##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805346_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	110861721
Sequences flagged as poor quality	0
Sequence length	59
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12814384326579	34.0	31.0	34.0	30.0	34.0
2	32.54617884743103	34.0	31.0	34.0	31.0	34.0
3	32.703461567225716	34.0	31.0	34.0	31.0	34.0
4	35.301564387585145	37.0	35.0	37.0	33.0	37.0
5	35.72477863662247	37.0	35.0	37.0	33.0	37.0
6	35.896815430097824	37.0	35.0	37.0	35.0	37.0
7	36.047961883976164	37.0	35.0	37.0	35.0	37.0
8	36.062672416929196	37.0	36.0	37.0	35.0	37.0
9	37.86958688833633	39.0	38.0	39.0	35.0	39.0
10	37.945830445839825	39.0	38.0	39.0	35.0	39.0
11	37.89226436418031	39.0	38.0	39.0	35.0	39.0
12	37.884196953788944	39.0	38.0	39.0	35.0	39.0
13	37.88585823956314	39.0	38.0	39.0	35.0	39.0
14	38.849436894453405	40.0	39.0	40.0	36.0	40.0
15	38.87083722974136	40.0	39.0	40.0	36.0	40.0
16	38.841679672282915	40.0	39.0	40.0	36.0	40.0
17	38.84804950845026	40.0	39.0	40.0	36.0	40.0
18	38.80857356526154	40.0	39.0	40.0	36.0	40.0
19	38.86878000026718	40.0	39.0	40.0	36.0	40.0
20	38.77167163948321	40.0	39.0	40.0	36.0	40.0
21	38.73479497039379	40.0	39.0	40.0	36.0	40.0
22	38.778431096158066	40.0	39.0	40.0	36.0	40.0
23	38.809221038522395	40.0	39.0	40.0	36.0	40.0
24	38.801458873257076	40.0	39.0	40.0	36.0	40.0
25	38.7842628656288	40.0	39.0	40.0	36.0	40.0
26	38.70735023137517	40.0	39.0	40.0	36.0	40.0
27	38.66455853594407	40.0	39.0	40.0	36.0	40.0
28	38.647900017716665	40.0	39.0	40.0	36.0	40.0
29	38.5656413452214	40.0	39.0	40.0	36.0	40.0
30	38.37648731792645	40.0	39.0	40.0	35.0	40.0
31	38.34922855834071	40.0	38.0	40.0	35.0	40.0
32	38.290006611028524	40.0	38.0	40.0	35.0	40.0
33	38.272398901330426	40.0	38.0	40.0	35.0	40.0
34	38.18283735645778	40.0	38.0	40.0	34.0	40.0
35	38.11007096849958	40.0	38.0	40.0	34.0	40.0
36	38.03141044508952	40.0	38.0	40.0	34.0	40.0
37	37.91497467371988	40.0	38.0	40.0	34.0	40.0
38	37.850654717871464	40.0	38.0	40.0	33.0	40.0
39	37.83817656051001	40.0	38.0	40.0	33.0	40.0
40	37.83815916045539	40.0	38.0	40.0	33.0	40.0
41	37.871720302808576	40.0	38.0	40.0	33.0	40.0
42	38.015413354443595	40.0	38.0	40.0	34.0	40.0
43	37.99818887891881	40.0	38.0	40.0	34.0	40.0
44	37.93138309660554	40.0	38.0	40.0	34.0	40.0
45	37.96324414808606	40.0	38.0	40.0	34.0	40.0
46	37.93793368046307	40.0	38.0	40.0	34.0	40.0
47	37.92748533102783	40.0	38.0	40.0	34.0	40.0
48	37.84064593404607	40.0	38.0	40.0	33.0	40.0
49	37.92469960844284	40.0	38.0	40.0	34.0	40.0
50	37.92086373979347	40.0	38.0	40.0	34.0	40.0
51	37.88055309911705	40.0	38.0	40.0	34.0	40.0
52	37.83849725731752	40.0	37.0	40.0	34.0	40.0
53	37.791875619538686	40.0	37.0	40.0	34.0	40.0
54	37.734864895341104	40.0	37.0	40.0	34.0	40.0
55	37.622499528038176	40.0	37.0	40.0	33.0	40.0
56	37.48871629008898	40.0	37.0	40.0	33.0	40.0
57	37.39794157624524	40.0	36.0	40.0	33.0	40.0
58	37.12551266455623	40.0	36.0	40.0	32.0	40.0
59	36.61869159509079	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	14.0
8	57.0
9	138.0
10	186.0
11	306.0
12	438.0
13	730.0
14	1302.0
15	2548.0
16	5230.0
17	9736.0
18	15584.0
19	22545.0
20	31605.0
21	46009.0
22	71505.0
23	114977.0
24	179343.0
25	269845.0
26	387745.0
27	535369.0
28	720233.0
29	940990.0
30	1202302.0
31	1514586.0
32	1893513.0
33	2381105.0
34	3082265.0
35	4170477.0
36	6249127.0
37	1.1399223E7
38	2.9225887E7
