##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805353_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4659822
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.66759610131031	37.0	35.0	37.0	35.0	37.0
2	35.733894985688295	37.0	35.0	37.0	35.0	37.0
3	37.43019840672026	39.0	37.0	39.0	35.0	39.0
4	37.397062377060756	39.0	37.0	39.0	35.0	39.0
5	37.32482227861922	39.0	37.0	39.0	35.0	39.0
6	37.35484617223576	39.0	37.0	39.0	35.0	39.0
7	37.16863562599602	39.0	37.0	39.0	34.0	39.0
8	38.1658434592566	40.0	38.0	40.0	34.0	40.0
9	38.18435317915577	40.0	38.0	40.0	34.0	40.0
10	37.75899444227698	40.0	37.0	40.0	33.0	40.0
11	37.99765613364631	40.0	37.0	40.0	34.0	40.0
12	38.02094157244633	40.0	37.0	40.0	34.0	40.0
13	38.14015449517171	40.0	38.0	40.0	34.0	40.0
14	38.06170300067256	40.0	38.0	40.0	34.0	40.0
15	38.06500849174067	40.0	38.0	40.0	34.0	40.0
16	37.944021896115345	40.0	37.0	40.0	34.0	40.0
17	37.838156264338	40.0	37.0	40.0	33.0	40.0
18	37.76504231277504	40.0	37.0	40.0	33.0	40.0
19	37.741864388811415	40.0	37.0	40.0	33.0	40.0
20	37.604147755000085	40.0	37.0	40.0	33.0	40.0
21	37.338558640222736	39.0	37.0	40.0	32.0	40.0
22	37.32688501835478	39.0	37.0	40.0	32.0	40.0
23	36.784463226277744	39.0	36.0	40.0	31.0	40.0
24	36.93937214769148	39.0	36.0	40.0	31.0	40.0
25	36.98384444727717	39.0	36.0	40.0	31.0	40.0
26	36.92177984480952	39.0	36.0	40.0	31.0	40.0
27	36.8576915598922	39.0	36.0	40.0	31.0	40.0
28	36.81235377660349	39.0	36.0	40.0	31.0	40.0
29	36.65663495300893	39.0	36.0	40.0	30.0	40.0
30	36.554174601519115	39.0	36.0	40.0	30.0	40.0
31	36.36102623662449	38.0	35.0	40.0	30.0	40.0
32	36.21779887729617	38.0	35.0	40.0	30.0	40.0
33	36.138405072125074	38.0	35.0	40.0	30.0	40.0
34	35.984686754129235	38.0	35.0	40.0	30.0	40.0
35	35.944545950467635	38.0	35.0	40.0	29.0	40.0
36	35.79359833916403	38.0	35.0	40.0	29.0	40.0
37	35.55024011646797	38.0	34.0	40.0	29.0	40.0
38	35.452659135906906	38.0	34.0	40.0	28.0	40.0
39	35.40747479195557	38.0	34.0	40.0	28.0	40.0
40	35.31936284261502	38.0	34.0	40.0	28.0	40.0
41	35.1003164927759	38.0	33.0	40.0	27.0	40.0
42	34.987395441285095	37.0	33.0	40.0	26.0	40.0
43	34.83514971172718	37.0	33.0	40.0	26.0	40.0
44	34.75430370516299	37.0	33.0	40.0	26.0	40.0
45	34.520279959191576	37.0	33.0	40.0	26.0	40.0
46	34.69866402622246	37.0	33.0	40.0	26.0	40.0
47	34.640178959625494	37.0	33.0	40.0	26.0	40.0
48	34.664123007273666	37.0	33.0	40.0	26.0	40.0
49	34.589118210953124	37.0	33.0	40.0	26.0	40.0
50	34.37001306058472	37.0	33.0	40.0	26.0	40.0
51	34.088765836978325	36.0	33.0	39.0	24.0	40.0
52	33.78490122584081	36.0	32.0	39.0	23.0	40.0
53	33.521836027213055	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	12.0
15	24.0
16	74.0
17	231.0
18	559.0
19	1242.0
20	2433.0
21	4504.0
22	7326.0
23	11353.0
24	16612.0
25	23618.0
26	32224.0
27	42880.0
28	56666.0
29	73307.0
30	95786.0
31	125266.0
32	162652.0
33	213299.0
34	282926.0
35	380850.0
36	520439.0
37	732620.0
38	969425.0
39	903488.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.747880927640583	37.40741599142628	27.39941568583521	20.445287395097925
2	23.82790587279943	39.78381577665413	17.68546094679153	18.702817403754906
