##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20214784
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.40954204605897	35.0	35.0	37.0	33.0	37.0
2	35.46884082461628	37.0	35.0	37.0	33.0	37.0
3	37.06134525107961	39.0	37.0	39.0	33.0	39.0
4	37.05702826208778	39.0	37.0	39.0	33.0	39.0
5	37.05597457781394	39.0	37.0	39.0	33.0	39.0
6	36.97155121716858	39.0	37.0	39.0	33.0	39.0
7	36.817726719216985	39.0	37.0	39.0	33.0	39.0
8	37.68068286062319	40.0	37.0	40.0	33.0	40.0
9	37.57905046128616	40.0	37.0	40.0	33.0	40.0
10	37.45361518579669	39.0	37.0	40.0	33.0	40.0
11	37.466365359135175	39.0	37.0	40.0	33.0	40.0
12	37.32808562287878	39.0	36.0	40.0	32.0	40.0
13	32.756122746599715	39.0	34.0	40.0	3.0	40.0
14	34.65855158284155	38.0	34.0	40.0	18.0	40.0
15	36.10990565123031	38.0	34.0	40.0	29.0	40.0
16	36.654963763154726	38.0	36.0	40.0	31.0	40.0
17	36.763187526515246	38.0	36.0	40.0	31.0	40.0
18	36.78145786766755	39.0	36.0	40.0	31.0	40.0
19	36.81116691625298	39.0	36.0	40.0	31.0	40.0
20	36.543505584823464	38.0	36.0	40.0	31.0	40.0
21	36.49863347538118	38.0	36.0	40.0	30.0	40.0
22	36.27357566620549	38.0	35.0	40.0	30.0	40.0
23	36.21302206345613	38.0	35.0	40.0	30.0	40.0
24	36.12455596854263	38.0	35.0	40.0	30.0	40.0
25	36.0240151465383	38.0	35.0	40.0	30.0	40.0
26	35.88609925290322	38.0	35.0	40.0	29.0	40.0
27	35.728185173781725	38.0	34.0	40.0	29.0	40.0
28	35.55757959125361	38.0	34.0	40.0	28.0	40.0
29	35.491135794475966	38.0	34.0	40.0	28.0	40.0
30	30.887712676029583	37.0	30.0	39.0	3.0	40.0
31	32.33222996594967	37.0	30.0	39.0	15.0	40.0
32	33.58045631355744	36.0	30.0	39.0	24.0	40.0
33	34.2090531365559	37.0	33.0	39.0	25.0	40.0
34	34.3365343898802	37.0	33.0	39.0	25.0	40.0
35	34.36602839783002	37.0	33.0	39.0	25.0	40.0
36	34.31738479124981	37.0	33.0	39.0	25.0	40.0
37	34.17375644478813	37.0	33.0	39.0	25.0	40.0
38	34.08110737171369	37.0	32.0	39.0	25.0	40.0
39	33.948932771183706	36.0	32.0	39.0	24.0	40.0
40	33.81582959283661	36.0	32.0	38.0	24.0	40.0
41	33.65727217268312	36.0	32.0	38.0	24.0	40.0
42	33.385282128169166	36.0	31.0	38.0	24.0	40.0
43	33.267127217387035	36.0	31.0	38.0	24.0	40.0
44	32.983030241629095	35.0	31.0	38.0	23.0	40.0
45	32.71239183164163	35.0	30.0	38.0	23.0	40.0
46	32.489720493674334	35.0	30.0	38.0	22.0	39.0
47	32.255348362861554	35.0	30.0	38.0	22.0	39.0
48	32.20323289133339	34.0	30.0	38.0	22.0	39.0
49	31.936720075762373	34.0	29.0	38.0	21.0	39.0
50	31.56450269268274	34.0	29.0	37.0	20.0	39.0
51	31.46754271527215	34.0	29.0	37.0	20.0	39.0
52	31.187714001791957	34.0	28.0	37.0	19.0	39.0
53	32.26281784658199	34.0	30.0	38.0	22.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	6.0
12	23.0
13	83.0
14	260.0
15	768.0
16	2154.0
17	5013.0
18	10810.0
19	20472.0
20	34733.0
21	54708.0
22	81057.0
23	115875.0
24	157406.0
25	209568.0
26	272195.0
27	348308.0
28	442217.0
29	555961.0
30	694429.0
31	871869.0
32	1104433.0
33	1418426.0
34	1840580.0
35	2309461.0
36	2572705.0
37	3153603.0
38	3276619.0
39	661041.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.2176229041082	28.87655885910035	23.03572969169495	22.8700885450965
2	25.22558242521909	25.752167324666935	26.480371989134287	22.541878260979686
3	25.030146253355962	24.365296210931565	25.475033520021782	25.129524015690695
4	23.21030489368573	26.551854326021985	24.714377358669772	25.523463421622512
5	25.169148480636743	28.950445377007245	23.569235268603414	22.311170873752594
