FastQCFastQC Report
Thu 26 May 2016
SRR805349_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805349_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19432248
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820760.9369785729371095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT798700.042.90141
TCAACGC812050.042.3755312
GGTATCA804200.042.37138
GTATCAA809950.042.2881059
TGGTATC807750.042.1181687
ACGCAGA815950.042.06354515
ATCAACG821750.041.8410111
CGCAGAG824750.041.58623516
AGAGTAC813600.041.3416319
CAGAGTA836900.040.31700518
GTGGTAT845900.040.299216
GCAGAGT843000.040.14513417
AGTGGTA851550.039.962835
TATCAAC859100.039.9097910
CAACGCA865800.039.7610913
AACGCAG871950.039.4779514
GTACATG519250.039.4148322
CAGTGGT869200.039.2621964
GAGTACT331950.039.12772820
GAGTACA530700.038.98511520
Produced by FastQC (version 0.11.5)