3	36.955231337162665	22.76529017632004	19.439691043992667	20.839787442524628
4	21.02848563743422	21.995432443556854	20.241717387488194	36.73436453152073
5	21.129154718785394	36.690564575213386	21.527775095271878	20.65250561072934
6	35.799972616979794	22.194431461115897	20.612632843057096	21.39296307884722
7	21.34248904786492	21.80922361412088	36.21653359291406	20.631753745100134
8	34.96185047411682	22.735095889928843	21.691751315822792	20.611302320131543
9	34.8820190985836	22.4114139982171	21.945859734556382	20.760707168642924
10	20.396186807135553	22.63869735796775	36.29859681335467	20.666519021542022
11	20.298457752248904	36.829625680980946	22.156039436699512	20.715877130070634
12	20.198775833068304	22.526053570286592	36.44476119474091	20.83040940190419
13	20.186844046832693	22.57322275400219	22.299263791621225	34.94066940754389
14	20.4127754236106	36.85115010830886	22.037622896325225	20.698451571755317
15	20.273521177418367	36.855892778737044	21.9925353371867	20.878050706657895
16	20.36637880159371	22.409740114536564	22.119814877048952	35.104066206820775
17	34.81907678018602	22.24157918478431	22.034961850474115	20.904382184555548
18	20.533316508656338	22.289563850292993	22.02858821645977	35.14853142459089
19	20.61110917970686	36.67150805331191	22.007428609934028	20.7099541570472
20	35.109989179844206	22.323835545649597	22.04912548161711	20.517049792889082
21	20.8209669811422	36.487874429538294	21.99408046058412	20.697078128735388
22	35.01099398217357	22.16490243618748	22.206856828436795	20.617246753202163
23	20.991574356273695	22.165632077791813	36.24149162779179	20.6013019381427
24	20.75184416915496	36.3490279242426	22.08271045546375	20.81641745113869
25	20.64302026987297	22.365553877379867	22.046163995105392	34.945261857641775
26	20.854938235838194	31.84866288883996	26.553846906598576	20.74255196872327
27	20.948611341806618	22.270679008769005	36.13560775497433	20.645101894450047
28	30.65239401848397	22.09447056132187	26.582968190630456	20.670167229563706
29	30.83008320918696	22.15554585561423	26.36218722517727	20.65218371002154
30	30.60101866551984	22.102603919205496	26.425408524188263	20.8709688910864
31	26.67893752164782	23.558131619619804	27.491329067934355	22.27160179079802
32	24.436877631806535	25.364831532191573	27.959995038437093	22.2382957975648
33	23.932716743257576	24.774358333859105	28.3845176918775	22.90840723100582
34	23.425143707205983	24.2350458880189	29.07832101741225	23.261489387362865
35	23.244578870180018	24.86150758548288	28.71854332633307	23.175370218004034
36	23.26035200486199	24.80414487935376	28.786550215866612	23.148952899917635
37	23.70056195279562	24.255196872326884	29.1251039202785	22.919137254598994
38	23.894603699454613	24.331015219036264	28.860372778187664	22.914008303321456
39	24.240646960334537	24.29663622344373	28.550103415967392	22.912613400254344
40	23.430530179049757	24.528576413433818	28.436687066587524	23.6042063409289
41	23.64545255162107	25.123277241062	28.313205955077255	22.918064252239677
42	23.76067154496459	24.97357195189001	28.437438168239044	22.828318334906356
43	23.774899556249142	24.521408757673576	28.950440596228784	22.753251089848497
44	23.74464088971639	24.39537390054813	28.430034451959752	23.429950757775725