39	4.6386795E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.35054319605953	29.267485392906718	22.863626661541726	29.518344749492027
2	22.842065567428815	29.926353930587098	26.300160900442815	20.931419601541275
3	22.28635797562623	26.797369490592697	27.096522342459394	23.819750191321674
4	24.624423790065464	26.91057899056068	26.18243315923266	22.28256406014119
5	24.354308914255444	28.481364636221006	25.92878023244831	21.235546217075232
6	24.089321146295394	27.21984173419065	26.626584662166664	22.064252457347294
7	24.671242475119072	27.262495771646915	26.365736285115037	21.700525468118972
8	22.45346073961814	27.69450602340911	27.779629183277788	22.072404053694964
9	21.866240016245104	25.6150696054953	29.283015550516307	23.235674827743296
10	23.23062258793547	26.951172803821077	25.73729393935712	24.080910668886332
11	25.144922655494405	26.735214583219395	25.76407234378041	22.355790417505787
12	24.112882028955692	27.593684027329868	25.733703881432618	22.559730062281822
13	22.35107463287531	29.563724705302025	25.689470398894493	22.395730262928176
14	23.948512399514346	28.156294813427984	25.457741180113917	22.437451606943753
15	24.092976149991394	27.358663320768763	25.367470165829374	23.18089036341047
16	24.318552658947084	27.75200558180041	25.414020949575555	22.515420809676947
17	24.91021224539713	25.73633869530133	25.814426965282273	23.53902209401927
18	23.53219557181509	26.01496687932528	26.11742153993803	24.3354160089216
19	25.568929242944012	26.01354348449994	25.774064972345144	22.64346230021091
20	24.264160575317064	26.2865394268956	26.930619271191	22.518680726596333
21	22.59755466000749	26.653076222765836	28.43987330847949	22.309495808747187
22	22.506648620401627	25.80505853774361	27.523669779580633	24.164623062274128
23	22.388763926910354	27.474228909002775	25.992999874140505	24.144007289946366
24	23.787818520334895	27.803041231878407	25.69023982588183	22.71890042190487
25	24.62030514572293	25.99201486327278	25.608558791902574	23.779121199101716
26	23.22357597172788	27.189304593241882	25.990603194767292	23.59651624026295
27	24.124871739994006	27.69135615349143	25.729282156823093	22.45448994969147
28	25.687379505862083	26.09620231315009	25.84879951484787	22.367618666139958
29	24.601716222680686	26.954986563847406	26.284786793089744	22.158510420382164
30	22.997461856108114	28.397114636169142	25.79406646591748	22.81135704180526
31	22.657328222425846	27.41298685052887	26.804200522919903	23.125484404125388
32	23.94299381298618	25.999762352597795	27.340879905698017	22.71636392871801
33	24.815132537947882	25.7438381278602	25.63448658712415	23.806542747067763
34	22.944595096083706	26.09857373583439	25.68506400870324	25.271767159378665
35	24.284012332805116	26.050518375048497	25.643867643007273	24.02160164913911
36	24.337905596829042	27.335795193004447	25.583269630100723	22.743029580065784
37	23.817299390472208	27.599392941049505	25.404003064321905	23.17930460415638
38	24.471729065075582	27.27212127619776	25.550656028513213	22.705493630213443
39	23.177217319222386	27.605368854051974	25.53031447166511	23.687099355060525
40	22.635109552376516	26.36713352122686	25.723894363862527	25.273862562534095
41	23.68130384697889	25.837524207296042	26.24843971166567	24.2327322340594
42	24.187734736681563	26.745736700226765	26.1501794654622	22.916349097629467
43	22.58736809615286	27.332123050840966	27.084353128524857	22.996155724481312
