FastQCFastQC Report
Thu 26 May 2016
SRR805346_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805346_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110861721
Sequences flagged as poor quality0
Sequence length59
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC11966891.079442921511204Illumina Paired End PCR Primer 2 (100% over 55bp)
TACGGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT7571970.6830103241857485Illumina Paired End PCR Primer 2 (100% over 54bp)
GAGACAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT6022490.5432434158224911Illumina Paired End PCR Primer 2 (100% over 54bp)
GCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTG2627840.23703763357597524Illumina Paired End PCR Primer 2 (100% over 57bp)
GCTGAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT2235350.20163406988783802Illumina Paired End PCR Primer 2 (100% over 54bp)
TGGAATCAAGGCTCAGTCCAACTGCACCTCCAGTGACAGATTCCATTCCTGAGATCGGA1908000.17210629447110964No Hit
GAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGC1426030.12863141462507152Illumina Paired End PCR Primer 2 (100% over 58bp)
GAGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT1235340.11143070744860618Illumina Paired End PCR Primer 2 (100% over 56bp)
TAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTG1155870.10426231791945571Illumina Paired End PCR Primer 2 (100% over 57bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAG908700.048.990151
ACGGAGA975300.045.704552
CGGAGAT1098400.040.7753563
GCTGAGA1967300.036.132571
TGAGATC2078600.034.159583
ACAGATC1041550.033.9789244
CTGAGAT2094450.033.9111942
GGAGATC1392800.032.4381454
TACGGAC110600.031.795211
CAGATCG1328150.030.726955
CGTCTTC2577000.027.76278553
GAGATCG2858900.025.1096044
CACGACG160900.025.06712320
CGACGCT133850.024.94583322
CTACACG167450.024.79874417
AGACAGA1454250.024.585732
GAGACAG1443500.024.4934541
GACAGAT1464750.024.2466643
GTCCAAC474650.023.9513316
ACACGAC172100.023.40499719
Produced by FastQC (version 0.11.5)