45	23.689359808164344	24.191481992230603	28.4302705124788	23.688887687126247
46	23.513430341330636	24.260282903510046	28.464499287741035	23.761787467418284
47	23.64669723435788	24.445483110728265	28.29921400431175	23.608605650602104
48	23.95323254836773	24.35462127094125	28.34481660458275	23.347329576108272
49	23.24357024796226	25.171626727372846	28.18038972304092	23.404413301623965
50	23.81339888090146	24.6713715674118	28.375075271115506	23.14015428057123
51	23.636825612652157	24.632614722193253	28.11635294223685	23.614206722917743
52	23.886084060721632	24.612850018734623	27.7731853276799	23.727880592863848
53	24.262922489313972	24.73661011085831	27.286728978059678	23.713738421768042
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1982.0
1	1131.5
2	281.0
3	254.5
4	228.0
5	246.5
6	265.0
7	315.5
8	426.0
9	486.0
10	749.0
11	1012.0
12	1340.0
13	1668.0
14	2201.0
15	2734.0
16	3868.5
17	5003.0
18	6794.0
19	8585.0
20	12119.5
21	15654.0
22	94575.5
23	173497.0
24	116663.0
25	58589.5
26	57350.0
27	60830.0
28	64310.0
29	68113.0
30	71916.0
31	76894.5
32	81873.0
33	90521.0
34	99169.0
35	110765.5
36	122362.0
37	136087.0
38	149812.0
39	163827.0
40	177842.0
41	192876.0
42	223894.5
43	239879.0
44	259397.0
45	278915.0
46	295699.5
47	312484.0
48	333588.0
49	354692.0
50	354145.0
51	353598.0
52	349832.0
53	346066.0
54	337264.5
55	328463.0
56	312258.5
57	296054.0
58	270032.5
59	216624.0
60	189237.0
61	167161.5
62	145086.0
63	125463.0
64	105840.0
65	86700.0
66	67560.0
67	55375.5
68	43191.0
69	34332.0
70	25473.0
71	19135.5
72	12798.0
73	9383.0
74	5968.0
75	4490.0
76	2241.0
77	1470.0
78	1130.5
79	791.0
80	670.0
81	549.0
82	452.5
83	356.0
84	243.0
85	130.0
86	85.5
87	41.0
88	29.0
89	17.0
90	11.5
91	6.0
92	3.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	4659822.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.24571085134093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.11173498246424	54.879299398704795
2	9.191311430888046	11.993872959286923
3	2.853961887065676	5.5862631619270315
4	1.2831681813160336	3.348848805271478
5	0.7248840079141585	2.3647786190564157
6	0.4452562716095859	1.7430637171311096
7	0.2901826450630461	1.3253221067702559
8	0.20543070721473702	1.0722778018335366
9	0.15428139862694196	0.905957957209853
>10	0.6734708526435417	7.473944837016301
>50	0.0402317528966226	1.8187479877837383
>100	0.024791596369357995	2.73780687966624
>500	5.973631646580658E-4	0.248446366688659
>1k	5.641755704502806E-4	0.8064212799546291
>5k	6.637359652342367E-5	0.2932677232149584
>10k+	6.637359652342367E-5	3.401680398484197
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	136780	2.935305254149193	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	20320	0.43606815882666766	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	7621	0.1635470196071867	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	5923	0.12710785948476144	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	4735	0.10161332342737556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0044066060892457E-4	0.0	0.0	0.0	0.0
2	4.506609909133868E-4	0.0	0.0	0.0	2.1460047186351754E-5
