##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR805351_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17701541 Sequences flagged as poor quality 0 Sequence length 53 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.673334541891016 35.0 33.0 35.0 32.0 35.0 2 34.469447659952316 35.0 35.0 35.0 32.0 37.0 3 36.68589774189716 38.0 35.0 39.0 33.0 39.0 4 36.69619102653266 38.0 35.0 39.0 33.0 39.0 5 36.740711331290306 38.0 35.0 39.0 33.0 39.0 6 36.822203671420475 39.0 37.0 39.0 33.0 39.0 7 36.46403722704142 38.0 35.0 39.0 32.0 39.0 8 37.40854799025689 39.0 37.0 40.0 32.0 40.0 9 37.38141786638802 39.0 36.0 40.0 32.0 40.0 10 36.720952938504055 38.0 36.0 40.0 32.0 40.0 11 36.987825975150976 38.0 36.0 40.0 32.0 40.0 12 37.11089774613408 39.0 36.0 40.0 32.0 40.0 13 32.5989355954942 38.0 33.0 40.0 3.0 40.0 14 34.496864256055446 38.0 33.0 40.0 18.0 40.0 15 35.941522944245364 38.0 33.0 40.0 27.0 40.0 16 36.38999740192111 38.0 35.0 40.0 31.0 40.0 17 36.55133177388341 38.0 36.0 40.0 31.0 40.0 18 36.55072001923448 38.0 36.0 40.0 31.0 40.0 19 36.50159971948205 38.0 36.0 40.0 30.0 40.0 20 36.42281341494506 38.0 36.0 40.0 30.0 40.0 21 36.10733427106713 38.0 35.0 40.0 30.0 40.0 22 36.20142127739048 38.0 35.0 40.0 30.0 40.0 23 35.620653817653505 38.0 34.0 40.0 28.0 40.0 24 35.71799946682608 38.0 34.0 40.0 29.0 40.0 25 35.78508187507517 38.0 34.0 40.0 29.0 40.0 26 35.68974040169723 38.0 34.0 40.0 29.0 40.0 27 35.70309618806634 38.0 34.0 40.0 29.0 40.0 28 35.393061654914675 38.0 34.0 40.0 27.0 40.0 29 35.133074685418634 38.0 34.0 40.0 26.0 40.0 30 30.584693445615837 37.0 29.0 39.0 3.0 40.0 31 31.921923972607807 36.0 28.0 39.0 15.0 40.0 32 33.0634172471199 36.0 30.0 39.0 22.0 40.0 33 33.543055488784844 36.0 31.0 39.0 23.0 40.0 34 33.69814373788135 37.0 31.0 39.0 23.0 40.0 35 33.748546694324524 37.0 32.0 39.0 24.0 40.0 36 33.73620629977921 36.0 32.0 39.0 24.0 40.0 37 33.45670899499654 36.0 31.0 38.0 24.0 40.0 38 33.35063687393092 36.0 31.0 38.0 24.0 40.0 39 33.207570629020374 35.0 31.0 38.0 23.0 40.0 40 33.055996311281596 35.0 31.0 38.0 23.0 40.0 41 32.86866759227346 35.0 31.0 38.0 23.0 40.0 42 32.57997792395589 35.0 30.0 38.0 22.0 40.0 43 32.477239975886846 35.0 30.0 38.0 22.0 40.0 44 32.18277165812852 35.0 30.0 38.0 21.0 40.0 45 31.905952764225443 34.0 29.0 38.0 20.0 39.0 46 31.66463925372373 34.0 29.0 38.0 20.0 39.0 47 31.418635699569887 34.0 28.0 37.0 19.0 39.0 48 31.36318007567816 34.0 28.0 37.0 20.0 39.0 49 31.06877525521648 34.0 28.0 37.0 19.0 39.0 50 30.64748131250268 33.0 28.0 37.0 18.0 39.0 51 30.478913954440465 33.0 27.0 37.0 17.0 39.0 52 30.03576762045745 33.0 26.0 36.0 14.0 39.0 53 30.992696907009396 34.0 28.0 37.0 18.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 5.0 12 26.0 13 80.0 14 234.0 15 776.0 16 2007.0 17 4978.0 18 11144.0 19 21584.0 20 38095.0 21 60927.0 22 90730.0 23 129617.0 24 177191.0 25 237124.0 26 303883.0 27 383883.0 28 476020.0 29 586575.0 30 719094.0 31 883460.0 32 1095503.0 33 1368065.0 34 1711386.0 35 2063725.0 36 2240317.0 37 2552634.0 38 2218797.0 39 323679.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.024167839398842 46.74240508213381 30.4898991562373 10.743527922230047 2 23.787109834109923 50.70887331221615 12.226144605150479 13.277872248523447 3 46.22242210438063 21.57499169140133 17.148264097459084 15.054322106758955 4 20.34359607448866 20.47969157035537 16.9943113992166 42.18240095593938 5 17.74552848252025 44.47573801625519 19.516877089966346 18.261856411258208 6 41.42470421078029 20.929319091484746 18.060518008008454 19.585458689726504 7 19.33120963875405 19.903747363012066 