44	22.685853848507367	25.724036883749985	27.478176168670522	24.11193309907213
45	22.814945295680552	25.760498522298786	27.159023627280693	24.26553255473997
46	22.544071817178445	25.804588582924847	27.848258823259652	23.80308077663705
47	23.755314063724484	25.990756538949995	26.233651920305295	24.020277477020223
48	24.368567217173183	25.65746115379176	27.131760835644975	22.842210793390084
49	22.989516823394794	26.79378213874201	27.28709127652817	22.929609761335023
50	22.778442163999962	27.274289743346124	27.079249473314597	22.86801861933931
51	24.193099077002422	25.574304407560117	27.29554775719204	22.937048758245417
52	24.61602413695165	25.802289322208882	25.76098741963423	23.820699121205237
53	22.884631206473873	25.612156066023907	25.97346382526391	25.529748902238314
54	23.666222897622166	25.725168022603583	25.887874318674882	24.72073476109937
55	24.099937975886192	25.666739378870005	27.46601597498202	22.767306670261775
56	22.626742372148453	25.57374966242857	27.766592221674063	24.032915743748916
57	22.706237800511865	25.563069691115476	27.12345860118841	24.60723390718425
58	22.846022749367204	25.673274547127043	28.71062230758622	22.770080395919525
59	22.905117989283244	25.813881240396764	27.43131599048512	23.849684779834874
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4733.0
1	3224.0
2	1715.0
3	1557.0
4	1581.0
5	1763.0
6	2054.5
7	2346.0
8	2841.0
9	4308.5
10	5281.0
11	7719.5
12	10158.0
13	14433.5
14	27168.0
15	35627.0
16	55305.0
17	74983.0
18	119914.0
19	260325.0
20	355805.0
21	402030.5
22	448256.0
23	538850.0
24	629444.0
25	745558.0
26	1012851.5
27	1164031.0
28	1367341.5
29	1570652.0
30	1784109.5
31	2216219.0
32	2434871.0
33	2735733.5
34	3036596.0
35	3331643.0
36	3919890.0
37	4213090.0
38	4597374.5
39	4981659.0
40	5370020.0
41	5758381.0
42	6047691.0
43	6603800.0
44	6870599.0
45	6981429.0
46	7092259.0
47	7177193.0
48	7261241.5
49	7260356.0
50	7306745.5
51	7353135.0
52	7052913.5
53	6606597.0
54	6460502.0
55	6932228.5
56	7403955.0
57	6774612.5
58	4722442.0
59	3299614.0
60	2884772.5
61	2469931.0
62	2133014.5
63	1796098.0
64	1494478.0
65	988379.0
66	783900.0
67	616984.0
68	450068.0
69	347542.0
70	201468.0
71	157920.0
72	108360.0
73	58800.0
74	44223.0
75	22231.0
76	14816.0
77	11350.0
78	7884.0
79	6166.5
80	4449.0
81	3501.5
82	2132.0
83	1710.0
84	1150.0
85	590.0
86	474.0
87	338.5
88	319.0
89	345.5
90	372.0
91	512.5
92	791.5
93	930.0
94	902.5
95	875.0
96	858.5
97	426.5
98	11.0
99	6.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.10861721E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.90018764872929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.412051270404646	19.875678230409672
2	17.347289988736048	11.41458191652678
3	7.5057174153366795	7.408185342087364
4	4.095025115383501	5.389083788895059
5	2.502185421066419	4.116118494249995
6	1.657670369926612	3.272259973819442
7	1.1576378670905236	2.6660552139587077
8	0.8744519139395107	2.3015705646720304
9	0.6521921870840222	1.9311520804289537
>10	3.466397777378352	19.764898140533987
>50	0.19231618611834442	4.366059821463483
>100	0.1198776333691322	8.034159358115396
>500	0.011807046163970509	2.594568516131199
>1k	0.005201722088474973	2.998174232598175
>5k	1.142183344532709E-4	0.23171019310269494
>10k+	6.3867579632036E-5	3.6357441330068525
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC	1196689	1.079442921511204	Illumina Paired End PCR Primer 2 (100% over 55bp)
TACGGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT	757197	0.6830103241857485	Illumina Paired End PCR Primer 2 (100% over 54bp)
GAGACAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT	602249	0.5432434158224911	Illumina Paired End PCR Primer 2 (100% over 54bp)
GCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTG	262784	0.23703763357597524	Illumina Paired End PCR Primer 2 (100% over 57bp)
GCTGAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT	223535	0.20163406988783802	Illumina Paired End PCR Primer 2 (100% over 54bp)
TGGAATCAAGGCTCAGTCCAACTGCACCTCCAGTGACAGATTCCATTCCTGAGATCGGA	190800	0.17210629447110964	No Hit
GAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGC	142603	0.12863141462507152	Illumina Paired End PCR Primer 2 (100% over 58bp)
GAGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT	123534	0.11143070744860618	Illumina Paired End PCR Primer 2 (100% over 56bp)
TAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTG	115587	0.10426231791945571	Illumina Paired End PCR Primer 2 (100% over 57bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03438698195926437	5.412147624877662E-6	0.0	1.804049208292554E-6	8.118221437316492E-6
2	0.24878560202037636	6.314172229023938E-6	9.02024604146277E-7	3.608098416585108E-6	8.118221437316492E-6
3	0.6306108129062871	7.216196833170216E-6	4.5101230207313845E-6	4.5101230207313845E-6	1.0824295249755324E-5
4	0.7670465444064323	8.118221437316492E-6	6.314172229023938E-6	9.020246041462769E-6	1.3530369062194155E-5
5	1.9971573416220014	9.922270645609047E-6	6.314172229023938E-6	1.3530369062194155E-5	1.533441827048671E-5
6	3.688519322192373	1.0824295249755324E-5	6.314172229023938E-6	1.533441827048671E-5	1.8040492082925538E-5
7	3.7660582591893914	1.17263198539016E-5	6.314172229023938E-6	1.7138467478779263E-5	1.8942516687071816E-5
8	3.776562335704675	1.3530369062194155E-5	7.216196833170216E-6	1.9844541291218094E-5	2.074656589536437E-5
9	3.7794406962165055	1.3530369062194155E-5	8.118221437316492E-6	1.9844541291218094E-5	2.1648590499510647E-5
10	3.7809921785356373	1.3530369062194155E-5	9.020246041462769E-6	2.074656589536437E-5	2.706073812438831E-5
11	3.7817913723349106	1.6236442874632985E-5	9.020246041462769E-6	2.2550615103656926E-5	3.066883654097342E-5
12	3.7823506275894814	1.6236442874632985E-5	9.020246041462769E-6	2.6158713520242032E-5	3.3374910353412244E-5
13	3.783154331511776	1.7138467478779263E-5	9.922270645609047E-6	2.706073812438831E-5	3.788503337414363E-5
14	3.783814613522011	1.8040492082925538E-5	9.922270645609047E-6	2.7962762728534585E-5	4.239515639487502E-5
15	3.7850206204177543	2.074656589536437E-5	9.922270645609047E-6	3.247288574926597E-5	4.419920560316757E-5
16	3.785875739742485	2.435466431194948E-5	9.922270645609047E-6	3.3374910353412244E-5	4.961135322804523E-5
17	3.787230580697913	2.6158713520242032E-5	1.17263198539016E-5	3.6080984165851076E-5	5.051337783219151E-5
18	3.788761316451149	2.6158713520242032E-5	1.17263198539016E-5	3.788503337414363E-5	5.051337783219151E-5
19	3.7915323360350865	2.7962762728534585E-5	1.17263198539016E-5	3.968908258243619E-5	5.5023500852922895E-5
20	3.804332967192526	2.8864787332680863E-5	1.2628344458047877E-5	4.149313179072874E-5	6.223969768609311E-5
21	3.8063634245764595	2.976681193682714E-5	1.2628344458047877E-5	4.239515639487502E-5	6.494577149853194E-5
22	3.808561658536764	3.066883654097342E-5	1.3530369062194155E-5	4.7807304019752676E-5	6.945589451926333E-5
