##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805350_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16705103
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.93838433681014	35.0	33.0	35.0	32.0	36.0
2	34.851999475848785	35.0	35.0	37.0	33.0	37.0
3	37.158478639730625	39.0	37.0	39.0	34.0	39.0
4	37.255253140312874	39.0	37.0	39.0	34.0	39.0
5	37.263427169530175	39.0	37.0	39.0	34.0	39.0
6	37.3183858848401	39.0	37.0	39.0	34.0	39.0
7	37.115224371858105	39.0	37.0	39.0	33.0	39.0
8	38.04188827809083	40.0	38.0	40.0	34.0	40.0
9	38.04311604663557	40.0	38.0	40.0	34.0	40.0
10	37.67975444389658	40.0	37.0	40.0	33.0	40.0
11	37.82279127521692	40.0	37.0	40.0	33.0	40.0
12	37.886955141791105	40.0	38.0	40.0	33.0	40.0
13	37.988367985519155	40.0	38.0	40.0	34.0	40.0
14	37.900436651004185	40.0	38.0	40.0	34.0	40.0
15	37.8596759924198	40.0	38.0	40.0	33.0	40.0
16	37.73425084538539	40.0	37.0	40.0	33.0	40.0
17	37.65329755823714	40.0	37.0	40.0	33.0	40.0
18	37.57667707885429	40.0	37.0	40.0	33.0	40.0
19	37.4310381683968	40.0	37.0	40.0	32.0	40.0
20	37.38496338514046	40.0	37.0	40.0	32.0	40.0
21	37.14303306001765	39.0	37.0	40.0	32.0	40.0
22	37.21228172014264	39.0	37.0	40.0	32.0	40.0
23	36.671063806071714	39.0	36.0	40.0	30.0	40.0
24	36.873051965019314	39.0	36.0	40.0	31.0	40.0
25	36.879179314249065	39.0	36.0	40.0	31.0	40.0
26	36.806118166406996	39.0	36.0	40.0	30.0	40.0
27	36.8228103711782	39.0	36.0	40.0	30.0	40.0
28	36.671107146121756	39.0	36.0	40.0	30.0	40.0
29	36.60506696666282	39.0	36.0	40.0	30.0	40.0
30	36.52419557065886	39.0	36.0	40.0	30.0	40.0
31	36.361982203881055	39.0	35.0	40.0	30.0	40.0
32	36.24557322394241	39.0	35.0	40.0	30.0	40.0
33	36.066507880855326	38.0	35.0	40.0	30.0	40.0
34	35.91214193650886	38.0	35.0	40.0	29.0	40.0
35	35.712122098259435	38.0	35.0	40.0	28.0	40.0
36	35.679007546376695	38.0	35.0	40.0	28.0	40.0
37	35.4452794454485	38.0	34.0	40.0	28.0	40.0
38	35.54548235949218	38.0	34.0	40.0	28.0	40.0
39	35.617308854665545	38.0	34.0	40.0	28.0	40.0
40	35.57367769597111	38.0	34.0	40.0	28.0	40.0
41	35.54473396542362	38.0	34.0	40.0	28.0	40.0
42	35.46457965568964	38.0	34.0	40.0	28.0	40.0
43	35.41623861882204	38.0	34.0	40.0	28.0	40.0
44	35.28411863129488	38.0	34.0	40.0	27.0	40.0
45	35.16829348493092	38.0	34.0	40.0	27.0	40.0
46	35.02575859604098	37.0	34.0	40.0	27.0	40.0
47	34.90184089257037	37.0	33.0	40.0	27.0	40.0
48	34.81126312121511	37.0	33.0	40.0	26.0	40.0
49	34.5721259545661	37.0	33.0	40.0	26.0	40.0
50	34.36947955364298	37.0	33.0	40.0	26.0	40.0
51	34.068240944099536	36.0	33.0	39.0	24.0	40.0
52	33.729236389622976	36.0	32.0	39.0	23.0	40.0
53	33.434163440955736	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	9.0
11	19.0
12	64.0
13	148.0
14	344.0
15	784.0
16	1698.0
17	3710.0
18	7197.0
19	13278.0
20	21643.0
21	33722.0
22	48167.0
23	66143.0
24	88329.0
25	115074.0
26	145986.0
27	182372.0
28	223986.0
29	275441.0
30	337706.0
31	416801.0
32	520459.0
33	662667.0
34	879281.0
35	1210662.0
36	1754876.0
37	2649076.0
38	3661141.0
39	3384319.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.579042284264876	44.512296631753784	21.764128003281392	20.14453308069995
2	24.509247264144378	42.36805364205177	17.74309323324735	15.379605860556502
3	35.28232660403231	23.710323725630424	21.091884318223002	19.91546535211426
4	20.466368869440675	23.789796447229328	21.359706671667933	34.384128011662064
5	19.45692283369938	36.30287104485378	23.8699755398096	20.370230581637237
6	32.437680869133224	22.854579226479476	22.712658521171644	21.995081383215656
