##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805349_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19432248
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.62625826924399	37.0	35.0	37.0	35.0	37.0
2	35.65074359899071	37.0	35.0	37.0	35.0	37.0
3	37.30706586288936	39.0	37.0	39.0	34.0	39.0
4	37.34866532168589	39.0	37.0	39.0	34.0	39.0
5	37.32798855798876	39.0	37.0	39.0	34.0	39.0
6	37.297536188298956	39.0	37.0	39.0	34.0	39.0
7	37.19612054148342	39.0	37.0	39.0	34.0	39.0
8	38.045213811598124	40.0	38.0	40.0	34.0	40.0
9	38.0149218968387	40.0	38.0	40.0	34.0	40.0
10	37.8868797886894	40.0	38.0	40.0	33.0	40.0
11	37.91745818600092	40.0	38.0	40.0	33.0	40.0
12	37.852340861438165	40.0	38.0	40.0	33.0	40.0
13	37.959926870015245	40.0	38.0	40.0	34.0	40.0
14	37.84632411031395	40.0	38.0	40.0	33.0	40.0
15	37.80552188300602	40.0	38.0	40.0	33.0	40.0
16	37.70278842674301	40.0	37.0	40.0	33.0	40.0
17	37.606709012770935	40.0	37.0	40.0	33.0	40.0
18	37.555805123524564	40.0	37.0	40.0	33.0	40.0
19	37.44465704636952	40.0	37.0	40.0	32.0	40.0
20	37.31020785654856	40.0	37.0	40.0	32.0	40.0
21	37.23361172623981	40.0	37.0	40.0	32.0	40.0
22	37.16090598473218	39.0	37.0	40.0	32.0	40.0
23	36.94975789728497	39.0	36.0	40.0	31.0	40.0
24	36.96667760724338	39.0	36.0	40.0	31.0	40.0
25	36.89822464184277	39.0	36.0	40.0	31.0	40.0
26	36.769273426316914	39.0	36.0	40.0	30.0	40.0
27	36.71925584729055	39.0	36.0	40.0	30.0	40.0
28	36.56562138358876	39.0	36.0	40.0	30.0	40.0
29	36.470651414082404	39.0	36.0	40.0	30.0	40.0
30	36.37327848018408	39.0	35.0	40.0	30.0	40.0
31	36.24937361853348	38.0	35.0	40.0	30.0	40.0
32	36.15098345801268	38.0	35.0	40.0	30.0	40.0
33	36.034774051875004	38.0	35.0	40.0	29.0	40.0
34	35.90638520051823	38.0	35.0	40.0	29.0	40.0
35	35.71017537446002	38.0	35.0	40.0	28.0	40.0
36	35.66646524889966	38.0	34.0	40.0	28.0	40.0
37	35.56035235861543	38.0	34.0	40.0	28.0	40.0
38	35.66427980952075	38.0	34.0	40.0	28.0	40.0
39	35.72527254695391	38.0	34.0	40.0	28.0	40.0
40	35.69112286957227	38.0	34.0	40.0	28.0	40.0
41	35.66017117525466	38.0	34.0	40.0	28.0	40.0
42	35.57283017384299	38.0	34.0	40.0	28.0	40.0
43	35.52066904456962	38.0	34.0	40.0	28.0	40.0
44	35.37290178676188	38.0	34.0	40.0	27.0	40.0
45	35.22591061003338	38.0	34.0	40.0	27.0	40.0
46	35.08440232957093	38.0	34.0	40.0	27.0	40.0
47	34.95074203458087	37.0	33.0	40.0	26.0	40.0
48	34.86739794592988	37.0	33.0	40.0	26.0	40.0
49	34.671332107330045	37.0	33.0	40.0	26.0	40.0
50	34.51927517598582	37.0	33.0	40.0	26.0	40.0
51	34.28757321335133	37.0	33.0	39.0	26.0	40.0
52	34.04682541103839	36.0	32.0	39.0	25.0	40.0
53	33.82627177257104	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	5.0
11	12.0
12	41.0
13	114.0
14	283.0
15	836.0
16	2037.0
17	4319.0
18	8646.0
19	15382.0
20	25170.0
21	38393.0
22	54532.0
23	74719.0
24	99104.0
25	127837.0
26	161662.0
27	204261.0
28	254394.0
29	314278.0
30	387886.0
31	482734.0
32	601163.0
33	762846.0
34	997645.0
35	1351375.0
36	1924951.0
37	2900039.0
38	4332329.0
39	4305254.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.921004919245576	29.58986525902716	22.985765723039354	23.50336409868791
2	24.536435516878953	26.611445057720545	26.363974976029535	22.488144449370964
3	25.366679140776714	24.69617514144529	25.24919916625189	24.687946551526103
4	23.02109359658234	26.045689618617462	25.031313927240944	25.90190285755925
5	24.79277744911448	29.28546918503716	23.96747406681924	21.95427929902912