42.45850686106933 18.306536137164557 8 40.367536363077086 21.401854222748177 20.030064049225995 18.200545364948738 9 41.25141421303377 20.680086552916492 19.819003328580262 18.249495905469473 10 18.661640814209342 21.212277507365037 42.33584522387062 17.790236454555004 11 17.884019250075458 43.599074227492395 20.706841285738907 17.810065236693234 12 17.91708981720857 20.999318646890686 43.16055873327638 17.92303280262436 13 15.480816048727059 31.337486380423034 18.194896139268327 34.986801431581576 14 17.930727048001074 43.57016713968575 20.386552786562483 18.112553025750696 15 17.89093390230828 43.5880017451588 20.460817507357127 18.060246845175794 16 17.93966977225316 20.950836992101422 20.6181766886849 40.49131654696051 17 40.52895733766908 20.61206987572438 20.549239187706878 18.309733598899665 18 18.356927230233797 20.443915024121345 20.582112031941175 40.61704571370368 19 18.065941264661646 43.2736731790752 20.452236333548587 18.208149222714564 20 40.80791610176764 20.940476312203554 20.41682133775811 17.8347862482707 21 18.60124494246009 43.02499426462363 20.35164057185756 18.02212022105872 22 40.76120265461634 20.837383592761782 20.494582929248928 17.90683082337295 23 18.681757706857276 20.5816431462097 42.705140755824594 18.031458391108433 24 18.366610003050017 42.82435071613257 20.57860386279364 18.230435418023774 25 18.12689640975325 21.045258150123765 20.320439898424663 40.50740554169832 26 18.364892638443173 35.94793244271784 27.30201285865451 18.385162060184477 27 18.52897439833063 20.64681261365889 42.632299639901404 18.191913348109072 28 33.793634124848225 20.507242843998725 27.661591722438178 18.037531308714875 29 33.98177593690854 20.607143750931062 27.335309394814832 18.075770917345558 30 29.37023392483174 30.989708749085743 23.815604528441902 15.824452797640612 31 28.33287226236405 22.72744502865598 28.840596420390746 20.099086288589223 32 25.0518076364086 24.67342814956054 29.70171354008106 20.5730506739498 33 24.509798327727513 23.683011552497042 30.451405332451 21.355784787324446 34 22.715547759373038 24.026235907935924 30.911065878388776 22.347150454302255 35 21.947648512635144 24.270355897263407 31.543050404481733 22.238945185619716 36 22.38447488837271 23.8930836586487 32.09188397778476 21.630557475193825 37 23.337725229684807 23.48727153189657 31.734694736463904 21.440308501954718 38 23.26414406519749 23.71021257414821 31.257894439811768 21.767748920842543 39 23.818768094822932 23.659708496565354 31.019598802160786 21.50192460645093 40 22.534439233284832 24.63577041117494 30.982890133689487 21.846900221850742 41 23.34107522051329 24.99473915858512 30.437446095794712 21.226739525106883 42 23.426830466341887 25.015500062960623 30.359074387930406 21.19859508276709 43 22.744974575942287 24.810489663018604 31.529317136852665 20.915218624186448 44 23.53069148047619 24.091478815318958 30.888192163608807 21.489637540596043 45 23.480876608426353 23.666939505436275 30.95672856956352 21.89545531657385 46 22.691267387398646 23.967681683758492 30.505236803959612 22.835814124883253 47 22.453282457160086 23.987425727511518 30.8471900836204 22.71210173170799 48 22.49487205661925 23.777556993484353 30.939159477697448 22.78841147219895 49 21.96169813690232 25.48152163701454 30.259930477239244 22.2968497488439 50 23.115778451152924 24.85293229555551 30.181445784861328 21.849843468430237 51 22.910078845677898 24.331096371779157 30.661053746676632 22.097771035866312 52 23.543859825537222 23.99447596116067 30.374976958220756 22.08668725508135 53 24.62056834486896 24.333130093024106 28.872904342056998 22.173397220049935 