7	21.81716568883173	23.16838752805056	34.55066394981222	20.463782833305487
8	32.01557033201172	23.680937495566475	23.561237545198015	20.742254627223787
9	32.54639016592714	23.073033431760344	23.544685716693873	20.83589068561864
10	20.740626382249783	23.955925324135986	34.86219151118075	20.441256782433488
11	20.401514435439278	35.635919155960906	23.345872216411955	20.61669419218786
12	20.49879608644137	23.388619633174365	35.23169536877444	20.880888911609823
13	20.547218415833772	23.893525229985112	23.219174404372126	32.34008194980899
14	20.700315346753623	35.68653243263451	23.076224073566024	20.536928147045845
15	20.59564673142093	35.553644895215555	22.947059949286157	20.90364842407736
16	20.738938275328202	23.60983946043314	23.147310136309844	32.503912127928814
17	32.68781401707012	23.34659055978284	23.193266153462208	20.77232926968484
18	20.7192496807712	23.200419656197273	23.113380384425046	32.96695027860648
19	21.0210197446852	35.36079364491197	22.93298041921681	20.685206191186012
20	32.932960664774114	23.469265648945715	22.96875391908688	20.629019767193295
21	21.154098840336395	35.16230339914696	22.86601884466082	20.81757891585583
22	32.81787008436883	23.389517562387972	23.053877608536745	20.738734744706452
23	20.97298651795203	23.34780575731859	34.97908992240275	20.70011780232663
24	21.05980430051823	34.99677912791079	22.92162460776207	21.0217919638089
25	20.836315705446413	23.514814604854575	22.92397718230172	32.72489250739729
26	20.89599208098268	29.945544184911636	28.27837098639859	20.880092747707092
27	21.17644530536567	23.10232986890293	34.728519782248576	20.99270504348282
28	27.91730167721803	23.241257476832082	28.0741040626927	20.767336783257186
29	27.873566538320656	23.3721276666178	27.98110852713689	20.773197267924655
30	27.791831035103463	23.127855003348376	28.07922225921026	21.001091702337902
31	25.55496964011536	24.164077288239408	28.55994961539597	21.721003456249267
32	24.15365532316682	24.985161719745157	29.032062837325817	21.82912011976221
33	23.95745778999387	24.471929326026903	29.240963075773912	22.329649808205314
34	22.893190182664544	25.055272032743524	29.353635233497215	22.697902551094717
35	22.779105282978502	25.07520606128558	29.770819132333393	22.37486952340252
36	22.907275698928643	24.61580153082564	30.17262449683788	22.304298273407834
37	23.11724746623831	24.58962390115164	30.0094348415571	22.28369379105295
38	23.01660755997733	24.626343219793377	29.771298027913986	22.585751192315307
39	23.614592499070493	24.442076172771877	29.538991767964557	22.404339560193073
40	22.909831804090043	25.02465264655956	29.66349264652843	22.40202290282197
41	23.217576090371907	25.108172035814448	29.50126078240882	22.17299109140482
42	23.391409199931303	24.99139933468234	29.328936193928286	22.28825527145807
43	22.936141130048703	25.32879324359748	29.708293328092623	22.026772298261196
44	23.245848888210986	24.932501164464536	29.492185711156644	22.329464236167833
45	23.298407678180734	24.636447916543823	29.609521114595942	22.4556232906795
46	22.739380894568566	25.09188958607439	29.333288157516897	22.83544136184015
47	22.82782692210877	24.93344099704144	29.388456928400856	22.850275152448926
48	23.08548471685568	24.476778143780376	29.483248322383886	22.954488816980056
49	22.754178768008792	25.523153014979915	29.081568667969304	22.64109954904199
50	23.087945042900962	25.341908996310885	29.143004984764236	22.427140976023914
51	23.153835088595383	25.001264583642495	29.176186462304365	22.66871386545776
52	23.452977213010897	24.988430182082684	28.950746367741644	22.607846237164775
