FastQCFastQC Report
Thu 26 May 2016
SRR805351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17701541
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5682863.210375865016498No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG853200.4819919350524341No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG560540.3166616962896055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC403630.22801969613831927No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG374020.21129233889863033No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG369550.20876713501948785No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA279380.15782806705924643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTT207490.11721578364279134No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC204180.11534588994257619No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGT190670.10771378604834461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGC538750.046.07526812
AAGCAGT4078400.045.668391
AAACGCA545800.045.53178813
GTGGTAT4069500.045.514186
ATAAACG548450.045.51317611
ACGCAGA4100450.045.49387415
TCAACGC3563000.045.4665312
AGTGGTA4068750.045.4191865
CGCAGAG4102550.045.41731316
GGTATCA3542200.045.4037868
TGGTATC3534250.045.3982057
GTATCAA3546100.045.3956079
AGAGTAC4029200.045.3774219
ATCAACG3567950.045.37249411
AACGCAG4126600.045.2494314
CAGTGGT4099750.045.161154
CAGAGTA4058600.045.15756618
AGCAGTG4125250.045.144042
GCAGTGG4128550.045.119913
CAACGCA3593500.045.09763313