23	3.8094357203781817	3.247288574926597E-5	1.3530369062194155E-5	4.870932862389896E-5	7.937816516487238E-5
24	3.811219925045183	3.247288574926597E-5	1.4432393666340432E-5	5.2317427040484064E-5	8.569233739389631E-5
25	3.8129536163343523	3.4276934957558526E-5	1.7138467478779263E-5	5.2317427040484064E-5	8.749638660218886E-5
26	3.814018907391849	3.4276934957558526E-5	2.1648590499510647E-5	5.5023500852922895E-5	8.749638660218886E-5
27	3.8174853879455832	3.788503337414363E-5	2.1648590499510647E-5	5.592552545706917E-5	9.02024604146277E-5
28	3.8228713768569405	3.788503337414363E-5	2.1648590499510647E-5	5.8631599269508E-5	9.290853422706653E-5
29	3.8297592367342017	3.878705797828991E-5	2.1648590499510647E-5	6.043564847780056E-5	9.741865724779792E-5
30	3.8409768147113645	3.968908258243619E-5	2.1648590499510647E-5	6.133767308194684E-5	9.741865724779792E-5
31	3.8426798371639928	3.968908258243619E-5	2.34526397078032E-5	6.314172229023939E-5	9.922270645609046E-5
32	3.8458955548777745	3.968908258243619E-5	2.5256688916095754E-5	6.584779610267821E-5	1.0734092789340695E-4
33	3.8527734924843897	4.0591107186582464E-5	2.6158713520242032E-5	6.945589451926333E-5	1.1816522314316228E-4
34	3.8537891721886584	4.419920560316757E-5	2.706073812438831E-5	7.216196833170215E-5	1.1996927235145484E-4
35	3.856409553663703	4.419920560316757E-5	2.7962762728534585E-5	7.396601753999471E-5	1.2628344458047878E-4
36	3.8638593748693473	4.510123020731385E-5	2.7962762728534585E-5	7.667209135243354E-5	1.3349964141364898E-4
37	3.883455859394425	4.510123020731385E-5	2.7962762728534585E-5	7.84761405607261E-5	1.3620571522608783E-4
38	3.885229239766177	4.69052794156064E-5	2.7962762728534585E-5	7.84761405607261E-5	1.407158382468192E-4
39	3.8859346230066194	5.141540243633779E-5	2.7962762728534585E-5	8.118221437316493E-5	1.4251988745511175E-4
40	3.886759975519413	5.321945164463034E-5	2.8864787332680863E-5	8.479031278975004E-5	1.4612798587169688E-4
41	3.8876132907949352	5.5023500852922895E-5	2.976681193682714E-5	8.569233739389631E-5	1.5063810889242825E-4
42	3.8885586125800806	5.592552545706917E-5	2.976681193682714E-5	8.839841120633514E-5	1.569522811214522E-4
43	3.8896040590962864	5.8631599269508E-5	2.976681193682714E-5	8.930043581048141E-5	1.5785430572559848E-4
44	3.890599894259264	6.223969768609311E-5	3.3374910353412244E-5	9.110448501877398E-5	1.6507050255876868E-4
45	3.8914054022307663	6.314172229023939E-5	3.3374910353412244E-5	9.200650962292025E-5	1.7769884701681655E-4
46	3.8922866802690175	6.404374689438566E-5	3.3374910353412244E-5	9.290853422706653E-5	1.795028962251091E-4
47	3.893296947825661	6.584779610267821E-5	3.51789595617048E-5	9.741865724779792E-5	1.8311099464169423E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGAG	90870	0.0	48.99015	1
ACGGAGA	97530	0.0	45.70455	2
CGGAGAT	109840	0.0	40.775356	3
GCTGAGA	196730	0.0	36.13257	1
TGAGATC	207860	0.0	34.15958	3
ACAGATC	104155	0.0	33.978924	4
CTGAGAT	209445	0.0	33.911194	2
GGAGATC	139280	0.0	32.438145	4
TACGGAC	11060	0.0	31.79521	1
CAGATCG	132815	0.0	30.72695	5
CGTCTTC	257700	0.0	27.762785	53
GAGATCG	285890	0.0	25.109604	4
CACGACG	16090	0.0	25.067123	20
CGACGCT	13385	0.0	24.945833	22
CTACACG	16745	0.0	24.798744	17
AGACAGA	145425	0.0	24.58573	2
GAGACAG	144350	0.0	24.493454	1
GACAGAT	146475	0.0	24.246664	3
GTCCAAC	47465	0.0	23.95133	16
ACACGAC	17210	0.0	23.404997	19
>>END_MODULE