6	27.213871498552304	24.745335691475326	25.31120434444847	22.729588465523907
7	23.47262653296726	25.358095470992343	28.754470404041776	22.41480759199862
8	26.534866166796554	24.779176346452555	25.55114570378064	23.134811782970246
9	26.38022116638281	24.87235136151	25.818196638906628	22.929230833200563
10	22.70237596803005	25.324496682010235	29.354622275302372	22.618505074657342
11	22.640020856053297	28.823819045537093	25.707272776675143	22.82888732173447
12	22.603704934189807	25.023352933741894	29.313345527496352	23.059596604571947
13	22.837903262659058	25.24326573024387	25.28983265343258	26.628998353664485
14	22.90291890058217	28.80528284735765	25.40826979976789	22.883528452292293
15	22.909217708625373	28.701753909274935	25.30246629211402	23.086562089985676
16	23.096910866925946	25.048784885824844	25.192268027867904	26.662036219381307
17	26.797064343765065	24.950304257129694	25.32166633525879	22.930965063846447
18	22.937881402089968	24.954148382626652	25.218106520666062	26.889863694617315
19	23.360261766935047	28.632158255699498	25.081318435211408	22.92626154215405
20	26.930358237502936	24.910890392094625	25.20657929026019	22.952172080142248
21	23.258513374263234	28.549167342862237	25.087838524909728	23.1044807579648
22	27.075853498782028	24.821811660699268	25.094219670312977	23.00811517020573
23	23.161046524313605	24.88248400288016	28.96079753613684	22.995671936669396
24	23.187867919347262	28.59059332713333	24.946732874137876	23.27480587938153
25	23.230276805853855	24.878135561052947	24.990881137375357	26.90070649571784
26	23.27657098653743	27.058572945343222	26.540691535019523	23.124164533099826
27	23.400560758590565	24.78314912407458	28.704630570791394	23.111659546543457
28	25.872338599219198	24.711855262448275	26.410670551343312	23.005135586989216
29	25.73717153054037	24.859810352358615	26.368174181391673	23.034843935709343
30	25.626582163834055	24.758833872437197	26.341939440048318	23.27264452368043
31	24.907571167267935	25.198875601011267	26.42862524191746	23.464927989803343
32	24.373582510886028	25.58181637039626	26.624387461502135	23.420213657215573
33	24.22716610039147	25.339533542387894	26.714027116162782	23.719273241057852
34	24.039349436050834	25.357019939226795	26.84087296539237	23.762757659329996
35	24.038469455515386	25.59538659654817	26.811226369692275	23.554917578244165
36	23.842470516020587	25.4565966840275	26.888438229071593	23.81249457088032
37	24.092415864597857	25.416627041812145	26.80815415694571	23.682802936644283
38	24.036534527554405	25.50161463563042	26.838747632286292	23.623103204528885
39	24.218891195707258	25.380856604958936	26.661871544661224	23.738380654672582
40	24.1231379920635	25.42990908720391	26.685250208828133	23.76170271190446
41	23.970947674196005	25.678017283435246	26.752319134667278	23.598715907701468
42	24.13654868958033	25.543606689251803	26.69135346564124	23.628491155526625
43	24.119556316901676	25.5492931131797	26.664367395887496	23.666783174031124
44	24.122556484458205	25.456313649352353	26.746730486354437	23.674399379835005
45	24.063731586793253	25.33558649519088	26.758746594835554	23.841935323180312
46	24.003707651322685	25.612605396966938	26.57873654144389	23.804950410266482
47	23.94267508319161	25.55637927222831	26.74929323668574	23.75165240789434
48	24.064760803793774	25.40617534317182	26.737843197554913	23.79122065547949