6	27.0543677340307	24.788461751557673	25.36081018723722	22.79636032717441
7	23.597452240894583	25.448132416354284	28.281103572514056	22.67331177023707
8	26.423477985221112	24.830574494389847	25.40602462039664	23.339922899992402
9	26.06653625386252	24.991936594524088	25.73103427669571	23.210492874917684
10	22.85318507484423	25.386370687908418	28.913744514905527	22.846699722341825
11	22.805863273137124	28.448505806443443	25.7342447982625	23.01138612215693
12	22.764106705270756	25.06386415011904	28.905295253216657	23.266733891393546
13	19.946515382009522	35.20716323261233	22.045573180499975	22.800748204878172
14	22.966879092054608	28.41468897218986	25.49008685920166	23.12834507655387
15	23.012608000164633	28.267004980117523	25.449690681829694	23.270696337888154
16	23.22563031096449	25.135668033850866	25.274210201800823	26.36449145338382
17	26.45668140703358	25.021558479180385	25.39580437762778	23.12595573615825
18	23.120924764766222	24.95984127260524	25.368176083405096	26.551057879223443
19	23.40481105313814	28.23459305822907	25.1966531029963	23.163942785636493
20	26.54346937370194	24.955057644939465	25.34285797958563	23.158615001772958
21	23.329460260371814	28.087804450445773	25.264341187123247	23.318394102059166
22	26.705519089395167	24.914453698837445	25.218132432184287	23.1618947795831
23	23.29085979845246	24.965663743921283	28.616956777772153	23.12651967985411
24	23.314026011853503	28.147849613431436	25.10908847702751	23.42903589768755
25	23.40483084063624	24.96685594068183	25.09623155013677	26.53208166854516
26	23.40050232542678	27.063435355035203	26.298945365926247	23.23711695361177
27	23.479231833493746	24.832879737918546	28.397810236310217	23.290078192277495
28	25.775872747391215	24.84750269901474	26.154466948546172	23.22215760504787
29	25.70640873530976	24.94995247042956	26.14557741502457	23.19806137923611
30	22.21028926156223	34.83450033401297	22.681300972595107	20.273909431829694
31	24.98217146421154	25.249930941631632	26.221437735866974	23.546459858289854
32	24.33156842041943	25.60628894179626	26.428736512841294	23.63340612494301
33	24.14956301289195	25.307141545514412	26.583806188579608	23.959489253014034
34	24.064644964794084	25.376224648257434	26.628065874955674	23.931064511992805
35	23.969249436452053	25.552214656362395	26.69155900948533	23.786976897700217
36	23.902768389709234	25.382343932044982	26.718455166278304	23.996432511967477
37	24.142894626032117	25.40220068638873	26.60398448976749	23.85092019781166
38	24.12171705619016	25.474167816979886	26.611845073387876	23.792270053442074
39	24.22643249613748	25.336224220847477	26.496404809470135	23.940938473544907
40	24.185046943860495	25.440078904627427	26.445021623777926	23.92985252773416
41	24.086144081480168	25.659116614849804	26.510018608163215	23.744720695506814
42	24.26417220188947	25.437585679866775	26.549108810660556	23.749133307583204
43	24.20211366097209	25.50248372676156	26.411288886391265	23.884113725875082
44	24.327798902031304	25.34787905722861	26.555871188136365	23.768450852603717
45	24.140767469986322	25.260215493769312	26.59481793127248	24.004199104971885
46	24.12945396794742	25.52668878381288	26.365080131452306	23.9787771167874
47	24.045347207271668	25.47172406096449	26.56319750930804	23.919731222455802
48	24.14702031938605	25.335032024086924	26.532967159085153	23.98498049744187
