FastQCFastQC Report
Thu 26 May 2016
SRR805347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17369430
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1057490.608822511734697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC724400.041.36506312
GGTATCA730700.040.92488
GTATCAA731200.040.8518189
ATCAACG733750.040.8219411
ACGCAGA729850.040.7857115
TGGTATC733450.040.7264987
CAACGCA742400.040.36530313
CGCAGAG738800.040.15484616
AAGCAGT653550.040.078191
AGAGTAC725400.039.95388819
AACGCAG754400.039.89766714
TATCAAC754750.039.57714510
GTGGTAT664500.039.4283686
AGTGGTA665750.039.2237325
TATGCCG121500.038.9732515
CAGAGTA751600.038.72997718
GCAGAGT756850.038.66625217
GTACATG528100.038.23811722
CTCGTAT127450.037.92820711
CAGTGGT692150.037.8430984
Produced by FastQC (version 0.11.5)