##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805345_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	139702936
Sequences flagged as poor quality	0
Sequence length	59
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.169326462831105	31.0	30.0	34.0	16.0	34.0
2	30.39409237612587	31.0	30.0	34.0	25.0	34.0
3	29.19984872758866	31.0	28.0	34.0	16.0	34.0
4	32.45569794610473	35.0	32.0	37.0	19.0	37.0
5	33.85744043346376	35.0	33.0	37.0	28.0	37.0
6	34.95171067843556	37.0	35.0	37.0	32.0	37.0
7	35.368829650079796	37.0	35.0	37.0	33.0	37.0
8	35.58872371873416	37.0	35.0	37.0	33.0	37.0
9	37.372553580405786	39.0	37.0	39.0	34.0	39.0
10	37.41934223916382	39.0	37.0	39.0	34.0	39.0
11	37.28659305342015	39.0	37.0	39.0	34.0	39.0
12	37.365429664269904	39.0	37.0	39.0	34.0	39.0
13	37.39372151777827	39.0	37.0	39.0	34.0	39.0
14	38.36328929407754	40.0	38.0	40.0	35.0	40.0
15	38.321125148006914	40.0	38.0	40.0	34.0	40.0
16	38.322960456607724	40.0	38.0	40.0	34.0	40.0
17	38.33861448695681	40.0	38.0	40.0	34.0	40.0
18	38.35064249472896	40.0	38.0	40.0	35.0	40.0
19	38.263973757860036	40.0	38.0	40.0	34.0	40.0
20	38.31848433020763	40.0	38.0	40.0	34.0	40.0
21	38.278595469174675	40.0	38.0	40.0	34.0	40.0
22	38.303278973320936	40.0	38.0	40.0	34.0	40.0
23	38.18372399847058	40.0	38.0	40.0	34.0	40.0
24	38.252478580693534	40.0	38.0	40.0	34.0	40.0
25	38.2391301568637	40.0	38.0	40.0	34.0	40.0
26	38.162515038338206	40.0	38.0	40.0	34.0	40.0
27	38.07374718309428	40.0	38.0	40.0	34.0	40.0
28	38.03009311844384	40.0	38.0	40.0	34.0	40.0
29	37.93229431484532	40.0	38.0	40.0	34.0	40.0
30	37.8283017115689	40.0	38.0	40.0	33.0	40.0
31	37.790691485539	40.0	38.0	40.0	33.0	40.0
32	37.756209683381314	40.0	38.0	40.0	33.0	40.0
33	37.61786143850262	40.0	38.0	40.0	33.0	40.0
34	37.441517034402196	40.0	37.0	40.0	32.0	40.0
35	37.53178531623702	40.0	37.0	40.0	33.0	40.0
36	37.34775387970372	40.0	37.0	40.0	32.0	40.0
37	37.536822440152584	40.0	37.0	40.0	33.0	40.0
38	37.17671514076125	40.0	37.0	40.0	31.0	40.0
39	37.47735044022267	40.0	37.0	40.0	32.0	40.0
40	37.438505079091534	40.0	37.0	40.0	32.0	40.0
41	37.5224106027378	40.0	37.0	40.0	33.0	40.0
42	37.471865136749884	40.0	37.0	40.0	33.0	40.0
43	37.30334343152244	40.0	37.0	40.0	32.0	40.0
44	37.39315112890684	40.0	37.0	40.0	32.0	40.0
45	37.28084620211561	40.0	37.0	40.0	32.0	40.0
46	37.10162294656427	40.0	37.0	40.0	31.0	40.0
47	37.16605480646448	40.0	37.0	40.0	31.0	40.0
48	37.28624467133604	40.0	37.0	40.0	32.0	40.0
49	37.21600461567966	40.0	37.0	40.0	32.0	40.0
50	37.221410350316475	40.0	37.0	40.0	32.0	40.0
51	37.21890799775318	40.0	37.0	40.0	32.0	40.0
52	37.04087792399724	40.0	37.0	40.0	31.0	40.0
53	37.0469738159261	40.0	36.0	40.0	31.0	40.0
54	37.078103304858246	40.0	36.0	40.0	32.0	40.0
55	36.963148748713486	40.0	36.0	40.0	31.0	40.0
56	36.88779824212141	40.0	36.0	40.0	31.0	40.0
57	36.79622108299857	39.0	36.0	40.0	31.0	40.0
58	36.60030799209546	39.0	35.0	40.0	31.0	40.0
59	36.06225751762297	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	6.0
8	45.0
9	199.0
10	359.0
11	675.0
12	1263.0
13	2051.0
14	3191.0
15	4905.0
16	8023.0
17	13335.0
18	21749.0
19	33906.0
20	54319.0
21	88640.0
22	144538.0