53	23.963677446346786	25.105735654548194	28.204028433706753	22.726558465398266
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15705.0
1	9095.5
2	2486.0
3	2119.5
4	1753.0
5	1833.5
6	1914.0
7	2100.5
8	2819.5
9	3352.0
10	4381.0
11	5410.0
12	7145.0
13	8880.0
14	11525.5
15	14171.0
16	20018.0
17	25865.0
18	35206.5
19	44548.0
20	61151.5
21	77755.0
22	401831.0
23	725907.0
24	493235.0
25	259626.5
26	258690.0
27	277115.5
28	295541.0
29	310298.5
30	325056.0
31	338527.0
32	351998.0
33	390007.5
34	428017.0
35	472635.5
36	517254.0
37	563444.0
38	609634.0
39	655901.5
40	702169.0
41	740348.5
42	820929.0
43	863330.0
44	937673.0
45	1012016.0
46	1050243.5
47	1088471.0
48	1145061.0
49	1201651.0
50	1212297.0
51	1222943.0
52	1195293.5
53	1167644.0
54	1110826.0
55	1054008.0
56	1012887.5
57	971767.0
58	882894.5
59	716416.0
60	638810.0
61	544825.5
62	450841.0
63	382913.5
64	314986.0
65	260053.0
66	205120.0
67	162461.0
68	119802.0
69	93927.0
70	68052.0
71	53571.5
72	39091.0
73	27993.5
74	16896.0
75	12698.5
76	6439.5
77	4378.0
78	3389.5
79	2401.0
80	1970.0
81	1539.0
82	1172.0
83	805.0
84	545.5
85	286.0
86	208.5
87	131.0
88	96.5
89	62.0
90	46.5
91	31.0
92	27.0
93	14.5
94	6.0
95	5.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.6705103E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.2040546413586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.40778133825685	29.422901240797227
2	15.157981391602283	12.491405870245547
3	5.3164091363235375	6.571728376466796
4	2.4319975115713817	4.008326334177415
5	1.3124131129822814	2.703837080967873
6	0.8254437164430558	2.040697679741147
7	0.56835012867961	1.639283083027649
8	0.38708899686718923	1.2759708942387484
9	0.3182531940399511	1.1801989797308141
>10	2.020133022758614	15.492074315772578
>50	0.145862412846467	4.174750194997098
>100	0.09062368755951224	7.474066802301
>500	0.013095309719341227	3.7805346596628273
>1k	0.0042898548600181765	2.7014009668673764
>5k	1.0212096993396155E-4	0.23894281643028967
>10k+	1.7506451988679123E-4	4.803880704575587
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	551083	3.298890165478178	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG	38298	0.22925928681792623	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	36794	0.2202560499028351	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	28106	0.16824798985076594	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTT	24703	0.14787696909142076	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	23775	0.14232178035657728	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	23455	0.14040619803421744	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	18655	0.11167246319882015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1972389514748877E-5	0.0	0.0	0.0	1.7958584272123314E-5
2	2.3944779029497754E-5	0.0	0.0	0.0	1.7958584272123314E-5
3	2.3944779029497754E-5	0.0	0.0	0.0	3.591716854424663E-5
4	2.993097378687219E-5	0.0	0.0	0.0	4.190336330162107E-5
5	5.986194757374438E-5	0.0	0.0	5.9861947573744384E-6	5.387575281636995E-5
6	5.986194757374438E-5	0.0	0.0	5.9861947573744384E-6	8.979292136061657E-5
7	6.584814233111883E-5	0.0	0.0	5.9861947573744384E-6	9.577911611799101E-5
8	8.380672660324214E-5	0.0	0.0	5.9861947573744384E-6	9.577911611799101E-5
9	1.0176531087536545E-4	0.0	0.0	5.9861947573744384E-6	1.3768247941961208E-4
10	1.077515056327399E-4	0.0	0.0	5.9861947573744384E-6	1.4965486893436095E-4
11	1.1373770039011433E-4	0.0	0.0	5.9861947573744384E-6	1.6162725844910984E-4
