FastQCFastQC Report
Thu 26 May 2016
SRR805353_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805353_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4659822
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1367802.935305254149193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC203200.43606815882666766No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG76210.1635470196071867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG59230.12710785948476144No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA47350.10161332342737556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC697350.045.65540712
GTATCAA696600.045.640479
AAGCAGT695200.045.6106831
GTGGTAT697550.045.581686
AGTGGTA696350.045.569115
ATCAACG699000.045.55436311
GGTATCA698300.045.542828
ACGCAGA698950.045.53072415
CAACGCA699700.045.50879313
TGGTATC698900.045.4667327
AACGCAG700950.045.42092514
CGCAGAG700950.045.32705316
TATCAAC701550.045.3016910
AGAGTAC688300.045.2143719
CAGAGTA695550.044.8883618
AGCAGTG707950.044.8855172
CAGTGGT707700.044.884774
GCAGTGG710450.044.780493
GCAGAGT699850.044.78045317
GTACATG470100.044.67549522