FastQCFastQC Report
Thu 26 May 2016
SRR805350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16705103
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510833.298890165478178No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG382980.22925928681792623No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG367940.2202560499028351No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG281060.16824798985076594No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTT247030.14787696909142076No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA237750.14232178035657728No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG234550.14040619803421744No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC186550.11167246319882015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2062050.045.5104871
TCAACGC2062750.045.4950412
GGTATCA2054450.045.4237638
GTATCAA2058150.045.39129
TGGTATC2055550.045.3811657
ACGCAGA2063550.045.30544315
ATCAACG2075350.045.17693311
CGCAGAG2069100.045.14303216
AGAGTAC2027500.045.0805919
GTGGTAT2078650.044.8971946
AGTGGTA2076000.044.8582885
CAGAGTA2051450.044.69748318
GCAGAGT2064750.044.58370217
TATCAAC2094250.044.5672410
CAGTGGT2098500.044.4669044
CAACGCA2111800.044.38270613
GAGTACT897050.044.36982320
AACGCAG2119400.044.19694514
GAGTACA1169950.044.17988220
GCAGTGG2123100.044.176373