23	229377.0
24	353442.0
25	522555.0
26	747194.0
27	1037082.0
28	1396400.0
29	1844962.0
30	2374681.0
31	2998319.0
32	3683267.0
33	4573887.0
34	5880350.0
35	7911944.0
36	1.1642719E7
37	2.0681115E7
38	4.1722144E7
39	3.1726293E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.315594054515792	27.63842056977242	26.544364106993427	26.501621268718363
2	22.46190588292289	30.456871715280197	26.28594290960356	20.795279492193348
3	23.06770918543902	25.156812022905516	28.50516899659145	23.270309795064005
4	26.038900857459428	26.143442683266155	26.6839187975262	21.13373766174821
5	25.057840588260795	28.408394366171375	25.40873657802009	21.12502846754774
6	22.502111909802668	24.909784286852783	31.350736250811508	21.23736755253304
7	23.0513845464207	27.50738037459714	27.74108555599719	21.700149522984972
8	23.253279372739883	27.344529824340984	27.105985804049244	22.296204998869886
9	22.66393957532861	26.205601720496414	28.29227368564394	22.838185018531036
10	22.793282597868952	27.390417192091082	26.359000071408662	23.4573001386313
11	23.064347767179353	27.51278326748981	26.531561942262975	22.89130702306786
12	23.03366050946846	27.28547952635727	26.524722429598757	23.156137534575507
13	22.9329919021888	27.463600335500466	26.531970666672315	23.07143709563842
14	23.489842045982485	27.332634584000438	26.32772442234142	22.84979894767566
15	23.13099203584383	26.9786226969489	26.392854048536247	23.49753121867102
16	23.2078014452037	27.09869533450607	26.507126521664514	23.18637669862572
17	23.593401072114904	26.822838569405583	26.375600295186352	23.208160063293157
18	22.838502835760018	26.94655608383205	26.93409893690423	23.280842143503698
19	23.543688444743925	26.951195213248774	26.201106467798212	23.304009874209086
20	23.40119036582023	27.05224248114585	26.615301055662854	22.93126609737107
21	23.20106858742038	27.24068877120807	26.666927028648846	22.8913156127227
22	22.916892025805385	26.795654459259183	26.812039225861366	23.475414289074067
23	23.52441898572554	26.905350077968297	26.74421459546133	22.826016340844834
24	23.24703469367315	26.603619125084098	26.60240154151091	23.546944639731837
25	23.2582098346165	26.875793791477655	26.494380189690503	23.37161618421534
26	23.731466889142546	26.389882027962535	26.83570229332904	23.042948789565884
27	23.91810720427522	26.473049213511164	26.641579673028488	22.967263909185128
28	23.884382071970197	26.372024851360322	26.784359063148106	22.959234013521375
29	23.507110115423774	26.456544191741255	26.881129398740768	23.155216294094206
30	23.389607932076675	26.51623441901035	26.86189429834173	23.232263350571245
31	23.377911685406524	26.531607753755438	26.744543865563426	23.34593669527461
32	23.362707996344472	26.675067874020915	26.710654814011924	23.251569315622685
33	23.614927462941797	26.35100167114598	26.839495341744286	23.194575524167938
34	23.738604892312356	26.558229241510002	26.56012039718335	23.143045468994295
35	23.623043255153924	26.519509224917076	26.622835614564323	23.23461190536468
36	23.949614774023072	26.388595011346077	26.608643357359362	23.05314685727149
37	23.45736670845629	26.69417341379282	26.709151624415394	23.139308253335493
