FastQCFastQC Report
Thu 26 May 2016
SRR805354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20214784
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT995450.49243662460108406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC634750.042.26482812
ACGCAGA732650.042.08605615
AAGCAGT736200.041.8065031
CGCAGAG743000.041.4713316
GGTATCA645300.041.413618
GTATCAA647000.041.3483779
AGAGTAC737600.041.1600419
ATCAACG653000.041.1016111
TGGTATC650200.041.0219887
AACGCAG765200.040.360314
GTGGTAT765300.040.2260556
AGTGGTA765150.040.181735
CAACGCA671250.040.0051413
GCAGAGT768300.039.79981617
CAGAGTA768000.039.65012718
TAAACGC103700.039.36306412
GTACATG518400.039.2754622
GAGTACA525900.039.1175120
TATCAAC686500.039.0000710
CAGTGGT791100.038.9082264
Produced by FastQC (version 0.11.5)