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4987.0 1 3103.0 2 1219.0 3 1016.5 4 814.0 5 882.5 6 951.0 7 1077.0 8 1547.5 9 1892.0 10 2523.0 11 3154.0 12 4482.0 13 5810.0 14 8243.5 15 10677.0 16 15600.0 17 20523.0 18 28985.0 19 37447.0 20 99346.5 21 161246.0 22 465568.5 23 769891.0 24 562952.5 25 329679.0 26 303344.0 27 307082.5 28 310821.0 29 323143.0 30 335465.0 31 353333.0 32 371201.0 33 409105.5 34 447010.0 35 497138.0 36 547266.0 37 597517.5 38 647769.0 39 699938.5 40 752108.0 41 810521.5 42 918522.5 43 968110.0 44 1046821.5 45 1125533.0 46 1167141.0 47 1208749.0 48 1252385.0 49 1296021.0 50 1290470.0 51 1284919.0 52 1266446.5 53 1247974.0 54 1195664.5 55 1143355.0 56 1125837.5 57 1108320.0 58 951287.5 59 680914.5 60 567574.0 61 477500.5 62 387427.0 63 324615.0 64 261803.0 65 211939.5 66 162076.0 67 126674.5 68 91273.0 69 70891.5 70 50510.0 71 37624.0 72 24738.0 73 17756.5 74 10775.0 75 7742.5 76 3421.0 77 2132.0 78 1514.5 79 897.0 80 665.5 81 434.0 82 285.5 83 137.0 84 90.5 85 44.0 86 29.0 87 14.0 88 11.0 89 8.0 90 5.5 91 3.0 92 3.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 53 1.7701541E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.79015887953755 #Duplication Level Percentage of deduplicated Percentage of total 1 80.77510879304148 40.21805500317466 2 10.504011656681481 10.459928185173707 3 3.1809405794142145 4.75138610506206 4 1.4238687729334567 2.8357860971187594 5 0.8082682557606009 2.0121902435803505 6 0.5308711548510281 1.5859295486757765 7 0.37574802502754684 1.3095987705355805 8 0.28668649370321936 1.1419332856080666 9 0.22634339193383976 1.0142706101147387 >10 1.6999675472635096 16.233042842564867 >50 0.11381353164187258 3.928379134063479 >100 0.06992604650031545 6.191926863327085 >500 0.002824705414374976 0.9489760917621884 >1k 0.0013039450145706849 1.279662737660317 >5k 1.761671212687244E-4 0.5913962814657422 >10k+ 1.409336970149795E-4 5.497538200112309 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 568286 3.210375865016498 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG 85320 0.4819919350524341 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG 56054 0.3166616962896055 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC 40363 0.22801969613831927 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG 37402 0.21129233889863033 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG 36955 0.20876713501948785 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA 27938 0.15782806705924643 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTT 20749 0.11721578364279134 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC 20418 0.11534588994257619 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGT 19067 0.10771378604834461 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2428296496898208E-4 0.0 0.0 5.649225680408277E-6 1.1298451360816553E-5 2 0.0014857463539473766 0.0 0.0 7.343993384530759E-5 1.694767704122483E-5 3 0.0014857463539473766 0.0 0.0 7.343993384530759E-5 3.389535408244966E-5 4 0.0015987308675555422 0.0 0.0 9.038761088653243E-5 5.084303112367449E-5 5 0.0015987308675555422 0.0 0.0 1.0168606224734897E-4 6.779070816489932E-5 6 0.0016100293189163588 0.0 0.0 1.0168606224734897E-4 7.343993384530759E-5 7 0.001615678544596767 0.0 0.0 1.0168606224734897E-4 9.038761088653243E-5 8 0.001615678544596767 0.0 0.0 1.0733528792775725E-4 1.2428296496898208E-4 9 0.001615678544596767 0.0 0.0 1.0733528792775725E-4 1.412306420102069E-4 10 0.0016213277702771753 0.0 0.0 1.186337392885738E-4 1.4687986769061517E-4 11 0.0016269769959575836 0.0 0.0 1.186337392885738E-4 1.6382754473184E-4 