50	22.03065712052755	25.55441041699108	31.26118047566854	21.15375198681283
51	21.739478372236455	25.3603918153097	31.874747575076906	21.025382237376935
52	22.42532766988695	25.056097220794342	31.45318006474919	21.06539504456951
53	23.343340209579697	25.32804893529575	30.000508234206393	21.328102620918163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6260.0
1	3758.5
2	1257.0
3	1024.5
4	792.0
5	858.5
6	925.0
7	1058.5
8	1544.5
9	1897.0
10	2645.0
11	3393.0
12	5034.5
13	6676.0
14	10086.0
15	13496.0
16	21587.5
17	29679.0
18	43950.5
19	58222.0
20	158773.0
21	259324.0
22	739036.0
23	1218748.0
24	889287.5
25	540580.0
26	521333.0
27	543217.0
28	565101.0
29	577742.5
30	590384.0
31	606131.0
32	621878.0
33	681242.5
34	740607.0
35	837342.5
36	934078.0
37	1028233.5
38	1122389.0
39	1206277.5
40	1290166.0
41	1375469.0
42	1515295.5
43	1569819.0
44	1645205.0
45	1720591.0
46	1742383.0
47	1764175.0
48	1791134.5
49	1818094.0
50	1780170.5
51	1742247.0
52	1663661.0
53	1585075.0
54	1486740.5
55	1388406.0
56	1343447.0
57	1298488.0
58	1115772.0
59	816945.5
60	700835.0
61	596225.5
62	491616.0
63	416627.5
64	341639.0
65	280730.5
66	219822.0
67	173076.5
68	126331.0
69	99491.5
70	72652.0
71	56530.0
72	40408.0
73	28276.5
74	16145.0
75	11903.5
76	5814.5
77	3967.0
78	3114.0
79	2261.0
80	1906.0
81	1551.0
82	1137.5
83	724.0
84	453.0
85	182.0
86	117.0
87	52.0
88	33.5
89	15.0
90	12.0
91	9.0
92	5.5
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.5854222E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.0202372758186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.05858391163659	35.241978597562756
2	11.898025478656402	10.475078093683802
3	3.2371781811287375	4.275040549121697
4	1.3044151363104668	2.2968265522622406
5	0.6852052892801923	1.5081449708379995
6	0.4065109700849706	1.0736825615018164
7	0.30956907391533867	0.9539112860906043
8	0.23763294582831185	0.8368526927931228
9	0.16348529209818152	0.6476995214341618
>10	1.439361386871722	13.685156272582976
>50	0.11479430323462908	3.5481083961517155
>100	0.12627664586254736	11.842496411589897
>500	0.015305255521264743	4.526606251621003
>1k	0.003268194553228506	2.250274068785118
>5k	2.1244060465057198E-4	0.6327431947346942
>10k+	1.7549441253737579E-4	6.205400579246385
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	926819	3.584787815313104	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	98129	0.37954729405510634	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	66002	0.25528519094482904	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG	57411	0.22205657551791735	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	56808	0.21972426785845656	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	39262	0.15185914316044785	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	39240	0.1517740506753597	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGT	32620	0.1261689483442975	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTT	31821	0.12307854399950616	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	31327	0.12116783092525468	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCCTAC	27578	0.1066672978981924	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0886337248902714E-4	3.86784023127828E-6	0.0	0.0	7.73568046255656E-6
2	2.0886337248902714E-4	3.86784023127828E-6	0.0	1.353744080947398E-4	1.1603520693834841E-5
3	2.2046689318286198E-4	3.86784023127828E-6	0.0	1.392422483260181E-4	1.9339201156391402E-5
4	2.3207041387669682E-4	7.73568046255656E-6	0.0	2.591452954956448E-4	1.9339201156391402E-5
5	2.3207041387669682E-4	7.73568046255656E-6	0.0	2.6301313572692307E-4	2.3207041387669682E-5
6	2.591452954956448E-4	7.73568046255656E-6	0.0	2.6301313572692307E-4	2.3207041387669682E-5
7	2.591452954956448E-4	7.73568046255656E-6	0.0	2.746166564207579E-4	2.3207041387669682E-5