38	24.02926091689297	26.66097153462831	26.50620456537864	22.803562983100083
39	23.502568335428542	27.01177733301181	26.390652233679614	23.095002097880034
40	23.4262270622573	27.101776157374385	26.38424506697554	23.087751713392766
41	23.302342049561506	27.08718877604691	26.558793295510984	23.051675878880598
42	23.37906055174102	26.919021945251032	26.53263063848565	23.169286864522302
43	23.554511409838945	26.991751268563174	26.33880722449527	23.11493009710261
44	23.37113945837187	26.95205990516907	26.542902434061943	23.13389820239712
45	23.351089056567858	26.829271505074164	26.566211894072143	23.253427544285827
46	23.68602546763942	26.87045532099626	26.31181208675529	23.131707124609036
47	23.423428982194046	26.856688251705744	26.56664925066428	23.153233515435925
48	23.364108110082952	26.774666353468763	26.495446022694896	23.36577951375338
49	23.65286438933538	26.751986801479966	26.385459071525887	23.20968973765877
50	23.519957375842125	26.810026383411156	26.551082648685348	23.11893359206137
51	23.530186223144227	26.597032291433013	26.577552385871115	23.295229099551637
52	23.695317326759692	26.722662435669925	26.376898048871354	23.205122188699026
53	23.45296164713389	26.742040696982915	26.525797568062565	23.27920008782063
54	23.418209335271236	26.66451261983499	26.488611520662676	23.4286665242311
55	23.347892273359236	26.811792273284794	26.48144918013749	23.35886627321848
56	23.43353972174214	26.78545209672616	26.51178426200005	23.269223919531655
57	23.446747031859086	26.696904924031088	26.435687793991676	23.42066025011815
58	23.408091437677445	26.78909912100917	26.401558232104726	23.40125120920866
59	23.443475804975208	26.779951854411994	26.42635370240179	23.35021863821101
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3882.0
1	2833.5
2	1785.0
3	2125.0
4	2644.0
5	2823.0
6	3420.5
7	4018.0
8	4423.0
9	6352.5
10	7877.0
11	11082.0
12	14287.0
13	19765.5
14	36679.0
15	48114.0
16	73591.5
17	99069.0
18	156183.0
19	328872.5
20	444448.0
21	515068.5
22	585689.0
23	721246.0
24	856803.0
25	1001222.0
26	1342728.5
27	1539816.0
28	1791862.5
29	2043909.0
30	2312709.0
31	2858424.5
32	3135340.0
33	3511199.0
34	3887058.0
35	4274495.0
36	5072241.5
37	5482551.0
38	5983983.5
39	6485416.0
40	7000258.5
41	7515101.0
42	7934607.0
43	8721660.5
44	9089208.0
45	9274412.0
46	9459616.0
47	9589924.0
48	9737084.5
49	9753937.0
50	9672529.5
51	9591122.0
52	9294489.5
53	8572878.5
54	8147900.0
55	7598613.5
56	7049327.0
57	6306882.0
58	4869221.5
59	4174006.0
60	3656167.5
61	3138329.0
62	2702976.5
63	2267624.0
64	1882251.0
65	1227669.5
66	958461.0
67	748926.0
68	539391.0
69	416017.0
70	235366.5
71	178090.0
72	122871.0
73	67652.0
74	50101.5
75	24358.0
76	16165.0
77	12513.0
78	8861.0
79	6958.0
80	5055.0
81	4098.0
82	2514.5
83	1888.0
84	1266.0
85	644.0
86	502.0
87	284.0
88	208.0
89	180.5
90	153.0
91	120.5
92	66.0
93	44.0
94	35.0
95	26.0
96	21.5
97	12.0
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.39702936E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.19523780113064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.119605009416766	20.55804189807051
2	17.68134411629045	12.092355334003479