3	4.721210380997386E-4	0.0	0.0	0.0	4.292009437270351E-5
4	4.721210380997386E-4	0.0	0.0	0.0	4.292009437270351E-5
5	4.721210380997386E-4	0.0	0.0	0.0	4.292009437270351E-5
6	5.150411324724421E-4	0.0	0.0	0.0	4.292009437270351E-5
7	5.150411324724421E-4	0.0	0.0	0.0	6.438014155905527E-5
8	5.150411324724421E-4	0.0	0.0	0.0	6.438014155905527E-5
9	5.365011796587938E-4	0.0	0.0	0.0	6.438014155905527E-5
10	5.365011796587938E-4	0.0	0.0	0.0	8.584018874540702E-5
11	5.365011796587938E-4	0.0	0.0	0.0	8.584018874540702E-5
12	5.579612268451456E-4	0.0	0.0	0.0	8.584018874540702E-5
13	5.579612268451456E-4	0.0	0.0	0.0	1.0730023593175877E-4
14	5.794212740314974E-4	0.0	0.0	0.0	1.2876028311811053E-4
15	5.794212740314974E-4	0.0	0.0	0.0	1.2876028311811053E-4
16	6.652614627769044E-4	0.0	0.0	0.0	1.5022033030446228E-4
17	7.081815571496078E-4	0.0	0.0	0.0	1.7168037749081403E-4
18	7.081815571496078E-4	0.0	0.0	0.0	1.7168037749081403E-4
19	8.154817930813666E-4	0.0	0.0	0.0	1.9314042467716579E-4
20	9.013219818267736E-4	0.0	0.0	0.0	2.1460047186351754E-4
21	0.0010086222177585324	0.0	0.0	0.0	2.1460047186351754E-4
22	0.0010730023593175876	0.0	0.0	0.0	2.1460047186351754E-4
23	0.0011803025952493464	0.0	0.0	0.0	2.360605190498693E-4
24	0.0014378231614855675	0.0	0.0	0.0	2.5752056623622107E-4
25	0.0015236633502309744	0.0	0.0	0.0	2.5752056623622107E-4
26	0.0019314042467716579	0.0	0.0	2.1460047186351754E-5	2.789806134225728E-4
27	0.0020172444355170648	0.0	0.0	2.1460047186351754E-5	3.0044066060892457E-4
28	0.00208162457707612	0.0	0.0	4.292009437270351E-5	3.648208021679798E-4
29	0.0022103848601942306	0.0	0.0	4.292009437270351E-5	3.8628084935433157E-4
30	0.0024679054264304516	0.0	0.0	4.292009437270351E-5	3.8628084935433157E-4
31	0.0032404671251391147	0.0	0.0	4.292009437270351E-5	4.077408965406833E-4
32	0.004013028823847778	0.0	0.0	4.292009437270351E-5	4.292009437270351E-4
33	0.004184709201338592	0.0	0.0	4.292009437270351E-5	4.292009437270351E-4
34	0.004463689814761165	0.0	0.0	4.292009437270351E-5	4.506609909133868E-4
35	0.004849970664115496	0.0	0.0	8.584018874540702E-5	4.506609909133868E-4
36	0.005300631655028883	0.0	0.0	8.584018874540702E-5	4.506609909133868E-4
37	0.006330713919973767	0.0	0.0	8.584018874540702E-5	4.506609909133868E-4
38	0.007232035901800541	0.0	0.0	8.584018874540702E-5	4.506609909133868E-4
39	0.007639776798341224	0.0	0.0	8.584018874540702E-5	4.721210380997386E-4
40	0.008176277978000019	0.0	0.0	8.584018874540702E-5	4.935810852860904E-4
41	0.009120520054199496	0.0	0.0	8.584018874540702E-5	4.935810852860904E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	69735	0.0	45.655407	12
GTATCAA	69660	0.0	45.64047	9
AAGCAGT	69520	0.0	45.610683	1
GTGGTAT	69755	0.0	45.58168	6
AGTGGTA	69635	0.0	45.56911	5
ATCAACG	69900	0.0	45.554363	11
GGTATCA	69830	0.0	45.54282	8
ACGCAGA	69895	0.0	45.530724	15
CAACGCA	69970	0.0	45.508793	13
TGGTATC	69890	0.0	45.466732	7
AACGCAG	70095	0.0	45.420925	14
CGCAGAG	70095	0.0	45.327053	16
TATCAAC	70155	0.0	45.30169	10
AGAGTAC	68830	0.0	45.21437	19
CAGAGTA	69555	0.0	44.88836	18
AGCAGTG	70795	0.0	44.885517	2
CAGTGGT	70770	0.0	44.88477	4
GCAGTGG	71045	0.0	44.78049	3
GCAGAGT	69985	0.0	44.780453	17
GTACATG	47010	0.0	44.675495	22
>>END_MODULE