49	23.966825660108906	25.7706262291424	26.45737127274209	23.805176838006595
50	24.097165701055275	25.658390115235253	26.578494675448773	23.6659495082607
51	24.03525315238875	25.554470074692336	26.59322277072627	23.817054002192645
52	24.20131216933831	25.63001974861581	26.36680532278098	23.801862759264907
53	24.224356337980044	25.715362422299265	26.301794830942875	23.75848640877782
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17753.0
1	10951.0
2	4149.0
3	3662.0
4	3175.0
5	3271.5
6	3368.0
7	3793.0
8	4877.5
9	5537.0
10	6632.5
11	7728.0
12	10075.0
13	12422.0
14	15654.0
15	18886.0
16	25669.5
17	32453.0
18	42423.0
19	52393.0
20	69418.5
21	86444.0
22	208562.0
23	330680.0
24	268048.0
25	227322.0
26	249228.0
27	277245.0
28	305262.0
29	328302.0
30	351342.0
31	373212.0
32	395082.0
33	434132.5
34	473183.0
35	522294.5
36	571406.0
37	624462.5
38	677519.0
39	735302.5
40	793086.0
41	842771.0
42	945172.5
43	997889.0
44	1069688.5
45	1141488.0
46	1187161.5
47	1232835.0
48	1329439.5
49	1426044.0
50	1468774.5
51	1511505.0
52	1486150.5
53	1460796.0
54	1395336.0
55	1329876.0
56	1248942.5
57	1168009.0
58	1085069.0
59	930175.5
60	858222.0
61	746368.0
62	634514.0
63	545827.5
64	457141.0
65	380032.0
66	302923.0
67	245438.5
68	187954.0
69	150561.0
70	113168.0
71	88332.0
72	63496.0
73	44818.0
74	26140.0
75	19102.0
76	9085.0
77	6106.0
78	4496.0
79	2886.0
80	2440.5
81	1995.0
82	1583.5
83	1172.0
84	784.5
85	397.0
86	268.5
87	140.0
88	114.5
89	89.0
90	67.0
91	45.0
92	34.0
93	16.0
94	9.0
95	7.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.9432248E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.388181546014465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.54110800787983	27.954036400491013
2	16.70085656401512	13.824361668908104
3	6.576261566883244	8.165385228727239
4	2.9692658023140495	4.915700483381847
5	1.5780939574327135	3.2657219603446785
6	0.9708352493814708	2.4108663331602322
7	0.6447824753999094	1.868046190467804
8	0.4764412808199999	1.577523058127505
9	0.33412079493603186	1.244578690720004
>10	1.9975169001150663	14.724514156373086
>50	0.11876063950763147	3.348004237747481
>100	0.06457869726797308	5.5695910885072815
>500	0.02065975923537232	6.0388747893669406
>1k	0.006593065264353824	3.682460225087942
>5k	6.261977363608882E-5	0.19572658562256084
>10k+	6.261977363608882E-5	1.2146089029662757
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	182076	0.9369785729371095	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1905727016246394E-4	0.0	0.0	0.0	5.146085002620387E-6
2	3.2934944016770474E-4	0.0	0.0	0.0	5.146085002620387E-6
3	3.344955251703251E-4	0.0	0.0	0.0	1.0292170005240773E-5
4	3.344955251703251E-4	0.0	0.0	0.0	4.116868002096309E-5
5	3.344955251703251E-4	5.146085002620387E-6	0.0	0.0	4.631476502358348E-5
6	3.344955251703251E-4	5.146085002620387E-6	0.0	0.0	6.689910503406502E-5
7	3.344955251703251E-4	5.146085002620387E-6	0.0	0.0	7.204519003668541E-5
8	3.344955251703251E-4	5.146085002620387E-6	0.0	0.0	9.777561504978734E-5
9	3.447876951755659E-4	5.146085002620387E-6	0.0	5.146085002620387E-6	9.777561504978734E-5
10	3.447876951755659E-4	5.146085002620387E-6	0.0	1.5438255007861158E-5	1.0806778505502811E-4
11	3.499337801781863E-4	5.146085002620387E-6	0.0	1.5438255007861158E-5	1.1835995506026889E-4
12	3.5507986518080667E-4	5.146085002620387E-6	0.0	1.5438255007861158E-5	1.2865212506550967E-4
13	3.6022595018342704E-4	5.146085002620387E-6	0.0	1.5438255007861158E-5	1.4923646507599122E-4