3	7.491258727252107	7.68496120824543
4	4.075994875728674	5.575184562069277
5	2.425076866802165	4.146304007316042
6	1.6335107034160388	3.3514972172402167
7	1.17906754699993	2.822294660728087
8	0.8782362558502099	2.402519809149803
9	0.6646888316055	2.045627339445517
>10	3.5355489576977295	21.271742971000712
>50	0.1860610555177287	4.326572744199707
>100	0.1130865791322062	8.102811023294908
>500	0.011653693027270956	2.6588829586114713
>1k	0.004787478923156137	2.7589754479348114
>5k	6.870149771864922E-5	0.14523718297956237
>10k+	1.060084256255614E-5	0.056991635710336344
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001033621798757329	7.158045697765435E-7	0.0	2.863218279106174E-6	1.431609139553087E-6
2	0.0519266109052998	2.1474137093296305E-6	0.0	4.294827418659261E-6	2.1474137093296305E-6
3	0.056078993214573526	3.579022848882718E-6	0.0	7.87385026754198E-6	2.863218279106174E-6
4	0.11461248029891083	3.579022848882718E-6	2.1474137093296305E-6	1.0737068546648154E-5	2.863218279106174E-6
5	0.38243648651736284	4.294827418659261E-6	2.863218279106174E-6	1.2884482255977784E-5	5.010631988435805E-6
6	0.46548556431197696	4.294827418659261E-6	2.863218279106174E-6	1.4316091395530871E-5	1.1452873116424697E-5
7	0.4658992993533078	4.294827418659261E-6	2.863218279106174E-6	1.4316091395530871E-5	1.6463505104860503E-5
8	0.4664519004811753	5.726436558212348E-6	2.863218279106174E-6	1.5031895965307414E-5	2.1474137093296308E-5
9	0.4665120280650365	7.158045697765436E-6	2.863218279106174E-6	1.9326723383966678E-5	2.5768964511955568E-5
10	0.46661510392308436	7.158045697765436E-6	2.863218279106174E-6	2.0758332523519763E-5	3.006379193061483E-5
11	0.46664588351958475	7.158045697765436E-6	2.863218279106174E-6	2.2905746232849394E-5	3.364281477949755E-5
12	0.4666659260475385	7.158045697765436E-6	2.863218279106174E-6	2.6484769081732113E-5	3.579022848882718E-5
13	0.46669742144860865	7.87385026754198E-6	2.863218279106174E-6	2.7916378221285198E-5	4.2948274186592616E-5
14	0.466747527768493	7.87385026754198E-6	2.863218279106174E-6	2.8632182791061743E-5	5.010631988435805E-5
15	0.46682340305288933	7.87385026754198E-6	2.863218279106174E-6	3.0779596500391376E-5	5.010631988435805E-5
16	0.4669894697130775	8.589654837318522E-6	2.863218279106174E-6	3.5074423919050637E-5	5.8695974721676575E-5
17	0.46732160303345377	8.589654837318522E-6	2.863218279106174E-6	3.650603305860372E-5	6.72856295589951E-5
18	0.46780691853176226	1.002126397687161E-5	3.579022848882718E-6	3.8653446767933356E-5	7.086465240787781E-5
19	0.4699371529314173	1.002126397687161E-5	4.294827418659261E-6	4.151666504703953E-5	7.659108896609016E-5
20	0.4710266074866172	1.0737068546648154E-5	4.294827418659261E-6	4.43798833261457E-5	8.23175255243025E-5
21	0.4726622209285566	1.0737068546648154E-5	4.294827418659261E-6	4.9390515314581506E-5	8.87597666522914E-5
22	0.4735891878464172	1.0737068546648154E-5	4.294827418659261E-6	5.225373359368768E-5	9.734942148960993E-5
23	0.47437442545946207	1.0737068546648154E-5	4.294827418659261E-6	5.6548561012346944E-5	1.0092844433849264E-4
24	0.47596136419065666	1.0737068546648154E-5	4.294827418659261E-6	6.012758386122966E-5	1.0307585804782227E-4
