FastQCFastQC Report
Thu 26 May 2016
SRR805348_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805348_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12308840
Sequences flagged as poor quality0
Sequence length53
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT664090.5395228144975481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC406950.041.2830812
ACGCAGA458400.041.1403815
AACGCAG463350.040.7160914
AAGCAGT462750.040.662241
CGCAGAG463950.040.61784716
CAACGCA415800.040.40440413
GTATCAA415100.040.3932769
GGTATCA414550.040.3901828
ATCAACG416300.040.37280711
GTGGTAT469300.040.1097376
AGTGGTA469800.039.9520035
AGAGTAC464000.039.9297419
TGGTATC419000.039.88837
GCAGAGT481250.038.80124717
TAAACGC54800.038.76642212
TATCAAC432150.038.75066410
CAGAGTA482000.038.5994818
CAGTGGT489200.038.4348954
GCAGTGG492300.038.3790363
AGCAGTG496050.038.0699542
Produced by FastQC (version 0.11.5)