12 0.001632626221637992 0.0 0.0 1.186337392885738E-4 1.920736731338814E-4 13 0.0016439246729988083 0.0 0.0 1.186337392885738E-4 2.0902135017510621E-4 14 0.0016721708014008497 0.0 0.0 1.186337392885738E-4 2.2596902721633104E-4 15 0.0016891184784420747 0.0 0.0 1.2428296496898208E-4 2.4856592993796416E-4 16 0.001700416929802891 0.0 0.0 1.2428296496898208E-4 2.598643812987807E-4 17 0.0017060661554832994 0.0 0.0 1.2993219064939035E-4 2.881105097008221E-4 18 0.0017060661554832994 0.0 0.0 1.3558141632979864E-4 3.107074124224552E-4 19 0.0017286630582049325 0.0 0.0 2.0337212449469795E-4 3.2200586378327173E-4 20 0.0017456107352461574 0.0 0.0 2.146705758555145E-4 3.784981205873545E-4 21 0.0017456107352461574 0.0 1.1298451360816553E-5 2.9375973538123035E-4 3.8979657194817104E-4 22 0.0017851553150090153 0.0 1.1298451360816553E-5 2.9375973538123035E-4 4.0109502330898763E-4 23 0.0018303491204522816 0.0 1.1298451360816553E-5 2.9375973538123035E-4 4.2369192603062075E-4 24 0.001858595248854323 0.0 1.1298451360816553E-5 2.9375973538123035E-4 4.29341151711029E-4 25 0.0018755429258955477 0.0 1.1298451360816553E-5 2.9375973538123035E-4 4.4063960307184555E-4 26 0.0018924906029367725 0.0 1.1298451360816553E-5 2.9375973538123035E-4 4.519380544326621E-4 27 0.0019489828597408553 3.389535408244966E-5 1.1298451360816553E-5 2.9940896106163867E-4 4.745349571542952E-4 28 0.0019941766651841216 3.389535408244966E-5 1.1298451360816553E-5 3.0505818674204693E-4 4.971318598759283E-4 29 0.002107161178792287 3.389535408244966E-5 1.1298451360816553E-5 3.0505818674204693E-4 5.084303112367449E-4 30 0.0021580042099159616 3.9544579762857933E-5 1.1298451360816553E-5 3.0505818674204693E-4 5.197287625975614E-4 31 0.0022596902721633105 3.9544579762857933E-5 1.1298451360816553E-5 3.0505818674204693E-4 5.366764396387862E-4 32 0.0023331302060086183 3.9544579762857933E-5 1.1298451360816553E-5 3.0505818674204693E-4 5.423256653191946E-4 33 0.002372674785771476 3.9544579762857933E-5 1.1298451360816553E-5 3.0505818674204693E-4 5.536241166800111E-4 34 0.002417868591214742 3.9544579762857933E-5 1.1298451360816553E-5 3.107074124224552E-4 5.536241166800111E-4 35 0.00249130852506005 3.9544579762857933E-5 1.1298451360816553E-5 3.2765508946368E-4 5.762210194016442E-4 36 0.002576046910266174 3.9544579762857933E-5 1.1298451360816553E-5 3.333043151440883E-4 5.762210194016442E-4 37 0.002672083746833115 3.9544579762857933E-5 1.1298451360816553E-5 3.333043151440883E-4 6.044671478036855E-4 38 0.002756822132039239 3.9544579762857933E-5 1.1298451360816553E-5 3.333043151440883E-4 6.157655991645021E-4 39 0.00281896361452373 3.9544579762857933E-5 1.1298451360816553E-5 3.389535408244966E-4 6.214148248449104E-4 40 0.002937597353812304 3.9544579762857933E-5 1.1298451360816553E-5 3.389535408244966E-4 6.440117275665435E-4 41 0.003044932641740061 3.9544579762857933E-5 1.1298451360816553E-5 3.4460276650490485E-4 6.609594046077683E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAACGC 53875 0.0 46.075268 12 AAGCAGT 407840 0.0 45.66839 1 AAACGCA 54580 0.0 45.531788 13 GTGGTAT 406950 0.0 45.51418 6 ATAAACG 54845 0.0 45.513176 11 ACGCAGA 410045 0.0 45.493874 15 TCAACGC 356300 0.0 45.46653 12 AGTGGTA 406875 0.0 45.419186 5 CGCAGAG 410255 0.0 45.417313 16 GGTATCA 354220 0.0 45.403786 8 TGGTATC 353425 0.0 45.398205 7 GTATCAA 354610 0.0 45.395607 9 AGAGTAC 402920 0.0 45.37742 19 ATCAACG 356795 0.0 45.372494 11 AACGCAG 412660 0.0 45.24943 14 CAGTGGT 409975 0.0 45.16115 4 CAGAGTA 405860 0.0 45.157566 18 AGCAGTG 412525 0.0 45.14404 2 GCAGTGG 412855 0.0 45.11991 3 CAACGCA 359350 0.0 45.097633 13 >>END_MODULE