12	1.4965486893436095E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	1.6162725844910984E-4
13	1.6761345320648427E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	2.035306217507309E-4
14	1.6761345320648427E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	2.155030112654798E-4
15	1.735996479638587E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	2.2148920602285422E-4
16	1.7958584272123314E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	2.3944779029497752E-4
17	1.855720374786076E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	2.6937876408184973E-4
18	2.0951681650810533E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	2.9332354311134746E-4
19	2.0951681650810533E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	3.1726832214084525E-4
20	2.2747540078022865E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	3.3522690641296855E-4
21	2.3944779029497752E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	3.471992959277174E-4
22	2.514201798097264E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	3.7713026971458963E-4
23	2.7536495883922417E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	4.0107504874408736E-4
24	2.8733734835397303E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	4.070612435014618E-4
25	2.9332354311134746E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	4.1903363301621065E-4
26	3.1726832214084525E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	4.310060225309596E-4
27	3.41213101170343E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	4.36992217288334E-4
28	3.711440749572152E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	4.549508015604573E-4
29	4.0107504874408736E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	4.6093699631783174E-4
30	4.36992217288334E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	4.908679701047039E-4
31	4.6692319107520617E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	4.908679701047039E-4
32	5.088265543768272E-4	5.9861947573744384E-6	0.0	5.9861947573744384E-6	5.148127491342017E-4
33	5.387575281636995E-4	5.9861947573744384E-6	0.0	1.1972389514748877E-5	5.148127491342017E-4
34	5.926332809800694E-4	5.9861947573744384E-6	0.0	1.1972389514748877E-5	5.32771333406325E-4
35	6.82426202340686E-4	5.9861947573744384E-6	0.0	1.7958584272123314E-5	5.32771333406325E-4
36	7.303157603996814E-4	5.9861947573744384E-6	0.0	1.7958584272123314E-5	5.507299176784483E-4
37	7.722191237013026E-4	5.9861947573744384E-6	0.0	1.7958584272123314E-5	5.567161124358228E-4
38	8.20108681760298E-4	5.9861947573744384E-6	0.0	1.7958584272123314E-5	5.627023071931972E-4
39	8.799706293340424E-4	5.9861947573744384E-6	0.0	1.7958584272123314E-5	5.866470862226949E-4
40	9.577911611799101E-4	5.9861947573744384E-6	0.0	1.7958584272123314E-5	5.986194757374438E-4
41	0.001035611693025778	5.9861947573744384E-6	0.0	1.7958584272123314E-5	6.285504495243161E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	206205	0.0	45.510487	1
TCAACGC	206275	0.0	45.49504	12
GGTATCA	205445	0.0	45.423763	8
GTATCAA	205815	0.0	45.3912	9
TGGTATC	205555	0.0	45.381165	7
ACGCAGA	206355	0.0	45.305443	15
ATCAACG	207535	0.0	45.176933	11
CGCAGAG	206910	0.0	45.143032	16
AGAGTAC	202750	0.0	45.08059	19
GTGGTAT	207865	0.0	44.897194	6
AGTGGTA	207600	0.0	44.858288	5
CAGAGTA	205145	0.0	44.697483	18
GCAGAGT	206475	0.0	44.583702	17
TATCAAC	209425	0.0	44.56724	10
CAGTGGT	209850	0.0	44.466904	4
CAACGCA	211180	0.0	44.382706	13
GAGTACT	89705	0.0	44.369823	20
AACGCAG	211940	0.0	44.196945	14
GAGTACA	116995	0.0	44.179882	20
GCAGTGG	212310	0.0	44.17637	3
>>END_MODULE