14	3.808102901939086E-4	5.146085002620387E-6	0.0	1.5438255007861158E-5	1.6467472008385237E-4
15	3.8595637519652897E-4	5.146085002620387E-6	0.0	1.5438255007861158E-5	1.7496689008909315E-4
16	3.8595637519652897E-4	5.146085002620387E-6	0.0	1.5438255007861158E-5	1.9555123009957467E-4
17	4.065407152070105E-4	5.146085002620387E-6	0.0	1.5438255007861158E-5	2.0584340010481545E-4
18	4.065407152070105E-4	1.0292170005240773E-5	0.0	1.5438255007861158E-5	2.1613557011005623E-4
19	4.065407152070105E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	2.315738251179174E-4
20	4.1683288521225133E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	2.4186599512315815E-4
21	4.219789702148717E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	2.5730425013101933E-4
22	4.271250552174921E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	2.778885901415009E-4
23	4.4770939522797363E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	2.778885901415009E-4
24	4.7343982024107556E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	2.93326845149362E-4
25	4.888780752489367E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	2.9847293015198244E-4
26	4.991702452541775E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	3.087651001572232E-4
27	5.300467552698998E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	3.1905727016246394E-4
28	5.454850102777609E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	3.2420335516508437E-4
29	5.71215435290863E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	3.344955251703251E-4
30	6.020919453065852E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	3.447876951755659E-4
31	6.381145403249279E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	3.5507986518080667E-4
32	6.947214753537522E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	3.7051812018866785E-4
33	7.513284103825764E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	3.9110246019914935E-4
34	7.873510054009191E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	3.9110246019914935E-4
35	8.233736004192618E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	4.1683288521225133E-4
36	8.593961954376046E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	4.3741722522273283E-4
37	9.05710960461188E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	4.4256331022535326E-4
38	9.777561504978735E-4	1.0292170005240773E-5	0.0	2.0584340010481546E-5	4.580015652332144E-4
39	0.0010446552555319385	1.0292170005240773E-5	0.0	2.0584340010481546E-5	4.7343982024107556E-4
40	0.0011012621905607627	1.0292170005240773E-5	0.0	2.0584340010481546E-5	4.888780752489367E-4
41	0.0012144760606184113	1.0292170005240773E-5	0.0	2.0584340010481546E-5	5.094624152594182E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	79870	0.0	42.9014	1
TCAACGC	81205	0.0	42.37553	12
GGTATCA	80420	0.0	42.3713	8
GTATCAA	80995	0.0	42.288105	9
TGGTATC	80775	0.0	42.118168	7
ACGCAGA	81595	0.0	42.063545	15
ATCAACG	82175	0.0	41.84101	11
CGCAGAG	82475	0.0	41.586235	16
AGAGTAC	81360	0.0	41.34163	19
CAGAGTA	83690	0.0	40.317005	18
GTGGTAT	84590	0.0	40.29921	6
GCAGAGT	84300	0.0	40.145134	17
AGTGGTA	85155	0.0	39.96283	5
TATCAAC	85910	0.0	39.90979	10
CAACGCA	86580	0.0	39.76109	13
AACGCAG	87195	0.0	39.47795	14
GTACATG	51925	0.0	39.41483	22
CAGTGGT	86920	0.0	39.262196	4
GAGTACT	33195	0.0	39.127728	20
GAGTACA	53070	0.0	38.985115	20
>>END_MODULE