49	24.114598503748542	25.69084092117927	26.267023184615773	23.92753739045641
50	24.192932261853503	25.575583691618963	26.45761636631883	23.773867680208703
51	24.06232488064181	25.412653432260267	26.538408721062762	23.986612966035157
52	24.31706418431184	25.474271701344918	26.268165912631076	23.940498201712174
53	24.373552544513956	25.621426377843065	26.042227312446176	23.9627937651968
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8627.0
1	5482.0
2	2337.0
3	2119.0
4	1901.0
5	1932.0
6	1963.0
7	2231.5
8	3049.0
9	3598.0
10	4572.0
11	5546.0
12	7471.5
13	9397.0
14	12566.0
15	15735.0
16	22186.5
17	28638.0
18	39233.5
19	49829.0
20	73447.0
21	97065.0
22	166313.0
23	235561.0
24	219369.5
25	227487.5
26	251797.0
27	282214.5
28	312632.0
29	338615.5
30	364599.0
31	390305.5
32	416012.0
33	461292.5
34	506573.0
35	565856.5
36	625140.0
37	685090.5
38	745041.0
39	806172.0
40	867303.0
41	927619.5
42	1045236.0
43	1102536.0
44	1177215.0
45	1251894.0
46	1305001.5
47	1358109.0
48	1429043.0
49	1499977.0
50	1526331.5
51	1552686.0
52	1528272.0
53	1503858.0
54	1442244.0
55	1380630.0
56	1293371.0
57	1206112.0
58	1117868.5
59	935825.5
60	842026.0
61	729050.0
62	616074.0
63	525969.0
64	435864.0
65	359354.0
66	282844.0
67	232949.0
68	183054.0
69	146892.0
70	110730.0
71	84376.0
72	58022.0
73	42575.5
74	27129.0
75	20511.5
76	10696.0
77	7498.0
78	5905.5
79	4313.0
80	3642.0
81	2971.0
82	2237.5
83	1504.0
84	938.5
85	373.0
86	234.5
87	96.0
88	66.0
89	36.0
90	25.0
91	14.0
92	9.5
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0214784E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.003117317334116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3088853793037	37.88376379914356
2	11.865546543125477	11.62897538574132
3	3.9539917811248237	5.812737693667039
4	1.8692362516186667	3.663936133275336
5	1.094456030225972	2.6815878623913543
6	0.7331773124344426	2.155678431337963
7	0.5143945373709102	1.7644855103527768
8	0.3875787369872561	1.5194053054632555
9	0.3015689317160741	1.330003596613131
>10	1.7693532150029543	15.759425537550861
>50	0.11301387545208112	3.8133885316962006
>100	0.07981880778009877	8.089742987413898
>500	0.008033180505853185	2.5361732277493827
>1k	8.727909158447529E-4	0.6109582889911715
>5k	5.187602553167225E-5	0.190534502207091
>10k+	2.075041021266833E-5	0.5592032064055749
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99545	0.49243662460108406	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8691872245580264E-4	0.0	0.0	0.0	2.4734372625500226E-5
2	2.8691872245580264E-4	0.0	0.0	8.409686692670077E-5	4.452187072590041E-5
3	2.9681247150600273E-4	0.0	0.0	8.904374145180082E-5	6.430936882630059E-5
4	2.9681247150600273E-4	0.0	0.0	1.0883123955220101E-4	9.89374905020009E-5
5	3.017593460311028E-4	0.0	0.0	1.187249886024011E-4	1.0883123955220101E-4
6	3.165999696064029E-4	0.0	0.0	1.187249886024011E-4	1.0883123955220101E-4
7	3.165999696064029E-4	0.0	0.0	1.2367186312750114E-4	1.187249886024011E-4
8	3.165999696064029E-4	0.0	0.0	1.2861873765260119E-4	1.3356561217770123E-4
9	3.3144059318170304E-4	4.9468745251000454E-6	0.0	1.3356561217770123E-4	1.3356561217770123E-4
10	3.512280912821032E-4	4.9468745251000454E-6	0.0	1.3356561217770123E-4	1.4840623575300136E-4
11	3.7596246390760344E-4	4.9468745251000454E-6	0.0	1.3356561217770123E-4	1.632468593283015E-4
12	3.908030874829036E-4	4.9468745251000454E-6	0.0	1.3356561217770123E-4	1.8303435742870168E-4
