FastQCFastQC Report
Thu 26 May 2016
SRR805352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10658162
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT837560.785839059304972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT572700.043.2291
TCAACGC530950.043.1537812
ACGCAGA578400.042.79892715
GTATCAA533950.042.726479
GGTATCA533250.042.7208638
GTGGTAT578100.042.5650446
CAACGCA539550.042.47900813
AGTGGTA579350.042.4164165
ATCAACG540450.042.4082711
TGGTATC536350.042.368797
AACGCAG585350.042.36303314
CGCAGAG584100.042.30482516
AGAGTAC576800.042.1109619
TATCAAC548300.041.5996710
CAGAGTA590200.041.28227618
CAGTGGT600250.041.068724
GCAGAGT597750.040.87093417
GCAGTGG607050.040.76743
AGCAGTG609200.040.584952
GAGTACA406450.040.25267820