8	2.6301313572692307E-4	7.73568046255656E-6	0.0	2.784844966520362E-4	3.481056208150452E-5
9	2.6688097595820135E-4	7.73568046255656E-6	0.0	2.784844966520362E-4	3.481056208150452E-5
10	2.707488161894796E-4	7.73568046255656E-6	0.0	2.784844966520362E-4	4.2546242544061084E-5
11	2.746166564207579E-4	1.1603520693834841E-5	0.0	2.8235233688331447E-4	6.575328393173077E-5
12	2.784844966520362E-4	1.1603520693834841E-5	0.0	2.8622017711459275E-4	6.962112416300905E-5
13	3.0555937827098415E-4	1.1603520693834841E-5	0.0	2.8622017711459275E-4	6.962112416300905E-5
14	3.2876641965865383E-4	1.547136092511312E-5	0.0	2.9008801734587103E-4	7.735680462556561E-5
15	3.597091415088801E-4	1.547136092511312E-5	0.0	2.9008801734587103E-4	7.735680462556561E-5
16	4.0225538405294116E-4	1.9339201156391402E-5	0.0	3.0555937827098415E-4	8.122464485684389E-5
17	4.4480162659700224E-4	1.9339201156391402E-5	0.0	3.0555937827098415E-4	8.509248508812217E-5
18	5.028192300661764E-4	2.3207041387669682E-5	0.0	3.0555937827098415E-4	9.282816555067873E-5
19	5.492333128415158E-4	2.7074881618947963E-5	0.0	4.4480162659700224E-4	1.0443168624451357E-4
20	6.0725091631069E-4	2.7074881618947963E-5	0.0	4.4480162659700224E-4	1.1216736670707013E-4
21	6.807398807049773E-4	3.481056208150452E-5	0.0	5.647046737666289E-4	1.1216736670707013E-4
22	7.774358864869344E-4	4.2546242544061084E-5	0.0	5.647046737666289E-4	1.1603520693834841E-4
23	9.089424543503959E-4	4.2546242544061084E-5	0.0	5.685725139979072E-4	1.2377088740090497E-4
24	9.51488696894457E-4	5.0281923006617645E-5	0.0	5.685725139979072E-4	1.2763872763218325E-4
25	0.0010327133417513009	5.0281923006617645E-5	0.0	5.685725139979072E-4	1.353744080947398E-4
26	0.0011139379866081447	5.0281923006617645E-5	0.0	5.724403542291855E-4	1.353744080947398E-4
27	0.001171955590077319	1.1990304716962669E-4	0.0	5.724403542291855E-4	1.392422483260181E-4
28	0.0012609159153967194	1.1990304716962669E-4	0.0	5.763081944604638E-4	1.392422483260181E-4
29	0.001392422483260181	1.2377088740090497E-4	0.0	5.763081944604638E-4	1.5084576901985293E-4
30	0.0015548717729738687	1.2377088740090497E-4	0.0	5.801760346917421E-4	1.585814494824095E-4
31	0.0017637351454628959	1.2377088740090497E-4	0.0	5.840438749230203E-4	1.585814494824095E-4
32	0.0020190126007272624	1.2377088740090497E-4	0.0	5.917795553855769E-4	1.585814494824095E-4
33	0.002185329730672228	1.2763872763218325E-4	0.0	5.995152358481335E-4	1.6244928971368778E-4
34	0.0023400433399233594	1.353744080947398E-4	0.0	6.033830760794117E-4	1.6631712994496606E-4
35	0.0024754177480180994	1.353744080947398E-4	0.0	6.033830760794117E-4	1.6631712994496606E-4
36	0.00267654544004457	1.353744080947398E-4	0.0	6.149865967732466E-4	1.7018497017624434E-4
37	0.002854466090683371	1.392422483260181E-4	0.0	6.227222772358031E-4	1.779206506388009E-4
38	0.0030439902620160064	1.4311008855729637E-4	0.0	6.381936381609163E-4	1.8565633110135746E-4
39	0.0033186069184367646	1.4311008855729637E-4	0.0	6.652685197798642E-4	1.8565633110135746E-4
40	0.0035545451725447394	1.4311008855729637E-4	0.0	6.652685197798642E-4	1.8565633110135746E-4
41	0.003794351266883993	1.4697792878857465E-4	0.0	6.807398807049773E-4	1.8952417133263574E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	626625	0.0	46.247322	1
ACGCAGA	631110	0.0	45.998726	15
TCAACGC	547640	0.0	45.961544	12
CGCAGAG	631060	0.0	45.952465	16
TGGTATC	544025	0.0	45.93607	7
AGAGTAC	622530	0.0	45.894707	19
TAAACGC	85165	0.0	45.890743	12
AAACGCA	85150	0.0	45.876747	13
GTATCAA	546825	0.0	45.859	9
GGTATCA	546730	0.0	45.831295	8
AGTGGTA	629285	0.0	45.82628	5
GTGGTAT	630665	0.0	45.81655	6
CAGAGTA	626180	0.0	45.72101	18
AACGCAG	635520	0.0	45.716873	14
GCAGAGT	628530	0.0	45.69139	17
ATCAACG	551080	0.0	45.672928	11
CAACGCA	551905	0.0	45.627228	13
GCAGTGG	635540	0.0	45.623367	3
CAGTGGT	633020	0.0	45.604153	4
GTACATG	420560	0.0	45.57232	22
>>END_MODULE