13	4.105905855833038E-4	4.9468745251000454E-6	0.0	1.3356561217770123E-4	1.978749810040018E-4
14	4.2543120915860394E-4	4.9468745251000454E-6	0.0	1.3356561217770123E-4	2.275562281546021E-4
15	4.452187072590041E-4	4.9468745251000454E-6	0.0	1.3851248670280128E-4	2.5229060078010233E-4
16	4.650062053594043E-4	4.9468745251000454E-6	0.0	1.4345936122790132E-4	2.7702497340560255E-4
17	4.7984682893470443E-4	9.893749050200091E-6	0.0	1.4840623575300136E-4	3.067062205562028E-4
18	5.045812015602047E-4	9.893749050200091E-6	0.0	1.4840623575300136E-4	3.165999696064029E-4
19	5.144749506104047E-4	9.893749050200091E-6	0.0	3.165999696064029E-4	3.512280912821032E-4
20	5.688905703865052E-4	9.893749050200091E-6	0.0	3.2154684413150295E-4	3.512280912821032E-4
21	5.738374449116053E-4	9.893749050200091E-6	0.0	3.908030874829036E-4	3.710155893825034E-4
22	5.837311939618054E-4	9.893749050200091E-6	0.0	3.908030874829036E-4	4.0564371105820376E-4
23	6.084655665873056E-4	1.4840623575300137E-5	0.0	3.957499620080036E-4	4.204843346335039E-4
24	6.381468137379059E-4	1.4840623575300137E-5	0.0	3.957499620080036E-4	4.452187072590041E-4
25	6.727749354136062E-4	1.4840623575300137E-5	0.0	3.957499620080036E-4	4.6005933083430425E-4
26	7.024561825642064E-4	1.4840623575300137E-5	0.0	3.957499620080036E-4	4.946874525100046E-4
27	7.469780532901068E-4	1.4840623575300137E-5	0.0	3.957499620080036E-4	4.996343270351046E-4
28	8.261280456917077E-4	1.4840623575300137E-5	0.0	4.0564371105820376E-4	5.39209323235905E-4
29	9.003311635682083E-4	1.4840623575300137E-5	0.0	4.0564371105820376E-4	5.44156197761005E-4
30	9.94321779545109E-4	1.4840623575300137E-5	0.0	4.0564371105820376E-4	5.491030722861051E-4
31	0.0010487373993212097	1.4840623575300137E-5	0.0	4.105905855833038E-4	5.639436958614052E-4
32	0.0011130467681475104	1.4840623575300137E-5	0.0	4.105905855833038E-4	5.886780684869054E-4
33	0.0012367186312750114	1.4840623575300137E-5	0.0	4.105905855833038E-4	6.430936882630059E-4
34	0.0012762936274758117	1.4840623575300137E-5	0.0	4.105905855833038E-4	6.678280608885061E-4
35	0.0013702842434527127	1.4840623575300137E-5	0.0	4.1553746010840385E-4	7.074030570893065E-4
36	0.0014395404868041133	1.4840623575300137E-5	0.0	4.204843346335039E-4	7.222436806646066E-4
37	0.0015384779773061142	1.4840623575300137E-5	0.0	4.2543120915860394E-4	7.370843042399068E-4
38	0.0016868842130591155	1.4840623575300137E-5	0.0	4.30378083683704E-4	7.61818676865407E-4
39	0.0017759279545109163	1.9787498100400182E-5	0.0	4.30378083683704E-4	7.914999240160072E-4
40	0.0019342279393141178	1.9787498100400182E-5	0.0	4.30378083683704E-4	8.063405475913075E-4
41	0.002077687300542019	1.9787498100400182E-5	0.0	4.402718327339041E-4	8.261280456917077E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	63475	0.0	42.264828	12
ACGCAGA	73265	0.0	42.086056	15
AAGCAGT	73620	0.0	41.806503	1
CGCAGAG	74300	0.0	41.47133	16
GGTATCA	64530	0.0	41.41361	8
GTATCAA	64700	0.0	41.348377	9
AGAGTAC	73760	0.0	41.16004	19
ATCAACG	65300	0.0	41.10161	11
TGGTATC	65020	0.0	41.021988	7
AACGCAG	76520	0.0	40.3603	14
GTGGTAT	76530	0.0	40.226055	6
AGTGGTA	76515	0.0	40.18173	5
CAACGCA	67125	0.0	40.00514	13
GCAGAGT	76830	0.0	39.799816	17
CAGAGTA	76800	0.0	39.650127	18
TAAACGC	10370	0.0	39.363064	12
GTACATG	51840	0.0	39.27546	22
GAGTACA	52590	0.0	39.11751	20
TATCAAC	68650	0.0	39.00007	10
CAGTGGT	79110	0.0	38.908226	4
>>END_MODULE