25	0.47698782794371625	1.1452873116424697E-5	4.294827418659261E-6	6.513821584966546E-5	1.0379166261759882E-4
26	0.4782755603647442	1.2168677686201241E-5	4.294827418659261E-6	6.656982498921855E-5	1.0808649003625808E-4
27	0.4798174034080429	1.2168677686201241E-5	5.010631988435805E-6	6.943304326832473E-5	1.1238131745491734E-4
28	0.48118745335459523	1.3600286825754329E-5	5.010631988435805E-6	7.372787068698399E-5	1.1452873116424697E-4
29	0.483209601264214	1.3600286825754329E-5	5.010631988435805E-6	8.160172095452597E-5	1.1953936315268278E-4
30	0.48495831182817806	1.5031895965307414E-5	5.010631988435805E-6	8.303333009407905E-5	1.2454999514111857E-4
31	0.4870742301364375	1.6463505104860503E-5	5.010631988435805E-6	8.518074380340868E-5	1.2741321342022477E-4
32	0.4898959317504966	1.7179309674637044E-5	7.158045697765436E-6	8.87597666522914E-5	1.3815028196687292E-4
33	0.49356085114775256	1.7179309674637044E-5	7.158045697765436E-6	9.090718036162103E-5	1.4602413223441487E-4
34	0.49600818697181853	1.8610918814190133E-5	7.158045697765436E-6	9.663361691983338E-5	1.4745574137396797E-4
35	0.5000288612402534	2.004252795374322E-5	7.158045697765436E-6	9.949683519893956E-5	1.5962441906016922E-4
36	0.5021948858683972	2.1474137093296308E-5	7.158045697765436E-6	1.0307585804782227E-4	1.6892987846726427E-4
37	0.5052971828738088	2.218994166307285E-5	7.158045697765436E-6	1.052232717571519E-4	1.7465631502547663E-4
38	0.5076715066317575	2.218994166307285E-5	7.158045697765436E-6	1.0593907632692845E-4	1.7966694701391242E-4
39	0.5098697424655413	2.3621550802625938E-5	7.158045697765436E-6	1.0737068546648153E-4	1.8181436072324205E-4
40	0.5123550159318054	2.5053159942179024E-5	7.158045697765436E-6	1.1166551288514079E-4	1.8324596986279515E-4
41	0.5148281207203834	2.5053159942179024E-5	7.158045697765436E-6	1.1810775401312968E-4	1.904040155605606E-4
42	0.5174057329761488	2.7200573651508657E-5	7.158045697765436E-6	1.2025516772245931E-4	1.968462566885495E-4
43	0.52028469895579	2.7916378221285198E-5	7.87385026754198E-6	1.2240258143178894E-4	2.0829912980497418E-4
44	0.5227162870793209	2.7916378221285198E-5	7.87385026754198E-6	1.2383419057134204E-4	2.154571755027396E-4
45	0.5250734315275951	2.9347987360838287E-5	7.87385026754198E-6	1.266974088504482E-4	2.2189941663072852E-4
46	0.5274320075850089	2.9347987360838287E-5	7.87385026754198E-6	1.3027643169933093E-4	2.2619424404938778E-4
47	0.5296875077843747	3.0779596500391376E-5	7.87385026754198E-6	1.3170804083888403E-4	2.319206806076001E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGATA	29305	0.0	42.510323	1
ACGGTAA	34870	0.0	41.8969	2
GCCGATA	18815	0.0	41.40845	1
CGGTAAG	24135	0.0	40.504868	3
GCCGATT	21750	0.0	39.54896	1
GCGGATC	34850	0.0	39.327972	1
GCGGATT	36110	0.0	38.63819	1
ACGGTAG	23045	0.0	36.64808	2
GACGGTA	40555	0.0	35.92578	1
GCCGATC	23825	0.0	35.592865	1
ACGGTTA	25735	0.0	33.97066	2
CACGGTA	23120	0.0	32.391434	1
TATGGTA	58745	0.0	32.181633	1
GATACTA	49905	0.0	30.904718	1
CGGATAG	15750	0.0	30.74	2
AACGGTT	16520	0.0	30.590494	1
AACGGTA	17330	0.0	30.491053	1
GACGGTT	45920	0.0	29.806728	1
CCGATTG	13655	0.0	28.566824	2
ACGGTTG	37800	0.0	28.322752	2
>>END_MODULE